Kashif Shoaib

Zaeem Saeed

Saeed Noman

Adnan Ahmed Chatha

Amer Dogar

Taj Yousaf

Syed Asim Muhammad

Aamer Ali

Khurram Baqai

Husain Khurshid

Kashif Pervez

Naeem Mehmood

Tenzila Iqbal

Batool Khan

Bukhari

Muhammad Kashif Zahoor

Ago Cyc

Trh

Keywords: ago, slimb, CG9144 Slimb Arm, Ci, Cactus, Paired

for their moral support throughout my course of study

Acknowledgement

I feel much honor to express my ineffable gratitude and profound indebtedness to my venerable supervisor Dr. Jacques Montagne for his exceptional guidance, sagacious suggestions and precept advice in every aspect, hour-lasting thought-provoking discussion and exploratory criticisms, from bench work to manuscript writing and sympathetic attitude throughout the course of study. I am thankful for having an opportunity to work with such a dedicated scientist, who has influenced and enhanced my passion for science.

I am heartily grateful to the members of my thesis jury committee; Dr. Laurent Théodore, Dr. François Leulier, Dr. Frédérique Perronet, Dr. Mario Pende and Dr.

François Rouyer; for their precious time, valuable comments and suggestions for this research work. With deep emotion of gratitude, I offer my appreciation to Dr. Pierre Capy, Director de l"ecole doctoral; for his encouragement to complete my research work.

I have no words to express my feelings to Dr. Jean Philippe Parvy; many thanks for his interest, guidance, research expertise, valuable suggestions and productive discussions during the study. It is a pleasure to thank Brigitte Maroni, and Mickaël Poidevin who made profound efforts to make this thesis possible. I am also very much indebted to Laura Napal, Caroline Lecerf and Thomas Rubin for sharing with me the cheerful as well as gloomy moments and particularly their exclusive cooperation and necessary help during the study. A deep appreciation goes to Souhir, Peng, Fabien, Ekram, Diana, Laïtita, Yousra, Haela, Rabih, Demian and all the past and present lab members for their help and support. I offer also my deep regards to all the people of CGM who supported me in any respect during the completion of the project. Table 1 -

List of Figures

List of Tables

Abstract

he evolutionary conserved insulin and nutrient signaling network regulates growth and metabolism. Nutrients are directly utilized for growth or stored, mostly as triglycerides. In Drosophila, activation of insulin/nutrient signaling in the fat body (the fly equivalent of liver and adipose tissue), causes an increase in fat stores composed of several small-size lipid droplets (LDs). Conversely, fasting produces an increase in LD size and a decrease in fat contents. The TOR kinase and its substrate S6 kinase (S6K) play a central role in this response, and particularly in Drosophila, they have been shown

to orchestrate cell-autonomous and hormone-controlled growth. However, despite extensive research studies on different model organisms (mouse, fly, worm) to decipher the molecular and physiological functions of S6K, nothing is known about how its degradation is regulated.

Taking advantage of the inducible RNA interfering (RNAi) library from NIG (Japan), we have performed three genetic screens to identify novel regulators of steroidogenesis, lipid metabolism and dS6K-dependent growth. First, RNAi lines were screened in the ring gland; an organ that controls the progression of the developmental steps by producing the steroid hormone ecdysone. Out of 7,000 genes screened, 620 positive candidates were identified to produce developmental arrest and/or overgrowth phenotypes. Then, we challenged 4,000 genes by RNAi screening able to recapitulate the larger sized LD phenotype as obtained upon starvation, leading to the identification of 24 potential candidates. Finally, the RNAi lines were screened for their ability to enhance a growth phenotype dependent of the Drosophila S6K (dS6K). Out of 7,000 genes screened, 45 genes were identified as potential negative regulators of dS6K. These genes were further used to design a novel protein-protein interaction network centered on dS6K through the available data from yeast-2-hybrid (Y2H) assay. The most potent interactors were then analyzed by treatment of cultured S2 cells with the corresponding double strand RNA (dRNA). Western blotting thus, allowed us to discriminate between the gene products that regulate dS6K levels versus those that regulate its phosphorylation, as a hallmark for

T Introduction 1.1 -Cellular signaling regulating growth and metabolism

The fundamental cellular activities in eukaryotes are organized in a coordinated fashion through complex interrelating networks of cellular signaling which regulate growth and development process. One of the major, still incompletely understood problems in biology is how the final size and organ proportions of an organism are achieved [START_REF] Mirth | Size assessment and growth control: how adult size is determined in insects[END_REF]. The highly sophisticated and synchronized tissue growth requires crosstalk between energy producing and energy consuming tissues of the animal. The final body size of an individual is determined by genetic and environmental factors. All animal species have their genetically determined optimal size, which can vary tremendously even between closely related species. Among the most important environmental factors that contribute to determining the growth rate and final size of animals is nutrition [START_REF] Hietakangas | Regulation of tissue growth through nutrient sensing[END_REF]. In multicellular animals, however, each cell also needs to receive information about the nutritional status of the whole animal. This kind of systemic regulation through signaling pathways allows the animal to coordinate growth between tissues and adjust growth rates and consumption of energy stores during periods of nutrient limitation. The insulin/IGF pathway regulates growth mainly through the phosphatidylinositol 3-kinase (PI3K)/PKB protein kinase signaling pathway, which converges with the cellular nutrient-sensing pathways mainly at TOR (target of rapamycin) [START_REF] Hietakangas | Regulation of tissue growth through nutrient sensing[END_REF]. As the main nodal point in nutrient sensing in all eukaryotes is the protein kinase TOR, it regulates and adjusts cell growth, largely by determining the rate of protein biosynthesis [START_REF] Schmelzle | TOR, a central controller of cell growth[END_REF]. The TOR kinase is present in two complexes; TORC1 and TORC2 which have important roles in growth control, but they act in different ways. Two key substrates of TORC1 are S6 kinase (S6K) and eukaryotic initiation factor 4E binding protein (4E-BP)

regulating mainly growth and metabolism of an organism [START_REF] Martin | The expanding TOR signaling network[END_REF][START_REF] Kim | GbetaL, a positive regulator of the rapamycin-sensitive pathway required for the nutrient-sensitive interaction between raptor and mTOR[END_REF][START_REF] Loewith | Two TOR complexes, only one of which is rapamycin sensitive, have distinct roles in cell growth control[END_REF].

At the cellular level, the biological processes of cell proliferation, cell growth, cell differentiation, autophagy and apoptosis are all tightly coupled to appropriate alterations in metabolic status. In the case of cell growth and cell proliferation, this requires redirecting metabolic pathways to provide the fuel and basic components for new cells.

Ultimately, the successful co-ordination of cellular biology with cellular metabolism underscores a wide range of multicellular processes during the course of development of an organism [START_REF] Sethi | Wnt signaling and the control of cellular metabolism[END_REF]. Therefore, the processes that govern growth regulation are probably closely coupled to ones regulating metabolism. The evolutionary conserved insulin/TOR signaling pathway plays its role the regulation of growth as well as glucose and lipid metabolism [START_REF] Colombani | A nutrient sensor mechanism controls Drosophila growth[END_REF][START_REF] Arquier | Fly foie gras: modeling Fatty liver in Drosophila[END_REF][START_REF] Wullschleger | TOR signaling in growth and metabolism[END_REF][START_REF] Ducharme | Lipid droplets in lipogenesis and lipolysis[END_REF]. Different components of the insulin/TOR signaling pathway are being individually delineated in a range of experimental systems, i.e. Drosophila, mouse and yeast. Therefore, our understanding of how different components of cell growth and metabolism machinery are regulated and how they integrate with each other still needs a lot of efforts of elucidation.

S6 kinase is an evolutionary conserved determinant of growth through increased protein biosynthesis. Although several studies have shown how S6 kinase is regulated; however with particular context to the ultimate fate of S6 kinase, nothing is known about its degradation. Moreover, the regulation of metabolism particularly lipid metabolism which provides energy and macromolecules to the growing cells still needs detailed insights. In this thesis, we have used the model organism Drosophila melanogaster in order to investigate the regulation of growth and lipid metabolism. Below here, a concise introductory note on cellular signaling pathways is discussed briefly.

-Cellular signaling

For the survival of an organism, it needs to react to an alternating environment and adjusts its behavior to meet the prevailing conditions. The reaction of a metazoan animal is the result of a perception event which is followed by a coordinated response of various specialized cell types. This response ultimately leads to a change in the animal behavior.

This coordinated action of metazoan operations relies on communication between cells.

Hence, individual cells are faced with the same requirements as the organism as a whole, they need to receive information and respond in an appropriate manner. For the purpose of cellular communication, cells have evolved signaling mechanisms to elicit and receive information through different interactions. These mechanisms, i.e. cellular signaling pathways; collect and integrate information about extracellular conditions, are important for survival, growth and the differentiation [START_REF] Sethi | Wnt signaling and the control of cellular metabolism[END_REF].

Through information collection from its external milieu, a cell is able to respond to changes in nutrient and growth factor composition and abundance by modulating its protein post-translational modifications profile. These alterations can lead to responses such as regulation of growth and metabolism, cell division, cell death, and modulation of cellular cytoskeleton. The response of a given cell to a given signal is dictated by its repertoire of molecular signal transduction mechanisms. These mechanisms consist of different receptors and intracellular signaling pathway components such as kinases which are all coordinated into a signaling cascade to govern a given biological process [START_REF] Marshall | Role of insulin, adipocyte hormones, and nutrient-sensing pathways in regulating fuel metabolism and energy homeostasis: a nutritional perspective of diabetes, obesity, and cancer[END_REF][START_REF] Hietakangas | Regulation of tissue growth through nutrient sensing[END_REF][START_REF] Sethi | Wnt signaling and the control of cellular metabolism[END_REF].

-Signal transduction pathways

A signaling pathway comprises of several intracellular transducers. These are typically kinases and phosphatases which by incorporating or removing a phosphate group alter the physiochemical properties of a substrate protein or a second messenger such as inositol-lipids [START_REF] Graves | Protein phosphorylation and signal transduction[END_REF][START_REF] Vanhaesebroeck | Synthesis and function of 3-phosphorylated inositol lipids[END_REF]. A signaling pathway may consist of several kinases which sequentially phosphorylate each other in a specified order. For instance, PI3K/TOR signaling pathway comprises different events of sequential phosphorylation and then downstream triggers S6 kinase signaling [START_REF] Volarevic | Role of S6 phosphorylation and S6 kinase in cell growth[END_REF][START_REF] Oldham | Genetic and biochemical characterization of dTOR, the Drosophila homolog of the target of rapamycin[END_REF].

A characteristic consequence of a protein phosphorylation event is a conformational change in its three dimensional structure, which exposes its catalytic cleft to interact with its substrates. A good example of such a regulation is the activation of Protein kinase B (PKB/Akt) and S6 kinase (S6K) [START_REF] Dutil | Dual role of pseudosubstrate in the coordinated regulation of protein kinase C by phosphorylation and diacylglycerol[END_REF][START_REF] Calleja | Intramolecular and intermolecular interactions of protein kinase B define its activation in vivo[END_REF][START_REF] Volarevic | Role of S6 phosphorylation and S6 kinase in cell growth[END_REF][START_REF] Oldham | Genetic and biochemical characterization of dTOR, the Drosophila homolog of the target of rapamycin[END_REF][START_REF] Montagne | S6K integrates nutrient and mitogen signals to control cell growth[END_REF]. In addition to kinases and phosphatases, a signaling pathway may contain several other adapter proteins which may facilitate protein-protein interactions and enzymatic activity [START_REF] Pratt | Transcriptional regulatory network analysis during epithelialmesenchymal transformation of retinal pigment epithelium[END_REF]. For example, PKB/Akt is known to associate with more than ten different proteins having a role in its stabilization, kinase activity and intracellular transport [START_REF] Du | Regulation of the Akt kinase by interacting proteins[END_REF]Radimerski et al., 2002b;[START_REF] Potter | Akt regulates growth by directly phosphorylating Tsc2[END_REF][START_REF] Rintelen | PDK1 regulates growth through Akt and S6K in Drosophila[END_REF].

PKB is thought to have an activation of mTORC1 and ultimately makes a link between nutrients and insulin signaling pathways impinging on TOR kinase [START_REF] White | Insulin signaling in health and disease[END_REF][START_REF] Oldham | Genetic and biochemical characterization of dTOR, the Drosophila homolog of the target of rapamycin[END_REF].

Although there are multiple developmental outputs, there are a limited number of signaling pathways controlling cell fate decisions, including Wnt, TGF-β, Hedgehog, receptor tyrosine kinase, nuclear receptor, Jak/STAT and Notch pathways [START_REF] Lodish | Molecular Cell Biology[END_REF]. Each of these signaling pathways is used over and over throughout the development of an organism to activate the target gene expression corresponding to various developmental contexts. Mechanisms of signal transduction utilized by each of these pathways are very different from one another, but the output is the same, the activation of target genes [START_REF] Barolo | Three habits of highly effective signaling pathways: principles of transcriptional control by developmental cell signaling[END_REF].

A primary role of signaling pathways is to regulate a particular cellular process. In spite of different signaling pathways, we know less concerning the signaling pathways that control cell growth, specifically those associated with the regulation of protein synthesis [START_REF] Volarevic | Role of S6 phosphorylation and S6 kinase in cell growth[END_REF]). The main key player which regulates growth and metabolic processes is TOR kinase [START_REF] Bryk | MAP4K3 regulates body size and metabolism in Drosophila[END_REF]. The downstream effecter of TOR, 4E-BP regulates organismal lipid homeostasis rather than growth (Teleman et al., 2005a). Lipid metabolism is essential for growth and generates much of the energy needed for normal developmental process as well as during periods of food deprivation.

The regulation process of lipid metabolism is partly monitored by TOR signaling pathway which also responds to cellular levels of amino acids [START_REF] Laplante | An emerging role of mTOR in lipid biosynthesis[END_REF][START_REF] Bryk | MAP4K3 regulates body size and metabolism in Drosophila[END_REF]. Especially from growth point of view, the whole activities centres on the downstream target of TOR; the S6 kinase signaling and then ultimately upon translational machinery. In parallel, a great deal of information has been accumulated concerning the signaling pathways and the regulatory phosphorylation sites involved in controlling S6K activation [START_REF] Meyuhas | Physiological roles of ribosomal protein S6: one of its kind[END_REF]. One main substrate of S6 kinase is ribosomal protein S6 (RPS6), which has been implicated in the protein synthetic apparatus. [START_REF] Hietakangas | Regulation of tissue growth through nutrient sensing[END_REF][START_REF] Volarevic | Role of S6 phosphorylation and S6 kinase in cell growth[END_REF].

Below are briefly introduced central signaling systems involved in the regulation of growth and metabolism important for this work.

-Target of rapamycin (TOR)

In the 1970s, a bacterial strain; Streptomycete (Streptomyces hygroscopicus) isolated from a soil sample of the Easter Island (Rapa Nui in the local language) was found to inhibit growth of a variety of fungi [START_REF] Sehgal | Rapamycin (AY-22,989), a new antifungal antibiotic. II. Fermentation, isolation and characterization[END_REF]. The antifungal principle was subsequently extracted and termed as rapamycin. It was discovered that rapamycin selectively blocked S6K1 activation in mammalian cells [START_REF] Chung | Rapamycin-FKBP specifically blocks growth-dependent activation of and signaling by the 70 kd S6 protein kinases[END_REF]. Thereafter, a tremendous amount of efforts has provided an exciting insight into a central growth regulatory signaling component named TOR, which is an evolutionary conserved element from yeast to man [START_REF] Hanrahan | Rheb activation of mTOR and S6K1 signaling[END_REF][START_REF] Dann | The amino acid sensitive TOR pathway from yeast to mammals[END_REF][START_REF] Hietakangas | Regulation of tissue growth through nutrient sensing[END_REF].

The conserved serine/threonine protein kinase TOR was originally identified in budding yeast, where gain-of-function mutations in the TOR1 and TOR2 genes were shown to confer resistance to rapamycin that inhibits yeast growth [START_REF] Heitman | Targets for cell cycle arrest by the immunosuppressant rapamycin in yeast[END_REF]. Over the last few years, TOR has been emerged as a critical regulator of the protein synthesis and cell growth in Caenorhabditis elegans, Drosophila melanogaster, yeast, plants, and mammals. Unlike yeast, which in some cases possesses two TOR genes, higher eukaryotes possess only a single TOR gene [START_REF] Lee | TOR complex 2 integrates cell movement during chemotaxis and signal relay in Dictyostelium[END_REF][START_REF] Crespo | Inhibition of target of rapamycin signaling by rapamycin in the unicellular green alga Chlamydomonas reinhardtii[END_REF]. In addition to regulating protein synthesis, mTOR (also known as FRAP, RAFT, RAPT, or SEP) signaling may also regulate transcription, cell proliferation, cytoskeletal rearrangements, ribosome biogenesis and autophagy (Fig. 1) [START_REF] Hay | Upstream and downstream of mTOR[END_REF][START_REF] Schmelzle | TOR, a central controller of cell growth[END_REF][START_REF] Martin | The expanding TOR signaling network[END_REF][START_REF] Hietakangas | Regulation of tissue growth through nutrient sensing[END_REF][START_REF] Soulard | SnapShot: mTOR signaling[END_REF].

Eukaryote TORs are large proteins (about 280 kDa) that share 40%-60% identity in their primary sequence and belong to a group of kinases known as the phosphatidylinositol kinase-related kinase (PIKK) family. PIKK family members contain a carboxy-terminal serine/threonine protein kinase domain that resembles the catalytic domain of phosphatidylinositol 3-kinases (PI3Ks) and PI4Ks. At the amino-terminal region, the FKBP12-rapamycin binding domain (FRB) is located and the FAT (FRAP, ATM, and TTRAP) and FATC domains are located towards the carboxy-terminal part (Fig. 2).

Single amino acid substitutions in FRB yield TOR proteins (such as TOR1-1 and TOR2-1) that are no longer bound and inhibited by the FKBP12-rapamycin complex. These domains are always found together in all PIKKs, suggesting that they may interact with each other. The amino-terminal portion of TOR contains tandem HEAT repeats that may form an extended super helical array with large interfaces for protein-protein interactions [START_REF] Wullschleger | TOR signaling in growth and metabolism[END_REF].

1.2.1-A central controller of cell growth

Genetic studies from multiple model organisms have demonstrated that TOR is an essential regulator of cell and tissue growth. Net tissue growth depends on the rate at which cells grow and divide. Both cell size and cell proliferation are regulated by TOR signaling [START_REF] Zhang | Regulation of cellular growth by the Drosophila target of rapamycin dTOR[END_REF][START_REF] Stanfel | The TOR pathway comes of age[END_REF][START_REF] Hietakangas | Regulation of tissue growth through nutrient sensing[END_REF].

Deletion of CeTOR in the nematode worm Caenorhabditis elegans causes developmental arrests [START_REF] Long | TOR deficiency in C. elegans causes developmental arrest and intestinal atrophy by inhibition of mRNA translation[END_REF]. TOR mutant Drosophila shows a small cell size phenotype and reduced body size, which is consistent with a role of dTOR in regulating cell growth [START_REF] Oldham | Genetic and biochemical characterization of dTOR, the Drosophila homolog of the target of rapamycin[END_REF][START_REF] Zhang | Regulation of cellular growth by the Drosophila target of rapamycin dTOR[END_REF]. It has been also shown that dTOR affects the timing of neuronal cell differentiation. Hyperactivation of dTOR signaling leads to accelerated differentiation, whereas inhibition of dTOR signaling retards differentiation [START_REF] Bateman | Temporal control of differentiation by the insulin receptor/tor pathway in Drosophila[END_REF]. Interestingly, ablation of dTOR in the fat body results in a systemic defect in larval growth [START_REF] Colombani | A nutrient sensor mechanism controls Drosophila growth[END_REF]. Thus, in addition to controlling growth of the cell in which it resides, dTOR can also influence growth of distant cells and organs during development via a humoral mechanism. Studies in yeast demonstrated that TOR regulates growth both at cellular and systemic levels. [START_REF] Loewith | Two TOR complexes, only one of which is rapamycin sensitive, have distinct roles in cell growth control[END_REF][START_REF] Friberg | The control of growth and metabolism in Caenorhabditis elegans[END_REF]. When growth conditions are favourable, TOR is active and yeast cells maintain a robust rate of ribosome biogenesis, translation initiation, and nutrient import. However, rapidly growing yeast cells treated with rapamycin, starved for nitrogen, or depleted of both TOR1 and TOR2 downregulate general protein synthesis, upregulate macroautophagy and activate several stress-responsive transcription factors [START_REF] Stanfel | The TOR pathway comes of age[END_REF][START_REF] Wullschleger | TOR signaling in growth and metabolism[END_REF]. Thus, conditional rapamycin-sensitive TOR signaling promotes anabolic processes and antagonizes catabolic processes. Many of these rapamycin-sensitive readouts of TOR are conserved in mammals. For example, TOR is also required for development in mice. Homozygous mTOR -/-embryos resemble embryos starved of amino acids and die shortly after implantation due to impaired cell proliferation in both embryonic and extra-embryonic compartments [START_REF] Gangloff | Disruption of the mouse mTOR gene leads to early postimplantation lethality and prohibits embryonic stem cell development[END_REF][START_REF] Martin | Exogenous amino acids regulate trophectoderm differentiation in the mouse blastocyst through an mTOR-dependent pathway[END_REF][START_REF] Murakami | The aperture problem in egocentric motion[END_REF]. Exposure of early mouse embryos to rapamycin also arrests cell proliferation indicating that rapamycin-sensitive mTOR function is essential during this stage of development [START_REF] Martin | Exogenous amino acids regulate trophectoderm differentiation in the mouse blastocyst through an mTOR-dependent pathway[END_REF].

mTOR exists in two distinct complexes within cells; one that contains raptor, GβL, mTOR (mammalian target of rapamycin complex 1, mTORC1), and another containing rictor, GβL, and mTOR (mammalian target of rapamycin complex 2, mTORC2). The identification of the two distinct multiprotein complexes has provided a molecular basis for understanding the complexity of mTOR signaling. mTORC1 is sensitive to rapamycin and mediates the temporal control of cell growth by regulating protein synthesis and mass accumulation through its substrates, S6K1 and 4E-BPs [START_REF] Martin | The expanding TOR signaling network[END_REF][START_REF] Hara | Raptor, a binding partner of target of rapamycin (TOR), mediates TOR action[END_REF][START_REF] Kim | GbetaL, a positive regulator of the rapamycin-sensitive pathway required for the nutrient-sensitive interaction between raptor and mTOR[END_REF][START_REF] Hay | Upstream and downstream of mTOR[END_REF][START_REF] Loewith | Two TOR complexes, only one of which is rapamycin sensitive, have distinct roles in cell growth control[END_REF]. As the cell growth in higher organisms is tightly coupled to nutrient availability, growth factors and energy status of the cell. It seems that mTORC1 integrates all three inputs to control cell growth [START_REF] Martin | The expanding TOR signaling network[END_REF] (see below). mTORC2 is rapamycin insensitive and mediates the spatial control of cell growth by regulating a Rho/GTPase signaling pathway, which impinges on the actin organization [START_REF] Sarbassov | Rictor, a novel binding partner of mTOR, defines a rapamycin-insensitive and raptor-independent pathway that regulates the cytoskeleton[END_REF]) (see below). mTORC2 is also implicated in phosphorylation of PKB on Ser 473 [START_REF] Bayascas | Regulation of Akt/PKB Ser473 phosphorylation[END_REF][START_REF] Sarbassov | Phosphorylation and regulation of Akt/PKB by the rictor-mTOR complex[END_REF]. This observation suggests the existence of feedback mechanism in which TORC2 activation leads to positive regulation of PKB signaling pathway. In summary, all the findings suggest that metazoan TOR coordinates growth and development in response to the nutritional cues.

-TORC1 is sensitive to rapamycin and regulates cell growth

As discussed above, the genetic studies in yeast demonstrated that TOR signaling regulates cell growth and overall growth of an organism. The biochemical purification of TOR1 and TOR2 from yeast led to the identification of two distinct TOR protein complexes, TORC1 and TORC2, which account for the differential sensitivity of TOR signaling to rapamycin (Fig. 3). The TORC1 contains KOG1, TCO89, LST8, and either TOR1 or TOR2 [START_REF] Loewith | Two TOR complexes, only one of which is rapamycin sensitive, have distinct roles in cell growth control[END_REF][START_REF] Reinke | TOR complex 1 includes a novel component, Tco89p (YPL180w), and cooperates with Ssd1p to maintain cellular integrity in Saccharomyces cerevisiae[END_REF][START_REF] Wullschleger | TOR signaling in growth and metabolism[END_REF].

KOG1 and LST8 have obvious mammalian sequence homologs, raptor and mLST8 also known as GβL, respectively. When mTORC1 is bound by FKBP12-rapamycin, the mTORC1 kinase activity is abrogated both in vivo and in vitro [START_REF] Hara | Raptor, a binding partner of target of rapamycin (TOR), mediates TOR action[END_REF][START_REF] Jacinto | Mammalian TOR complex 2 controls the actin cytoskeleton and is rapamycin insensitive[END_REF][START_REF] Kim | mTOR interacts with raptor to form a nutrient-sensitive complex that signals to the cell growth machinery[END_REF][START_REF] Sarbassov | Rictor, a novel binding partner of mTOR, defines a rapamycin-insensitive and raptor-independent pathway that regulates the cytoskeleton[END_REF]. The disruption of TORC1 mimics rapamycin treatment, suggesting that TORC1 mediates the rapamycin-sensitive temporal control of cell growth [START_REF] Loewith | Two TOR complexes, only one of which is rapamycin sensitive, have distinct roles in cell growth control[END_REF].

Raptor (Regulatory Associated Protein of mTOR) is a large protein (150 kDa) containing a highly conserved amino-terminal domain followed by several HEAT repeats and seven carboxy-terminal WD40 repeats [START_REF] Hara | Raptor, a binding partner of target of rapamycin (TOR), mediates TOR action[END_REF][START_REF] Kim | mTOR interacts with raptor to form a nutrient-sensitive complex that signals to the cell growth machinery[END_REF]. The interactive domains in raptor and mTOR have been difficult to map which suggests that multiple contact sites exist between these two proteins. The knockdown of KOG1/raptor in mammals, S. cerevisiae, and Drosophila phenocopies rapamycin treatment and depletion of TOR indicates that raptor functions positively in mTOR signaling [START_REF] Hara | Raptor, a binding partner of target of rapamycin (TOR), mediates TOR action[END_REF][START_REF] Jacinto | Mammalian TOR complex 2 controls the actin cytoskeleton and is rapamycin insensitive[END_REF][START_REF] Kim | mTOR interacts with raptor to form a nutrient-sensitive complex that signals to the cell growth machinery[END_REF][START_REF] Sarbassov | Rictor, a novel binding partner of mTOR, defines a rapamycin-insensitive and raptor-independent pathway that regulates the cytoskeleton[END_REF]. It has been suggested that raptor acts as an adaptor to recruit substrates to mTOR [START_REF] Choi | Two motifs in the translational repressor PHAS-I required for efficient phosphorylation by mammalian target of rapamycin and for recognition by raptor[END_REF][START_REF] Hara | Raptor, a binding partner of target of rapamycin (TOR), mediates TOR action[END_REF][START_REF] Nojima | The mammalian target of rapamycin (mTOR) partner, raptor, binds the mTOR substrates p70 S6 kinase and 4E-BP1 through their TOR signaling (TOS) motif[END_REF][START_REF] Schalm | TOS motifmediated raptor binding regulates 4E-BP1 multisite phosphorylation and function[END_REF], whereas Kim et al. described that upstream signals regulate the raptor-mTOR interaction and thereby monitor the activity of mTORC1 [START_REF] Kim | mTOR interacts with raptor to form a nutrient-sensitive complex that signals to the cell growth machinery[END_REF]. The mechanism by which FKBP12-rapamycin binding inhibits mTORC1 is not known and may indeed involve more than one mechanism [START_REF] Wullschleger | TOR signaling in growth and metabolism[END_REF]. Under some experimental conditions, FKBP12-rapamycin dissociates raptor-mTOR which suggests that FKBP12-rapamycin blocks access to the substrates [START_REF] Kim | mTOR interacts with raptor to form a nutrient-sensitive complex that signals to the cell growth machinery[END_REF]. Jacinto et al. observed that FKBP12-rapamycin inhibits mTORC1 autophosphorylation, meaning thereby that FKBP12-rapamycin inhibits intrinsic mTORC1 kinase activity and the access to an extrinsic substrate [START_REF] Jacinto | Mammalian TOR complex 2 controls the actin cytoskeleton and is rapamycin insensitive[END_REF]. Raptor also serves as a scaffold for the interaction between mTOR which is required for mTOR mediated 4E-BP1 phosphorylation and elevates mTOR kinase activity towards S6K1 [START_REF] Hara | Raptor, a binding partner of target of rapamycin (TOR), mediates TOR action[END_REF][START_REF] Schalm | Identification of a conserved motif required for mTOR signaling[END_REF]. Thus, in nutshell, TORC1 is sensitive to rapamycin and TORC1-raptor interaction leads towards the cell growth.

-TORC2 is insensitive to rapamycin

In yeast, TORC2 contains AVO1, AVO2, AVO3, BIT61, LST8, and TOR2, but not TOR1 (Fig. 3) [START_REF] Wullschleger | TOR signaling in growth and metabolism[END_REF][START_REF] Loewith | Two TOR complexes, only one of which is rapamycin sensitive, have distinct roles in cell growth control[END_REF][START_REF] Reinke | TOR complex 1 includes a novel component, Tco89p (YPL180w), and cooperates with Ssd1p to maintain cellular integrity in Saccharomyces cerevisiae[END_REF]. FKBP12rapamycin does not bind to TORC2. The disruption of TORC2 mimics TOR2 depletion which suggests that TORC2 mediates the rapamycin-insensitive spatial control of cell growth [START_REF] Loewith | Two TOR complexes, only one of which is rapamycin sensitive, have distinct roles in cell growth control[END_REF]. TORC2 is a multimeric super complex, i.e., TOR2-TOR2 interaction gives rise to a TORC2-TORC2 dimer assembly [START_REF] Wullschleger | Molecular organization of target of rapamycin complex 2[END_REF].

Yeast and mammalian TORC1 are also multimeric, as is Drosophila TOR. The elegant genetic study of Zhang et al. demonstrated that dTOR is multimeric, but they did not determine whether the multimerization corresponded to dTORC1 or dTORC2.

Multimerization may play a role in the regulation of TORC kinase activity because multimeric TORC2 appears to be more active kinase than monomeric TORC2 [START_REF] Zhang | Drosophila target of rapamycin kinase functions as a multimer[END_REF].

Mammalian TORC2 (mTORC2) contains mTOR, rictor, and mLST8, but not raptor.

Rictor (also known as mAVO3) is a large protein (about 200 kDa) but contains no obvious catalytic motifs. The knockdown of mTOR or rictor results in loss of both actin polymerization and cell spreading which is consistent with the results obtained from studies in yeast and Dictyostelium discoideum. Moreover, mTORC2 is neither bound by FKBP12-rapamycin nor FKBP12-rapamycin affects mTORC2 kinase activity in vitro, suggesting that TORC2 is insensitive to rapamycin [START_REF] Jacinto | Mammalian TOR complex 2 controls the actin cytoskeleton and is rapamycin insensitive[END_REF][START_REF] Sarbassov | Rictor, a novel binding partner of mTOR, defines a rapamycin-insensitive and raptor-independent pathway that regulates the cytoskeleton[END_REF]. mediates the rapamycin-sensitive signaling branch that couples growth to amino acid sensing. Stimuli that positively regulate TORC1 and TORC1 outputs that promote growth are depicted with black arrows. Inputs that negatively regulate TORC1 and the stress-and starvation-induced processes that TORC1 regulates negatively are depicted with red bars. TORC2 signaling is rapamycin insensitive and is required for the organization of the actin cytoskeleton (From Wullschleger et al., 2006).

