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Root-knot nematode-induced gall biogenesis depends on cell cycle hyperactivation : Functional studies of new players of the mitotic and endocycle in giant-feeding cells Danila Cabral Do Nascimento

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Danila Cabral Do Nascimento. Root-knot nematode-induced gall biogenesis depends on cell cycle hyperactivation : Functional studies of new players of the mitotic and endocycle in giant-feeding cells. Molecular biology. COMUE Université Côte d'Azur (2015 -2019), 2019. English. NNT : 2019AZUR6001. tel-03092244 É preciso força pra sonhar e perceber que a estrada vai além do que se vê.

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In situ analysis in syncytia helped us to find out if E2Fa or E2Fb are preferentially expressed in mitotic or endocycling cells (

). In syncytia mitosis is seen only in dividing cells prior to incorporation to the feeding site and only endoreduplication occurs in the feeding cell self. Herein, in situ E2Fa localization in syncytia showed a strong hybridization signal overall but more intense signal was seen in dividing cells neighboring the feeding site ( et al., 2015). Basically, by engaging in a prolonged and intimate interaction with their host plants. This biotrophic relationship provoke complex alterations in host cell morphology and function and is cell cycle dependent (de Almeida-Engler et al., 1999;Vieira et al., 2013;Ramos et al., 2017).

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SUMMARY

  considered the most harmful plant-pathogens causing huge yield losses for the world agriculture (Singh

  Danila (1), SIMOES DE SIQUEIRA Kercya M.(1), CG FERREIRA Paulo (2)(3), ENGLER Gilbert(1), S HEMERLY Adriana(2)(3), DE ALMEIDA ENGLER Janice(1) (1) INRA, Université Cote D'Azur, CNRS, UMR 1355-7254 Institut Sophia Agrobiotech, 06900 Sophia Antipolis, France (2) Instituto de Bioquímica Médica, Universidade Federal do Rio de Janeiro, Rio de Janeiro, Brazil (3) Laboratório de Biologia Molecular de Plantas, Instituto de Pesquisas do Jardim Botânico do Rio de Janeiro, DIPEC, Rio de Janeiro, Brazil (Corresponding authors: danila.cabral-do-nascimento@inra.fr, janice.de-almeida@inra.fr ) dopsi Gall development-Meloidogyne incognita •Morphological and nuclear analysis shows that lack of ABAP and ABAP1-interactor protein will induce mitotic activity in galls (giant cells and neighboring cells) most likely due to a decreased inhibitory function on mitosis caused by the lack of these cell cycle proteins in galls. •Morphological analysis shows that overexpression of ABAP and ABAP1-interactor will inhibit giant cell development and NC division leading to smaller galls and consequently less reproduction . •Cleared galls under ectopic ABAP and ABAP1-interactor expression show decreased mitosis in the gall possibly due to the importance of these gene complex ABAP1-AIP10 for the mitotic activity of nematode feeding sites. show small GCs presenting cell wall stubs and increased number of vacuoles. Galls were smaller due to the decreased mitotic activity. Ectopic ABAP1-interactor expression showed significantly smaller GCs compared to wild-type. Less and disorganized NC suggest a inhibition in mitosis during gall developement. abap1/ABAP1 and no aip10 galls presented more NC and GCs with more nuclei. in ABAP OE , abap1/ABAP1, ABAP1-interactor OE and aip10 showed nuclear morphology changes (density and shape) in giant cells when compared with wild-type. Nematodes Infection Tests Nuclear Morphology in cleared galls Nematode infection tests in lines downregulated for ABAP1 (abap1/ABAP1 since abap1 is lethal) show similar number of galls and reproduction level decreased compared to the wild-type . Nematode infection tests of abap1-interactor showed decreased number of egg masses and galls compared to wild-type.