-PI3K-TORC1 signaling pathway

The mTOR pathway responds to growth factors via the PI3K pathway. The activation of PI3K signaling pathway is initiated by the binding of growth factors, such as insulin and insulin growth factor-1 (IGF-1) to their tyrosine kinase receptors, G-protein coupled receptors, but also by direct interaction with oncogenic Ras [START_REF] Katso | Cellular function of phosphoinositide 3-kinases: implications for development, homeostasis, and cancer[END_REF][START_REF] Cantley | The phosphoinositide 3-kinase pathway[END_REF]. The activation of PI3K leads to production of the lipid second messenger phosphatidylinositol 3,4,5-trisphosphates (PIP3) from phosphatidylinositol 4,5diphosphates (PIP2) [START_REF] Cantley | The phosphoinositide 3-kinase pathway[END_REF]. The tumor suppressor phosphatase and tensin homologue (PTEN) reverses the action of PI3K by dephosphorylating (PIP3) and, therefore, is an essential suppressor of PI3K signaling pathway (Fig. 1).

Phosphatidylinositol 3, 4,5-trisphosphate recruits proteins containing pleckstrin homology (PH) domain to the plasma membrane, thereby coupling PI3K signals to downstream effector molecules [START_REF] Katso | Cellular function of phosphoinositide 3-kinases: implications for development, homeostasis, and cancer[END_REF]. Activation of protein kinase B (PKB), following membrane translocation is essential in mediating the effects of PI3K, not only on cell growth, but also in regulating other metabolic and anti-apoptotic effects [START_REF] Alessi | Mechanism of activation of protein kinase B by insulin and IGF-1[END_REF]. PKB is activated by phosphorylation on two key residues: Thr 308 by the phosphoinositide-dependent protein kinase (PDK1) and Ser 473 by mTORC2 (Sarbassov et al., 2005a;[START_REF] Alessi | 3-Phosphoinositide-dependent protein kinase-1 (PDK1): structural and functional homology with the Drosophila DSTPK61 kinase[END_REF]. PKB activation is thought to regulate the activation of mTORC1 [START_REF] White | Insulin signaling in health and disease[END_REF]. The activation of mTOR by PKB is indirect and involves inactivation of an inhibitor of cell growth, tumor suppressor tuberous sclerosis complex (TSC) composed of hamartin (TSC1) and tuberin (TSC2) heterodimer proteins [START_REF] Martin | The expanding TOR signaling network[END_REF]. TSC mutations are associated with an autosomal dominant genetic disorder, tuberous sclerosis (TS), a disease that is associated with cancer susceptibility, including hamartomas in various organs [START_REF] Hodges | Pathological mutations in TSC1 and TSC2 disrupt the interaction between hamartin and tuberin[END_REF]. One feature of these tumors is that they contain large cells. In Drosophila, Radimerski et al. showed that the absence of Tsc1/2 leads to the constitutive dS6K activation and the inhibition of dPKB. And also the inhibition of the dPKB could be rescued by the loss of dS6K. In contrast, the dPTEN has little effect on dS6K but negatively regulates dPKB. They also demonstrated that reduced dS6K signaling rescued the early larval lethality associated with the loss of dTSC1/2 function which suggested that the S6K pathway is a promising target for the treatment of Tuberous sclerosis complex (Radimerski et al., 2002a). Furthermore, the mutations in either dTSC1 or dTSC2 have also been identified in screens for genes that suppress cell growth [START_REF] Tapon | The Drosophila tuberous sclerosis complex gene homologs restrict cell growth and cell proliferation[END_REF]. It has been suggested

that phosphorylation of TSC2 at PKB phosphorylation sites is important for inhibition of TSC complex and cell growth [START_REF] Manning | Upregulation of renal BSC1 and TSC in prenatally programmed hypertension[END_REF][START_REF] Inoki | TSC2 is phosphorylated and inhibited by Akt and suppresses mTOR signaling[END_REF][START_REF] Potter | Akt regulates growth by directly phosphorylating Tsc2[END_REF]. However, Dong and Pan presented a report in Drosophila that is in contrast with this hypothesis [START_REF] Dong | Tsc2 is not a critical target of Akt during normal Drosophila development[END_REF]. TSC2 null flies are successfully rescued using wild-type TSC2 or PKB phosphorylation-site mutants of TSC2. These rescued mutant flies have similar sized cells compared with wild-type-rescued flies, suggesting that phosphorylation of TSC2 by PKB has no effect on function of TSC2 under conditions of normal PKB activation during Drosophila development. It is still possible that under non-physiological conditions, PKB-mediated phosphorylation of TSC2 could be relevant for regulation of cell growth (Fig. 1).

TSC2 has a GTPase-activating protein domain (GAP) and acts as a GTPase-activating protein for the small G protein Rheb [START_REF] Li | TSC2: filling the GAP in the mTOR signaling pathway[END_REF]. Rheb (Ras homolog enriched in brain) was first identified as an enhancer of cell growth that was epistatic to the TSC complex in drosophila, and places TOR and S6K downstream of Rheb [START_REF] Stocker | Rheb is an essential regulator of S6K in controlling cell growth in Drosophila[END_REF]. Rheb binds directly to the kinase domain in mTOR and activates mTOR in a GTP dependent manner (Long et al., 2005a). It has been suggested that GTP loading of Rheb, rather than mediating mTORC1 recruitment, enables Rheb to induce a conformational change in mTORC1 leading to mTORC1 activation and phosphorylation of downstream targets S6 kinase 1 (S6K1) and 4E binding protein 1 (4E-BP1) (Long et al., 2005a;[START_REF] Inoki | TSC2 is phosphorylated and inhibited by Akt and suppresses mTOR signaling[END_REF][START_REF] Jaeschke | Tuberous sclerosis complex tumor suppressor-mediated S6 kinase inhibition by phosphatidylinositide-3-OH kinase is mTOR independent[END_REF]. In drosophila, these two key substrates of TORC1 are S6 kinase and eukaryotic initiation factor 4E binding protein (4E-BP), which are the downstream effectors of PI3K-TORC1 signaling pathway, ultimately regulating the mechanisms of growth and the metabolism [START_REF] Martin | The expanding TOR signaling network[END_REF][START_REF] Kim | GbetaL, a positive regulator of the rapamycin-sensitive pathway required for the nutrient-sensitive interaction between raptor and mTOR[END_REF][START_REF] Loewith | Two TOR complexes, only one of which is rapamycin sensitive, have distinct roles in cell growth control[END_REF]Teleman et al., 2005a;[START_REF] Meyuhas | Physiological roles of ribosomal protein S6: one of its kind[END_REF].

To summarize, PI3K signaling pathway results in active PKB and PDK1. The latter can directly activate S6K (detail in section 1.3.3) whereas PKB inhibits the TSC complex thus releasing Rheb from the TSC inhibition. This, together with nutrient stimulation leads to fully active TOR that can phosphorylate S6K, 4E-BP to stimulate growth. In addition, more complexity was added to this portrait that S6K negatively modulates the effect of Insulin/insulin-like growth factors by phosphorylating IRS proteins. Thus, insulin or IGF activates a negative feedback mechanism in order to regulate its own activity (Fig. 1).

-Nutrients: a stimulus for TORC1

The nutrients fuel the growth and the development of living organisms. As already discussed in detail; the eukaryotic machinery that allows unicellular and multicellular organisms to utilize nutrients efficiently involves the highly conserved protein TOR. The regulation of TOR by nutrients is conserved from yeast to man [START_REF] Martin | The expanding TOR signaling network[END_REF][START_REF] Jacinto | TOR regulation of AGC kinases in yeast and mammals[END_REF]. One possibility is that nutrients are the primary stimulus, whereas growth factor signaling may modulate the intensity of growth in certain tissues or during specific developmental stages. Alternatively, the primary stimulus in higher organisms might be growth factor signaling, whereas the nutrient signals subsequently modify the potency of the response. The activated mTORC1 regulates the translation through the hierarchical phosphorylation event either by an activation of S6K1/2 or by an inactivation of eukaryotic translation initiation factor 4 (eIF4E) inhibitor, 4E-BP1 [START_REF] Hay | Upstream and downstream of mTOR[END_REF]. It is poorly understood how cellular responses mechanistically regulate mTORC1 signaling. It seems that the stability of the mTOR-raptor interaction strengthens during nutrient deprivation, which correlates with a decrease in in vitro kinase activity of mTORC1. Amino acids have been proposed to activate mTORC1 via inhibition of TSC1-TSC2 or through stimulation of Rheb. The stimulation of leucinestarved cells with leucine reduces the amount of raptor associated with mTORC1 and correlates with increased mTORC1 in vitro kinase activity [START_REF] Kim | mTOR interacts with raptor to form a nutrient-sensitive complex that signals to the cell growth machinery[END_REF]. This regulation requires GβL, otherwise the absence of which causes the interaction between mTOR and raptor insensitive to nutrients [START_REF] Kim | GbetaL, a positive regulator of the rapamycin-sensitive pathway required for the nutrient-sensitive interaction between raptor and mTOR[END_REF].

Gao et al. demonstrated that inactivation of TSC2 renders cells resistant to amino acid

withdrawal which suggests that the amino acids mediate signals through the TSC1-TSC2 [START_REF] Gao | Tsc tumour suppressor proteins antagonize amino-acid-TOR signaling[END_REF]. Other studies have proposed a model in which amino acids signal to mTORC1 independently of TSC2. Amino acid withdrawal still downregulates mTORC1 signaling in TSC2-deficient cells and overexpression of Rheb in Drosophila and mammalian cells allows TORC1 signaling in the absence of amino acids [START_REF] Smith | The tuberous sclerosis protein TSC2 is not required for the regulation of the mammalian target of rapamycin by amino acids and certain cellular stresses[END_REF][START_REF] Saucedo | Rheb promotes cell growth as a component of the insulin/TOR signaling network[END_REF][START_REF] Garami | Insulin activation of Rheb, a mediator of mTOR/S6K/4E-BP signaling, is inhibited by TSC1 and 2[END_REF]. Furthermore, the binding of Rheb to mTOR is regulated by amino acid sufficiency, whereas GTP charging of Rheb is independent of amino acids (Long et al., 2005b) which suggests that the amino acid signal impinges on Rheb. However, Smith et al. failed to detect amino acid regulated binding of Rheb to mTOR [START_REF] Smith | The tuberous sclerosis protein TSC2 is not required for the regulation of the mammalian target of rapamycin by amino acids and certain cellular stresses[END_REF]. TORC1 responds to nutrients despite the absence of functional Rheb and TSC orthologs in S. cerevisiae, whereas withdrawal of amino acids in mammalian cells alters the binding of raptor to mTOR [START_REF] Kim | mTOR interacts with raptor to form a nutrient-sensitive complex that signals to the cell growth machinery[END_REF].

These latter observations may suggest that amino acids are sensed by mTORC1 directly.

In addition to nutrients and growth factors, mTORC1 seems to be a major integrator of the signals that convey the overall status of the cellular environment to the protein synthetic machinery and cell growth. Clearly, the mechanism by which nutrient status is communicated to mTORC1 requires further study.

-Regulation of the translation initiation and elongation by TORC1

The importance of translation and ribosome biogenesis for cell growth has been well pointed out. The main mechanism by which TORC1 regulates tissue growth is by adjusting protein biosynthesis, which is a rate limiting process in cell growth [START_REF] Hietakangas | Regulation of tissue growth through nutrient sensing[END_REF]. Two key substrates of TORC1 are involved in the initiation of cap-dependent translation of mRNAs, ribosomal protein S6 kinase (S6K)

and eukaryotic initiation factor 4E binding protein (4E-BP) (Fig. 4) [START_REF] Tee | mTOR, translational control and human disease[END_REF]. In an un-phosphorylated state, 4E-BP binds to the eukaryotic initiation factor 4E (eIF4E). TORC1-mediated phosphorylation of 4E-BP leads to its dissociation from eIF4E, which enables the association of eIF4G and assembly of the translation preinitiation complex [START_REF] Jackson | The mechanism of eukaryotic translation initiation and principles of its regulation[END_REF][START_REF] Hietakangas | Regulation of tissue growth through nutrient sensing[END_REF][START_REF] Hayashi | The rapid activation of protein synthesis by growth hormone requires signaling through mTOR[END_REF]. TORC1 activates S6K by phosphorylating the hydrophobic motif site, which allows S6K to be further phosphorylated by PDK1. The activated S6K, in turn, phosphorylates 40S ribosomal protein S6 and eIF4B, which contributes to the association of eIF4B with the translation preinitiation complex [START_REF] Holz | mTOR and S6K1 mediate assembly of the translation preinitiation complex through dynamic protein interchange and ordered phosphorylation events[END_REF]. In addition to its role in translation initiation, S6K has been shown to phosphorylate the eukaryotic elongation factor 2 (eEF2) kinase which is associated with stimulation of peptide chain elongation [START_REF] Wang | The molecular mechanism of autophagy[END_REF]. TORC1 is also thought to regulate translation of a specific group of mRNAs containing 5"-terminal oligopyrimidine (5"-TOP) tracts in an S6K-independent manner [START_REF] Patursky-Polischuk | The TSC-mTOR pathway mediates translational activation of TOP mRNAs by insulin largely in a raptor-or rictor-independent manner[END_REF][START_REF] Pende | S6K1(-/-)/S6K2(-/-) mice exhibit perinatal lethality and rapamycin-sensitive 5'-terminal oligopyrimidine mRNA translation and reveal a mitogen-activated protein kinase-dependent S6 kinase pathway[END_REF]. These transcripts encode ribosomal proteins and translation elongation factors, thereby providing a translational control-based mechanism to regulate ribosome biogenesis.

Combining these various activities, TORC1 controls the rate of protein production in three ways: by controlling the rate of initiation of mRNA translation and the rate of elongation of the nascent polypeptides and indirectly by controlling translation of ribosomal proteins [START_REF] Hietakangas | Regulation of tissue growth through nutrient sensing[END_REF].

-Regulation of ribosome biogenesis by TORC1

Ribosomes are the molecular machines that translate mRNA into protein, and consist of two subunits. Each subunit is a complex of ribosomal RNA (rRNA) and core ribosomal proteins. The mammalian ribosome is composed of a 40S small ribosomal subunit, which is made up of 33 proteins as well as the 18S rRNA, and a 60S large ribosomal subunit, a complex of 47 proteins and the 28S, 5.8S and 5S rRNA [START_REF] Wool | Extraribosomal functions of ribosomal proteins[END_REF]. In addition to its role in regulation of translational initiation and elongation, TORC1 also regulates transcription. In yeast, several transcriptional downstream effectors of TOR have been identified [START_REF] Rohde | Nutritional control via Tor signaling in Saccharomyces cerevisiae[END_REF]. The transcriptional program downstream of TORC1 has a role in controlling the production of ribosomes and consequently the cellular biosynthetic capacity. This ensures maintenance of balance between the rate of translation and the amount of ribosomes available. There is emerging evidence from multiple experimental systems that the regulation of ribosome biogenesis is a major contributor to TOR-mediated regulation of tissue growth [START_REF] Wullschleger | TOR signaling in growth and metabolism[END_REF].

In Drosophila cells, the majority of TORC1-regulated genes are involved in various aspects of ribosome biogenesis, and many of these genes have a role in promoting cell growth [START_REF] Guertin | Functional genomics identifies TOR-regulated genes that control growth and division[END_REF]. For instance, a majority of TORC1-regulated genes appear to be targets of dMyc, the Drosophila ortholog of the oncogene c-Myc [START_REF] Teleman | Nutritional control of protein biosynthetic capacity by insulin via Myc in Drosophila[END_REF]. Inhibition of TORC1 activity leads to a rapid downregulation of dMyc expression, leading to a perturbation of ribosome biogenesis machinery which provides an explanation for the TORC1-regulated transcriptional program in Drosophila. Therefore, Myc activity is essential for TORC1-mediated tissue growth [START_REF] Hietakangas | Regulation of tissue growth through nutrient sensing[END_REF].

Myc overexpression in mice can induce the transcription of multiple genes involved in ribosome synthesis [START_REF] Kim | Identification and characterization of multiple isoforms of a mouse ribosome receptor[END_REF]. The Drosophila melanogaster genes minifly and pitchoune have been found to be required for ribosomal RNA (rRNA) processing and are also important for growth [START_REF] Giordano | minifly, a Drosophila gene required for ribosome biogenesis[END_REF][START_REF] Zaffran | A Drosophila RNA helicase gene, pitchoune, is required for cell growth and proliferation and is a potential target of d-Myc[END_REF]. Notably, pitchoune appears to be a target of Myc in flies [START_REF] Zaffran | A Drosophila RNA helicase gene, pitchoune, is required for cell growth and proliferation and is a potential target of d-Myc[END_REF]. Therefore, concisely proposed that ribosome biogenesis is mediated through TORCI and TORC1regulated target genes.

-TOR and regulation of autophagy

For the continuity of normal development of an organism, a constant supply of nutrients is required to provide energy necessary for growth and metabolism. To achieve this, eukaryotic cells have evolved a variety of mechanisms to adjust their metabolic activities in response to changes in nutrient levels. Under starvation conditions, energy demanding functions such as protein synthesis and cell cycle progression are rapidly suppressed; and patterns of gene expression, protein stability, and nutrient uptake are adjusted so that cell physiology and metabolism match the supply and quality of nutrients [START_REF] Scott | Role and regulation of starvation-induced autophagy in the Drosophila fat body[END_REF].

Central among the responses to nutrient deprivation is autophagy which is a cellular process of bulk degradation of organelles and cytoplasmic contents upon starvation and/or the process of recycling of these structures [START_REF] Mizushima | Autophagosome formation in mammalian cells[END_REF][START_REF] Wang | The molecular mechanism of autophagy[END_REF]. The process of autophagy involves the encapsulation of the material to be degraded in double membrane vesicles named "autophagosomes" which fuse with the lytic vacuole (yeast) or with lysosomes [START_REF] Stromhaug | Approaching the molecular mechanism of autophagy[END_REF]. The resulting breakdown products can be an essential source of nutrients under starvation conditions, as the mutations in many of the approximately 20 ATG genes required for autophagy in S. cerevisiae cause lethality under starvation conditions [START_REF] Klionsky | A unified nomenclature for yeast autophagy-related genes[END_REF].

Furthermore, in yeast, TOR is thought to control the autophagic machinery in part by regulating the activity of a complex containing the serine-threonine kinase Atg1 and by regulating expression of ATG8 [START_REF] Kamada | Tor-mediated induction of autophagy via an Apg1 protein kinase complex[END_REF][START_REF] Kirisako | Formation process of autophagosome is traced with Apg8/Aut7p in yeast[END_REF]. Autophagy also plays an important housekeeping role in turnover of damaged organelles, in cellular remodelling during development, and in some types of programmed cell death [START_REF] Levine | Development by self-digestion: molecular mechanisms and biological functions of autophagy[END_REF]. Many of the cellular processes to changes in nutrient levels, including autophagy are regulated by TOR (Fig. 5). Inactivation of TOR by mutation or by treatment with the drug rapamycin induces autophagy despite the presence of ample nutrients indicates that TOR acts to suppress autophagy under non-starvation conditions [START_REF] Jacinto | Tor signaling in bugs, brain and brawn[END_REF][START_REF] Noda | Tor, a phosphatidylinositol kinase homologue, controls autophagy in yeast[END_REF][START_REF] Scott | Role and regulation of starvation-induced autophagy in the Drosophila fat body[END_REF]. It has been also

shown in mammalian system that the mTOR is likely to be involved in the regulation of autophagy [START_REF] Dennis | Target of rapamycin (TOR): balancing the opposing forces of protein synthesis and degradation[END_REF]. In higher eukaryotes, starvation-induced autophagy is also suppressed by components of the Insulin/PI3K pathway upstream of the TOR [START_REF] Melendez | Autophagy genes are essential for dauer development and life-span extension in C. elegans[END_REF].

Apart from their role in suppressing autophagy, the insulin/PI3K and TOR pathways are well established as potent oncogenic activators of cell growth [START_REF] Kozma | Regulation of cell size in growth, development and human disease: PI3K, PKB and S6K[END_REF]. As the growth rate of a cell reflects the balance between anabolic processes (protein synthesis) and catabolic processes (protein degradation), therefore, this raised the possibility that TOR may regulate growth in part through suppression of autophagy (Fig. 5). In Drosophila, in late larval stages and during metamorphosis, cytoplasmic contents within the fat body are degraded by autophagy [START_REF] Rusten | Programmed autophagy in the Drosophila fat body is induced by ecdysone through regulation of the PI3K pathway[END_REF]. Scott et al.

provided a genetic evidence that under conditioning of low TOR signaling, autophagy functions primarily to promote normal cell functioning and survival, rather than to suppress cell growth [START_REF] Scott | Role and regulation of starvation-induced autophagy in the Drosophila fat body[END_REF]. Thus in a variety of signaling and developmental contexts, TOR acts as a negative regulator of autophagy and promotes protein synthesis and growth as long as there is a provision of nutrients [START_REF] Schmelzle | TOR, a central controller of cell growth[END_REF]. These findings also suggest that autophagy is a default mechanism that requires permanent active repression by a TOR dependent mechanism if the cell is to grow in the absence of nutrients.

Figure 5 -Interrelations between TOR signaling, autophagy and cell growth

TORC1 signaling promotes cell growth through its effects both on increasing cell proliferation and increasing cell size, and also through its inhibitory effects on autophagy. TORC1 increases protein synthesis and cell size through intermediates such as 4E-BP and S6K. In contrast, autophagy is believed to inhibit cell growth at least in part by promoting protein organelle turnover. Note that the presence of growth factors and nutrients are all required for the full activation of TORC1 signaling, whereas the absence of any of these factors is sufficient to induce autophagy. (drawn from [START_REF] Wang | Autophagy in cellular growth control[END_REF].

-Metabolic functions of TOR signaling

The target of rapamycin (TOR) signaling pathway responds to cellular levels of amino acids and ATP through its upstream effectors, the tuberous sclerosis complex (TSC1 & TSC2) and Rheb GTPase. Insulin/TOR signaling pathway directs critical changes in cellular physiology that link growth, translation, and autophagy to the nutrient status of the animal [START_REF] Edgar | How flies get their size: genetics meets physiology[END_REF][START_REF] Hay | Upstream and downstream of mTOR[END_REF]. For example, TOR kinase is active in the presence of sufficient nutrients, phosphorylating S6 kinase (S6K), regulating protein synthesis and hence facilitates growth [START_REF] Hietakangas | Regulation of tissue growth through nutrient sensing[END_REF]. In addition, Akt mediated phosphorylation of a key transcriptional effector of insulin signaling, dFOXO, renders it inactive by restricting it to the cytoplasm [START_REF] Baker | Diabetic larvae and obese flies-emerging studies of metabolism in Drosophila[END_REF]. In contrast, TOR activity is reduced under starvation conditions, leading to a decreased translational capacity. The non-phosphorylated dFOXO translocates into the nucleus, triggering a transcriptional program that includes the upregulation of 4E-BP, which controls lipid mobilization (Teleman et al., 2005a).

Furthermore, a hypomorphic dTOR allele has been described with reduced fat body lipid levels and increased β-hydroxybutyrate levels, indicating an increased conversion of lipids into ketone bodies and reduced glucose levels. These findings provide a new genetic system to better characterize the effects of TOR signaling on the energy metabolism [START_REF] Luong | Activated FOXO-mediated insulin resistance is blocked by reduction of TOR activity[END_REF].

Although amino acid levels are known to modulate TOR activity, the mechanisms that underlie this response remain poorly understood. Genetic characterization of the Slimfast amino acid transporter has provided some initial clues into this pathway, indicating that it functions as a nutrient sensor in the larval fat body and controlling a systemic response which links amino acid levels with organismal growth. The observation that fat body specific inactivation of either slimfast or dTOR leads to similar phenotypes supports the notion that Slimfast can signal through dTOR in the fat body to globally regulate growth and metabolism in response to amino acid levels. This fat body amino acid sensor pathway can override insulin signaling in peripheral tissues through inhibition of PI3K

activity, apparently through one or more unidentified factors that emanate from the fat body [START_REF] Colombani | A nutrient sensor mechanism controls Drosophila growth[END_REF].

-Drosophila as a system for studies of lipid metabolism

The mammalian genome is thought to have been duplicated two times which in some cases makes phenotypic characterization of a specific gene deficiency difficult [START_REF] Miklos | The role of the genome project in determining gene function: insights from model organisms[END_REF]. Therefore, in some instances it is more attractive to study gene function in less complex organisms, such as Drosophila melanogaster, in which individual genes are usually represented in a single copy. Additionally, the mammalian signaling pathways are largely conserved in Drosophila which offers a powerful genetic system and thereby, provides a unique opportunity to uncover critical new insights into the central regulatory pathways that are conserved through evolution. Therefore, the past few years have seen a shift in direction, in which Drosophila is being increasingly exploited to understand the regulatory mechanisms of metabolism [START_REF] Beller | COPI complex is a regulator of lipid homeostasis[END_REF].

Furthermore, the drosophila genetic system has been implicated also in the better understanding and the treatment of human disease risk factors, such as diabetes and obesity [START_REF] Baker | Diabetic larvae and obese flies-emerging studies of metabolism in Drosophila[END_REF]. Indeed, even the relatively few Drosophila data in literature described, have changed our understanding of vertebrate metabolic control. For example, contrary to studies in mammalian cell culture, 4E-BP regulates organismal lipid homeostasis rather than growth (Teleman et al., 2005a). Similarly, the discovery of evolutionarily conserved essential regulators of lipid metabolism in Drosophila, such as melted and adipose, provide new directions for understanding the control of these pathways in humans and also raise the possibility that mutations in these genes may be risk factors for human metabolic disorders (Teleman et al., 2005b). Therefore, growing studies and research work have been emerged in which Drosophila has been constantly embarked as a model system for the study of lipid metabolism [START_REF] Baker | Diabetic larvae and obese flies-emerging studies of metabolism in Drosophila[END_REF][START_REF] Beller | COPI complex is a regulator of lipid homeostasis[END_REF][START_REF] Farese | Lipid droplets finally get a little R-E-S-P-E-C-T[END_REF][START_REF] Diangelo | Regulation of fat cell mass by insulin in Drosophila melanogaster[END_REF][START_REF] Beller | PERILIPIN-Dependent Control of Lipid Droplet Structure and Fat Storage in Drosophila[END_REF].

Lipid metabolism is essential for growth and generates much of the energy needed for normal developmental process as well as during periods of food deprivation. The larvae of Drosophila have been used extensively to study for regulation of growth and lipid metabolism [START_REF] Colombani | A nutrient sensor mechanism controls Drosophila growth[END_REF]. The bulk of the Drosophila larva is made up of endoreplicating tissues (ERTs) that are histolyzed during the pupal phase to support metamorphosis. During metamorphosis, mitotic tissues known as imaginal discs are recognized into the adult fly. In insects and particularly in Drosophila, lipids are stored in a specialized tissue that resembles the adipose tissue of mammals, the fat body. The fat body is an ERT, which is particularly sensitive to nutrients and alters its morphology dramatically in response to amino acid starvation [START_REF] Britton | Environmental control of the cell cycle in Drosophila: nutrition activates mitotic and endoreplicative cells by distinct mechanisms[END_REF] [START_REF] Colombani | A nutrient sensor mechanism controls Drosophila growth[END_REF]. Starvation induced fat release is accompanied by increased lipolysis, at least in part associated with upregulation of Brummer, an ATGL (Adipose Triglyceride Lipase) related lipase localized to lipid droplets. Fat mobilization is also influenced by LSD2

(lipid storage droplet-2), a lipid droplet protein related to a mammalian negative regulator of TAG (Triglycerides) hydrolysis called PERILIPIN. In addition to its involvement in lipid storage and release, the fat body produces a humoral signal regulating larval tissue growth in response to food availability. In addition, Drosophila maintains appropriate circulating sugar levels, compensating for changing environmental conditions and storing excess energy in the forms of glycogen and lipid. These reserves are mobilized during periods of energy need, such as nutrient depletion [START_REF] Rusten | Programmed autophagy in the Drosophila fat body is induced by ecdysone through regulation of the PI3K pathway[END_REF][START_REF] Scott | Role and regulation of starvation-induced autophagy in the Drosophila fat body[END_REF]. Many of the analogous organ systems that control nutrient uptake, storage, and metabolism in humans are present in the fruit fly. Digestion and nutrient absorption occur in the Drosophila midgut, the functional equivalent of the stomach and intestine. The fat body acts like the mammalian liver and white adipose tissue, metabolizing nutrients and storing large reserves as stores. Hence, the central pathways of intermediary metabolism and regulators of homeostasis are present in the fly which demonstrates that most of the essential metabolic functions have been conserved through evolution [START_REF] Baker | Diabetic larvae and obese flies-emerging studies of metabolism in Drosophila[END_REF]. In nutshell, based on functional analogy and the powerful genetic system, Drosophila could serve as a good model for metabolic studies.

-Structure of a lipid droplet

The earliest descriptions of lipid droplets date to the 19 th century. The identification through light microscopy and the speculation of their origin gave an importance to lipid droplets and in the meantime a respectable name was given to these organelles: liposome [START_REF] Farese | Lipid droplets finally get a little R-E-S-P-E-C-T[END_REF]. However, in the late 1960s, the organelles were called by many names, including lipid droplets, lipid bodies, fat bodies fat droplets, and adiposmes. In plants, they are often called oil bodies. As the field continuously rapidly evolved, it seemed to be settling on the name "lipid droplets" (LDs) [START_REF] Martin | Lipid droplets: a unified view of a dynamic organelle[END_REF][START_REF] Farese | Lipid droplets finally get a little R-E-S-P-E-C-T[END_REF].

These universal organelles are found in most eukaryotic cells and range greatly in size (< 1-100 μm); the diameter varies tremendously depending on the cell type [START_REF] Murphy | The biogenesis and functions of lipid bodies in animals, plants and microorganisms[END_REF][START_REF] Farese | Lipid droplets finally get a little R-E-S-P-E-C-T[END_REF]. However their structure appears to be fairly consistent and each consists of a phospholipid monolayer that surrounds a core of neutral lipids, such as sterol esters or triacylglycerols (Fig. 6). Numerous proteins, many of which play functional roles in LD biology, decorate their surfaces [START_REF] Farese | Lipid droplets finally get a little R-E-S-P-E-C-T[END_REF][START_REF] Goodman | The gregarious lipid droplet[END_REF][START_REF] Beller | Characterization of the Drosophila lipid droplet subproteome[END_REF][START_REF] Cermelli | The lipid-droplet proteome reveals that droplets are a protein-storage depot[END_REF]. The mechanism of LD formation is uncertain; the prevailing model posits that LDs form at the ER, where the enzymes that catalyze neutral lipid synthesis are located. Eventually, the growing oil droplet buds from the ER membrane, forming a detached cytosolic LD, or stays in contact with the ER membrane and forms a specialized LD and ER domain [START_REF] Farese | Lipid droplets finally get a little R-E-S-P-E-C-T[END_REF][START_REF] Martin | Lipid droplets: a unified view of a dynamic organelle[END_REF]. The ability to isolate LDs to high purity, together with the modern techniques of proteomics, has allowed an elucidation of the LD proteome under different experimental conditions. This has yielded maps of the "housekeeping" proteins of LDs, as well as unexpected insights into their possible interactions with other organelles. Among the several proteins that have been identified on LDs, are intimately linked to vesicular transport, membrane fusion and cytoskeletal motility [START_REF] Beller | Characterization of the Drosophila lipid droplet subproteome[END_REF][START_REF] Cermelli | The lipid-droplet proteome reveals that droplets are a protein-storage depot[END_REF][START_REF] Martin | Lipid droplets: a unified view of a dynamic organelle[END_REF]. Some of the best-understood LD components are members of the PAT-domain (Perilipin, ADRP and TIP47-related protein domain) family of proteins [START_REF] Farese | Lipid droplets finally get a little R-E-S-P-E-C-T[END_REF][START_REF] Tansey | The central role of perilipin a in lipid metabolism and adipocyte lipolysis[END_REF]. The term PAT domain refers to a region of sequence similarity that is present in the founding members of this family: perilipin, adipose differentiation-related protein (ADRP; also called adipophilin) and tail-interacting protein of 47 kDa (TIP47). PAT domain proteins including LSD2 (lipid storage droplet-2) are evolutionarily conserved with homologs in both Dictyostelium discoideum and D. melanogaster [START_REF] Miura | Functional conservation for lipid storage droplet association among Perilipin, ADRP, and TIP47 (PAT)-related proteins in mammals, Drosophila, and Dictyostelium[END_REF]. Adipophilin is a ubiquitously expressed protein in all mammalian cell types and found only in lipid droplets [START_REF] Brasaemle | Adipose differentiation-related protein is an ubiquitously expressed lipid storage droplet-associated protein[END_REF][START_REF] Heid | Adipophilin is a specific marker of lipid accumulation in diverse cell types and diseases[END_REF]. Perilipin, a structurally related protein, associates with lipid droplets in adipocytes and steroidogenic cells [START_REF] Greenberg | Perilipin, a major hormonally regulated adipocyte-specific phosphoprotein associated with the periphery of lipid storage droplets[END_REF]. In these cells, it restricts the access of hormone-sensitive lipase and other cytosolic lipases to the lipid droplet under basal conditions and facilitates lipase access to the droplet under lipolytically stimulating conditions [START_REF] Brasaemle | The lipolytic stimulation of 3T3-L1 adipocytes promotes the translocation of hormonesensitive lipase to the surfaces of lipid storage droplets[END_REF]. The first genetic study of an essential enzyme in lipolysis, the ATGL (Adipose Triglyceride Lipase) homolog Brummer, foreshadowed genetic studies of its vertebrate counterpart in mice [START_REF] Gronke | Brummer lipase is an evolutionary conserved fat storage regulator in Drosophila[END_REF][START_REF] Haemmerle | Defective lipolysis and altered energy metabolism in mice lacking adipose triglyceride lipase[END_REF].

Furthermore, Rusten et al. investigated the starvation induced autophagy at deferent developmental stages in the fat body of Drosophila. The larvae fed on amino aciddeficient food display a quick starvation-induced response in the fat body, leading to the aggregation of the storage vesicles, the lipid droplets (LDs) [START_REF] Rusten | Programmed autophagy in the Drosophila fat body is induced by ecdysone through regulation of the PI3K pathway[END_REF].

Therefore, LDs are being used for the study of metabolism studies as a quick tool. The phenotype of these vesicles can be traced out easily and, hence, has been retained to study a gene function in Drosophila [START_REF] Britton | Drosophila's insulin/PI3-kinase pathway coordinates cellular metabolism with nutritional conditions[END_REF][START_REF] Colombani | A nutrient sensor mechanism controls Drosophila growth[END_REF][START_REF] Zhang | Regulation of cellular growth by the Drosophila target of rapamycin dTOR[END_REF][START_REF] Beller | COPI complex is a regulator of lipid homeostasis[END_REF][START_REF] Farese | Lipid droplets finally get a little R-E-S-P-E-C-T[END_REF][START_REF] Diangelo | Regulation of fat cell mass by insulin in Drosophila melanogaster[END_REF][START_REF] Beller | PERILIPIN-Dependent Control of Lipid Droplet Structure and Fat Storage in Drosophila[END_REF].

-S6 Kinase

-Ribosomal protein S6 phosphorylation

As described earlier, higher eukaryotic ribosomes consist of two subunits designated as 40S (small) and 60S (large) subunits. The 40S subunit comprises a single molecule of RNA, termed 18S rRNA, and 33 proteins; by contrast, the 60S subunit has three RNA molecules, termed 5S, 5.8S and 28S rRNA, and 47 proteins [START_REF] Wool | Extraribosomal functions of ribosomal proteins[END_REF]. It was discovered in 1970s that the 30 kDa ribosomal protein S6 became phosophorylated in the liver of partially hepatectomized rats [START_REF] Gressner | The phosphorylation of liver ribosomal proteins in vivo. Evidence that only a single small subunit protein (S6) is phosphorylated[END_REF]. Following partial hepatectomy, the remaining hepatocytes re-enter the cell cycle and proliferate to regenerate the liver. In the same organ, in vivo S6 phosphorylation was induced after refeeding starved rats. Starvation induces hepatic autophagy and atrophy, which was reversed upon refeeding [START_REF] Kozma | A stimulated S6 kinase from rat liver: identity with the mitogen activated S6 kinase of 3T3 cells[END_REF]. In addition, a variety of hormones and growth factors have been reported to induce S6 phosphorylation. Thus S6 phosphorylation appears to correlate with increased rates of protein synthesis and hence cell growth. It has been also described that protein phosphorylation can modify enzymatic activity or create a binding site for other proteins, so it has been speculated that S6 phosphorylation may serve to increase translation rates when cells are to grow [START_REF] Stewart | Mitogenesis and protein synthesis: a role for ribosomal protein S6 phosphorylation?[END_REF]. In support of this notion, S6 is positioned in the small head region of the 40S ribosomal subunit at the interface with the 60S subunit. There, S6 is thought to contact mRNA and the 28S rRNA directly [START_REF] Nygard | Translational dynamics. Interactions between the translational factors, tRNA and ribosomes during eukaryotic protein synthesis[END_REF].

Subsequent analysis revealed that S6 became phosphorylated at multiple sites in an ordered fashion. The mammalian S6 phosphorylation sites were identified as Ser 235 , Ser 236 , Ser 240 , Ser 244 and Ser 247 all located in the carboxyl-terminal region [START_REF] Bandi | Identification of 40 S ribosomal protein S6 phosphorylation sites in Swiss mouse 3T3 fibroblasts stimulated with serum[END_REF][START_REF] Volarevic | Role of S6 phosphorylation and S6 kinase in cell growth[END_REF]. The ribosomal protein S6, including its phosphorylation sites, is highly conserved among vertebrates [START_REF] Meyuhas | Physiological roles of ribosomal protein S6: one of its kind[END_REF]. In fact, five phosphorylation sites in the carboxyl-terminus of Drosophila S6 (dS6) have been identified [START_REF] Volarevic | Role of S6 phosphorylation and S6 kinase in cell growth[END_REF][START_REF] Radimerski | Identification of insulin-induced sites of ribosomal protein S6 phosphorylation in Drosophila melanogaster[END_REF]. Furthermore, the S. cerevisiae and S. pombe orthologue, ribosomal, protein S10, also contains two phosphorylation sites [START_REF] Johnson | Phosphorylation of the Saccharomyces cerevisiae equivalent of ribosomal protein S6 has no detectable effect on growth[END_REF]. Thus ribosomal protein phosphorylation appears to be conserved from yeast to man (Fig. 7), suggesting that it has been implicated for its function in translational control. Of all ribosomal proteins, it is the ribosomal protein S6 (RPS6) that has attracted much attention, since it is the first, and was for many years the only one that has been shown to undergo inducible phosphorylation. It is only recently that the role of RPS6 and its post-translational modification has been started being disclosed by genetic targeting of the RPS6 gene and of the respective kinases [START_REF] Meyuhas | Physiological roles of ribosomal protein S6: one of its kind[END_REF]. However it must be emphasized that direct evidence for regulation of protein synthesis by S6 phosphorylation needs further investigations.

-Identification of the S6K1

Keeping in view that ribosomal protein S6 phosphorylation correlates with increased translation and growth, efforts started focusing on the identification of in vivo S6 kinase in order to understand the molecular signaling pathway that communicate extra-cellular stimuli to the ribosome [START_REF] Meyuhas | Physiological roles of ribosomal protein S6: one of its kind[END_REF]. Although a number of kinases were initially implicated in S6 phosphorylation based on in vitro activities; it has been generally accepted that the S6K family of kinases mediates this response [START_REF] Volarevic | Role of S6 phosphorylation and S6 kinase in cell growth[END_REF]. S6K (S6 kinase) is a protein kinase belonging to the AGC (protein kinase A/ protein kinase G/ protein kinase C) kinase family and a key regulator of protein synthesis [START_REF] Jacinto | TOR regulation of AGC kinases in yeast and mammals[END_REF]. Characterization of an S6 kinase at a molecular level was first described in Xenopus oocytes wherein a dominant form of S6 kinase detected after mitogen stimulation, had been purified as a 90-kDa polypeptide [START_REF] Erikson | A protein kinase from Xenopus eggs specific for ribosomal protein S6[END_REF], later termed as p90 ribosomal protein S6 kinase (p 90RSK ). Purification of the avian and mammalian RPS6 recovered 65-70 kDa polypeptides that are currently referred to as S6K [START_REF] Meyuhas | Physiological roles of ribosomal protein S6: one of its kind[END_REF].

Mammalian cells contain two forms of S6K, S6K1 & S6K2 (Fig. 8), which is encoded by two different genes and shares a very high level of overall sequence homology. S6K1

has cytosolic and nuclear isoforms (p 70S6K1 and p 85S6K1 , respectively), whereas both S6K2 isoforms (p 54S6K2 & p 56S6K2 ) are primarily nuclear, and partly associated with the centrosome [START_REF] Rossi | Identification of S6K2 as a centrosome-located kinase[END_REF][START_REF] Meyuhas | Physiological roles of ribosomal protein S6: one of its kind[END_REF]). Analysis of RPS6 phosphorylation in mouse cells deficient in either S6K1 or S6K2 suggested that both are required for full S6 phosphorylation, with the predominance of S6K2 [START_REF] Pende | S6K1(-/-)/S6K2(-/-) mice exhibit perinatal lethality and rapamycin-sensitive 5'-terminal oligopyrimidine mRNA translation and reveal a mitogen-activated protein kinase-dependent S6 kinase pathway[END_REF]. Notably, the phosphorylation of the evolutionary conserved sites of Drosophila RPS6 has been carried out by dS6K that is encoded by a single gene (Fig. 8) [START_REF] Watson | A Drosophila gene structurally and functionally homologous to the mammalian 70-kDa s6 kinase gene[END_REF].

The S6 kinase signaling pathway, which in many cell types, is triggered by the recruitment of phosphatidylinositide-3OH kinase (PI3K) to the activated receptor and the production of phosphatidylinositol-3,4,5-trisphosphate (PIP3). The mechanism by which kinase activation is presumed to take place has been acceded by the dissection of the primary structure of S6Kl into domains and to the identification of specific regulatory phosphorylation sites [START_REF] Volarevic | Proliferation, but not growth, blocked by conditional deletion of 40S ribosomal protein S6[END_REF]. There are five distinct domains that cooperate to bring about S6 kinase activation. The first is an acidic domain that extends from the short amino-terminus to the beginning of the catalytic domain and confers rapamycin sensitivity on the kinase [START_REF] Dennis | The principal rapamycin-sensitive p70(s6k) phosphorylation sites, T-229 and T-389, are differentially regulated by rapamycin-insensitive kinase kinases[END_REF]. The second is the catalytic domain, which contains the mitogen-induced phosphorylation site T 229 , residing within the activation loop. The catalytic domain, and sequence immediately after the amino terminal to the T 229 phosphorylation site are highly conserved in the protein A, protein G, and protein C (AGC) family of protein kinases. The third domain is the linker domain, which connects the catalytic domain to the autoinhibitory domain [START_REF] Pullen | The modular phosphorylation and activation of p70s6k[END_REF]. The linker domain is also highly conserved in the AGC family of serine threonine kinases and contains two phosphorylation sites, S 371 and T 389 , which are essential for S6Kl activation [START_REF] Pearson | The principal target of rapamycin-induced p70s6k inactivation is a novel phosphorylation site within a conserved hydrophobic domain[END_REF]. Immediately, downstream of the auto-inhibitory domain is the amino terminus, which has been implicated in the interaction of the kinase with other proteins [START_REF] Burnett | Neurabin is a synaptic protein linking p70 S6 kinase and the neuronal cytoskeleton[END_REF]. [START_REF] Beller | Characterization of the Drosophila lipid droplet subproteome[END_REF].

Figure 7 -Comparison of carboxy-terminal domain of ribosomal protein S6

Carboxy-terminal domain of ribosomal protein S6 along with the phosphorylation sites in different species are shown (From Meyuhas, 2008;[START_REF] Volarevic | Role of S6 phosphorylation and S6 kinase in cell growth[END_REF].

-Activation of S6K

The basic mechanism of the kinase activation was preceded by the identification of the signaling components that mediate S6K1 activation. Activation of S6K1 requires phosphorylation of four S/T-P sites, which reside in the auto-inhibitory domain [START_REF] Dennis | Phosphorylation sites in the autoinhibitory domain participate in p70(s6k) activation loop phosphorylation[END_REF]. This event, in combination with phosphorylation of S 371 in the linker domain, facilitates T 389 phosphorylation, which also resides in the linker domain, and provides a docking site for the phosphoinositide-dependent protein kinase, (PDK1) [START_REF] Biondi | The PIFbinding pocket in PDK1 is essential for activation of S6K and SGK, but not PKB[END_REF]. PDK1 docks on T 389 and phosphorylates T 229 in the activation loop of the kinase, leading to kinase activation (Fig. 9). The critical event then in activating S6K is phosphorylation of T 389 [START_REF] Dennis | Phosphorylation sites in the autoinhibitory domain participate in p70(s6k) activation loop phosphorylation[END_REF]. A number of kinases have been suggested as potential T 389 kinases, including protein kinase B and PDK1 [START_REF] Burgering | Protein kinase B (c-Akt) in phosphatidylinositol-3-OH kinase signal transduction[END_REF][START_REF] Balendran | Evidence that 3-phosphoinositide-dependent protein kinase-1 mediates phosphorylation of p70 S6 kinase in vivo at Thr-412 as well as Thr-252[END_REF]. However, it has been found that the mammalian target of rapamycin, mTOR, is a potent T 389 kinase [START_REF] Dennis | Phosphorylation sites in the autoinhibitory domain participate in p70(s6k) activation loop phosphorylation[END_REF]. As already discussed in detail, mTOR is a member of the PI3K related family of protein kinases, acts as an amino acid and energy effector. If either amino acids, especially branch-chain amino acids, or ATP levels drop, mTOR activity decreases leading to inhibition of S6K1 [START_REF] Dennis | Mammalian TOR: a homeostatic ATP sensor[END_REF] The phosphorylation sites and the domain structure are well conserved in drosophila S6 kinase [START_REF] Stewart | The Drosophila p70s6k homolog exhibits conserved regulatory elements and rapamycin sensitivity[END_REF][START_REF] Watson | A Drosophila gene structurally and functionally homologous to the mammalian 70-kDa s6 kinase gene[END_REF].

The protein phosphatase 2A (PP2A), a conserved component of the insulin pathway in flies and in mammals, dephosphorylates S6K. PP2A knockout flies showed elevated S6K phosphorylation, thereby making an activation of S6K. In addition, the B′ subunit of protein phosphatase 2A (PP2A-B′) interacts with S6K [START_REF] Hahn | PP2A regulatory subunit PP2A-B' counteracts S6K phosphorylation[END_REF]. Downstream of S6K, there are alleles of the kinase which are either rapamycin resistant or dominant interfering has provided evidence that the kinase regulates the expression of a family of mRNAs at the translational level [START_REF] Fumagalli | S6 phosphorylation and signal transduction[END_REF]. This family is small, may be representing one to two hundred gene products. However, they can account for up to 20-30% of the mRNA in the cell and they largely encode for the components of translational apparatus, particularly of ribosomal proteins [START_REF] Meyuhas | Physiological roles of ribosomal protein S6: one of its kind[END_REF].

-An overview of S6K function 1.3.4.1 -S6K1 deletion in the mouse

The physiological importance of S6K1 in mammalian cells has been largely addressed in tissue culture model systems. However, in order to obtain a corroborative in vivo data in animal to support and elicit a role of S6K1 in cell growth, initial studies employed microinjection of neutralizing antibodies against S6K1 in rat embryonic fibroblast cells. These studies suggested that the kinase was essential for G1 phase progression and entry into the S-phase [START_REF] Lane | Functional expression of rat alpha 1 Na,K-ATPase containing substitutions for cysteines 454, 458, 459, 513 and 551[END_REF]. Contrary to this, the disruption of S6K1 in the embryo stem (ES) cells showed a reduction in the proliferation rate as compared to the parental cells.

This study suggested that S6K1 had a positive effect on proliferation but not essential.

The translation rates of 5"TOP mRNAs in S6K1 null ES cells, which lack S6K phosphorylation, did not change significantly in the presence or absence of serum, and the percentage of mRNAs recruited in the polysome fraction was intermediate compared to the control cells. Furthermore, the overall protein synthesis was repressed to similar extents by rapamycin treatment in both S6K1 null and wild type ES cells, while 5"TOP mRNA translation was only mildly affected in the former cells. Thus, in the absence of S6K1, the translational control of 5"TOP mRNAs was deregulated possibly by a compensatory mechanism that could involve the down-regulation of a putative repressor protein that normally binds 5"TOP mRNA [START_REF] Kawasome | Targeted disruption of p70(s6k) defines its role in protein synthesis and rapamycin sensitivity[END_REF].

In 1998, Shima et al. reported S6K1 knockout mice with small size phenotype which was particularly pronounced during embryogenesis [START_REF] Shima | Disruption of the p70(s6k)/p85(s6k) gene reveals a small mouse phenotype and a new functional S6 kinase[END_REF]. The proliferation of S6K1 null mouse embryonic fibroblast (MEFs) in culture was intact as well as the regulation of 5"TOP mRNA translation which is in contradiction to the findings of Kawasome et al. It would be noteworthy that following starvation and refeeding, the induction of S6 phosphorylation in the liver was normal. This suggested that a distinct rapamycin sensitive kinase is the physiological S6 kinase, or possibly that it functions in a redundant manner in the absence of S6K1. The S6K1 homolog termed as S6K2 was upregulated in all the tissues examined of the S6K1 null mice and it was revealed that S6K2 was capable to phosphorylate the ribosomal protein S6. The catalytic and regulatory domains as well as the regulatory phosphorylation sites are conserved between S6K1 and S6K2 [START_REF] Shima | Disruption of the p70(s6k)/p85(s6k) gene reveals a small mouse phenotype and a new functional S6 kinase[END_REF]. However, S6K2 contains a proline rich region (Fig. 8) in the carboxyl-terminal tail and is less sensitive to inhibition by rapamycin compared to S6K1 [START_REF] Gout | Molecular cloning and characterization of a novel p70 S6 kinase, p70 S6 kinase beta containing a proline-rich region[END_REF][START_REF] Saitoh | Cloning and characterization of p70(S6K beta) defines a novel family of p70 S6 kinases[END_REF]. The presence and upregulation of S6K2 may obscure and compensate some functions of S6K1 and also the characterization of the S6K1 and S6K2 double knockout mouse is anticipated. In spite of the apparent normal S6 phosphorylation and the regulation of 5"TOP mRNA translation in mice lacking S6K1, the small mouse phenotype indicates that S6K2 cannot compensate for all the functions of S6K1.

Hence, S6K is an evolutionary conserved determinant of growth through increased protein biosynthesis. The downstream target of S6K, the 40S ribosomal protein S6 has been thought to regulate the translation of 5"TOP mRNAs which encode proteins important for translational machinery. However, many reports questioned this model since S6 phosphorylation and translation of 5"TOP mRNAs are not correlated [START_REF] Pende | S6K1(-/-)/S6K2(-/-) mice exhibit perinatal lethality and rapamycin-sensitive 5'-terminal oligopyrimidine mRNA translation and reveal a mitogen-activated protein kinase-dependent S6 kinase pathway[END_REF]. Furthermore, S6 phosphorylation is not affected in S6K1-/-mice, even though these mice have a reduction in size compared to wild type. In addition, the protein S6 was also phosphorylated in an S6K1-/-and S6K2-/-double mutant mouse [START_REF] Pende | S6K1(-/-)/S6K2(-/-) mice exhibit perinatal lethality and rapamycin-sensitive 5'-terminal oligopyrimidine mRNA translation and reveal a mitogen-activated protein kinase-dependent S6 kinase pathway[END_REF]. This suggested that S6 might be phosphorylated by other kinases. Moreover, in C.

elegans, rsks-1 (S6K homolog) could possibly affect growth through regulation of ribosome biosynthesis [START_REF] Friberg | The control of growth and metabolism in Caenorhabditis elegans[END_REF]. Future studies will shed light on the regulation of ribosome biosynthesis and protein levels and also the biochemical function of S6K in more detail for the overall scenario of the regulation of cell growth.

-dS6K and the regulation of growth

The Drosophila genome contains a single S6K gene, termed as dS6K, which is highly homologous to mammalian S6K1 and S6K2. The dS6K protein has an overall identity of 57% with either S6Kl or S6K2 and 78% identity in the catalytic domain. The organization of functional domains and key phosphorylation sites is also conserved between the two enzymes. In addition, it has been proposed that upstream elements and downstream effectors of S6K are conserved in mammals and flies [START_REF] Stewart | The Drosophila p70s6k homolog exhibits conserved regulatory elements and rapamycin sensitivity[END_REF][START_REF] Watson | A Drosophila gene structurally and functionally homologous to the mammalian 70-kDa s6 kinase gene[END_REF]. The two S6K homolog which are actually expressed in mammals could overlap in their individual function [START_REF] Shima | Disruption of the p70(s6k)/p85(s6k) gene reveals a small mouse phenotype and a new functional S6 kinase[END_REF]. This functionality, hence, represents an enormous challenge to unravel the role of S6K signaling pathway in a simpler genetically amenable model organism, such as Drosophila. In Drosophila, the dS6K activity is sensitive to rapamycin in vivo and the kinase readily phosphorylates 40S ribosomal subunit protein S6 in vitro. Also the growth factor treatment of Drosophila cells in culture induces S6 phosphorylation in an ordered fashion on five carboxylterminal residues [START_REF] Stewart | The Drosophila p70s6k homolog exhibits conserved regulatory elements and rapamycin sensitivity[END_REF][START_REF] Radimerski | Identification of insulin-induced sites of ribosomal protein S6 phosphorylation in Drosophila melanogaster[END_REF].

It has been known in enormous literature, the mammalian signaling pathways are largely conserved in Drosophila which offers its powerful genetic system to uncover novel components in signaling cascades. Therefore, to elucidate the cell growth functioning of dS6K, Montagne et al., did a pioneer work and identified a P-element insertion in the 5" non-coding region of the dS6K gene. They described that this mutation led to the strongly reduced female fertility and a developmental delay of about 3-days.

Furthermore, only 25% of the expected number of homozygous flies emerged as adults.

As the P-element insertion did not completely abolish dS6K transcription, null alleles were generated by imprecise excision of the transposable element. The homozygotes with a dS6K null mutation (dS6K l/l ) displayed a strong developmental delay of 5-days and were reduced in overall body size (Fig. 10). Montagne et al. noticed that the reduced size-phenotype also comprised of female sterility, inability to fly and reduced adult lifespan. Most importantly, they suggested that the flies lacking dS6K were reduced in overall body size due to smaller but not less cells. Moreover, the cell cycle phasing (G1/G2 distribution) in flies lacking dS6K was normal. However, the cells appeared to proliferate at a reduced rate and divided at a smaller size. Furthermore, analysis of the developing wing disc revealed that the effect on cell size was displayed throughout development [START_REF] Montagne | Drosophila S6 kinase: a regulator of cell size[END_REF].

As described above elsewhere, S6K is implicated in the regulation of translational components that make up the protein synthetic apparatus. The dS6K mutants were speculated to mimic ribosomal protein mutants, termed Minutes, a name derived from the strong reduction in bristle size [START_REF] Lambertsson | The minute genes in Drosophila and their molecular functions[END_REF]. Minutes are classified as recessive lethal mutations in ribosomal protein genes, which lead to dominant phenotypes in heterozygotes. The resulting phenotype includes a delay in development as well as short and slender bristles despite of normal overall body size. As bristle formation requires strong protein synthesis, bristle size can be viewed as a marker for translational capacity of the cell that generates it. Interestingly, the dS6K mutant phenotype was quite distinct from that of Minutes. Both of the mutations displayed a delay in development and comprised of reduced ribosome content. However, the dS6K mutants did not exhibit a strong reduction in bristle size [START_REF] Montagne | Drosophila S6 kinase: a regulator of cell size[END_REF]. The phenotypical differences compared to Minutes were rather inconsistent with dS6K solely regulating ribosome production. This is to mention that the absence of dS6K, possibly due to lack of S6 phosphorylation affects both the ribosome biogenesis and the individual phase of translation. Given the relatively strong phenotype of dS6K null flies, it was surprising that targeted overexpression of dS6K in various tissues using the Gal4-UAS system did not induce any obvious phenotype except in the dorsal wing compartment when transgene expression was driven with apterous-Gal4 (ap-Gal4; the apterous promoter is restricted to the dorsal compartment of the wing). The ap-Gal4

directed UAS-dS6K expression forces the wing to bend downwards as the dorsal and ventral epithelial sheaths form the adult wing, suggesting that the expression of an extra copy of the dS6K gene in the dorsal compartment of the wing caused only those cells to grow larger and not the cells in the ventral compartment. Although the resulted phenotype due to dS6K overexpression was very impressive; however, it should be pointed-out that very subtle changes in cell growth could account for the bending down of the wing. In fact, less than 1% increase in the size of the dorsal surface versus the ventral surface would be sufficient to induce such phenotype. Furthermore, Wu et al.

described the role of hunger-driven behaviours which are actually regulated by neural ribosomal dS6K [START_REF] Wu | Regulation of hunger driven behaviors by neural ribosomal S6 kinase in Drosophila[END_REF]. Also, Vargas et al. defined the role of dS6K in maintaining the dietary switch followed by mating and then increased preference for nutrients such as yeast; a major protein source. They suggested that TOR signaling plays an important role in maintaining a nutrient balance in Drosophila [START_REF] Vargas | A role of S6 kinase an Serotonin i postmating dietary switch and balance of nutrients in D. Melanogaster[END_REF].

To summarize, in fact; the TOR signaling pathway regulates a balance of nutrients [START_REF] Vargas | A role of S6 kinase an Serotonin i postmating dietary switch and balance of nutrients in D. Melanogaster[END_REF]. But, more specifically to the viewpoint of dS6K and growth; the most important and pioneer work has been demonstrated by Montagne et al., which shows that the dS6K regulates cell size, growth, and proliferation in a cell autonomous manner without impinging on cell number [START_REF] Montagne | Drosophila S6 kinase: a regulator of cell size[END_REF].

-Archipelago: A key regulator of protein degradation

The regulation of tissue growth requires a precise control of gene expression by which the developmental program generates an organism of a characteristic size and form [START_REF] Hariharan | Regulation of imaginal disc growth by tumorsuppressor genes in Drosophila[END_REF]. A significant feature of all living organisms is their utilization of proteins to construct molecular machineries that undertake the complex network of cellular activities. The posttranscriptional control of protein abundance has recently emerged as an important aspect of developmental biology [START_REF] Ho | The utility F-box for protein destruction[END_REF].

Perturbations of the mechanisms that regulate normal growth are observed in disease states caused by an aberrant growth such as cancer. By characterizing these abnormal features that can alter the normal regulation of tissue growth, the signaling pathways could be reconstructed that function together to control the growth process. These pathways form the crucial link between the patterning mechanisms which specify the overall form of the organism and the cellular pathways that regulate growth [START_REF] Hariharan | Regulation of imaginal disc growth by tumorsuppressor genes in Drosophila[END_REF]. In Drosophila, mutations that alter tissue growth have provided a valuable entry points into the study of growth regulatory pathways. The tissue overgrowth almost always results from a perturbation of specific pathways that function to restrict growth and maintain normal organ size. Therefore, studies of mutations in genes that can be termed growth-suppressors or tumors-suppressors have advanced our understanding of the genetic regulation of tissue growth in Drosophila and mammals [START_REF] Vidal | Drosophila models for cancer research[END_REF][START_REF] Hariharan | Yeast, flies, worms, and fish in the study of human disease[END_REF].

The cellular activities in eukaryotes are organized in a coordinated fashion through complex interrelating networks maintained largely by generation and destruction of proteins. An important index for accomplishing this cellular balance is the dynamic protein levels [START_REF] Ho | The utility F-box for protein destruction[END_REF][START_REF] Tetzlaff | Defective cardiovascular development and elevated cyclin E and Notch proteins in mice lacking the Fbw7 F-box protein[END_REF]. By regulated processes like synthesis and degradation, protein turnover are under delicate temporal and spatial controls. In particular, the regulation of protein degradation is of great importance and although it has been studied less than protein synthesis. One major route of protein destruction is coordinated by a set of conserved molecules, the F-box proteins, which recruit specific substrates and regulate their abundance in the context of SCF-E3 ligases (see below).

-The F-box hypothesis

During 1990s, a region of homology was initially observed in proteins containing βtransducin repeats like Cdc4, Met30, and β-TrCP [START_REF] Kumar | The sulfur controller-2 negative regulatory gene of Neurospora crassa encodes a protein with beta-transducin repeats[END_REF]. This homologous sequence was not characterized until the discovery of a novel gene, Skp1p [START_REF] Bai | SKP1 connects cell cycle regulators to the ubiquitin proteolysis machinery through a novel motif, the F-box[END_REF]. Moreover, two-hybrid assay conducted for Cyclin F-binding proteins identified the human counterpart Skp1 [START_REF] Durfee | The retinoblastoma protein associates with the protein phosphatase type 1 catalytic subunit[END_REF]. Skp1 genes are crucial for cell cycle regulation and influential in determining the stability of certain proteins.

Alignments of Skp1p interacting protein sequences by Elledge and colleagues revealed a 40-amino acid sequence required for binding to Skp1p. It was not until then that a name, F-box was coined for this motif, and subsequently onward the F-box hypothesis arose.

The term F-box was named after Cyclin F, the first defined F-box protein (FBP) [START_REF] Bai | SKP1 connects cell cycle regulators to the ubiquitin proteolysis machinery through a novel motif, the F-box[END_REF]. Soon after, proteins carrying the F-box motif were found to be evolutionarily conserved in various species. To date, numbers of identified FBPs range from about 11 in Saccharomyces cerevisiae, 33 in Drosophila melanogaster (Table 1) and approximately 600 in Arabidopsis thaliana. F-box proteins are evolutionally conserved among eukaryotes like C. elegans and D. melanogaster, H. sapiens, S. cerevisiae [START_REF] Ho | F-box proteins: the key to protein degradation[END_REF][START_REF] Ho | The utility F-box for protein destruction[END_REF].

The importance of F-box proteins are being dictated in several ways. F-box proteins (FBPs) are responsible for the substrate specificity of SCF complexes (Skp1, Cul1, and FBP) (Fig. 11) that serve as the variable adaptors to recruit specific protein substrates to SCF complexes [START_REF] Skowyra | F-box proteins are receptors that recruit phosphorylated substrates to the SCF ubiquitin-ligase complex[END_REF]. FBPs proteins connect the ubiquitination machinery and diverse cellular processes by exerting control over the stability of substrate proteins. FBPs also function in many different biochemical contexts and are involved in DNA replication, transcription, cell differentiation and cell death [START_REF] Ho | F-box proteins: the key to protein degradation[END_REF][START_REF] Kanemori | Regulation of the cell cycle and checkpoint by SCF(beta-TrCP)][END_REF].

-Phosphorylation is required for FBP target recognition

FBPs in SCF complexes are known to provide specificity when deciding which substrate is going to be degraded. Mostly, the substrate phosphorylation is a common prerequisite for FBP target recognition. It is critical that one or more residues of the substrate are phosphorylated prior to the FBP-substrate interaction. For example, the two serine residues in the motif need to be phosphorylated sequentially before binding to β-TrCP [START_REF] Spencer | Signal-induced ubiquitination of IkappaBalpha by the F-box protein Slimb/beta-TrCP[END_REF]. Another well-known mammalian FBP, Fbw7/hCdc4, recognizes LLTPPQSG sequence deduced from Cyclin E [START_REF] Ho | The utility F-box for protein destruction[END_REF][START_REF] Orlicky | Structural basis for phosphodependent substrate selection and orientation by the SCFCdc4 ubiquitin ligase[END_REF][START_REF] Moberg | Archipelago regulates Cyclin E levels in Drosophila and is mutated in human cancer cell lines[END_REF]. Within the Cyclin E, Thr-380 is the crucial phospho-residue with an immediately adjacent proline residue. Some Fbw7 substrates also carry a phosphorylated serine at the +4 position after the threonine. Many substrates include more than one serine/threonine (S/T) residues, as in the case of Sic1p which is the substrate of the yeast Cdc4p. Therefore, the sequential phosphorylation events are important for substrate binding and hence their degradation [START_REF] Ho | The utility F-box for protein destruction[END_REF].

-Ubiquitination and proteasomal degradation

The proteins targeted for degradation by the proteasomal pathway are first ubiquitinated.

i.e., a small molecule ubiquitin (Ub) is covalently attached to the protein [START_REF] Pickart | Back to the future with ubiquitin[END_REF].

Ubiquitination plays a key regulatory role in the Ub-proteasomal degradation pathway which involves three major enzymes: E1 activating enzyme, E2 conjugating enzyme, and E3 ubiquitin ligases. E1 and E2 enzymes are responsible for activating and conjugating the Ub moieties, respectively, whereas E3 ligases are important for transferring Ub moieties from the E2 enzyme onto the recognized substrates destined for destruction by the 26S proteasome [START_REF] Pickart | Mechanisms underlying ubiquitination[END_REF][START_REF] Cardozo | The SCF ubiquitin ligase: insights into a molecular machine[END_REF]. Two types of E3 ligases, HECT-domain and RING-domain type (Really Interesting New Gene) are known which utilize different mechanisms to transfer the Ub adducts to substrates [START_REF] Pickart | Mechanisms underlying ubiquitination[END_REF][START_REF] Petroski | In vitro reconstitution of SCF substrate ubiquitination with purified proteins[END_REF]. The RING-domain E3s transfer Ub directly onto substrates from the E2 enzymes; whereas, HECT-domain E3s act slightly different, i.e. the Ub is transferred first to the HECT E3s, and then targeted to the substrates (Fig. 11).

The best characterized RING ligases, the SCF (Skp-Cullin-F-box) complex, is composed of four major components: Skp1, Cul1/Cdc53, Roc1/Rbx1/Hrt1, and an F-box protein (Fig. 11) [START_REF] Ho | F-box proteins: the key to protein degradation[END_REF][START_REF] Cardozo | The SCF ubiquitin ligase: insights into a molecular machine[END_REF]. Within the SCF complex, Cul1 is the scaffold protein that interacts with Roc1 at its carboxyl-terminus to recruit

Ub-conjugated E2. The amino-terminus of Cul1 interacts with Skp1 which binds to the F-box motif of an F-box protein (Fig. 11). The carboxyl-terminal part of the FBPs usually consists of substrate-binding domains of various types, such as Trp-Asp (WD) repeats and leucine-rich repeats (LRR), both of which have been demonstrated to bind to already phosphorylated substrates. Depending on the motifs at the carboxyl-terminus, FBPs are categorized into three major families (Table 1): the FBW family contains F-box proteins with WD repeats whereas those from the FBL family contain LRRs. The third family, FBX, consists of F-box proteins with other protein-protein interaction domains at the carboxyl-terminus such as carbohydrate-interacting (CASH), zinc-finger, and proline-rich domains [START_REF] Cardozo | The SCF ubiquitin ligase: insights into a molecular machine[END_REF]. (drawn from [START_REF] Ou | Control of protein degradation by E3 ubiquitin ligases in Drosophila eye development[END_REF][START_REF] Dubois-Dalcq | The neurobiology of Xlinked adrenoleukodystrophy, a demyelinating peroxisomal disorder[END_REF][START_REF] Ho | F-box proteins: the key to protein degradation[END_REF].

1.4.4-Molecular and biological function of Archipelago (ago)

Archipelago (ago) has been localized to position 64B on the left arm of chromosome 3 through meiotic and deletion mapping. All of the three ago alleles (ago 1 , ago 3 , ago 4 ) have mutations in an open reading frame designated CG15010 which has been demonstrated through sequencing of the transcriptional units. ago encodes a 1,326amino-acid protein that contains an F-box and seven WD repeats in its carboxy-terminal portion. As already described, F-boxes and WD motifs are found in those proteins which function as the substrate-recognition component of SCF-type ubiquitin ligase complexes.

The Ago protein in its C-terminal portion is most similar to that encoded by an aminoterminally truncated human expressed sequence tag (EST) previously designated FLJ11071, which has been further renamed as human Ago. This protein is also referred to as hCdc4. Furthermore, an homolog gene in Caenorhabditis elegans also exists which is named as sel-10 [START_REF] Hubbard | sel-10, a negative regulator of lin-12 activity in Caenorhabditis elegans, encodes a member of the CDC4 family of proteins[END_REF]. Two of the mutations (ago 4 : Ala 1,117 to Val, ago 3 : Gly 1,131 to Glu) are missense mutations that map to the fourth WD repeat. Each alters a residue that is conserved among the three proteins. The third mutation (ago 1 : Gln 1,195 to stop) results in premature termination of translation early in the sixth WD repeat (Fig. 12) [START_REF] Moberg | Archipelago regulates Cyclin E levels in Drosophila and is mutated in human cancer cell lines[END_REF]. Below here, some of the biological functions of ago are briefly discussed.

-Archipelago in cell cycle and genomic instability

Central to the cell developmental mechanism is the process of the cell cycle, a basic cyclic progression formed by repetitive events including the S phase for the replication of DNA and the M phase for initiation of genome segregation. Cells grow and divide through checkpoints at each stage of the cell cycle while correct timing is under the control of cyclin proteins and the serine/threonine protein kinases known as cyclindependent kinases (CDKs) [START_REF] Ekholm | Regulation of G(1) cyclin-dependent kinases in the mammalian cell cycle[END_REF]. The endocycle is a developmentally specialized cell cycle that lacks M phase and consists of only S and G phases. Endoreplicating cells acquire high ploidies by multiple rounds of replication without cell divisions. Following the cell proliferative phase of Drosophila embryogenesis, many tissues initiate endocycles that lack all visible aspects of mitosis.

These tissues include the gut, epidermis, fat body, malpighian tubules, trachea and salivary glands, which continue to endocycle during larval development long after they are fully differentiated. Some adult tissues, including ovarian follicle and nurse cells, and sensory neurons in the wing, also employ endocycles [START_REF] Asano | Endoreplication: the advantage to initiating DNA replication without the ORC?[END_REF]. One central regulator of all Drosophila endocycles is the S phase kinase, Cyclin E/Cdk2. The cyclin E gene is transcribed prior to the onset of endocycle S and is required for these cycles [START_REF] Knoblich | Cyclin E controls S phase progression and its down-regulation during Drosophila embryogenesis is required for the arrest of cell proliferation[END_REF]. Szuplewski et al presented a novel cell cycle regulator, called minus, which influences Cyclin E turnover in Drosophila and proposed that Minus contributes to cell cycle regulation in part by selectively controlling turnover of Cyclin E.

Minus protein has also been known to interact physically with Cyclin E and the SCF Archipelago/Fbw7/Cdc4 ubiquitin ligase complex [START_REF] Szuplewski | Drosophila Minus is required for cell proliferation and influences Cyclin E turnover[END_REF]. It has been described that Cyclin E is regulated by Ago (see below) [START_REF] Moberg | Archipelago regulates Cyclin E levels in Drosophila and is mutated in human cancer cell lines[END_REF] and hence, perturbation in Ago causes a continuous overexpression of Cyclin E which inhibits endocycle progression in Drosophila and eventually causes a genomic instability [START_REF] Follette | Fluctuations in cyclin E levels are required for multiple rounds of endocycle S phase in Drosophila[END_REF][START_REF] Weiss | Continuous Cyclin E expression inhibits progression through endoreduplication cycles in Drosophila[END_REF][START_REF] Edgar | Endoreplication cell cycles: more for less[END_REF]. Ago [START_REF] Moberg | Archipelago regulates Cyclin E levels in Drosophila and is mutated in human cancer cell lines[END_REF].

-Archipelago as a regulator of Cyclin E and dMyc

Myc genes encode basic-helix-loop-helix-zipper (bHLHZ) domain transcription factors and dimerize with Max family proteins through the bHLHZ domain to promote cell growth and proliferation [START_REF] Grandori | The Myc/Max/Mad network and the transcriptional control of cell behavior[END_REF][START_REF] Donaldson | Cancer cell biology: Myc wins the competition[END_REF]. In Drosophila, overexpression of dMyc causes cell over growth as evidenced through an increased size of developing tissues [START_REF] Neufeld | Connections between growth and the cell cycle[END_REF]Johnston and Gallan, 2002). Moberg et al. performed interaction screens using the Ago F box/WD domain as a bait to search for additional substrates of Ago and recovered dMyc [START_REF] Moberg | The Drosophila F box protein archipelago regulates dMyc protein levels in vivo[END_REF].

While both dMyc and Cyclin E promote S phase entry, only dMyc was found to have effect on growth [START_REF] Neufeld | Connections between growth and the cell cycle[END_REF]. As overexpression of dMyc causes cell over growth, the phenotype was recapitulated and found to be consistent with ago mutant tissue phenotypes. The dMyc mutant flies were smaller than the wild-type flies in body size, and losing a copy of wild-type ago gene under the dMyc mutant background restores the body size defect to the extent of 12 to 15%. Similar to Cyclin E, protein levels of dMyc were also elevated in ago mutant clones and its mRNA level remains the same. Direct interaction between dMyc and Ago were also observed by cell transfection and co-immunoprecipitation analyses [START_REF] Moberg | The Drosophila F box protein archipelago regulates dMyc protein levels in vivo[END_REF]. To summarize in brief, Ago regulates the degradation of dMyc and the increased cell growth observed in tissues which ectopically expressed dMyc mainly accounts for the ago mutant phenotype.

-Archipelago and tracheogenesis

In Drosophila melanogaster, tubular morphogenesis underlies formation of the tracheal system, a network of interconnected tubes that duct air throughout the developing organism. The central components of the Drosophila fibroblast growth factor (FGF)

pathway associated with tracheogenesis are encoded by the breathless (btl) and branchless (bnl) genes, which encode an FGF-like receptor and an FGF-like secreted ligand respectively [START_REF] Klambt | breathless, a Drosophila FGF receptor homolog, is essential for migration of tracheal and specific midline glial cells[END_REF][START_REF] Sutherland | branchless encodes a Drosophila FGF homolog that controls tracheal cell migration and the pattern of branching[END_REF]. The role of this receptor/ligand pair in controlling tracheal outgrowth is based upon a simple model in which the restricted expression of bnl in cells outside the tracheal placode represents a directional cue for the migration of btl-expressing cells within the tracheal placode, which is dependent upon the trachealess (trh) gene [START_REF] Isaac | Tubulogenesis in Drosophila: a requirement for the trachealess gene product[END_REF]. Mutation of any one of these core components; btl, bnl, or trh produces a failure of tracheal branching. Mortimer and Moberg showed that Ago acts upstream of trachealess (Trh) and breathless (btl) and is required for controlling the levels of trachealess (Trh) transcription factor. Ago binds directly to Trh and stimulates its proteasomal degradation [START_REF] Mortimer | The Drosophila F-box protein Archipelago controls levels of the Trachealess transcription factor in the embryonic tracheal system[END_REF]. This novel role for Ago in tracheal development is supported by an independent finding which demonstrated that the homozygosity for a genomic deletion containing ago locus is associated with cell migration defects in embryonic tracheal development [START_REF] Myat | Making tubes in the Drosophila embryo[END_REF]. dVHL (Von Hippel-Lindau) protein is a central player in the hypoxic response pathway in embryonic and larval tracheal cells and has been revealed to strongly interact with Ago. dVHL is a substrate adaptor component of an ubiquitin ligase that subsequently polyubiquitinates HIF-1α (Hypoxia induced factor) and targets it for degradation by the proteasome. Thus, both of these two proteins, dVHL and Ago synergize with each other to regulate the process of tracheogenesis in Drosophila [START_REF] Mortimer | Regulation of Drosophila embryonic tracheogenesis by dVHL and hypoxia[END_REF].

-Archipelago controls Rb/E2F regulated apoptosis

Recently, Nicholson et al. found that due to unrecognized mechanism the loss of ago has different effects on the size of different tissues in the adult head. There is no solid reason to elaborate that ago loss induces hyperplasia of some of organs but paradoxically reduces the size of the adult eye. This reflects a requirement for ago to restrict apoptotic activity of the rb1/de2f1 pathway. Adjacent to the eye-specific morphogenetic furrow (MF), ago mutant cells displayed an elevated level of dE2f1 activity and expressed the prodeath dE2f1 targets hid and rpr. Furthermore, the ago mutant cells also showed high rates of apoptosis [START_REF] Nicholson | The archipelago tumor suppressor gene limits rb/e2f-regulated apoptosis in developing Drosophila tissues[END_REF]. Eye overgrowth resulting from loss of ago was retarded by apoptosis that could occur as ago mutant cells encounter the MF.

Blocking this death led to eye hypertrophy, demonstrating that ago mutations could synergize with anti-apoptotic mutations to drive organ overgrowth. The pattern of apoptosis in ago mutant eye discs resembled that caused by loss of the retinoblastoma homolog rbf1 [START_REF] Moon | A gradient of epidermal growth factor receptor signaling determines the sensitivity of rbf1 mutant cells to E2Fdependent apoptosis[END_REF]. In summary, these data linked two tumor suppressor genes ago and rbf1 in a common developmental pathway and also displayed a role of ago as a required regulator of the rbf1/de2f1 pathway.

-Archipelago as a tumour suppressor

The regulation of tissue growth is, perhaps, the most important means by which the developmental program generates an organism of a characteristic size and form. The perturbations in the mechanisms that regulate normal growth lead to an aberrant growth such as cancer [START_REF] Hariharan | Regulation of imaginal disc growth by tumorsuppressor genes in Drosophila[END_REF]. In Drosophila, analysis of the growth restrictive role of archipelago in proliferating larval imaginal disc cells has provided an excellent model to understand the tumor suppressive properties of ago mammalian orthologs [START_REF] Moberg | The Drosophila F box protein archipelago regulates dMyc protein levels in vivo[END_REF]. Elevated levels of Cyclin E have been reported in a subset of human tumors, notably of the breast and ovary [START_REF] Keyomarsi | The role of cyclin E in cell proliferation, development and cancer[END_REF]. Moberg et al. examined human ovarian cancer cell lines and demonstrated that, 3 out of 10 had mutations in the human Fbw7 gene, the homolog of ago. The Cyclin E was upregulated in these ovarian cancer cell lines as well, implicating a role of ago in human cancer pathogenesis [START_REF] Moberg | Archipelago regulates Cyclin E levels in Drosophila and is mutated in human cancer cell lines[END_REF]. Spruck et al. observed that the F-box protein hCdc4 was mutated in at least 16% of human endometrial tumors. Furthermore, mutations were found either in the substrate-binding domain of the protein or at the amino terminus, suggesting a critical role for the region of hCdc4 upstream of the F-box [START_REF] Spruck | hCDC4 gene mutations in endometrial cancer[END_REF]. In addition, there are many reports which show that this highly conserved mammalian ago ortholog (variously termed Fbw7, Fbxw7, hAgo, hCDC4, or hSel-10) is a mutational target in a rapidly expanding array of human cancers [START_REF] Balakrishnan | Novel somatic and germline mutations in cancer candidate genes in glioblastoma, melanoma, and pancreatic carcinoma[END_REF][START_REF] Calhoun | BRAF and FBXW7 (CDC4, FBW7, AGO, SEL10) mutations in distinct subsets of pancreatic cancer: potential therapeutic targets[END_REF][START_REF] Malyukova | The tumor suppressor gene hCDC4 is frequently mutated in human T-cell acute lymphoblastic leukemia with functional consequences for Notch signaling[END_REF][START_REF] Minella | p53 and SCFFbw7 cooperatively restrain cyclin E-associated genome instability[END_REF].

-RNAi Screening for regulators of growth and metabolism

The fruit fly, Drosophila melanogaster has been a valuable model organism in which genetic screens have been used to study a variety of cellular and developmental processes for close to a century [START_REF] Nagy | Tailoring the genome: the power of genetic approaches[END_REF][START_REF] St Johnston | The art and design of genetic screens: Drosophila melanogaster[END_REF]. Traditionally, the power of Drosophila genetics relied on forward genetic screens to identify genes which affect a given biological process. Forward genetic screens uncover many genes required for specific processes, but other genes can be missed due to limitations of the screening process. For example, embryonic screens may not uncover some mutations because the genes are expressed during oogenesis contributed by the mother and mask a mutation in the zygotic gene [START_REF] St Johnston | The art and design of genetic screens: Drosophila melanogaster[END_REF]. Patterning of the early embryo body axes is directed by mRNAs that are deposited in the oocyte by the mother and localized at the specific positions within the embryo. The genes providing the mRNA are known as the maternal effect genes as they are expressed in the mother rather than the embryo.

Moreover, of the 14,600 predicted open reading frames in Drosophila genome, only approximately 3600 have been associated with mutant phenotypes. It is not entirely clear why so many genes have been missed in forward genetic screens [START_REF] Adams | The genome sequence of Drosophila melanogaster[END_REF][START_REF] Kuttenkeuler | Genome-wide RNAi as a route to gene function in Drosophila[END_REF]. Recently, the availability of Drosophila genome sequences enables a genetic approach to be utilized to determine the function of predicted classes of gene products [START_REF] Mohr | Genomic screening with RNAi: results and challenges[END_REF]. Since many developmental processes as well as known human disease genes are conserved between flies and vertebrates [START_REF] St Johnston | The art and design of genetic screens: Drosophila melanogaster[END_REF], studies in the fly may provide useful insights into understanding the signaling pathways and also the causes of diseases in humans [START_REF] Hietakangas | Regulation of tissue growth through nutrient sensing[END_REF]. Hence, taking advantage of the conserved genomic analogy between fly and humans and also the availability of RNAi library, a reverse genetic approach through an RNAi screen has been followed-out to look for regulators of growth, development and metabolism in Drsosophila melanogaster. A short note on RNAi and its utility in drosophila is briefly discussed below.

-RNA interference (RNAi) and its mechanism

RNA interference (RNAi) is a mechanism for directly knocking down expression of genes with known sequence and has proven to be a powerful reverse genetic approach. [START_REF] Fire | RNA-triggered gene silencing[END_REF][START_REF] Fire | Gene silencing by double-stranded RNA[END_REF]. RNAi was originally discovered in Caenorhabditis elegans when injection of double-stranded RNA (dsRNA) into the worm was shown to result in degradation of the corresponding mRNA and silencing of the gene product [START_REF] Fire | Potent and specific genetic interference by double-stranded RNA in Caenorhabditis elegans[END_REF]. Injection with dsRNA proved to be much more potent in knocking down mRNA levels than injection with either sense or anti-sense RNA alone. In addition to injection of dsRNA, RNAi has been demonstrated in C. elegans by feeding on E. coli engineered to express dsRNA of a nematode gene [START_REF] Timmons | Specific interference by ingested dsRNA[END_REF] and soaking the worms in a solution of dsRNA [START_REF] Tabara | RNAi in C. elegans: soaking in the genome sequence[END_REF].

RNA interference refers to the use of double-stranded RNA (dsRNA) to target mRNAs for degradation leading to silencing of gene expression. The dsRNA sequence is utilized by RNA machinery to produce a protein-RNA complex that degrades the corresponding mRNA, thereby producing an extremely specific means of silencing genes, as other nonspecific mRNAs are not affected [START_REF] Zamore | RNAi: doublestranded RNA directs the ATP-dependent cleavage of mRNA at 21 to 23 nucleotide intervals[END_REF]. It has been proposed that the biological roles of RNAi may be to maintain the integrity of the genome by suppressing mobilization of transposons or to prevent an accumulation of repetitive sequences in the germline [START_REF] Aravin | Double-stranded RNA-mediated silencing of genomic tandem repeats and transposable elements in the D. melanogaster germline[END_REF]. In addition, RNAi is speculated to be a defence against viral infections as well as a regulator of gene expression [START_REF] Zamore | RNA interference: listening to the sound of silence[END_REF].

RNA interference is a two-step process. The RNAi pathway is initiated through ATPdependent cleavage of the dsRNA into small interfering RNAs (siRNAs) 21-25 nt in length by Dicer, a Ribonuclease (RNaseIII), [START_REF] Bernstein | Role for a bidentate ribonuclease in the initiation step of RNA interference[END_REF][START_REF] Elbashir | RNA interference is mediated by 21-and 22-nucleotide RNAs[END_REF][START_REF] Zamore | RNAi: doublestranded RNA directs the ATP-dependent cleavage of mRNA at 21 to 23 nucleotide intervals[END_REF]. Dicer is a member of the RNaseIII family of dsRNA specific endonucleases [START_REF] Bernstein | Role for a bidentate ribonuclease in the initiation step of RNA interference[END_REF], and similar to other family members produces a small interfering RNAs (siRNA) product consisting of a 5′ phosphate and 3′ hydroxyl with two unpaired nucleotides at each end [START_REF] Elbashir | RNA interference is mediated by 21-and 22-nucleotide RNAs[END_REF]. The siRNAs then interacts with a multi-protein complex that is not able to mediate RNAi until the ATPdependent unwinding of the siRNA duplex is initiated [START_REF] Nykanen | ATP requirements and small interfering RNA structure in the RNA interference pathway[END_REF]. Upon duplex unwinding, the complex undergoes a conformational change producing an active RNA induced silencing complex (RISC) (Fig. 13). The siRNA antisense strand of the activated RISC complex can recognize and hybridize with corresponding target mRNA leading to cleavage of the mRNA by the RISC complex [START_REF] Hammond | An RNAdirected nuclease mediates post-transcriptional gene silencing in Drosophila cells[END_REF][START_REF] Nykanen | ATP requirements and small interfering RNA structure in the RNA interference pathway[END_REF] and subsequently, regulating the expression of a protein-coding gene [START_REF] Hannon | RNA interference by short hairpin RNAs expressed in vertebrate cells[END_REF].

-Utility of RNAi technique

The availability of genomic sequences from Caenorhabditis elegans, Drosophila melanogaster, Mus musculus and human have provided a source of target sequences against which RNAi can be utilized as a reverse genetic tool to begin understanding the function of many genes with previously unknown function. RNAi has been effectively used as a tool to perform multiple screens in C. elegans [START_REF] Gonczy | Functional genomic analysis of cell division in C. elegans using RNAi of genes on chromosome III[END_REF],

Drosophila embryos [START_REF] Ivanov | T-cadherin upregulation correlates with cell-cycle progression and promotes proliferation of vascular cells[END_REF][START_REF] Kim | A functional genomic screen for cardiogenic genes using RNA interference in developing Drosophila embryos[END_REF][START_REF] Kuttenkeuler | Genome-wide RNAi as a route to gene function in Drosophila[END_REF], and Drosophila cell culture to identify genes involved in numerous biological processes including cell cycle regulators, cell morphology, growth and viability and signaling pathways [START_REF] Bai | Culture of Drosophila primary cells dissociated from gastrula embryos and their use in RNAi screening[END_REF][START_REF] Doumanis | RNAi screening in Drosophila cells identifies new modifiers of mutant huntingtin aggregation[END_REF][START_REF] Bjorklund | Identification of pathways regulating cell size and cell-cycle progression by RNAi[END_REF][START_REF] Clemens | Use of double-stranded RNA interference in Drosophila cell lines to dissect signal transduction pathways[END_REF]. RNAi techniques both in vivo and in cultured cells have been increasingly used to explore functions of uncharacterized genes by loss-of-function analysis [START_REF] Kuttenkeuler | Genome-wide RNAi as a route to gene function in Drosophila[END_REF]). The first large-scale RNAi screens covering single chromosomes of C. elegans genome identified 90 percent of embryonic lethal alleles that were known from previous studies and provided evidence for the efficiency of RNAi approaches [START_REF] Fraser | Functional genomic analysis of C. elegans chromosome I by systematic RNA interference[END_REF][START_REF] Gonczy | Functional genomic analysis of cell division in C. elegans using RNAi of genes on chromosome III[END_REF]. Subsequent genome-wide screens for developmental defects identified phenotypes for more than 1,700 genes in the C. elegans genome [START_REF] Kamath | Systematic functional analysis of the Caenorhabditis elegans genome using RNAi[END_REF]. Therefore, genome-wide RNAi screens in C.

elegans impressively demonstrated the versatility of this approach and demonstrated how biologically significant insights can be gained using a variety of phenotype based assays [START_REF] Kuttenkeuler | Genome-wide RNAi as a route to gene function in Drosophila[END_REF].

-Drosophila: A model for genetic screens

Experimentally introduced dsRNA can trigger the RNAi effect in a wide range of experimental organisms, providing a powerful method for disrupting gene function [START_REF] Fire | Potent and specific genetic interference by double-stranded RNA in Caenorhabditis elegans[END_REF]. Drosophila is one of the major model organisms in which genetic screens have been used to study a variety of cellular and developmental processes [START_REF] St Johnston | The art and design of genetic screens: Drosophila melanogaster[END_REF][START_REF] Nagy | Tailoring the genome: the power of genetic approaches[END_REF]. In Drosophila, several methods have been used to deliver dsRNA. The simplest method works for cultured cells; dsRNA is added directly to the culture medium which silences gene function within 72 h. This can be a powerful method for working out signal transduction pathways, cell biological phenomena and the mechanism of RNAi itself [START_REF] Adams | Essential roles of Drosophila inner centromere protein (INCENP) and aurora B in histone H3 phosphorylation, metaphase chromosome alignment, kinetochore disjunction, and chromosome segregation[END_REF][START_REF] Giet | Drosophila aurora B kinase is required for histone H3 phosphorylation and condensin recruitment during chromosome condensation and to organize the central spindle during cytokinesis[END_REF][START_REF] Adams | From sequence to phenotype: reverse genetics in Drosophila melanogaster[END_REF][START_REF] Caplen | dsRNA-mediated gene silencing in cultured Drosophila cells: a tissue culture model for the analysis of RNA interference[END_REF][START_REF] Clemens | Use of double-stranded RNA interference in Drosophila cell lines to dissect signal transduction pathways[END_REF]. Two methods that use RNAi have been developed in Drosophila. The first relies on the delivery of dsRNA to embryos by microinjection. The main advantage of this method is that both maternally supplied mRNA and zygotically expressed mRNA are degraded. Also, the results are obtained within a few days of injection but the injected dsRNA might not produce a phenotype in every embryo, and the severity of the phenotype might vary between embryos [START_REF] Kennerdell | Heritable gene silencing in Drosophila using double-stranded RNA[END_REF][START_REF] Adams | From sequence to phenotype: reverse genetics in Drosophila melanogaster[END_REF]. Furthermore, the injection procedure itself can cause aberrant phenotypes or death in some embryos.

Unfortunately, microinjection of dsRNA into Drosophila embryos does not efficiently interfere with gene function later in development. An alternative that works at all stages and in all tissues is to generate the dsRNA in vivo [START_REF] Kennerdell | Heritable gene silencing in Drosophila using double-stranded RNA[END_REF]. This can be accomplished by expressing an RNA with a long inverted repeat that can fold back on itself to become double stranded. A principal advantage of this promising RNAi approach is the possibility of interfering with gene function in specific tissues or at specific times during development by using the Gal4-UAS system (Fig. 14) [START_REF] Brand | Targeted gene expression as a means of altering cell fates and generating dominant phenotypes[END_REF]. In order to assess phenotypes on a genome-wide scale, an RNAi library was constructed comprising 21,000 dsRNAs that covered all predicted open reading frames in the Drosophila genome to comprehensively characterize cell growth and survival phenotypes [START_REF] Hild | An integrated gene annotation and transcriptional profiling approach towards the full gene content of the Drosophila genome[END_REF]. Using approximately 4,400 dsRNAs derived from the Drosophila expressed sequence tag project, Lum et al. searched for Hedgehog pathway components using a luciferase reporter gene assay. They screened for both negative regulators of the pathway, which could activate the reporter gene in absence of a stimulus, and for positive regulators that are required to transmit the signal from the membrane to the nucleus. By these means, they identified several known and novel components, including casein kinase 1α, which has also been implicated in Wnt signaling [START_REF] Lum | Identification of Hedgehog pathway components by RNAi in Drosophila cultured cells[END_REF]. Wnt and Hedgehog pathways, which were first discovered as regulators of early embryonic development, but later have been hown to be indispensable for many other processes during development and disease in higher vertebrates [START_REF] Pospisilik | Drosophila genome-wide obesity screen reveals hedgehog as a determinant of brown versus white adipose cell fate[END_REF].

In genetic screens for early embryonic defects, independent alleles with similar phenotypes often uncovered multiple components in the same cellular pathway [START_REF] Kennerdell | Use of dsRNA-mediated genetic interference to demonstrate that frizzled and frizzled 2 act in the wingless pathway[END_REF]. Furthermore, many genes are used at multiple developmental stages enabling phenotypes of lethal mutations to be analyzed in embryos, but there are drawbacks to analyzing embryonic phenotypes [START_REF] St Johnston | The art and design of genetic screens: Drosophila melanogaster[END_REF]. Therefore, classical genetics may be limited by its inability to identify zygotic and maternal effects mutations simultaneously, and many mutations were not uncovered.

Nevertheless, RNAi has been shown to reduce levels of both maternal and zygotic mRNA enabling further analysis of these previously uncharacterized gene products [START_REF] Adams | From sequence to phenotype: reverse genetics in Drosophila melanogaster[END_REF].

A genetic screen for growth regulators in Drosophila revealed a new member of the TOR signaling pathway, melted (Teleman et al., 2005b). This gene encodes a protein with a pleckstrin homology (PH) domain that is essential for its function. Melted can bind TSC1 and recruit the TSC1/2 complex to cell membranes, suggesting it can act upstream of TOR. The melted mutants have reduced lipids but display no apparent defects in circulating sugar levels. Although the mechanisms remain unclear, Melted may help to facilitate the phosphorylation of dFOXO and TSC2 by Akt in response to insulin input. Consistent with the proposal that Melted limits the dFOXO response, the dFOXO target 4E-BP is highly upregulated in starved melted mutants, and reduced dFOXO levels can suppress the lipid metabolic defects of melted mutants (Teleman et al., 2005b). The identification of melted homologs in C. elegans, mice, and humans sets the stage for further studies to understand its role in TOR and FOXO signaling as well as lipid metabolism.

Recently, most of the RNAi screening is being carried-out in cultured cells. Both in vivo and in vitro approaches have different advantages and constraints. RNAi in cultured cells has the advantage of speed, reduced phenotype complexity and easily quantifiable phenotypes and also cell culture models can provide a convenient platform to study signal transduction pathways, they fail to recapitulate the complexity of signaling within intact organs and/or in the whole organism [START_REF] Mohr | Genomic screening with RNAi: results and challenges[END_REF][START_REF] Doumanis | RNAi screening in Drosophila cells identifies new modifiers of mutant huntingtin aggregation[END_REF][START_REF] Kuttenkeuler | Genome-wide RNAi as a route to gene function in Drosophila[END_REF]. In contrast, the main disadvantage of large-scale and genome-wide genetic screens is that they are costly, labor intensive, and time consuming [START_REF] Aerts | Integrating computational biology and forward genetics in Drosophila[END_REF]. However, RNAi experiments in vivo provide immediate insights into biological processes in a relevant setting and the results seem to be more reliable and applicable at an organism level [START_REF] Aerts | Integrating computational biology and forward genetics in Drosophila[END_REF]. Therefore, the physiological significance of results obtained in tissue culture should be corroborated in an animal model system. Such studies have been greatly facilitated by the development of certain tools to either disrupt or over-express nearly any gene of interest in a genetically suitable model organism such as Drosophila [START_REF] Kuhnlein | Drosophila as a lipotoxicity model organism--more than a promise?[END_REF][START_REF] Grewal | Insulin/TOR signaling in growth and homeostasis: a view from the fly world[END_REF].

-RNAi screen for regulators of Steroidogenesis at the ring gland

Growth and metabolism are coordinated according to genetic and environmental cues.

Furthermore, multicellular organisms have to orchestrate the maintenance and adjustment of the correct proportions of organs and tissues throughout life. The accomplishment of this commitment mainly remains perplexing. However, systemic growth factors such as hormones which transmit signals to target cells are known to play an important part in this regulation. Nutrient availability is important for the production and secretion of hormones [START_REF] Ikeya | Nutrientdependent expression of insulin-like peptides from neuroendocrine cells in the CNS contributes to growth regulation in Drosophila[END_REF] and hence thereby regulating the whole scenario of growth, development and the metabolism processes.

In insects, as in all animals, many aspects of development are under hormonal control.

The most important insect hormones are the ecdysteroid molting hormone (EC), which is secreted from the prothoracic glands, and the sesquiterpenoid juvenile hormone (JH), which is secreted from the corpus allatum (CA). In the larvae of Drosophila, ecdysteroids and juvenile hormones are produced by the ring gland, which is composed of the prothoracic gland, the corpus allatum, and the corpora cardiaca (Fig. 15). Ecdysteroids are actually synthesized from the dietary cholesterol which is converted through a multistep enzymatic pathway to Ecdysone (E). The peripheral hydroxylation of Ecdysone leads to the biologically active compound 20-hydroxyecdysone (20-HE). The ecdysteroids are required for morphogenetic events and for cuticle deposition in embryos. During larval development, ecdysteroid levels rise briefly controlling the transition from one stage to the next. At the end of the third instar, a large pulse of molting hormone, the comittement peak apparently causes the epidermis to become committed to trigger the onset of metamorphosis. This process ends with another pulse of molting hormone; the prepupal or molting peak is responsible for adult head eversion and the differentiation of adult structures (Fig. 16). The precise timing of these events suggests that steroid hormone synthesis and/or degradation are tightly regulated. Any perturbation in the titer of ecdysteroids could lead to the changes in the phenotype. For instance, an RNAi to DHR3 directed in the prothoracic gland causes giant L3 larvae, a phenotype that could be rescued with ecdysone feeding (Parvy et al., unpublished results). DHR3 is a nuclear receptor and has been known for its positive regulation of growth [START_REF] Montagne | The nuclear receptor DHR3 modulates dS6 kinase-dependent growth in Drosophila[END_REF]. Furthermore, it has been shown that β-Ftz-F1 another nuclear receptor is necessary for synthesis of the steroidogenic enzymes [START_REF] Parvy | A role for hFTZ-F1 in regulating ecdysteroid titers during post-embryonic development in Drosophila melanogaster[END_REF]. More recently, Parvy et al (unpublished results) have shown that RNAi to other nuclear receptors also interfere with the progression of larval developmental steps, which opened new doors of the regulation of the ecdysteroid biogenesis and, hence the control of development in drosophila. As DHR3 regulates dS6K-dependent growth and also the dS6K has been shown to play a role in metabolism, it is conceivable that all these processes; growth, development and metabolism are connected with one another through different effectors. Consistently, it is known that nutrition modifies the timing of development by acting on the production of the ecdysone at the prothoracic gland (PG)

and that the TOR signaling plays a critical role in controlling this process. The levels of ecdysone are regulated by a neurohormone PTTH (prothoracicotropic hormone) [START_REF] Gilbert | Control and biochemical nature of the ecdysteroidogenic pathway[END_REF], which may regulate TOR signaling [START_REF] Layalle | The TOR pathway couples nutrition and developmental timing in Drosophila[END_REF].

Keeping in view this prospect, a genome wide RNAi screening project was designed for the regulators of growth, development and metabolism in drosophila. Meanwhile, National Institute of Genetics, Japan (NIG) started a mega project to launch an RNAi library containing inducible UAS-RNAi lines directed against every drosophila gene.

Thanks to a collaborative project between NIG and the platform of Centre de Génétique Moléculaire (CGM), we were able to utilise these UAS-RNAi lines to screen for regulators of the steroidogenesis. As the UAS-RNAi collection spans the whole genome of Drosophila, thereby, this RNAi library provided us a convenient mean for saturating genetic screen. We expressed these inducible RNAi lines in the ring gland by using phm-Gal4 driver. As already described, that an RNAi to DHR3 when expressed in the ring gland caused an increase the larval size. This way, the larval phenotype served as a tool to screen for novel regulators of development which potentially regulate the process of steroidogenesis. The novel candidates which regulate the process of development at different stages through controlling the ecdysteroid biogenesis could be further selected for future studies.

Figure 15 -The structure and location of the ring gland in drosophila larva

The ring gland is present only in the larval stage and degenerated during metamorphosis.

It is composed of the prothoracic gland, the corpus allatum, and the corpora cardiaca.

The prothoracic gland comprised of steroidogenic cells (red); the corpus allatum (blue) which produces the JH and the corpora cardiacum (green) which regulates certain neuropeptides and metabolic hormones are shown.

-RNAi screen for regulators of lipid metabolism at the fat body

The ability to manipulate the fly genome in virtually any way desired, combined with a range of newly available genomic resources, allow the researcher to define the molecular mechanisms of gene function at a level of resolution not possible in more complex organisms. The similar proteomic composition of lipid droplets and the regulation of many of the genes under starvation establish parallels between fly and mammalian system [START_REF] Gronke | Brummer lipase is an evolutionary conserved fat storage regulator in Drosophila[END_REF][START_REF] Zinke | Nutrient control of gene expression in Drosophila: microarray analysis of starvation and sugar-dependent response[END_REF]. This analogy of lipid physiology indicates that the studies in Drosophila could provide new insights into how lipid homeostasis is maintained [START_REF] Cermelli | The lipid-droplet proteome reveals that droplets are a protein-storage depot[END_REF]. The Drosophila larval fat body combines the hepatic and adipose functioning and hence, serves as a most sophisticated and convenient tissue for the dissection of metabolic function [START_REF] Colombani | A nutrient sensor mechanism controls Drosophila growth[END_REF].

Fat body cells accumulate large stores of carbohydrates, lipids and proteins that are normally used during metamorphosis. During the third instar of larvae, this accumulation of nutrients causes opacity of fat body cells. If the larva is starved, remobilization of these stored metabolites causes shrinkage of fat body cells which become clear. Close investigation of fat body cells reveals that starvation provokes large increase of cytoplasmic vesicles while after storage and; otherwise during normal feeding conditions many more vesicles are visible but their size is significantly reduced [START_REF] Hietakangas | Regulation of tissue growth through nutrient sensing[END_REF]. Moreover, it has been also reported that under starvation, in late larval stages and during metamorphosis, cytoplasmic components within the fat body are degraded by autophagy to provide nutrients to developing imaginal tissues [START_REF] Juhasz | The Drosophila homolog of Aut1 is essential for autophagy and development[END_REF][START_REF] Rusten | Programmed autophagy in the Drosophila fat body is induced by ecdysone through regulation of the PI3K pathway[END_REF]. Starvation has also been shown to induce alterations in the morphology of fat body cells, including aggregation of lipid droplets and mobilization of nutrient stores [START_REF] Britton | Drosophila's insulin/PI3-kinase pathway coordinates cellular metabolism with nutritional conditions[END_REF][START_REF] Colombani | A nutrient sensor mechanism controls Drosophila growth[END_REF][START_REF] Zhang | Regulation of cellular growth by the Drosophila target of rapamycin dTOR[END_REF], and to cause a marked reduction in fat body cell size [START_REF] Scott | Role and regulation of starvation-induced autophagy in the Drosophila fat body[END_REF]. Interestingly modulation of a number of growth or metabolic regulators [START_REF] Hietakangas | Regulation of tissue growth through nutrient sensing[END_REF][START_REF] Britton | Drosophila's insulin/PI3-kinase pathway coordinates cellular metabolism with nutritional conditions[END_REF][START_REF] Beller | COPI complex is a regulator of lipid homeostasis[END_REF] phenocopies the starvation induced phenotype in the fat body [START_REF] Diangelo | Regulation of fat cell mass by insulin in Drosophila melanogaster[END_REF]. These regulators include intermediates in the insulin and nutrient network, as well as amino acid transporters, lipase and microRNA [START_REF] Colombani | A nutrient sensor mechanism controls Drosophila growth[END_REF][START_REF] Britton | Drosophila's insulin/PI3-kinase pathway coordinates cellular metabolism with nutritional conditions[END_REF][START_REF] Teleman | Drosophila lacking microRNA miR-278 are defective in energy homeostasis[END_REF].

The modulating lipid droplet phenotype can serve as a phenotypic tool and a good readout to find new components of a signaling cascade and to further explore the metabolic functioning of genes [START_REF] Grewal | Insulin/TOR signaling in growth and homeostasis: a view from the fly world[END_REF]. Since, no studies to date have exploited one of the main strengths of Drosophila; the ability to conduct large-scale genome-wide genetic screen in larvae for regulators of lipid metabolism. We benefited the RNAi library of the NIG fly stock to screen for new regulators of metabolism. For the screening of novel regulators which are potentially implicated in the lipid metabolism, the larval fat body was used as a tool. Moreover; the easiness of dissection, the tools available for fat body screening and the easy detection of lipid droplets, we have thus decided to use the lipid droplet phenotype as a convenient tool to screen for specific regulators.

This screening approach holds the promising commitment of extending our understanding of the regulation of lipid metabolism in a new and unexpected direction. It is likely that we have much to look forward to in the next few years, as further studies exploit the humble fruit fly to reveal new insights into the regulation of lipid homeostasis.

-RNAi screen for negative regulators of dS6K-dependent-growth

The mechanisms that regulate the growth and size of different organs and tissues during the course of development are among the least known biological processes [START_REF] Leevers | Controlling the size of organs and organisms[END_REF]. The Drosophila wing serves as a good model system to analyze growth regulation [START_REF] Martin | Compartments and the control of growth in the Drosophila wing imaginal disc[END_REF]. It is formed by the cells of the wing imaginal disc, which initially contains about 40 cells [START_REF] Lawrence | The early development of mesothoracic compartments in Drosophila. An analysis of cell lineage and fate mapping and an assessment of methods[END_REF] that proliferate during the larval period to reach about 50,000 cells. There are about 10-11 divisions in the progeny of the initial cell, each of them takes about 10 hours [START_REF] Garcia-Bellido | Parameters of the wing imaginal disc development of Drosophila melanogaster[END_REF]. Shortly after the beginning of pupariation, cell divisions cease and the differentiation of the adult structures begins. The disc is subdivided during embryogenesis into anterior (A) and posterior (P) compartments which appear to grow independently [START_REF] Lawrence | The early development of mesothoracic compartments in Drosophila. An analysis of cell lineage and fate mapping and an assessment of methods[END_REF] and during second instar larval stage into dorsal (D) and ventral compartments (V) (Diaz-Benjumea and Cohen, 1993). The TOR (target of rapamycin) has been revealed as an effector of an insulin-and nutrient signaling pathway which regulates growth as well as metabolism. As stated earlier that the key downstream effector of insulin-and nutrient-mediated dTOR (Drosophila target of rapamycin) is dS6K (Drosophila ribosomal protein S6 kinase) which acts as a regulator of growth [START_REF] Montagne | Drosophila S6 kinase: a regulator of cell size[END_REF]. Using the ap-Gal4 driver, ectopic expression of dS6K within the dorsal compartment of the developing wing imaginal disc induced a moderate overgrowth and caused a bending down of the adult wing [START_REF] Montagne | Drosophila S6 kinase: a regulator of cell size[END_REF]. It has been described that the Drosophila PDK1 (dPDK1) when co-induced with dS6K in the dorsal compartment of the wing disc caused an enhancement of the wing bending-down phenotype, whereas expression of dPDK1 alone had no effect to this phenotype (Radimerski et al., 2002). It has been also described that the expression of a particular phosphorylation-site mutants of dS6K caused an enhancement or suppression of the wing phenotype depending upon the increase or decrease in the activation state of dS6K, respectively [START_REF] Barcelo | Altering Drosophila S6 kinase activity is consistent with a role for S6 kinase in growth[END_REF]. These findings demonstrated that the wing bending-down phenotype varies according to the dS6K activity. Thus, this sensitized phenotype was used in a gain-of-function genetic strategy to identify novel positive regulators of dS6K-dependent growth. During the screen, approximately 5,000

Enhancer-Promoter (EP) lines were co-induced with dS6K in the developing dorsal wing compartment. Since several enhancers, but too many suppressors were found, this study revealed that the bent-down wing phenotype could be efficiently used for enhancement, but not for suppression. In this screening for new positive regulators of dS6K-dependent growth, the Drosophila hormone receptor 3 (DHR3) was found to be one of the strongest enhancers [START_REF] Montagne | The nuclear receptor DHR3 modulates dS6 kinase-dependent growth in Drosophila[END_REF].

It is to be worth noting that expression of dS6K in other tissues did not produce any significant phenotype [START_REF] Montagne | Drosophila S6 kinase: a regulator of cell size[END_REF]. Therefore, larval wing imaginal disc which gives rise to future wing, proved to be a valuable and suitable tool for the search of growth regulators interacting with dS6K. Taking advantage from the UAS-RNAi lines from NIG, we started a project in order to find out novel regulators of dS6K signaling.

We have exploited the sensitized phenotype, such that ectopic expression of dS6K within the developing dorsal compartment causes the wing to bend-down. Using this sensitized phenotype we performed a genome-wide RNAi screening to identify novel potential regulators, which negatively regulate dS6K-dependent growth.

-A protein-protein interaction network through RNAi

The genetic approaches mainly focus on generating data on a gene-by-gene basis and creating a protein-protein interaction network which then prove to be a valuable information data. Hence, genetic screens, either for specific phenotypes or for modifiers of gene function, are thus a valuable source of large-scale interaction data [START_REF] Aerts | Integrating computational biology and forward genetics in Drosophila[END_REF]. But the high quality functional annotations through computational predictions of gene function alone remain much higher than genetic screening data [START_REF] Adie | SUSPECTS: enabling fast and effective prioritization of positional candidates[END_REF].

However, protein interaction networks summarize large amounts of protein-protein interaction data, both from individual, small scale experiments and from automated highthroughput screens. Sometimes, it seems that genetic interactions in model organisms constitute a potentially invaluable source of in vivo interaction data for systems biology provided that throughput and speed can be increased. For example, the BioGRID database currently contains approximately 53,000 genetic interactions compared to almost 100,000 physical interactions [START_REF] Stark | BioGRID: a general repository for interaction datasets[END_REF]. On the experimental side, the first direct large-scale protein interaction data were presented in 2000 [START_REF] Ito | Exploring the protein interactome using comprehensive two-hybrid projects[END_REF];

both studies used yeast two-hybrid technology. Two years later, the first large-scale protein complex purification data sets were published.

The recently developed RNA interference (RNAi) technology has created an unprecedented opportunity which allows the function of individual genes in whole organisms or cell lines to be interrogated at genome-wide scale. Therefore, a genomewide or even large-scale screening approach and side by side the computational predictions and/or annotations of gene-gene or gene-pathway associations, simultaneously both are biologically meaningful [START_REF] Aerts | Integrating computational biology and forward genetics in Drosophila[END_REF]. However, multiple issues, such as off-target effects or low efficacies in knocking down certain genes, have produced RNAi screening results that are often noisy and that potentially yield both high rates of false positives and false negatives [START_REF] Wang | A network-based integrative approach to prioritize reliable hits from multiple genome-wide RNAi screens in Drosophila[END_REF]. By analyzing 24 genomewide RNAi screens interrogating various biological processes in Drosophila, Wang et al found that RNAi positive hits were significantly more connected to each other when analyzed within a protein-protein interaction network. Based on this finding, Wang et al developed a network-based approach to identify false positives (FPs) and false negatives (FNs) in these screening results. Using two genome-wide RNAi screens and integrating these screening results with protein-protein interaction (PPI) network extensively validated this approach from multiple aspects [START_REF] Wang | A network-based integrative approach to prioritize reliable hits from multiple genome-wide RNAi screens in Drosophila[END_REF].

Keeping in view the practical application of the genome-wide RNAi screening data we have also generated a protein-protein interaction network using different web based sources. An interaction network of all the candidate genes have been presented in the manuscript which is, in fact centrally based on dS6K-depensent growth.

larval/pupal phenotype and could easily be analysed for the phenotype of developmental arrest and hence, looking for candidates potentially involved in the process of steroidogenesis.

-RNAi screen for regulators of lipid metabolism at the fat body

Initially, for the screening at fat body; 4-5 UAS-RNAi males were crossed to seven virgin females of ppl-Gal4/UAS-LSD2-GFP at 25°C, and let these flies laying eggs for 2 days. Five to six days AED (after egg deposition), third instar feeding larvae were collected and transferred to small petri-dishes with fresh food and let them feeding for at least 4 hrs. under optimal conditions. Full fed third instar larvae were then dissected and the fat body was collected quickly on slides for microscopic analysis using both normal and fluorescent light.

For "flip-out" clones [START_REF] Struhl | Organizing activity of wingless protein in Drosophila[END_REF] in the larvae by using heat shock promoter (hsflp), the UAS-RNAi males were crossed with flip-out flies and 24-48 hrs.

later, the tubes were heat shocked for 12 minutes at 37°C. After 5-6 days of egg laying, the third instar larvae were transferred to new food media in petri-dishes for feeding of 4

hrs. The fat body was then dissected in saline buffer and analysed under microscope for lipid droplet phenotype using fluorescent light. The selected candidate genes were further analyzed through a starvation of overnight. For this purpose, the third instar larvae were put into 10% sucrose solution for overnight (at least 12 hrs.) and then dissected for phenotypic analysis of lipid droplets.

-Lipid droplet staining

Nile red was reported as a selective fluorescent stain for the detection of intracellular lipid droplets by fluorescent microscopy [START_REF] Greenspan | Mevinolinic acid biosynthesis by Aspergillus terreus and its relationship to fatty acid biosynthesis[END_REF]. Dissection of the fat body was carried-out in PBS (immediately collected in microtiter-plate on ice (PBS), the GFP discs were protected from light as much as possible). Fixation was performed in 20 % paraformaldehyde (PFA) (in PBS) at Room Temp. for 20 min. After removing the fixation solution, Nile red was added to the secondary antibodies solution in dilution 1:1000 [START_REF] Bulankina | TIP47 is recruited to lipid droplets and important for the organelle biogenesis and function[END_REF]. To visualize the nuclei, DAPI (0.2%) was also added. After, the fat body was washed with PBT as well as PBS respectively. Then pipetting with the cut-end tip, the fat body was mounted on slides by using citiflour and used quickly for phenotypic analysis or stored at 4°C.

Oil Red O (ORO) staining was also performed as a visual marker to evaluate the lipid droplet contents of the fat body cells. Dissection of the fat body was carried-out in PBS (1X) and fixation was done in paraformaldehyde (4% in PBS) in PBS at Room Temp. for 10 min. After removing the fixation solution, the specimens were rinsed twice with distilled water, incubated for 20 to 30 min in Oil Red O stain (6 ml of 0.1% Oil Red O in isopropanol and 4 ml distilled water: prepared fresh and passed through a 0.45-mm syringe filter) [START_REF] Gutierrez | Specialized hepatocyte-like cells regulate Drosophila lipid metabolism[END_REF]. Stained fat body was mounted in citiflour and scored for lipid droplet phenotype quickly or sotred at 4°C.

-RNAi screening for negative regulators of dS6K-dependentgrowth

To screen for negative regulators of dS6K-dependent growth, 6-7 virgins of ap-Gal4>UAS-dS6K line were crossed with 4-5 UAS-RNAi males. All the crosses were done at 25°C. The selected candidate UAS-RNAi lines were re-crossed with ap-Gal4>UAS-dS6K and ap-Gal4 flies both at 25°C and 29°C. Notably, the UAS-RNAi males were crossed to ap-Gal4 line to eliminate an RNAi line which itself gives an effect. The analysis of the wing phenotype of all the crosses of UAS-RANi lines were made very carefully by using normal microscope (Leica MZ 6).

-Fly strains used during the study

The following alleles or transgenic lines were used: W 1118 , ap-Gal4>UAS-dS6K/CyoGal80, ap-Gal4/CyoGal80, UAS-dS6K/SM5-TM6B, ago 1 FRT80B/TM6B, ago 3 FRT80B/TM6B, ago 1 Df(3L)H99,FRT80B/TM6B, ago 1 hid 05014 , FRT80B/TM6B, ey-Gal4, Da-Gal, act-Gal4, ppl-Gal4, ppl-Gal4>LSD2-GFP, eyFLP;ey-Gal4;"M"-FRT80B/SM5-TM6B, hs-flp;α-tub>FRT-stop-FRT>UAS-GAL4-UAS-GFP/SM5- For RNAi screening, UAS-RNAi lines were used from NIG Japan; and for further experiments, RNAi lines were ordered from VDRC. Flies were balanced using double balancer yw; Sp; MKRS/SM5-TM6B for subsequent crosses.

-Immunostaining

Dissection of the discs/tissues was carried-out in PBS (immediately collected in microtiter-plate on ice (PBS), the GFP discs were protected from light as much as possible). Fixation was performed in 4% Formaldehyde in PBS at room temp. (RT) for 30 min. After washing of discs with PBT at least 3 times for about 10 min each, permeabilisation and blocking for 40 min at RT with PTF were done. Following immediately, incubation with PTF diluted primary antibody was carried out for overnight at 4°C. Then the discs/tissues were washed 2-times with PBT for 5-10 min, following 1wash for 5 min at RT with PTF and 1-wash for 5-10 min at RT with PBT were carried out. Then incubation with secondary was performed for 2 hrs at RT (0.1% PTF + 2/100 DAPI + 1/500 Ab). TOPRO-3 (diluted in DABCO) was also used to stain the nucleic acid. The tissues/discs were washed 3-times with PBT and at the last kept in PBS at RT.

The mounting was done in citiflour and kept at 4°C for microscopic analysis.

The following primary antibodies were used: anti-dS6K (rabbit, 1:50) ({Montagne, 1999 #8}), Anti-dS6K P (Cell Signaling Technology®), anti-βgal (rabbit, 1:200, DSHB), anti-GFP (mouse, 1:500, Roche Cat: 11814460001) and anti-Gal-4 (mouse, 1:200; Abcam).

Secondary Antibodies included Alexa 488 (anti-rabbit, Invitrogen: A-11078), Alexa 488

(goat anti-mouse, Invitrogen: A-21121), Alexa 568 (goat anti-rabbit, Invitrogen Lot:

633962A) and Alexa 594 (anti-rabbit, Molecular Probes: A-21441).

-Eye/thorax measurement:

Eyes and thorax of flies were photographed with a microscopic attached camera (Leica MICRO MECANIQUE), and then were redrawn with Adobe Photoshop. The pixel sizes were counted for eye and thorax separately for each fly by CoralDraw Graphics Suit 12.

Males and females were photographed and measured separately.

-Microscopy

The lipid droplet phenotype was always analyzed using "objective" of 40X magnification with a drop of Oil (Zeiss Immersol 518F) at camera-fitted Leica flourescence microscope (Leitz DMRD). The images were imported into Adobe Photoshop, assembled and converted to merged ones. For confocal microscopy (Nikon;

Eclipse TE2000U), preferably an objective of 60X magnification was used with a drop of oil (Zeiss Immersol 518F). But in some cases, to better visualize the slides, an objective of 100X magnification was also used with a drop of oil. An average of at least 3 images, along with 1 µm size of each stack was performed. The images were imported into ImageJ and Adobe Photoshop, then assembled and converted to merge images accordingly.

-Protein-protein interaction network data analysis

To generate a protein-protein interaction signaling network, two main websites "BioGRID 3.0 " (Biological General Repository for Interaction Datasets) and "BOND OA " (Biomolecular Object Network databank) were used (http://thebiogrid.org/; http://bond.unleashedinformatics.com/). Both of these websites contain a huge proteinprotein interaction database based on yeast-two-hybrid assay. There are different enhancer/suppression interactions of the proteins which exist at these websites. For the reconstruction the dS6K-based growth networking pathway, only the physical interactions of the selected candidate genes were scored.

-Quantitative PCR (q-PCR)

For RNA extraction, twenty 20 adult flies were collected in a 1.5-ml eppendorf tube (with lid) with 100 μl beads and placed at -20°C. After thawing on ice, 500 μl of TRIZOL® Reagent was added to each tube and homogenized for 30 s by Precellys 24.

The samples were incubated at room temperature for 5 min., then 100 μl of chloroform was added and vortexed for 2 × 30 s. The samples were centrifuged at 12,000 × g for 15 min at 4°C. The supernatant was collected (250 μl, aqueous phase) in a clean tube. Then 250 μl of isopropanol (stored at room temperature) was added to precipitate RNA and mixed gently by inverting the tube three times. The samples were then centrifuged immediately at 12,000 × g for 15 min at 4°C. The supernatant was removed by inverting the tube and the RNA pellet was washed with 400 μl 70% ethanol (room-temperature) and centrifuged at 12,000 × g for 10 min at 4°C. The supernatant was poured without disturbing the pellet, and then the pallet was air-dried for about 10 min. and finally redissolved with 50 μl of water. The RNA concentration was determined using UV spectrophotometry [START_REF] Romeo | Drosophila immunity: methods for monitoring the activity of Toll and Imd signaling pathways[END_REF].

For cDNA synthesis, filter tips were used to prevent contamination of the samples by foreign amplicons. The reverse transcription was performed in a 20 μl final volume. For each experiment, two control samples were included: one without RNA and the other without Superscript-II (SSII-RT; Invitrogen). The followed protocol was: In a clean 0.2-ml PCR tube add: RNA (500 ng/μl) 2 μl, Random hexamers (50 ng/μl) 2 μl, dNTPs mix (10 mM) 1 μl, H2O 7.5 μl, denature samples at 65°C for 10 min., Quick-chill on ice and centrifuge briefly. In each tube add: 5× SSII-RT buffer 4 μl, Dithiothreitol (DTT) (0.1 M) 2 μl, RNaseOUT™ RRI 0.5 μl, SSII-RT 0.5 μl, Place the tubes in a thermal cycler (heated lid at 70°C) and run the following: Incubate at 42°C for 50 min., Denature at 60°C for 15 min. Dilute RT products to 1/20 in water and tore diluted RT products at -20°C [START_REF] Romeo | Drosophila immunity: methods for monitoring the activity of Toll and Imd signaling pathways[END_REF].

For RT-PCR and gene expression quantification, the experiment was performed in 10-μl plates using the LightCycler® 480 System. A control sample containing water was included instead of RT product. The following PCR master mix was prepared for each sample: Light cycler® 480 SYBR Green I master 5 μl, forward primer 100 μM 0.125 μl, reverse primer 100 μM 0.125 μl, PCR-grade H 2 O 2.5 μl. In each plate -96 PCR master mix 8 μl and diluted RT product 2 μl was added. The following program was followed using the LightCycler® 480SW 1.5: denaturation: 95°C for 10 min., Cycling repeated 40 times: 95°C for 10 s, 60°C for 15 s, 72°C for 20 s, melting curve: 95°C for 5 s, 60°C for 5 s, 72°C for 15 s, 70°C→95°C with 0.1°C/s ramping ( [START_REF] Romeo | Drosophila immunity: methods for monitoring the activity of Toll and Imd signaling pathways[END_REF]).

The absolute quantification was monitored using the LightCycler® software and the gene expression level (R) was calculated as described by Romeo and Lemaitre [START_REF] Romeo | Drosophila immunity: methods for monitoring the activity of Toll and Imd signaling pathways[END_REF].

-Western blotting

Protein extraction was performed as follows: L2 larvae of ago RNAi were collected and transferred to a fresh food and left feeding until they reached to L3. Full fed L3 larvae were collected in PBS and transferred to 1.5 ml eppendorf tubes. ago mutants are lethal at early larval stage, and we were able to find L2 transheterozygotes (ago 1 /ago 3 ), therefore L2 larvae wer collected for protein extraction. Batches of 50 larvae were prepared and immediately put into liquid nitrogen. The larvae could be stored in liquid nitrogen for weeks (Radimerski, 2002) Electrophoresis was conducted at 30 mA in an electrophoresis tank in 1X running buffer (5X stock: Tris base 15.1g (25mM), Glycerine 94g (250mM), SDS 50ml (10%), water 950ml). Proteins separated by SDS-PAGE were then transferred onto nitrocellulose membrane for western blot analysis. Eight pieces of whatman paper (10"x10"cm) and one piece of nitrocellulose membrane were prepared. Four sheets of whatman paper immersed in the blot buffer (methanol 200ml, glycerine 2.93g, Tris base 5.81g, SDS 0.37g, water to make up vol. 1L) and placed in the chamber. The gel was also soaked in the blot buffer before placing on the paper. Then the membrane dipped in the blot buffer was placed on top of it and the air bubbles were removed. Another whatman layer of four sheets was dipped in the buffer and placed on top of the gel. A glass pipette was rolled on top of the stack to remove air bubbles. The proteins were transferred onto the membrane using 10mA current for 90 min. After the transfer, membrane was briefly washed with PBS, incubated in PBST (PBS 1X, 0.5% Tween) containing 5% milk powder for 1 hour at room temperature to block all non-specific interaction sites on the membrane. After blocking, the primary antibodies diluted in PBST, were added onto the membrane and incubated overnight in the cold room (constantly vortexing at low speed).

The non-specifically bound antibody was washed off by incubating the blot with PBST, changing the buffer once every 10 min for three times. The membrane was then subsequently incubated with the respective secondary antibody coupled to horseradish peroxidase for 1 h at room temperature and washed again 5 times for 5 min. The nitrocellulose membrane was incubated at room temperature for 1 min in enhancer and peroxide solutions mixed in a 1:1 ratio (enhanced chemoluminescent kit; PIERCE). The membrane was wrapped in a polythene sheet and signals were detected accordingly.

-Drosophila cell culture

RNAi assays in S2 cells were performed in the following way. Double stranded RNA (dsRNA) molecules were synthesized by in vitro transcription from PCR generated templates having a T7 promoter attached to both ends (T7 MEGAscript Kit (Ambion)).

The length of the RNA molecules ranged to 500 base pairs. We followed the protocol of Ramadan et al. during the whole experiment (for detail see [START_REF] Ramadan | Design and implementation of high-throughput RNAi screens in cultured Drosophila cells[END_REF]. We used exponentially growing S2 cells in Schneider"s Drosophila medium (Sigma) with serum and placed 500µl of them in 12-well plates at the final concentration of 4x10 5 cells/ml along with 4 μg of dsRNA per well. Then, after 3 days of incubation; 500 μl of new medium was added with serum and without serum concomitant with 4 μg of dsRNA per well. The cells were serum starved for one day. At the 4 th day, cells were harvested and washed with PBS 1X. The cells were lysed for protein extraction (Radimerski et al., 2002a). Protein concentration was estimated using a spectrophotometer and the Bradford assay with a kit from BioRad. The proteins were resolved through SDS-PAGE and transferred to a nitrocellulose membrane. Western blot assay was performed using antibodies against dS6K, P-dS6K and secondary anti-rabbit IgG, separately (see above).

After reaction using enhanced chemoluminescent kit (PIERCE), the signals were detected by LAS3000 (Fugifilm apparatus) and processed accordingly.

Chapter 3

Chapter 3 -Results

3.1-Project goals

The cellular growth in response to nutrition is centred on TOR kinase, which in turn modulates protein translation machinery, and ribosome biogenesis. With an increase in growth, there is increasing demand of energy which is produced through metabolism.

Finally, the Drosophila TOR signaling pathway also controls the developmental timing through the regulation of the synthesis of steroid hormone ecdysone, which controls larval developmental steps. Therefore, the whole research activities of our group are linked to growth, development and entangle also to the metabolism. To better understand the mechanisms of regulation of these three processes in Drosophila, we took advantage of the availability of RNAi collections of the National institute of Genetics (NIG, Japan), and embarked on a saturating screening project. Three different strategies were designed:

1. RNAi screening for novel regulators of steroidogenesis 2. RNAi screening for regulators implicated in lipid metabolism

RNAi screening for negative regulators of dS6K-dependent growth

Although I was also involved in the preparative steps of the first screen, but my PhD work mainly concerned with the two latter screens. All these three screens are directly or partially linked to the TOR signaling pathway.

When the larvae have reached a minimum viable weight, they possess sufficient nutritional stores to undergo metamorphosis. The transition from feeding and growth to pupariation at the onset of metamorphosis is triggered by the production of a peak of ecdysone in the prothoracic gland (PG). The TOR kinase has been proposed to constitute the physiological node between nutritional status and ecdysone production, since its inhibition through over-expression of the tumor suppressor TSC1/TSC2 in the PG causes a delay in developmental timing and organismal overgrowth [START_REF] Layalle | The TOR pathway couples nutrition and developmental timing in Drosophila[END_REF]. In order to explore the systemic control of developmental steps mediated by ecdysone, our RNAi screen ( 1) is aimed to find-out novel regulators of steroidogenesis in Drosophila.

Microarrays approaches recently showed that the TOR signaling pathway regulates the expression of genes encoding enzymes of lipid metabolism [START_REF] Li | Nutritional control of gene expression in Drosophila larvae via TOR, Myc and a novel cis-regulatory element[END_REF].

Consistently, metabolic studies in Drosophila revealed that the TOR kinase directly controls lipid metabolism, as its down-regulation induces an increase of the size of the lipid droplets (LDs) in the fat body, a phenotype that is also observed following starvation [START_REF] Colombani | A nutrient sensor mechanism controls Drosophila growth[END_REF]. Therefore, in another screen (2), we focused our attention to recapitulate the starvation induced lipid droplet phenotype using the UAS-RNAi lines to find out novel regulators of lipid metabolism.

A well-known direct target of TOR is the S6 kinase, whose Drosophila homologue (dS6K) has been described to be a regulator of size gauging [START_REF] Montagne | Drosophila S6 kinase: a regulator of cell size[END_REF]. A genetic screen for positive regulators of dS6K has been successfully performed in our laboratory using a sensitized dS6K-dependent growth, leading to the identification of a novel positive regulator of growth [START_REF] Montagne | The nuclear receptor DHR3 modulates dS6 kinase-dependent growth in Drosophila[END_REF]. Our genome-wide screen

(3) to identify novel negative regulators of dS6K-dependent growth followed the same strategy using the RNAi collection.

In the following sections, first I would like to describe our general screening plans in order to compare these in discussion; and the percentage of positive hits which has

-RNAi screening for novel regulators of steroidogenesis

In Drosophila, nutrition modifies the timing of development by acting on the production of the ecdysone at the PG, a process that is in part controlled by the neurohormone PTTH (Prothoracicotropic hormone) [START_REF] Gilbert | Control and biochemical nature of the ecdysteroidogenic pathway[END_REF], which may regulate TOR signaling [START_REF] Layalle | The TOR pathway couples nutrition and developmental timing in Drosophila[END_REF]. Ecdysone signaling actually triggers a cascade of nuclear receptors and we recently observed that specific RNAis to these receptors also impede the progression of the developmental steps, a phenotype that could be suppressed by feeding ecdysone (Parvy et al, unpublished results). Therefore, we performed a genome-wide RNAi screen to find-out new regulators participating in the production or secretion of Ecdysone and ultimately regulating the steroidogenesis in drosophila. The transgenic RNAi lines were expressed in the steroidogenic cells of the ring gland using phm-Gal driver. However, as the phenotype had been followed during larval development, the RNAi transgenes inserted on second or third chromosomes were first balanced over the combined SM5-TM6B balancers. My contribution to this screen concerns this preparative steps, since SM5-TM6B balanced males were also used in the screen for LD size described below. Nonetheless, I briefly describe this; also I will present a comparative analysis for screening procedures in the discussion section. Following the crosses with the phm-Gal4 driver, Tubby + larvae, which correspond to the bone fide animals to score, were carefully analyzed. A total of about 11,000 inducible RNAi lines were screened, predicted to approximately 7,000 genes corresponding to roughly half of the genome of Drosophila melanogaster. This way, more than 620 candidate genes were retained, as their RNAi induced a phenotype ranging from early developmental arrest to extension of growth period and eclosion of giant adults. All these candidates were then classified into 15-different groups regarding their annotated functions at flybase (Fig. 19A), or into 9-different phenotypic groups regarding the stage of developmental arrest (Fig. 19B). Identification of RNAi to genes previously described to regulate steroidogenesis were also found in the screen, i.e. ras [START_REF] Caldwell | Ras activity in the Drosophila prothoracic gland regulates body size and developmental rate via ecdysone release[END_REF], which makes a confirmation to the efficiency of the screen for potential candidates regulating steroidogenesis. Beside a high number of unknown genes, the selected candidates fall into several functional groups which govern basal cellular activity: metabolism, signaling, chromatin structure, transcription, translation and protein maturation. A few of these candidates concerned with steroidogenesis or intracellular trafficking are rather directly linked to ecdysone production or secretion, as the process of synthesis takes place in various sub-cellular compartments. Neither the significance of the observed phenotypes, nor the function of the selected candidates will be further discussed, since it was not the focus of my PhD work. The comparative analysis for screening procedures will mostly concern the number of candidate genes identified, regarding the screening strategy.

-RNAi screening for new regulators of lipid metabolism

When drosophila larvae are fully fed, they accumulate lipids in fat body cells [START_REF] Britton | Drosophila's insulin/PI3-kinase pathway coordinates cellular metabolism with nutritional conditions[END_REF][START_REF] Scott | Role and regulation of starvation-induced autophagy in the Drosophila fat body[END_REF]. Under starvation conditions, the stores of lipids in the fat body cells are remobilized, which results in an increase of the size of LDs [START_REF] Juhasz | The Drosophila homolog of Aut1 is essential for autophagy and development[END_REF][START_REF] Rusten | Programmed autophagy in the Drosophila fat body is induced by ecdysone through regulation of the PI3K pathway[END_REF][START_REF] Gutierrez | Specialized hepatocyte-like cells regulate Drosophila lipid metabolism[END_REF]. Interestingly modulation of a number of growth or metabolic regulators phenocopies the starvation induced phenotype in the fat body [START_REF] Colombani | A nutrient sensor mechanism controls Drosophila growth[END_REF][START_REF] Hietakangas | Regulation of tissue growth through nutrient sensing[END_REF][START_REF] Britton | Drosophila's insulin/PI3-kinase pathway coordinates cellular metabolism with nutritional conditions[END_REF][START_REF] Beller | COPI complex is a regulator of lipid homeostasis[END_REF]. Therefore, taking advantage of the UAS-RNAi library from NIG, we screened these lines to identify novel regulators of lipid storage and metabolism at the fat body of 3 rd instar larvae.

As for the screen for ecdysone production at the PG described above, the screened phenotype was monitored in larvae and thus the UAS-RNAi inserted on the second or the third chromosomes were first balanced in front of the combined SM5-TM6B balancers (Fig. 18). Initially, the RNAi transgenic lines were expressed within the entire larval fat body using the ppl-Gal4 driver, combined to an UAS-LSD2-GFP transgene. Because the LSD2-GFP fusion protein surrounds the LDs, the green fluorescence served as a read-out to score the characteristic LD phenotype (Fig. 20).

Fat body of third instar larvae were dissected and directly analyzed under light and fluorescent microscopes. We analyzed the phenotype of LDs both under full fed as well as under starvation conditions. This way, using the ppl-Gal4 driver line, we selected candidate genes, which displayed various types of phenotypes, i.e., small, large, medium sized LDs, scattered, oblong and spherical shaped LDs (data not shown). However, it was laborious and time consuming as we always needed an external control for each set of dissection, while the number of selected UAS-RNAi lines were also increasing. Approximately, seventy five UAS-RNAi lines were selected out of total of four hundred fifty lines, the phenotype of which finally appeared to be poorly relevant (data not shown).

Considering that too many genes were selected using the ppl-Gal4 driver, we switched to the flip-out strategy. In this setting, a silent Gal4 transgene is induced by an Flp/FRT-dependent recombination to generate random marked clones that expressed UAS-GFP and UAS-RNAi together. Clones within the fat body, were obtained by a 37°C heat-shock treatment to induce the Flp recombinase in 24-48 hrs old larvae (α-tub>FRT-w + STOP-FRT>Gal4, UAS-GFP) and analyzed 3 days later. This process induces recombination between the FRT sites and removes the w + STOP cassette in clones, thus allowing the expression of Gal-4 to activate the UAS-GFP and the UAS-RNAi transgenes simultaneously. The technique was advantageous, since the LDs of the UAS-RNAi expressing clones (GFP positive cells) could be directly compared to the LDs of the neighboring cells as internal control (GFP negative cells). Moreover, we performed a pilot experiment using a transgenic RNAi line to Rheb (Ras homolog enriched in brain) to check the feasibility, and to improve the specificity of our screening. Consistent with its function as an activator of the TOR/S6K signaling cassette [START_REF] Saucedo | Rheb promotes cell growth as a component of the insulin/TOR signaling network[END_REF][START_REF] Stocker | Rheb is an essential regulator of S6K in controlling cell growth in Drosophila[END_REF], the clonal expression of Rheb-RNAi provoked a dramatic increase in the size of LDs coupled to a decrease in LDs number (Fig. 21). In nutshell, the flip-out technique was found to be more efficient and faster to screen for regulator of lipid metabolism and of the TOR/S6K pathway as compared to the strategy using the ppl-Gal4 driver. Our pilot experiment through the flip-out technique gave a break-through and a sound basis to our screening scheme; therefore, we also re-screened the already selected candidates using ppl-Gal4 line.

Altogether, a total of 7,300 lines roughly corresponding to 4,000 distinct genes were screened-out and scored only for an increase in LD size. This way, twenty four (24) candidate lines were found to slightly modulate the LD phenotype (Fig. 22; Table 2).

The selected lines were further analyzed through Nile Red or Oil Red O (ORO) staining to visualize the lipid droplets phenotypes in more detail, but did not further improve the selection (data not shown). For most of the RNAi lines retained, we observed a decrease of number of LDs concurrent to the increase in LD size of clonal cells, as compared to the surrounding control wild-type fat body cells. Few lines produced smaller sized LDs phenotypes and an increased number of LDs per cell (data not shown). Since the Vienna Drosophila Research Center (VDRC) also provides transgenic RNAi collection, the more significant candidates were further tested using this library. A major issue of the RNAi usage is the existence of "off-targets", namely alternative genes, which may be affected by the RNAi. Considering that the template to produce the precursor dsRNA derived from a few hundred nucleotide genomic sequence, this precursor can potentially produce many small interfering RNAs (siRNAs); some of these may affect an unexpected gene. The transgenic RNAi lines of VDRC were generated later and regarding the issue of off-targets, were designed to have much less potential unrelated hits. Therefore, we further analyzed our selected candidate lines using RNAi collection of VDRC.

This screen for modulator of LD size led to the identification of various candidate genes (Table 2): Eight of these encode enzymes of lipid metabolism (sxe2, thiolase, start1, CG3961, CG5122, CG4630, cyp4g and α/βhydrolase), three encode ribosomal proteins (mRpS31, mRpS35 and mRpL28), four encode potential signaling molecules (Pak3, twins, CG2165 and Rack1) and four others encoded proteins involved in neurological activity (Fasciclin2, period, dunce and RhoGAP93B). Regarding the latter group, one candidate gene, Fasciclin2 gene (Fas2) was found to produce a slightly larger sized lipid droplet phenotype (Fig. 23). Since three Fasciclin genes (Fas1, Fas2

and Fas3) have been described in Drosophila, RNAi lines from VDRC that affect specifically each of these genes were tested. The fat body of third instar larvae expressing flip-out clones to each of these RNAi line was dissected and carefully analyzed for the phenotype of LDs both under full fed as well as starvation conditions.

Strikingly, we did not find a larger sized lipid droplet phenotype compared to larger size of lipid droplets with RNAi to Rheb. However, an RNAi line to Fas3 (CG5803)

was found to produce a moderate increase of LD size, coupled to a decrease in LDs number (Fig. 23). These findings suggest that one of the Fasciclin genes might affect lipid metabolism to control the size of LDs. However, three other candidate genes affecting LDs size (dunce, period and RhoGAP93B) have been previously described to act on neuronal activity support the notion that some genes are involved in both processes of nervous system and lipid metabolism. Another gene, Pak3 (CG14895)

showed a slight increase in the phenotype of LDs compared to controls (data not shown). Pak3 has been annotated to have a serine/threonine kinase activity acting as a signaling molecule. In addition, Pak3 had also been found through an RNAi screen to cause an axon guidance defect and hence might be involved in the development of the nervous system [START_REF] Winter | Repression of Dpp targets in the Drosophila wing by Brinker[END_REF]. However, we tested an RNAi to Pak3 from VDRC for further analysis. A subtle modulation in LD phenotype was observed (data not shown), which need to be further analyzed.

It is worth noting that, none of the selected candidate lines did produce an LD phenotype as severe as the one induced by Rheb RNAi (Fig. 21). Moreover, an RNAi screen in Drosophila S2 cells has been published by Guo et al. during the course of our screen. In this study, they searched for any kind of modulation in the LD phenotype and categorized these LD phenotypes into five distinct groups, and showed that about 1.5% of all the genes are involved in lipid-droplet formation and regulation [START_REF] Guo | Functional genomic screen reveals genes involved in lipid-droplet formation and utilization[END_REF]. We, therefore, did not further continue screening at the fat body and concentrated on the other screen (see below in the corresponding section).

-RNAi screening for negative regulators of dS6K-dependentgrowth

An ectopic expression of dS6K within the developing dorsal wing compartment using the apterous-Gal4 (ap-Gal4) driver induces a moderate overgrowth of this compartment and lead to a bending-down phenotype of the adult wing [START_REF] Montagne | Drosophila S6 kinase: a regulator of cell size[END_REF]. It has been discussed earlier that this bend-down phenotype of the adult wing varies according to the activation status of dS6K mutants [START_REF] Barcelo | Altering Drosophila S6 kinase activity is consistent with a role for S6 kinase in growth[END_REF] and can be modulated by the Drosophila PDK1 (dPDK1) (Radimerski et al., 2002). This sensitized genetic phenotype has been retained by our research group to search for novel modulators of dS6K-dependent growth. Following a gain-of-function strategy, a collection of approximately 5000 Enhancer-Promoter (EP)-lines were coexpressed together with dS6K in the developing dorsal wing compartment. The offspring was then scored for enhancement or suppression of the bending-down of the adult wing. This study identified novel positive regulator of dS6K, including the nuclear receptor DHR3 (Drosophila hormone receptor 3), which was further shown to be a positive regulator of growth [START_REF] Montagne | The nuclear receptor DHR3 modulates dS6 kinase-dependent growth in Drosophila[END_REF]. Moreover, it was observed in this study that the enhancement of the bent-down wing phenotype is a rare event and for most of the cases resulted from a specific interaction, whereas, suppression of this phenotype is very frequent and most likely reflects a non-specific interaction [START_REF] Montagne | The nuclear receptor DHR3 modulates dS6 kinase-dependent growth in Drosophila[END_REF]. Considering that the enhancement of the bending down results on cell growth increase and that the EP induction normally induces over-expression of the neighboring gene, this strategy may allow the identification of only positive regulators of dS6K-dependent growth.

Taking advantage of the UAS-RNAi library of NIG, we performed a loss-of-function genome-wide RNAi screen to further identify novel regulators of dS6K signaling.

Using the ap-Gal4 driver, each RNAi line was co-expressed with dS6K in the developing dorsal wing compartment and the adult wing was scored for enhancement or suppression of the bent-down wing phenotype. As expected, suppression appeared to be a frequent event (data not shown) and thus, was considered to be irrelevant, whereas, enhancement was observed with very low frequency (Fig. 24). Considering that an RNAi disrupts gene expression, and that enhancement of the wing phenotype reflects an increase of cellular growth, the candidate genes indentified in the screen are expected to act as negative regulators of dS6K-dependent growth. In the first step of the screening procedure, approximately 11,000 UAS-RNAi lines corresponding to roughly 7,000 genes were co-induced with dS6K in the developing dorsal compartment of the wing disc and scored for the enhancement of the dS6K-dependent wing phenotype. All the crosses were performed at 25°C and the RNAi lines which nonspecifically disrupt the wing development (i.e. Cyo-like, strong Cyo, flat, crumbled)

were discarded (data not shown). In this way, three hundred fifty seven of the UAS-RNAi lines were found to produce a strong bending-down of the adult wing. These lines were further analyzed for the effect on the wing when induced alone with ap-Gal4 driver. The UAS-RNAi which alone produced a strong bent-down of the adult wing was then discarded, since it was supposed to affect growth independent of dS6K.

For example, an RNAi to CG8931 when induced alone produced a bent-down wing phenotype that was almost the same as the phenotype produced in combination with dS6K (compare Fig. 24E and24E"). Moreover, one of the candidates was discarded (CG5580), as it has been described to control dorsal/ventral patterning of the developing wing and thus, most likely affects our expression system (data not shown).

The selected candidates were further analyzed using the corresponding RNAi line from the library of VDRC for confirmation of dS6K specific interaction (data not shown).

Moreover, to narrow the selection criteria, we also tested UAS-RNAi to the potential off-target genes of the selected RNAi lines. When co-expression of dS6K and an RNAi line to a potential off-target could reproduce the wing bend-down phenotype, the initially selected candidate was considered to be irrelevant and the corresponding RNAi line was discarded (data not shown). Following this additional selection step, several false positives were discarded. At higher temperature, the UAS-GAL4 system is known to be more efficient. Hence, to confirm the strong genetic interactions, the selected lines were then co-induced with dS6K both at 25°C and 29°C. This way, the selected UAS-RNAi lines were categorized into weak, mild and strong enhancers (Fig. 25). As depicted in the graph, 50 RNAi lines (0.45 %) produced a strong enhancement, 109 (0.99 %) produced a mild enhancement and 198 (1.8 %) produced a weak enhancement of the bent-down wing phenotype (Fig. 25). The selected 50 strong enhancer RNAi lines correspond to 45 different genes (Table 3), and out of these five genes showed an extreme bend-down phenotype when co-expressed with dS6K.

Finally, we focused only on the 45 candidate genes which produced a strong enhancement of the bent-down wing phenotype and were definitely confirmed through all the screening steps (Table 3). These selected candidates include eight genes encoding metabolic enzymes (CG4581, CG8798, CG3522, CG9165, CG3626, CG8349, CG6917, CG6947), three genes encoding enzymes with ubiquitin ligase activity (CG15010, CG5603, CG12799) and seven genes encoding signaling molecules (CG12015, CG8318, CG13850, CG1283, CG13229, CG5279, CG8985), the three latter encoding G-protein coupled receptors. Two selected candidate genes have not been annotated with any function at flybase, however, these genes encode for proteins with either F-box domain or WD-40 repeats (CG3909 and CG5961). These domains are particularly important for target protein interaction and then degradation.

Therefore, as for the gene encoding enzyme with ubiquitin ligase activity, the gene products might be involved in targeting a substrate to the proteasome to control its subsequent degradation.

As mentioned above, two UAS-RNAi lines for each single gene are normally available. For some genes, both RNAi lines were found as enhancers of the bent-down wing phenotype further validating our screening strategy. Those RNAi lines were specific to the CG15010, CG12015, CG1283, CG9165 and CG6917 genes (Table 3);

for each of them, none of the potential off-target genes tested were found to enhance the bent-down wing phenotype (data not shown), arguing that the corresponding gene products might be bona fide negative regulators of dS6K dependent growth.

Systematic studies aimed to establish a physical interactive inventory between the Drosophila gene products have been previously performed following the yeast-twohybrid strategy [START_REF] Giot | A protein interaction map of Drosophila melanogaster[END_REF]. Databases revealed that seven proteins (CG15010/Archipelago, CG8843/Sec5, CG8128, CG10622/Sucb, CG10295/Pak, CG14216 and CG12019/Cdc37) can physically interact with dS6K in yeast two-hybrid (Y2H) assay [START_REF] Formstecher | Protein interaction mapping: a Drosophila case study[END_REF]. Three of those were found through our RNAi screen, CG12019 (cdc37), CG15010 (ago) and CG8843 (sec5), although the former showed a mild-type enhancement of the bend-down phenotype. Since the NIG collection does not uncover the entire Drosophila genome, RNAi lines specific to the other dS6K physical interactors were ordered from VDRC and analyzed. In summary, out of the seven physical dS6K interactors, three were found to be potential negative regulators of dS6K-dependent growth (CG12019/Cdc37, CG15010/Ago and CG8843/Sec5). No strong enhancement of wing bend-down phenotype was observed with RNAi to the other four genes, neither at 25°C nor at 29°C, which exclude them as negative regulators of dS6K-dependent growth. Two genes CG8985 and CG13229 involved in G-protein coupled receptor signaling pathways, were found through our screen, but appeared to be off-targets of each other. This finding suggests that either several G-protein coupled receptors converge on dS6K signaling or that only one of them is specific for dS6K. It is to be worth noting that the RNAi to CG2165 and CG11081 (plexA) produced a strong enhancement of the bent-down wing phenotype (Table 3) and that the corresponding gene product for either of them has been described to physically interact with CG15010 (ago) in two-hybrid assay. Altogether, these data strongly argue for the specificity of our screening scheme.

Figure 24 -Modulation of the bend-down wing phenotype due to dS6K overexpression

The bending down of the adult wing due to dS6K overexpression in the dorsal compartment of the developing wing disc (A") is enhanced by co-induction of RNAi to CG1062 (B"), Thiolase (C") and plexin (D"). Induction of these RNAi lines alone (B, C and D), does not modify the wild type wing phenotype (A). The bending down phenotype is enhanced by an RNAi to CG8931 when co-induced with dS6K (E"). However induction of the CG8931 RNAi alone is sufficient to induce a bend-down phenotype (E). The selected ccandidate genes identified in the ap-screen are listed. The putative molecular and biological functions are taken from flybase [START_REF] Anonymous | The FlyBase Consortium). The FlyBase database of the Drosophila genome projects and community literature[END_REF].

Genes are indicated by their name or CG number. The asterisks indicate the RNAi lines, which produced an extreme enhancement of the wing bend-down phenotype.

in Fig. 27). Similarly CG18247 genetically interacts with CG2275, which itself interacts with EGFR, Ras85D, phyl and phl (not indicated in Fig. 27). These genetic interactions validate the relevance of this network. Interestingly, all the gene products of the network are linked together, so that any of the candidates can be physically associated with another one. These findings emphasize the role of various regulatory processes including signaling pathways, cell cycle control and protein degradation; and further suggest that these cellular processes interact together to coordinate the process of growth. The candidates selected in the screen are shown (blue circled) (for simplicity, the candidates for which no information is available, have been removed). The proteins that are reported to physically interact with dS6K (pink boxes), and the Tsc1/2 repressors of dS6K activity (green boxes) are indicated. Additional gene products necessary to establish the physical interactive network are represented (grey boxes). RNAi to some of these additional gene products have been tested in the screen and did not strongly enhance the bent-down wing phenotype (grey boxes with dark grey circles). Two genes, i.e. cact and CG4998 were found to be mild enhancers (grey circled, yellow boxes).

-Identification of upstream regulators of dS6K

In an attempt to discriminate whether a candidate gene is acting upstream or downstream of dS6K, we ubiquitously expressed the RNAi lines corresponding to the 45 strong enhancers retained in the screen. Larval extract were then prepared, scored and analyzed by Western-blotting with antibodies specific to either dS6K or to its active phosphorylated site (P-dS6K). All of these RNAi lines were viable in this genetic setting. However the effects on dS6K levels and/or phosphorylation varied a lot and were not reproducible in distinct experiments (data not shown). This variability may in part reflect the difficulty to precisely synchronise the larvae with respect to feeding and developmental stage. It may also be a consequence of the RNAi knock down that varies a lot depending on the experiment. In addition, we observed that ubiquitous expression of the RNAi did not produce lethality, whereas some mutant are described to be homozygous lethal, as previously reported for ago [START_REF] Moberg | Archipelago regulates Cyclin E levels in Drosophila and is mutated in human cancer cell lines[END_REF]. These different phenotypic effects suggest that induction of the RNAi lines in our genetic setting can never totally abolished the gene expression.

The Drosophila derived S2 cells have been described to provide an appropriate system to suppress an upstream regulator through direct application of specific double strand RNA (dsRNA) and then to monitor kinase activity or levels of a given intermediate of the insulin and nutrient signaling network [START_REF] Clemens | Use of double-stranded RNA interference in Drosophila cell lines to dissect signal transduction pathways[END_REF], Radimerski et al. 2002a[START_REF] Kim | Recombinant baculovirus-based multiple protein expression platform for Drosophila S2 cell culture[END_REF]. Therefore, we analyzed the expression and activity profiles of dS6K for few of the candidates. Five of the 45 candidate genes retained in the latter screen, i.e. CG15010, CG1283, CG8318, CG10139 and CG5279 (Table 3), were categorized as the most potent interactors, since co-induction of their specific RNAi with dS6K produced an extreme enhancement of the bent-down wing phenotype.

Specific dsRNA fragments to these 5 candidate genes and to 3 other candidates (CG1233, CG12819 and CG11081) were produced and incubated with cultured S2 cells. Cell extracts were then analyzed by Western-blotting using specific antibodies to either the full-length dS6K or to its phosphorylated T398 residue to evaluate dS6K levels or activity state, respectively (Fig. 28). To precisely assess the eventual variation of dS6K levels or activity, western-blotting was performed to α-Tubulin as internal

-Mammalian homologs of the most relevant dS6K modulators

The Drosophila gene CG10139 encodes a small protein of 224 amino acids that does not match to any mammalian counterpart. Protein blast search could identify homologues only in insects. No typical conserved domain is reported, except a potential pyrolysyl-tRNA synthetase domain with low homology with a bacterial enzyme. Neither phenotypic mutant analysis nor biological function is reported for this gene in Drosophila melanogaster [START_REF] Giot | A protein interaction map of Drosophila melanogaster[END_REF].

The gene CG8318 encodes a unique gene, which has been extensively investigated in

Drosophila, showing that it plays an important role in learning and memory [START_REF] Buchanan | A distinct set of Drosophila brain neurons required for NF1-dependent learning and memory[END_REF], although the initial characterisation revealed a role in the control of growth [START_REF] The | Rescue of Drosophila NF1 mutant phenotype by protein kinase A[END_REF]. Protein blast identifies a single mammalian homologue RasGTPase activating domain spanning amino acids 1247-1573 in drosophila and 1205-1551 in mouse (Fig. 29). Three distinct studies in mammals recently showed that NF1 also acts as a repressor of the mTOR/S6K pathway [START_REF] Johannessen | The NF1 tumor suppressor critically regulates TSC2 and mTOR[END_REF][START_REF] Dasgupta | Proteomic analysis reveals hyperactivation of the mammalian target of rapamycin pathway in neurofibromatosis 1-associated human and mouse brain tumors[END_REF][START_REF] Johannessen | TORC1 Is Essential for NF1-Associated Malignancies[END_REF], which firmly validates the specificity of our Drosophila-modifier screen to identify negative regulators of dS6K-dependent growth.

The gene CG1283 also called Cdep (Chondrocyte-derived ezrin-like domain containing protein) encodes a protein member of the FARP (Fibrinogen/angiopoietinrelated protein) family, for which 2 homologues have been found in mammalian species, Farp1 and Farp2; the latter exhibiting a higher degree of homology with Cdep (Fig. 30). Protein structure analysis reveals several conserved domains: the FERM (Farp ezrin moesin radixin) domain, found in cytoskeletal-associated proteins, a GEF (Guanine nucleotide exchange factor) domain for Rho and 2 PH (pleckstrin homology) domains (Fig. 30A). Little is reported in literature for proteins of the Farp family. A recent study in breast cancer reveals that Farp1 is a TGF-β target induced in the switch from cohesive to single cell motility. Therefore, Cdep is certainly one of the most interesting candidates found in our dS6K-modifier screen that should be further investigated

The gene CG15010 also known as archipelago (ago) has been extensively described in the introduction. Protein blast analysis identified a unique homolog in mammals, the FBW7 gene which is mutated in several human tumors. Sequence homology only concerns the C-terminal half, where the F-box and the seven WD repeats are located (Fig. 12 & Fig. 31). These proteins are reported to be a part of the E3-ubiquitine ligase complex that targets the substrate to proteasomal degradation. Consistently, our results

suggest that Ago may regulate dS6K degradation. Since nothing is known to date about the regulation of dS6K degradation, we then, decided to further investigate the potential interaction between Ago and dS6K. 3.9 -Archipelago is a negative regulator of dS6K levels 3.9.1 -Identification of Archipelago as a negative interactor of dS6K

Among the RNAi lines of 45 candidate genes which produced a strong interactions with dS6K (Table 3), we focused our attention on archipelago (ago). As mentioned in the introduction (section: 1.4.4.3) the protein Ago has been previously described to promote protein degradation of various growth regulators. Since dS6K is a positive regulator of cell growth [START_REF] Montagne | Drosophila S6 kinase: a regulator of cell size[END_REF] and nothing is known about its degradation, it was tempting to speculate that Ago may also control the degradation process of dS6K. In the screen, both UAS-RNAi lines to ago (ago RNAi R2 & ago RNAi R3) were found to be strong enhancers of the dS6K-dependent growth phenotype either at 25°C or 29°C (Fig. 32). Although a subtle bent-down of the wing could be observed when ago RNAi was induced alone with the ap-Gal4 driver (Fig. 32B,D,F,H), the strong bending observed when dS6K and ago RNAi were co-induced together support the notion that Ago may be a negative regulator of dS6K.

To ensure that the wing phenotype was specific to Ago and did not result from any non-specific effect, potential off-targets of ago RNAi were ordered from VDRC (CG16880, CG31132, CG4144, CG8108, CG15239, CG32464, CG3308, CG32685, CG6181, CG14107, CG12052 and CG9781). UAS-RNAi to these potential off-targets was co-induced with dS6K in the dorsal compartment of the developing wing using the ap-Gal4 driver both at 25°C and 29°C. Importantly, none of these RNAi enhanced the bent-down of the wing, which further argue for the specificity of Ago to regulate dS6K-dependent growth. To confirm that the ago RNAi disrupts ago expression levels, RT (Reverse Transcriptase) Quantitative-PCR was performed. When induced by either of the ubiquitous Da-Gal4 or Act-Gal4 drivers, both of the ago RNAi lines provoked a severe decrease of ago expression (Fig 33). Altogether, these findings strongly suggest that the dS6K/ago RNAi genetic interaction reveals a new level for dS6K regulation. Gal4 drivers (Da-Gal4 and Act-Gal4). Note that the Da-Gal4 driver is more efficient than the Act-Gal4 driver to suppress ago expression levels as compared to controls (driver alone).

3.9.2-A putative Ago interaction motif in dS6K

Archipelago (ago) encodes a 1,326 amino-acid protein, that contains an F-box and seven WD repeats in its carboxyl terminal portion (section 1.4.4 in Introduction and and the proteins encoded by CG13822, CG4998, CG2165 [START_REF] Formstecher | Protein interaction mapping: a Drosophila case study[END_REF][START_REF] Alfarano | The Biomolecular Interaction Network Database and related tools 2005 update[END_REF][START_REF] Giot | A protein interaction map of Drosophila melanogaster[END_REF]. The WD repeat domain of Ago has been reported to be necessary for binding to Cyclin E and dMyc. Comparison of their polypeptidic sequence suggests that the S/T-P-x-x-S/T motif might be the consensus recognized by Ago in the substrate (S: Serine; T: Threonine; P: Proline) [START_REF] Moberg | The Drosophila F box protein archipelago regulates dMyc protein levels in vivo[END_REF]. Studies in Saccharomyces cerevisiae suggests that once phosphorylated, this motif constitutes an high-affinity phospho-epitope for binding to the WD repeat domain [START_REF] Nash | Multisite phosphorylation of a CDK inhibitor sets a threshold for the onset of DNA replication[END_REF] and the subsequent ubiquitination of the substrate for proteosome-dependent degradation [START_REF] Hao | Structure of a Fbw7-Skp1-cyclin E complex: multisite-phosphorylated substrate recognition by SCF ubiquitin ligases[END_REF][START_REF] Orlicky | Structural basis for phosphodependent substrate selection and orientation by the SCFCdc4 ubiquitin ligase[END_REF][START_REF] Moberg | Archipelago regulates Cyclin E levels in Drosophila and is mutated in human cancer cell lines[END_REF][START_REF] Mortimer | The Drosophila F-box protein Archipelago controls levels of the Trachealess transcription factor in the embryonic tracheal system[END_REF][START_REF] Mortimer | Regulation of Drosophila embryonic tracheogenesis by dVHL and hypoxia[END_REF][START_REF] Moberg | The Drosophila F box protein archipelago regulates dMyc protein levels in vivo[END_REF]. Sequence analysis revealed that this Ago binding consensus can be found in each of the interactor proteins mentioned above (Fig. 34). Primary structure of dS6K contains such an interacting motif in the auto-inhibitory domain of dS6K, which includes S 418 , P 419 and T 422 (Fig. 34 & Fig. 35). This auto-inhibitory domain is conserved in the mammalian S6K and has been shown to be critical for its activation in mammalian cells [START_REF] Dennis | Phosphorylation sites in the autoinhibitory domain participate in p70(s6k) activation loop phosphorylation[END_REF]. In Drosophila, the activity of dS6K is regulated by the phosphorylation of multiple serine and threonine residues [START_REF] Stewart | The Drosophila p70s6k homolog exhibits conserved regulatory elements and rapamycin sensitivity[END_REF], that sequentially involves the auto-inhibitory domain (S 418 , T 422 ), the linker domain (T 398 ) and the catalytic domain (T 238 ) (Fig 35). We therefore, tested three different variants of dS6K in which acidic amino acids were substituted for the conserved S/T phosphorylation sites in the auto-inhibitory and in the linker domains (Fig. 35). Two amino acids in the dS6K auto-inhibitory domain S 418 and T 422 were substituted to aspartic acid (D) and glutamic acid (E) respectively (UAS-dS6K STDE ); the T 398 residue in the linker domain was replaced with a glutamic acid (UAS-dS6K TE ); and all the those substitutions were present in the third variant (UAS-dS6K STDETE ) (Fig. 35). When co-expressed with ago RNAi , both UAS-dS6K TE and UAS-dS6K STDE produced a strong genetic interaction (Fig. 35B, D, H, J) as compared to the dS6K variants induced alone (Fig. 35A, C, G, I). Strikingly, co-expression of ago RNAi with the UAS-dS6K STDETE variant did not enhance the wing phenotype, but rather suppressed the bending-down of the wing due to UAS-dS6K STDETE induced alone (Fig. 35F,L). In summary, although these finding suggest that Ago regulates dS6K in a phosphorylation dependent manner, the molecular mechanism of this regulation remains to be elucidated. domains of the S6K are also indicated (From [START_REF] Barcelo | Altering Drosophila S6 kinase activity is consistent with a role for S6 kinase in growth[END_REF].

3.9.3-Genetic interaction between dS6K and ago mutants

To further investigate the genetic interactions between dS6K and ago, we generated double mutants; either of them exhibit a severe phenotype, since the former is semilethal [START_REF] Montagne | Drosophila S6 kinase: a regulator of cell size[END_REF] and the latter is lethal at early larval stage (data not shown). Since both genes are closely located on the left arm of chromosome 3, the mutants were recombined with an FRT cassette that allows for clonal analysis. We analyzed the FRT-associated mutations in the eye of heterozygous mutant flies using the eyeless promoter to drive flipase during eye development. As the FRT chromosome arm being wild-type for dS6K and ago also contained a homozygous cell-lethal Minute mutation [START_REF] Montagne | The nuclear receptor DHR3 modulates dS6 kinase-dependent growth in Drosophila[END_REF]; the recombined sister cells, homozygote wild type for dS6K and/or ago, were eliminated during development. This led to adult eyes that were largely made up of homozygous mutant cells. This way, we analyzed dS6K null mutant [START_REF] Montagne | Drosophila S6 kinase: a regulator of cell size[END_REF], ago 1 and ago 3 mutants [START_REF] Moberg | Archipelago regulates Cyclin E levels in Drosophila and is mutated in human cancer cell lines[END_REF] as well as the recombined double mutants. As expected, homozygous dS6K mutant eyes were smaller than controls (compare Fig 36B to 36A). In contrast homozygous mutant eyes for either the ago 1 or ago 3 mutations, did not show a significant modification in eye size (Fig. 36E,I). The absence of visible effect is a consequence of opposite effects of the ago mutation, the loss-of-function of which has been earlier shown by others to provoke an increase of cellular growth and apoptosis simultaneously [START_REF] Nicholson | The archipelago tumor suppressor gene limits rb/e2f-regulated apoptosis in developing Drosophila tissues[END_REF]. Importantly, the size of double dS6K-ago mutant eye was nearly identical to the size of dS6K mutant eye (Fig. 36B,F,J). This epitasis of dS6K mutation over ago mutants was further confirmed by the statistical measurement of the eye surface normalized to the corresponding size of the thorax (Fig. 37). Although, most of the eyes are made up of homozygous mutant cells, few heterozygous ommatidia are not eliminated allowing such measurements [START_REF] Nicholson | The archipelago tumor suppressor gene limits rb/e2f-regulated apoptosis in developing Drosophila tissues[END_REF]. To specifically analyze the role of dS6K and ago mutations on cellular growth only, the experiment was performed in an H99 background, which suppresses apoptosis [START_REF] White | Genetic control of programmed cell death in Drosophila[END_REF].

The H99 deficiency, which removes the hid, reaper and grim pro-apoptotic genes, is also located on the left arm of the chromosome 3. Recombination of the H99 deficiency with ago mutations has been previously shown to be appropriate for the visualization of the overgrowth that results from loss-of-ago function [START_REF] Nicholson | The archipelago tumor suppressor gene limits rb/e2f-regulated apoptosis in developing Drosophila tissues[END_REF]. Consistently, homozygous ago 1 or ago 3 mutant eyes were bigger when combined with the H99 deficiency (Fig. 36G,K). Nonetheless, the triple FRT-ago-dS6K-H99 homozygous mutant eyes were smaller and their size was nearly similar to the size of double FRT-dS6K-H99 mutant eyes (compare Fig. 36H, 36L to 36D).

Statistical measurement of the eye sizes normalized to the thorax size confirmed that, similar to the epitasis observed in the H99 wild-type background, the dS6K mutation was epistatic to the ago mutations in the H99 deletion background (Fig. 37). Altogether these findings indicate that the overgrowth resulting of loss-of ago function needs a functional dS6K activity to proceed.

-Ago regulates dS6K levels

Our findings strongly support the notion that Ago negatively regulates dS6K. Since previous studies clearly established that Ago regulates ubiquitin ligation and the subsequent proteasome degradation [START_REF] Hao | Structure of a Fbw7-Skp1-cyclin E complex: multisite-phosphorylated substrate recognition by SCF ubiquitin ligases[END_REF][START_REF] Orlicky | Structural basis for phosphodependent substrate selection and orientation by the SCFCdc4 ubiquitin ligase[END_REF][START_REF] Moberg | Archipelago regulates Cyclin E levels in Drosophila and is mutated in human cancer cell lines[END_REF][START_REF] Mortimer | The Drosophila F-box protein Archipelago controls levels of the Trachealess transcription factor in the embryonic tracheal system[END_REF][START_REF] Mortimer | Regulation of Drosophila embryonic tracheogenesis by dVHL and hypoxia[END_REF][START_REF] Moberg | The Drosophila F box protein archipelago regulates dMyc protein levels in vivo[END_REF], we hypothesized that Ago might regulate the degradation of dS6K. To investigate this issue, dS6K levels were first analyzed with a dS6K specific antibody [START_REF] Montagne | Drosophila S6 kinase: a regulator of cell size[END_REF] in ap-Gal4>ago RNAi wing imaginal discs where ago is repressed in the dorsal compartment. Nonetheless no difference could be detected in the levels of either the genuine (compare Fig 38A and 38C) or the over-expressed dS6K (compare Fig 38B and 38D).

Since, the genuine dS6K protein is barely detectable in Drosophila tissues, we took advantage of a transgene that constitutively expressed high levels of dS6K under the control of an α-tubulin promoter [START_REF] Montagne | Drosophila S6 kinase: a regulator of cell size[END_REF]. In contrast with the low expression of the genuine dS6K (Fig 38), the over-expressed kinase could be detected in most of the tissues. The dS6K expression levels was particularly high in the ring gland, where the detection was specific, as the signal dramatically dropped in flip-out clones expressing dS6K RNAi (Fig. 39A-C). However, the levels of neither the genuine (data not shown), nor the over-expressed dS6K was modified in flip-out clones expressing ago RNAi in imaginal discs (Fig. 39D-F). In contrast, a moderate increase of dS6K levels could be observed in ago RNAi flip-out clones generated in the salivary gland (Fig. 39G-I). These findings suggest that in a given tissue, Ago can regulate dS6K level. To get further insights into the role of Ago, the constitutive dS6K transgene was combined to the ago mutants. Consistent with the ago RNAi flip-out clones, dS6K levels in imaginal discs of third instar larvae were unaffected in either FRT-ago 1 and FRTago 3 mutant clones (Fig. 40A-C and data not shown). Surprisingly, FRT clones generated in endoreplicative tissues provoked a decrease of dS6K levels. In salivary glands (Fig. 40D-F), dS6K protein was barely detectable in FRT-ago 1 mutant clones;

whereas it was reduced in fat body FRT-ago 1 clonal cells (Fig. 40G-I). This unexpected results may be an indirect consequence of the ago mutation, which may have strong deleterious effects as the nucleus of homozygous ago 1 mutant cell looks disorganized (Fig. 40I). Since nucleus disruption may be due to the pro-apoptotic effect of the ago 1 mutation, dS6K levels were then analyzed in double FRT-ago 1 H99 mutant clones. In this genetic context, endoreplicative homozygous mutant cells were smaller but the nucleus was not disorganized, although its size was severely reduced as compared to the nuclei of control neighboring cells (Fig. 40J-L). This growth defect observed only in endcoreplicative cells must be a consequence of impaired Cyclin E degradation, which typically occurs in ago mutant cells [START_REF] Moberg | Archipelago regulates Cyclin E levels in Drosophila and is mutated in human cancer cell lines[END_REF][START_REF] Shcherbata | The mitotic-to-endocycle switch in Drosophila follicle cells is executed by Notchdependent regulation of G1/S, G2/M and M/G1 cell-cycle transitions[END_REF], since it was earlier described that the endoreplication process requires a cyclic expression of Cyclin E [START_REF] Follette | Fluctuations in cyclin E levels are required for multiple rounds of endocycle S phase in Drosophila[END_REF][START_REF] Weiss | Continuous Cyclin E expression inhibits progression through endoreduplication cycles in Drosophila[END_REF][START_REF] Edgar | Endoreplication cell cycles: more for less[END_REF]. Nonetheless no modification of dS6K levels could be detected in the FRT-ago 1 H99 mutant clones (Fig. 40K). Altogether, these findings suggest that in a strong ago mutant background many cellular functions may be affected; a genetic context that eventually does not allow the detection of potential cell-autonomous modification of dS6K protein levels.

Since, Western-blotting to the genuine dS6K was very efficient in S2 cells treated with dsRNA (Fig. 28), similar experiments were performed in third instar larvae expressing either of the ago RNAi lines directed by the ubiquitous da-Gal4 or act-Gal4 drivers. This genetic context does not affect viability (data not shown), most probably because expression of the ago mRNA is suppressed but not abolished (Fig. 33). As shown in Fig. 41, driving ago RNAi with either of the ubiquitous drivers led to an increase in dS6K levels. This increase in dS6K levels was however not observed in each experiment (data not shown), most probably because of the variability of the Western-blotting with protein extracts from 2 nd instar larvae, using antibody specific to dS6K (top), phospho-T398 residue of dS6K (middle) and α-Tubulin (bottom). As compared to wild type (control), in trans-heterozygous ago 1 /ago 3 mutant combination (ago -/-) dS6K levels are severely induced (quantification in C), whereas T398 phosphorylation is weakly decreased (quantification in D). Both dS6K and P-dS6K

were quantified and normalized to α-Tubulin using ImageJ (C and D).

To identify novel regulators of Notch signalling and asymmetric cell division, Mummery-Widmer and colleagues screened a total of 20,262 transgenic RNAi lines corresponding to 11,619 protein-coding genes [START_REF] Mummery-Widmer | Genome-wide analysis of Notch signalling in Drosophila by transgenic RNAi[END_REF]. The RNAi lines were induced using the pannier (pnr)-Gal4 driver that is active in the central part of the notum (dorsal thorax). showing enrichment in multiple pathways including TOR and insulin signalling, and the CCR4-NOT complex implicated in transcriptional regulatory mechanisms. Specific knock-down in drosophila and mouse revealed that the role of the CCR4-NOT complex to control cardiac contractility is conserved throughout evolution [START_REF] Neely | A global in vivo Drosophila RNAi screen identifies NOT3 as a conserved regulator of heart function[END_REF].

In our screen for steroidogenesis using the phm-Gal4 driver, about 11,000 lines, representing roughly 7,000 genes were scored, allowing the identification of 620 candidates (8.86 %). The same collection of RNAi lines was also screened for the regulators of dS6K-dependent growth using ap-Gal4 driver, leading to the selection of 357 enhancer RNAi lines (3.2 %). A more narrow and stringent selection ends up with a list of 45 genes whose RNAi induced a strong enhancement (0.64 %). In the screen for lipid metabolism at the fat body, approximately 7,300 RNAi lines representing 4000 genes were scored in flip out clones, leading to the identification of 24 candidate genes (0,6 %), the corresponding RNAi of which provoked an increase of LD size.

Comparison of our findings and data published by others revealed that the percentage of candidate genes selected varies a lot regarding the screening strategy. This number can be very high when the Gal4 driver target an essential tissue and the phenotype monitored is not precisely defined. For instance, 20.2 % of the tested genes induced lethality when the RNAi are targeted to muscles [START_REF] Schnorrer | Systematic genetic analysis of muscle morphogenesis and function in Drosophila[END_REF]. The high number of candidates found in our screen for steroidogenesis, therefore, suggests that a significant number of the selected genes affect general cellular processes, rather than the steroidogenesis pathway per se. Congruently, the classification of the genes regarding their presumptive functions (Fig 19) revealed that some of these candidate genes retain housekeeping activities, for instance, general transcription factors for RNA polII-dependent transcription or ribosome biogenesis. Thus, this screen needs further rounds of selection to restrict the candidate list to the genes that play specific roles, critical for steroidogenesis. These secondary assays will first rely on an in silico elimination of housekeeping genes and then on the direct observation of the prothoracic gland to discard the genes, the RNAi of which are deleterious for gland cell survival.

Strikingly, only 24 candidates (0.6 %) were identified through our screen for lipid metabolism at the fat body following the size of LDs, whereas Pospislik and colleagues identified 516 RNAi lines (4.5 %) as potential candidates when screening for triglyceride content (Pospislik et al., 2010). Although they screened about three times more RNAi lines than us, and hence, very low no. of of genes that we found suggest that the modification of LDs size is controlled by a limited set of genes and thus constitutes a very specific parameter for screening.

The screen for dS6K regulators first led to the selection of 357 enhancer RNAi lines (3.2 %). Considering that we were searching for negative regulators of dS6Kdependent growth, this percentage seems rather high. Nonetheless, it could be speculated that RNAi expression of several negative regulators of growth might be selected through our screening strategy. However, an RNAi to the negative regulator may not be able to induce an overgrowth of the dorsal wing blade when induced alone with the ap-Gal4 driver, while co-induction of the UAS-dS6K might create a sensitized background appropriate to the visualized subtle extra-growth effects. In agreement with potential dS6K unrelated effects, a previous screen for positive regulator of dS6K-dependent growth following the same phenotypic enhancement identified a rather high number of candidates. This candidate list was easily decreased when considering only the strong enhancements, which finally revealed a small subset of gene products that most-likely intervene in the dS6K signalling pathway [START_REF] Montagne | The nuclear receptor DHR3 modulates dS6 kinase-dependent growth in Drosophila[END_REF]. Following the same strategy, our candidate list was restricted to only 45 strong enhancers (0.64 %) which were further used to generate an interaction map.

-RNAi screening for new regulators of lipid metabolism

Many of the analogous organ systems that control nutrient uptake, storage, and metabolism in humans are present in the fruit fly [START_REF] Baker | Diabetic larvae and obese flies-emerging studies of metabolism in Drosophila[END_REF]. In Drosophila, the fat body accumulates nutrients mostly as LDs that contain triglycerides and proteins [START_REF] Britton | Drosophila's insulin/PI3-kinase pathway coordinates cellular metabolism with nutritional conditions[END_REF][START_REF] Beller | COPI complex is a regulator of lipid homeostasis[END_REF]. Starvation has been known to induce an increase of size of lipid droplets. This phenotype is also observed upon modulation of various growth or metabolic regulators, in particular with the intermediates of nutrient responsive TOR signaling pathway [START_REF] Hietakangas | Regulation of tissue growth through nutrient sensing[END_REF][START_REF] Britton | Drosophila's insulin/PI3-kinase pathway coordinates cellular metabolism with nutritional conditions[END_REF][START_REF] Beller | COPI complex is a regulator of lipid homeostasis[END_REF][START_REF] Colombani | A nutrient sensor mechanism controls Drosophila growth[END_REF][START_REF] Zhang | Regulation of cellular growth by the Drosophila target of rapamycin dTOR[END_REF][START_REF] Diangelo | Regulation of fat cell mass by insulin in Drosophila melanogaster[END_REF].

To identify novel regulators of growth and metabolism centred on the TOR/S6K cassette, we have retained the size of LDs as a specific hallmark to screen the RNAi collection of NIG that targets about half of the Drosophila genome. We, however, screened only 7,300 RNAi lines representing roughly one fourth of the genome. This way, 24 candidate genes were identified, the RNAi of which induced in fat body cells provokes an increase of the size of the LDs. Strikingly, none of these RNAi lines produced a phenotype as strong as the one induced in our pilot experiment with an RNAi to Rheb, which encodes an upstream effector in the TOR signalling pathway. These findings suggest that one of the Fasciclin genes might control the regulation of lipid metabolism. This also gives an idea that the mild effect in the size of lipid droplets due to Fasciclin2 could be due to any of the potential off-targets. This emphasizes a further detailed study for an invaluable insight of the overlapping of lipid metabolism and nervous system development processes. Although, only one fourth of the genome was screened, our finding indicates that Rheb and the downstream TOR play a major role in the regulation of LD size and further confirm that this pathway is central in responding to nutrients availability. Furthermore, the signalling molecules identified through our screen may be alternative modulator of this pathway.

During the course of our screen for LDs size modification, two distinct studies have been published that report screenings for lipid metabolism in Drosophila. As mentioned above, Pospislik and colleagues screened for triglyceride storage using 11,594 RNAi lines (Pospislik et al., 2010). Gene ontology highlighted the involvement of regulators of feeding control, nutrient transport, chromatin remodelling, cell cycle and development, but also the components of protein translation and degradation machineries. In another study, Guo and colleagues screened the entire Drosophila genome with RNAi treatment in cultured S2 cells, following the aspect of LDs as a specific hallmark for lipid metabolism. The 180 genes identified this way, encode regulators and enzymes of lipid metabolism, members of translation machinery, subunits of RNApolymerase II, splicosome or proteasome, and effector of vesicle trafficking. They also demonstrated 5-different phenotypic classes regarding the size, the number and the aspect of LDs. These phenotypic features, could not be directly transposed to fat body cells, since it was necessary to add high amounts of oleic acid in the media to trigger LD formation in the cultured S2 cells [START_REF] Guo | Functional genomic screen reveals genes involved in lipid-droplet formation and utilization[END_REF]. These epithelial-like diploid cells are originated from embryo and do not appear to be specialized in lipid storage and homeostasis. Each of those screens including ours, are different and do not provide overlapping results. In particular, none of the candidate genes found in our LD size screen was found in the S2 cell screen and only a potential calcium transporter (CG2165) was found in the Pospislik screen, the gene disruption of which leads to a decrease in triglyceride content (Pospislik et al., 2010). Consistent with the search for regulators and effectors of lipid metabolism, each of the screens identified enzymes that catalyzed synthesis, modification or degradation of lipids.

Strikingly, members of the translational machinery were also found in each of those screens, suggesting that translation efficiency contributes to the lipid metabolism. It is worth noting that LDs are typically constituted in lipids, mostly triglycerides, surrounded by a phospholipid monolayer and associated proteins [START_REF] Farese | Lipid droplets finally get a little R-E-S-P-E-C-T[END_REF][START_REF] Goodman | The gregarious lipid droplet[END_REF][START_REF] Beller | Characterization of the Drosophila lipid droplet subproteome[END_REF][START_REF] Cermelli | The lipid-droplet proteome reveals that droplets are a protein-storage depot[END_REF]. Therefore, it can be speculated that the increase of LD size is a direct consequence of protein synthesis failure, since packaging of large LDs requires fewer proteins than small LDs for an identical amount of lipids. This interpretation is consistent with the identification of three ribosomal protein gene in our screen and with the increase of LD size observed in mutant cells for the slif amino acid transporter [START_REF] Colombani | A nutrient sensor mechanism controls Drosophila growth[END_REF]. This further suggests that the increased of LD size induced by starvation, basically reflects an economic strategy for lipid packaging.

Unexpectedly, our screen for LD size identified several genes, for which the function was previously linked to the development or the activity of the CNS, indicating that these candidate genes also control basic metabolism. This is of particular interest, since lipid metabolism plays a critical role in the differentiation and activity of neurons.

Considering that modification of lipid metabolism is proposed to play a critical role in several neurological diseases. Thus, our screen may lead to the identification of novel regulators that potentially affect lipid metabolism in neurons, an issue that we wish to follow in a future collaborative project.

In summary, these three distinct studies highlight the role of enzymes of lipid metabolism and protein translation machinery in triglyceride storage and physiology of LDs, although each of the screens identified a distinct set of candidate genes. In contrast to the others, only 24 potential candidates were retained through our screening strategy, suggesting that the increase of LD size is a very specific parameter that does not reflect all the physiological aspects of lipid metabolism. However, I did not continue this LD project when the S2 screen was published [START_REF] Guo | Functional genomic screen reveals genes involved in lipid-droplet formation and utilization[END_REF], and concentrated on the screen for negative regulators of dS6K-dependent growth.

-RNAi screening for negative regulators of dS6K-dependentgrowth

Loss-of-function for dS6K provokes severe growth defect [START_REF] Montagne | Drosophila S6 kinase: a regulator of cell size[END_REF]. In contrast gain-of-function induced very subtle over-growth which could be visualized only when dS6K is induced in the developing dorsal wing compartment, leading to a bending-down of the adult wing. By monitoring an enhancement of the bending-down, this sensitized phenotype has been successfully used in a gain-of-function screen to identify novel positive regulators of dS6K-dependent growth [START_REF] Montagne | The nuclear receptor DHR3 modulates dS6 kinase-dependent growth in Drosophila[END_REF].

This screen failed to identify negative regulators, since suppression of the bendingdown of the adult wing happen to be a very frequent event. Hence, to identify novel negative regulators of dS6K-dependent growth we took advantage of the RNAi collection to screen for enhancement of the bending-down phenotype. This way, 11,000 lines roughly corresponding to 7,000 genes have been screened, leading to the selection of 45 genes, which fall in various functional categories, including ubiquitin ligase activity, metabolic enzymes and signalling molecules. Potentially, metabolic enzymes may act downstream of dS6K signalling to down-regulate processes, which could be activated by dS6K. Consistently these candidates catalyse enzymatic reactions of protein and lipid metabolism, two processes that may be regulated by the TOR signalling pathway (see above). Furthermore, dS6K has been shown to directly affect lipid storage regarding fly age [START_REF] Cho | Age-and diet-specific effects of variation at S6 Kinase on life history, metabolic, and immune response traits in Drosophila melanogaster[END_REF]. Signalling molecules rather act upstream of dS6K to integrate growth factor and nutrient signalling. Finally the candidate genes encoding protein with ubiquitin ligase activity might be of great help to characterize the mechanism of dS6K degradation since nothing is known yet about the regulation of this specific cellular process.

Systematic studies with Drosophila gene products revealed that seven proteins could physical interact with dS6K in Y2H assay [START_REF] Formstecher | Protein interaction mapping: a Drosophila case study[END_REF]. Interestingly, three of them (CG15010/ago, CG8843/sec5, CG12019/cdc37) were identified as potential negative regulators of dS6K; since their RNAi produced an enhancement of the bent-down wing phenotype. Although our screen uncovers only half of the Drosophila genome, the seven dS6K interactors were challenged in our sensitized setting. The RNAi to four of them (CG10622/sucb, CG10295/Pak, CG14216, CG8128) did not enhance the bent-down phenotype suggesting that they are not negative regulators of dS6K. In fact, an RNAi to a positive regulator of dS6K should suppress the bending-down of the adult wing, an event that is too much frequent (about 50%) to be retained as a relevant phenotype (data not shown).

The Drosophila genome-scale Databases for Y2H protein interactions [START_REF] Bork | Protein interaction networks from yeast to human[END_REF][START_REF] Formstecher | Protein interaction mapping: a Drosophila case study[END_REF] has been used in an attempt to set up a connecting network between the candidate genes identified in the screen. All the enhancer candidates were analyzed using web-based sources; "The biogrid" and "BOND", and thus, scored for the interaction with other partners. The network established this way (Fig. 27) revealed that all the candidate genes, for which Y2H interactive data were available, could be linked together; although additional proteins were required to fulfil some gap in the network. Nonetheless, more than half of these additional genes products, have been tested through our screen and only two of them were found to mildly enhance the bent-down wing phenotype. As mentioned above, these additional gene products are likely acting independently or as activators of dS6K, but certainly not as repressors, since only the latter could be identified in our screening strategy.

Protein-protein interaction networks are classically established with the list of candidate genes identified through genome-wide screening to highlight specific regulatory modules. Consistent with a role in regulating metabolism, the TOR signalling has been found as a critical determinant in the screen for triglyceride accumulation (Pospislik et al., 2010). This study also revealed that the Hedgehog signalling controls triglyceride accumulation, establishing a physiological link between morphogen signalling and metabolism. This link is further emphasized in our network, since several components of the Hedgehog signalling pathway (patched, fused, cubitus interuptus) appeared to be intermediates necessary for associating together our negative regulators of dS6K-dependent growth (Fig. 27). Alternatively identification of members of the Hedgehog signalling may only reflect the central role of this pathway in the morphogenesis of the wing [START_REF] Ogden | Regulation of Hedgehog signaling: a complex story[END_REF]. The discovery that ago is a negative regulator of dS6K-dependent growth also emphasizes the role of proteasomemediated degradation in this protein-protein interaction network. This finding might be of upmost interest, since Ago encodes an F-box Ubiquitin-ligase component, one of the very few proteins that physically interact with dS6K, thus providing a potential molecular insight into the regulation of dS6K degradation.

-Regulation of dS6K activity

In an attempt to identify some novel regulators of dS6K, the candidate genes, RNAi of which produced an extreme enhancement, were analyzed in S2 cells by Westernblotting after dsRNAi treatment. This way, three genes (Cdep, Nf1, CG10139) were found to regulate dS6K phosphorylation and one (ago) to regulate dS6K levels. The CG10139 encodes a small protein of 224 amino acids without a typical conserved domain. Sequence blast could not identify a mammalian homologue. The gene has not been studied yet and there is no available mutant [START_REF] Giot | A protein interaction map of Drosophila melanogaster[END_REF]flybase.org). That this gene is found only in insects suggesting that it might be a target for agronomic treatment. Nonetheless, extensive and deep analyses must be acquired to understand how CG10139 regulates the process of growth through dS6K phosphorylation.

The Drosophila CG8318 gene encodes a protein named as Neurofibromin 1 (NF1), the amino acid sequence of which is 60% identical to the unique human NF1 protein (Fig. 29). This is a large protein, which contains a GAP (GTPase activating protein) domain for Ras. In humans, mutation of the Nf1 tumor suppressor gene causes a familial heritable syndrome. Loss-of-heterozygosity favours the development of nervous system tumors including neurofibromitosis [START_REF] Ferner | Guidelines for the diagnosis and management of individuals with neurofibromatosis 1[END_REF]. Therefore, elevated

Ras activity was thought to contribute to the development of these tumors. However, Ras inhibitors-based therapy has a limited success. Following a proteomic approach, Dasgupta and colleagues [START_REF] Dasgupta | Proteomic analysis reveals hyperactivation of the mammalian target of rapamycin pathway in neurofibromatosis 1-associated human and mouse brain tumors[END_REF] observed an increase of ribosomal proteins expression in extracts of Nf1 mutant astrocytes associated with hyperphosphorylation of RPS6. They also observed that treatment with the TOR inhibitor rapamycin, impedes RPS6 phosphorylation and hyperproliferation of NF1 mutant astrocytes. Further studies have now clearly established that NF1 act as a repressor of the mTOR signalling both in cultured cells and in mouse tumors [START_REF] Johannessen | The NF1 tumor suppressor critically regulates TSC2 and mTOR[END_REF][START_REF] Johannessen | TORC1 Is Essential for NF1-Associated Malignancies[END_REF]. Since both Ras and mTOR are aberrantly activated in Nf1-deficient tumors suggesting that the hyper-activated Ras in these tissues leads to the activation of the PI3K pathway which in turn relieves the TSC2-medited down-regulation of mTORC1.

The finding that the Drosophila NF1 acts as a negative regulator of dS6K-dependent growth validates the relevance of our screen. Several other studies in Drosophila have shown that Nf1 plays an important role in learning and memory [START_REF] Margulies | Deconstructing memory in Drosophila[END_REF]. In addition, one study has already shown that loss of Drosophila NF1 resulted in a reduction in size of larvae, pupae and adult [START_REF] The | Rescue of Drosophila NF1 mutant phenotype by protein kinase A[END_REF]. This size defect could be restored by manipulating PKA signalling but not Ras. This growth defective phenotype is intriguing, since NF1 is reported to be a tumor suppressor that inhibits cellular growth. Moreover, we found that RNAi disruption of the Drosophila NF1 enhances an overgrowth induced by dS6K-over-expresion and increases dS6K phosphorylation. A possible explanation might be that NF1 is required to sustain systemic growth but acts as a repressor of cell-autonomous growth. In agreement with such a model, several studies have shown that mutation of PI3K leads to a cellautonomous growth defect [START_REF] Weinkove | Regulation of imaginal disc cell size, cell number and organ size by Drosophila class I(A) phosphoinositide 3-kinase and its adaptor[END_REF], whereas down-regulation of this pathway in the prothoracic gland increases systemic growth [START_REF] Colombani | Antagonistic Actions of Ecdysone and Insulins Determine Final Size in Drosophila[END_REF].

Analysis of homozygous mutant clones in an otherwise heterozygote mutant background must be an appropriate approach to address this issue. An alternative hypothesis is that the Nf1 null mutation could trigger a compensatory response that restrains cellular growth, whereas the down-regulation mediated by the RNAi is not sufficient to fully abolished NF1 expression and subsequently does not trigger this compensatory response. Hence, it could be speculated that this compensatory effect is lost in tumor cells.

The Drosophila CG1283 gene (Cdep) is conserved throughout evolution. It encodes a protein with several functional domains, including a FERM (Farp ezrin moesin radixin), a Rho GEF (Guanine nucleotide exchange factor) and 2 PH (pleckstrin homology) domains and hence, it is expected to be involved in adhesion, proliferation and/or differentiation [START_REF] Koyano | Molecular cloning and characterization of CDEP, a novel human protein containing the ezrin-like domain of the band 4.1 superfamily and the Dbl homology domain of Rho guanine nucleotide exchange factors[END_REF]. An elevated expression level of Cdep was observed in hypertrophic stage of chondrocytes [START_REF] Koyano | Chondrocyte-derived ezrin-like domain containing protein (CDEP), a rho guanine nucleotide exchange factor, is inducible in chondrocytes by parathyroid hormone and cyclic AMP and has transforming activity in NIH3T3 cells[END_REF]. A recent study has revealed that it is required for TGFβ mediated switch from cohesive to single cell motility in breast cancer progression. Nevertheless, these data do not reveal how Cdep contributes to cellular growth, since TGFβ promotes tumor cell motility, but also can promote growth arrest [START_REF] Giampieri | Localized and reversible TGF beta signalling switches breast cancer cells from cohesive to single cell motility[END_REF] Concurrent to the completion of our screen for repressors of dS6K-dependent growth, a genome-wide RNAi screen has been performed in S2 cells for identification of novel regulators of the TORC1/S6K module [START_REF] Lindquist | Genome-scale RNAi on living-cell microarrays identifies novel regulators of Drosophila melanogaster TORC1-S6K pathway signaling[END_REF]. Using a phospho-specific antibody to the ribosomal protein s6, the S6K genuine target, an immunofluorescence assay was designed to follow the activity of the TORC1/S6K module.

This way, 13,618 genes, representing roughly 97 % of the Drosophila genome, were monitored, leading the selection of 240 low-phospho-S6 and 139 high-phospho-S6

genes. The gene products selected in the latter group corresponds to potential repressors, but unexpectedly, none of them match to our list of candidates. These differential findings may rely on the readout. The bending-down of the wing is a highly sensitized phenotype; roughly a 1% increase in the length of the dorsal versus ventral blades produces an adult wing whose proximal/distal axis represents a quarter of circle [START_REF] Montagne | Drosophila S6 kinase: a regulator of cell size[END_REF]. Hence, our screening strategy can visualize very subtle modification of cellular growth. Moreover, the ap-Gal4 driver used for the screen is also active in some embryonic neurones and thus, might provoke lethality when driving RNAi to an essential gene [START_REF] Montagne | The nuclear receptor DHR3 modulates dS6 kinase-dependent growth in Drosophila[END_REF]. In contrast, only strong modifications of the fluorescent signal due to phospho-specific antibody can be easily detectable in the screen with S2 cells. In addition, it has been observed that phosphorylation of S6 is severely impaired in S6K1/2 double mutant but not totally abolished, suggesting that an alternative pathway contributes to this phosphorylation [START_REF] Pende | S6K1(-/-)/S6K2(-/-) mice exhibit perinatal lethality and rapamycin-sensitive 5'-terminal oligopyrimidine mRNA translation and reveal a mitogen-activated protein kinase-dependent S6 kinase pathway[END_REF]. In summary, Lindquist and colleagues performed a screen for strong regulators of S6 phosphorylation mostly dependent on the TORC1/S6K module [START_REF] Lindquist | Genome-scale RNAi on living-cell microarrays identifies novel regulators of Drosophila melanogaster TORC1-S6K pathway signaling[END_REF], whereas our screen identified specific negative modulators that likely fine-tune the activity or levels of dS6K. Considering that both screens provide a list of novel signalling components for S6K, it will be of fundamental interest to determine whether they act in distinct or common molecular processes.

-Does Archipelago control dS6K degradation?

Ago, the F bow component of SCF-ubiquitin ligase and the Drosophila ortholog of the mammalian F-box protein Fbw7 (SCFF bw7 ) has been implicated in the control of dMyc, cyclin E, Notch and Trh [START_REF] Moberg | Archipelago regulates Cyclin E levels in Drosophila and is mutated in human cancer cell lines[END_REF][START_REF] Moberg | The Drosophila F box protein archipelago regulates dMyc protein levels in vivo[END_REF][START_REF] Tetzlaff | Defective cardiovascular development and elevated cyclin E and Notch proteins in mice lacking the Fbw7 F-box protein[END_REF][START_REF] Mortimer | The Drosophila F-box protein Archipelago controls levels of the Trachealess transcription factor in the embryonic tracheal system[END_REF]. It has previously been shown that ago mutant cells proliferate more than wild type cells and contain an increased levels of Cyc E tissues. Our findings suggest that at least in some tissues Ago does control dS6K degradation in a cell-autonomous manner and that the increased dS6K levels observed in ago mutant larvae is a consequence of systemic effect. This hypothesis is however unlikely, since Ago controls cell growth in a cell-autonomous and can directly bind to dS6K in yeast 2 hybrid assay. Alternatively, the pleiotropic function of Ago, which regulates CycE, dMyc, Notch and Trh [START_REF] Moberg | Archipelago regulates Cyclin E levels in Drosophila and is mutated in human cancer cell lines[END_REF][START_REF] Moberg | The Drosophila F box protein archipelago regulates dMyc protein levels in vivo[END_REF][START_REF] Mortimer | The Drosophila F-box protein Archipelago controls levels of the Trachealess transcription factor in the embryonic tracheal system[END_REF] may have indirect consequence on dS6K. It has been described that the regulators of cell cycle machinery, i.e., dMyc, Notch, dE2F work in a context-dependent-manner (Herranz and Milàn, 2008). Therefore, perturbation in Ago which is known to regulate these proteins could lead to some striking findings.

Congruently, we observed a decrease of dS6K levels in clonal ago mutant fat body cells, which was reverted when the ago mutation was combined to the H99 deficiency.

This genetic context suppressed apoptosis [START_REF] Nicholson | The archipelago tumor suppressor gene limits rb/e2f-regulated apoptosis in developing Drosophila tissues[END_REF], although cell growth was still restricted, probably because of elevated Cyc E levels [START_REF] Moberg | Archipelago regulates Cyclin E levels in Drosophila and is mutated in human cancer cell lines[END_REF][START_REF] Shcherbata | The mitotic-to-endocycle switch in Drosophila follicle cells is executed by Notchdependent regulation of G1/S, G2/M and M/G1 cell-cycle transitions[END_REF] the expression of which must be cyclic to sustain endoreplication and growth of the polytenic tissues. It is therefore, conceivable that in a context of cell competition the increase of dS6K levels could not be visualized because of cell interactions consequent to the pleiotropic effects of Ago.

Nonetheless to deeply investigate the physical interaction between Ago and dS6K, the immunoprecipitation assay will be performed in S2 cells over-expressing the different variants of each partner. This study will be completed by an in vivo analysis of the expression levels of each dS6K variant in the context of ago loss-of-function clones using either RNAi or mutants.

In summary our study supports the notion Ago controls dS6K degradation, although several issues have still to be addressed. This phenomenon should be investigated in mammalian cells, since both the TOR/S6K signalling pathway and the SCF-dependent proteasome degradation process are directly implicated in various human pathologies.

Further investigations based on the other candidates selected through our screens may also contribute to the understanding of these regulations.

Figure 1 -

 1 Figure 1 -mTOR signaling ....................................................................................

Figure 2 -

 2 Figure 2 -The domain structure of TOR protein ...................................................

Figure 3 -

 3 Figure 3 -TOR complex 1 (TORC1) and TOR complex 2 (TORC2) ..................

Figure 4 -

 4 Figure 4 -Regulation of cell growth and cell cycle progression by TOR ............

Figure 5 -

 5 Figure 5 -Interrelations between TOR signaling, autophagy and cell growth ....

Figure 6 -

 6 Figure 6 -Structure of a lipid droplet ...................................................................

Figure 7 -

 7 Figure 7 -Comparison of carboxy-terminal domain of ribosomal protein S6 ......

Figure 8 -

 8 Figure 8 -The domain structure of S6K1, S6K2 and the dS6K ..........................

Figure 9 -

 9 Figure 9 -Model of S6K1 activation ....................................................................

Figure 10 -

 10 Figure 10 -dS6K and the regulation of growth ....................................................

Figure 11 -

 11 Figure 11 -The SCF complex and the Ubiquitination pathway ...........................

Figure 12 -

 12 Figure 12 -The domain structure of Archipelago (Ago) in Drosophila, human and C. elegans .........................................................................................

Figure 13 -

 13 Figure 13 -Dicer and RISC (RNA-induced silencing complex) ..........................

Figure 14 -

 14 Figure 14 -RNAi interference in Drosophila ........................................................

Figure 15 -

 15 Figure 15 -The structure and location of the ring gland in drosophila larva .......

Figure 16 -

 16 Figure 16 -The dynamics of ecdysteroids levels and the development of drosophila ..........................................................................................

Figure 17 -

 17 Figure 17 -Schematic diagram of the genome-wide RNAi screen for novel regulators of dS6K-dependent growth ..............................................

Figure 18 -

 18 Figure 18 -Schematic diagram of the genome-wide RNAi screen for novel regulators of lipid metabolism and steroidogenesis ..........................

Figure 19 -

 19 Figure 19 -The phenotypic classification of the Phm screen ..............................

Figure 20 -

 20 Figure 20 -Lipid droplets labelled with LSD2-GFP fusion protein ......................

Figure 21 -

 21 Figure 21 -Changes in the size of lipid droplet (LD) by Rheb RNAi .......................

Figure 22 -

 22 Figure 22 -The phenotypic classification of the Lipid droplet screen .................

Figure 23 -

 23 Figure 23 -Changes in the size of lipid droplet (LD) by Fas RNAi .........................

Figure 24 -

 24 Figure 24 -Modulation of the bend-down wing phenotype due to dS6K overexpression .........................................................................................

Figure 25 -

 25 Figure 25 -A brief summary of RNAi lines identified in a screen for modulators of the dS6K-dependent wing phenotype ...............................................

Figure 26 -

 26 Figure 26 -A representation of dS6K interactors ..............................................

Figure 27 -

 27 Figure 27 -A protein-protein interaction network centered on dS6K-dependent growth ..............................................................................................

Figure 28 -

 28 Figure 28 -Western blot analysis to the extracts of S2 cells for dS6K and P-dS6K ................................................................................................

Figure 29 -

 29 Figure 29 -Sequence analysis of CG8318 (Neurofibomin 1, NF1) ..................

Figure 30 -

 30 Figure 30 -Sequence analysis of CG1283 (Cdep) ...........................................

Figure 31 -

 31 Figure 31 -Sequence analysis of CG15010 (Archipelago) ...............................

Figure 32 -

 32 Figure32-Genetic interaction at the wing between dS6K and ago RNAi ...........

Figure 33 -

 33 Figure 33 -Efficiency of ago RNAi through quantitative PCR ...............................

Figure 34 -

 34 Figure 34 -The Ago substrates share a putative interactive motif ....................

Figure 35 -

 35 Figure 35 -Genetic interaction between ago RNAi and different variants of dS6K .........................................................................................................

Figure 36 -

 36 Figure 36 -Genetic interaction between dS6K and ago in homozygous mutant eyes .................................................................................................

Figure 37 -

 37 Figure 37 -A representation of the eye/thorax ration between dS6K and ago mutants ............................................................................................

Figure 38 -

 38 Figure 38 -Immunostaining to dS6K in wing imaginal disc of the 3 rd instar larvae .........................................................................................................

Figure 39 -

 39 Figure 39 -dS6K expression level and flip-out clones ......................................

Figure 40 -

 40 Figure 40 -dS6K expression level in ago mutant clones ..................................

Figure 41 -

 41 Figure 41 -Western blot analysis to dS6K in ago RNAi and ago mutant larval extracts ............................................................................................

  Drosophila homologs of F-box components and related genes .......... Table 2 -List of RNAi lines identified in screen for LD size ................................. Table 3 -List of RNAi lines identified as strong enhancers of dS6K-dependent growth ................................................................................................

Figure 1

 1 Figure 1 -mTOR signaling TOR intercepts different signaling pathways and thereby controls processes such as transcription, translation, ribosome biogenesis, autophagy, mitochondrial metabolism, apoptosis, and the reorganization of the actin cytoskeleton (From Soulard and Hall, 2007).

Figure 2 -

 2 Figure 2 -The domain structure of TOR protein All TOR proteins contain in their carboxy-terminus about a 250 amino acid region that shows homology to the catalytic subunit of PI-3/4 kinases (violet). Despite the homology to lipid kinases, the TOR kinase domain phosphorylates serine and threonine residues of target proteins. The central about 550 residue long FAT domain occurs always in tandem with the 35 residue encompassing FATC motif at the very carboxy-terminal end. The FRB (FKBPrapamycin binding) domain is located between the FAT and the kinase domains. Two aminoterminal domaines composed of HEAT repeats are supposed to mediate protein-protein interactions (drawn from[START_REF] Wullschleger | TOR signaling in growth and metabolism[END_REF].

Figure 3 -

 3 Figure 3 -TOR complex 1 (TORC1) and TOR complex 2 (TORC2) TOR-associated proteins (KOG1, TCO89, LST8, AVO1-3, and BIT61) and their potential interactor domains in TOR (HEAT, FAT, FRB, Kinase, and FATC). Both TORC1 and TORC2 are likely dimers of multi-proteins containing complexes. TORC1

Figure 4 -

 4 Figure 4 -Regulation of cell growth and cell cycle progression by TOR TORC1 promotes cap dependent translation by activating S6K and inhibiting 4E-BP. This regulation promotes bulk protein biosynthesis and subsequent cell growth. (From Tee and Blenis, 2005).

Figure 6 -

 6 Figure 6 -Structure of a lipid droplet Lipid droplets are the lipid storage organelles of all organisms, and share a simple, stereotyped structure of a hydrophobic core built of the storage lipids (triacylglycerols), surrounded by a phospholipid monolayer to which numerous proteins are attached (From

Figure 9 -

 9 Figure 9 -Model of S6K1 activation S6K1 remains repressed in an inactive conformation in the absence of stimuli. The protein phosphatase PP2A has been implicated in binding to and inactivating S6K1 (A). Growth factors and nutrients are thought to induce m-TOR-mediated phosphorylation of S6K1 (B). In a first step, it is thought that the Ser/Thr-Pro sites in the autoinhibitory domain become phosphorylated. Phosphorylation of the sites in the autoinhibitory domain is thought to facilitate mTOR-mediated phosphorylation of key regulatory sites within the linker domain (Ser 371 and Thr 389 ), leading to a depressed kinase conformation.Finally, PDK1 docks onto S6K1 phophorylated at Thr 389 and activates the kinase by inducing phosphorylation at the activation loop site (Thr 229 ) in the catalytic domain (C)(From Radimerski, 2002).

Figure 10 -

 10 Figure 10 -dS6K and the regulation of growth dS6K is implicated in the regulation of growth. Homozygous dS6K null mutation (dS6K l/l ) flies (right) are reduced in overall body size compared to control (left). It is worth noting that the flies lacking dS6K are reduced in size due to smaller but not less cells (From Montagne et al., 1999).

Figure 11 -

 11 Figure 11 -The SCF complex and the Ubiquitination pathway The SCF complex consists of four major components: Skp1, Cul1, ROC1 and an F-box protein. Sul1 is a scaffold protein, its C-terminus interacts with Roc1, which contains a characteristic RING-finger domain to recruit Ub-E2; the N-terminus interacts with Skp1, which binds the conserved F-box domain of an F-box protein. The protein degradation pathway is mediated by ubiquitin (Ub); a small peptide of 76 amino acids, covalently attached to substrates targeted for degradation by three consecutive enzymatic reactions. The E1 activating enzyme forms a high-energy thioester bond with Ub by consuming ATP, and transfers the bound Ub to the E2 conjugating enzyme. Using Ub from E2, E3 ligases recognize protein substrates and catalyze Ub conjugation and polymerization. The HECT ligases form a thioester linkage with Ub from E2 and then transfer Ub to the substrate (Sb), whereas RING ligases interact with both Ub-E2 and the substrate for ubiquitination. The poly-Ub chains on the substrate are recognized by the 26S proteasome for subsequent proteolysis. Ubiquitination is reversible, by deubiquitinating enzymes, which are thiol proteases that cleave poly-Ub chains or Ub conjugated proteins. The Ub degradation pathway can be regulated in the processes of substrate recognition, Ub conjugation, deubiquitination and recruitment to the 26S proteasome

  Hariharan et al. performed a genetic screen in Drosophila to identify genes involved in controlling cell numbers and growth during development and found Archipelago (ago) as a candidate gene[START_REF] Moberg | Archipelago regulates Cyclin E levels in Drosophila and is mutated in human cancer cell lines[END_REF]. The screen was done by generating clones of homozygous mutant tissue in fly eyes and selecting the mutant cells that had a proliferative advantage over the wild type cells. Within the ago mutant clones, distance between adjacent photoreceptor clusters was increased compared to the wild-type, indicating an over-proliferation in the mutant tissue. This over-proliferation of ago mutant tissue is not due to irregular apoptosis event since there is no significant decrease in the extent of cell death. Due to a role in cell proliferation, loss of ago could lead to an increased level of a positive cell cycle regulator. Several cyclins and CDKs were examined, and the Cyclin E was found to accumulate in the ago mutant clones in third instar eye imaginal discs. Nevertheless, the mRNA level of cyclin E remains unchanged within ago mutant clones, suggesting a post-transcriptional modification of Cyclin E by

Figure 12 -

 12 Figure 12 -The domain structure of Archipelago (Ago) in Drosophila, human and C. elegans Archipelago (ago) encodes a protein with an F-box and WD repeats. The domain structure; the F-box (light blue), WD-repeat region (pink) and the ago alleles are shown. The N-terminal domains of the two human and the unique C. elegans and Drosophila Ago are indicated; note the absence of conserved domain. The degree of conservation in the F-box/WD region between Drosophila, human ago and C. elegans sel-10 is also indicated (identity/similarity).

Figure 13 -

 13 Figure 13 -Dicer and RISC (RNA-induced silencing complex) RNAi is initiated by the Dicer enzyme which processes double-stranded RNA into ~22nucleotide small interfering RNAs. The siRNAs are incorporated into a multi-component nuclease, RISC. RISC must be activated from a latent form, containing a double-stranded siRNA to an active form, RISC*, by unwinding of siRNAs. RISC* then uses the unwound siRNA as a guide to the substrate selection (drawn from Hannon, 2002).

Figure 14 -

 14 Figure 14 -RNAi interference in Drosophila Double-stranded RNA (dsRNA) that is homologous to the target gene by either of two methods. Injection of in vitro transcribed RNA into individual embryo or expression of an inverted repeat in vivo. The dsRNA is processed into 21-25 nucelotide (nt) small interfering RNAs by the Dicer ribonuclease. The siRNA is used to guide the sequence specific degradation of mRNA, leading to post-transcriptional silencing of the target locus (drawn from Adams and Sekelsky, 2002).

Figure 16 -

 16 Figure 16 -The dynamics of ecdysteroids levels and the development of drosophila The transient increase in ecdysteroids levels during the course of development in drosophila. The ecdysteroids are required for morphogenetic events and for cuticle deposition in embryos. During larval and pupal stages, ecdysteroid levels rise briefly controlling the transition from one stage to the next and regulate the onset of metamorphosis.

Figure 17 -

 17 Figure 17 -Schematic diagram of the genome-wide RNAi screen for novel regulators of dS6K-dependent growth (Top) Males carrying the inducible RNAi (UAS-RNAi) were crossed to virgin females of the genotype ap-Gal4,UAS-dS6K (F0 flies). The offspring (F1 generation) was scored for the enhancement of the adult bent-down wing phenotype. (Bottom) The corresponding lines were tested second time (right) and further analyzed to discard the RNAi lines that produced a bent-down of the wing when induced alone (left). The selected candidates were further used to generate an interaction network.

Figure 18 -

 18 Figure 18 -Schematic diagram of the genome-wide RNAi screen for novel regulators of lipid metabolism and steroidogenesisThe inducible RNAi males (UAS-RNAi) were crossed to virgin females of Sp;MKRS/SM5-TM6B double balancer flies (F0). The balanced RNAi males (UAS-RNAi/SM5-TM6B) were crossed separately to the corresponding virgin females expressing Gal4 (F1) either in the steroidogenesis organ (phm screen) (right) or in randomly induced clones (lipid metabolism screen) (left). The phenotypes of the Tubby + larvae, pupae and/or adults were analyzed carefully during screening for steroidogenesis (F2). For lipid metabolism screen, the tubes were heat-shocked after two days of egg-laying. At the 5 th day, the Tubby + L3 larvae were dissected for lipid droplet analysis in the fat body (F2). The fat body for the positive candidates was then stained with ORO (oil red O) or Nile red for further analysis of lipid droplet phenotype.

Figure 19 -

 19 Figure 19 -The phenotypic classification of the Phm screen (A) The 620 candidate genes retained in the screen for regulators of steroidogenesis were categorized into 15 sub-groups regarding their biological functions. The largest sub-group represents genes, which have not been annotated yet. (B) The observed phenotypes were categorized into 9 sub-groups. Formation of the giant third instar larvae (L3) was the most frequent phenotype found in the screen. The different other indicated phenotypes are described in the text.

Figure 20 -

 20 Figure 20 -Lipid droplets labelled with LSD2-GFP fusion protein Variation of size of the lipid droplets labelled with an Lsd2-GFP fusion protein upon gene disruption by UAS-RNAi induced within the entire fat body. The ppl-Gal4>UAS-Lsd2-GFP were crossed to an RNAi that induced a decrease of LD size (left) as compared to a mock RNAi without consequence (right).

Figure 21 -

 21 Figure 21 -Changes in the size of lipid droplet (LD) by Rheb RNAi A pilot experiment using Rheb shows efficacy of flip-out technique. Note that the size of LDs is significantly increased (GFP positive cells), and the number of LDs per cell in clones is decreased compared to the surrounding control cells (GFP negative cells). Gal4 expressing clones were randomly induced by heat-shock to activate a UAS-GFP and a UAS-Rheb-RNAi. The GFP positive clones are detected in fluorescence (A) whereas LDs are visualized in Nomarski (B). Both pictures are merged (C).

Figure 22 -

 22 Figure 22 -The phenotypic classification of the Lipid droplet screen Approximately 7,300 UAS-RNAi lines were used in the screen for regulators of lipid metabolism. A total of 24 candidates were retained and categorized into 5 sub-groups regarding their biological function (right).

Figure 23 -

 23 Figure 23 -Changes in the size of lipid droplet (LD) by Fas RNAi Upper panel (A-C): An RNAi to Fasciclin2 (Fas2) gene (CG3665) randomly induced in a GFP-marked clone shows an increase of LD size (arrows). Lower panel (D-E): An RNAi to Fasciclin3 (from VDRC) in GFP-marked clones induces a weak increase of the size of the LD (arrows). The number of LDs per cell in clones is slightly decreased as compared to the adjacent control cell (GFP negative cells).

Figure 26 -

 26 Figure 26 -A representation of dS6K interactorsAll the candidates selected in the RNAi screen are shown (blue circled). Proteins reported to physically interact with dS6K (pink boxes), and the known direct regulators of dS6K activity (green boxes) are indicated. The candidates which are physically interacting with dS6K (pink color) have been tested; three of them were found to be enhancers (blue circle).

Figure 27 -

 27 Figure 27 -A protein-protein interaction network centered on dS6Kdependent growth

  controls. Specific signals (Fig.28 A,A") were quantified using the Scion imaging software and the dS6K specific values were normalized to the α-Tubulin corresponding values (Fig.28B,B",C,C"). In S2 cells complemented with serum, a moderate increase in dS6K levels was observed upon dsRNA treatment to CG15010, CG10139 and CG1283 but not to CG8318 and CG5279 (Fig.28A,B). The phosphorylation of the T398 residue of dS6K (P-dS6K) was strongly increased under treatment of dsRNA to CG10139, CG1283 and CG8318, whereas it was decreased under treatment of dsRNA to CG15010 (Fig.28A,C). Similar western-blotting assay was performed in serum-deprived S2 cells. In this setting, a clear increase in dS6K levels (Fig.28A",B") concurrent to a decrease in T398 phosphorylation (Fig.28A",C") were observed in S2 cells treated with dsRNA to CG15010. However, none of the other dsRNA produced a noticeable effect on either dS6K levels or T398 phosphorylation. The weak variations that can be observed were not reproduced in other experiments (data not shown) and therefore, are likely unspecific. When selected through the dS6K modifier screen, the RNAi to CG12819, CG11081 and CG1233 were classified as strong but not extreme enhancers. Unexpectedly, none of the corresponding dsRNA when added to S2 cells produced a clear reproducible effect (Fig 28 and data not shown). Altogether, these findings suggest that our screen clearly identifies one gene product (CG15010), which potentially regulates dS6K levels, and 3 gene products (CG1283, CG8318, CG10139), which play on T398 phosphorylation and subsequently on dS6K activity in serum stimulated S2 cells. This further reveals that relevant modifiers for dS6K-dependent growth could be identified following extreme enhancement of the sensitized bent-down wing phenotype.

Figure 28 -

 28 Figure 28 -Western blot analysis to the extracts of S2 cells for dS6K and P-dS6K Left panel: (A) Western-blotting with S2 Cells extracts grown on serum, using antibody specific to dS6K (top), phospho-T398 residue of dS6K (middle) and α-Tubulin (bottom). S2 Cells were treated with dsRNAs to extreme enhancers (CG1283, CG5279, CG8318, CG10139, CG15010) and few strong enhancers (CG12819, CG11081, CG1233). (B) Quantification of the intensity of the bands indicates that dS6K levels are mildly increased with RNAi to CG15010, CG10139 and CG1283. (C) Quantification of the intensity of the bands indicates that phosphorylated dS6K is significantly increased with RNAi to CG10139, CG1283 and CG8318.

  named as Neurofibromin 1 (NF1). It is described as a tumor suppressor, whose mutation also induces neurological disabilities in patient. The Nf1 gene encodes a big protein of almost 3000 amino acids, which functions as a guanosine triphosphatase (GTPase) activating protein for Ras (Ras-GAP), stimulating the intrinsic activity of Ras-GTPase thereby inhibiting the biological activation of Ras. The protein structure of NF1 indicates that it contains two conserved domains: a Sec14p-like lipid binding domain spanning amino acids 1612-1758 in drosophila and 1583-1729 in mouse; and a

Figure 30 -

 30 Figure 30 -Sequence analysis of CG1283 (Cdep) (A) Protein domain structure of CG1283 (Cdep) from Drosophila (top) and mouse (bottom). (B) Amino acid comparison shows a 37.2 % of identity between Drosophila and Mus musculus proteins.

Figure 32 -

 32 Figure 32 -Genetic interaction at the wing between dS6K and ago RNAi The bending down of the adult wing due to dS6K overexpression in the dorsal compartment of the developing wing disc (A) is enhanced by co-induction of an RNAi to archipelago (ago) at 25°C (compare B to C and D to E) and 29°C (compare F to G and H to I). Two UAS-RNAi lines to ago were tested; both of these lines cause a benddown phenotype when co-induced with dS6K in the dorsal compartment of the developing wing using ap-Gal4 driver (C, G, E and I). Notably, the ago RNAi itself seldom induces a slight bending down phenotype (B, F D and H).

Figure 33 -

 33 Figure 33 -Efficiency of ago RNAi through quantitative PCR Quantification of the RNAi suppression on ago expression levels using two ubiquitous

Fig. 12 &

 12 Fig. 12 & Fig. 31). Extensive Y2H assay utilization of the BioGrid source indicates that Ago interacts with several protein targets including Cyclin E, dMyc, dS6K, RpS3, Hippo (Hpo), Supercoiling factor (Scf), Trachealess (Trh), Dysfusion (Dys), Plex A

Figure 34 -

 34 Figure 34 -The Ago substrates share a putative interactive motif Amino acid comparison of Ago substrate proteins reveals that a single high-affinity phospho-epitope is well conserved. Note that some of the target proteins display multiple phospho-epitope sites. The central S/T residue marked by an arrowhead, followed by a proline (P) residue and a conserved S/T residue at position +4 are represented. Two candidates selected during screen which physically interact with Ago, show this conserved S/T site (Plex A, CG2165). A well conserved S/T site is also present in Drosophila S6 kinase (dS6K).

Figure 35 -

 35 Figure 35 -Genetic interaction between ago RNAi and different variants of dS6K Upper Panel: Comparison of dS6K and mS6K1 showing the conserved T and S residues critical for kinase activation. Specific amino acid substitutions are indicated by arrows. The C-terminal (C), linker (L), autoinhibitory (A) and N-terminal (N)

  Lower panel: Using the ap-Gal4 driver, the dS6K variants dS6K TE (A,B,G,H), UAS-dS6K STDE (C,D,I,J), UAS-dS6K STDETE (E,F,K,L) were overexpressed either alone (A,C,E,G,I,K) or in combination with ago RNAi (B,D,F,H,J,L). Males (left panel) and females (right panels) for all the phenotypes are shown separately.

Figure 37 -

 37 Figure 37 -A representation of the eye/thorax ration between dS6K and ago mutantsGraphical representation of the mean value of eye size normalized to the corresponding thorax; at least 15 individual females were used for each genotype. Both ago alleles do not modify eye size, either alone or in combination with dS6K mutation. In contrast, in combination with the H99 deficiency, ago 1 and to some extent ago 3 mutations provoke an increase of eye size. The effect is clearly suppressed by the dS6K mutation showing; thereby that dS6K is epistatic to ago.

Figure 38 -

 38 Figure 38 -Immunostaining to dS6K in wing imaginal disc of the 3 rd instar larvae Driving UAS-dS6K with the ap-Gal4 driver strongly increases dS6K levels in the dorsal compartment of the wing imaginal disc (right panel: left B). An RNAi to ago does not modify neither endogenous nor over-expressed dS6K levels (left panel: left C & left D). A nuclear staining with DAPI is also shown (right A, B, C and D).

Figure 39 -

 39 Figure 39 -dS6K expression level and flip-out clones Upper panel (A-C): Immunostaining to dS6K (B) in the ring gland of the 3 rd instar larvae containing GFP-marked clones (A) expressing dS6K RNAi . The dS6K expression level is significantly reduced in clones (GFP positive cells) compared to neighboring control cells (GFP negative cells).

Figure 40 -

 40 Figure 40 -dS6K expression level in ago mutant clones (A-C): dS6K levels (B) in a wing imaginal disc are not significantly modified in ago 1 mutant clones labelled by the lack of GFP (A); (C) is a merged image. (D-F): loss of dS6K staining (arrows in E) in an ago 1 mutant clone (arrows in D) within a salivary gland; (F) is a merged image. (G-I): mild reduction of dS6K staining (arrow in H) and nucleus disruption (arrow in I) in an ago 1 mutant clone (G) within the larval fat body. (J-L): dS6K level is not significantly modified (arrow in K) in an ago 1 -H99 double mutant clone (arrow in J) within a larval fat body; note that the nucleus is smaller but not disrupted (arrow in L). (M-O): dS6K level (arrow in N) and nucleus (arrow in O) are not significantly modified in an H99 mutant clone (arrow in M) within a larval fat body.

Figure 41 -

 41 Figure 41 -Western blot analysis to dS6K in ago RNAi and ago mutant larval extracts (A) Western blot analysis with specific antibody to dS6K using protein extracts from 3 rd instar larvae. Left panel: Both of the UAS-RNAi lines to ago when ubiquitously induced using either daughterless-Gal4 (da-Gal4) or actin-Gal4 (act-Gal4) driver causes an increase in the dS6K expression levels compared to controls. Right Panel:

  . The Drosophila Cdep physically interacts with Ci (Cubitus interuptus), a component of the Hedgehog signalling, Dof that links together FGF and Ras signalling and Hrs, a predicted Tyrosine kinase substrate (Fig. 27; Giot et al., 2003; flybase.org ). Our findings indicate that in Drosophila imaginal discs, Cdep acts as a negative regulator of dS6K-depednent growth. Cdep is likely an interesting candidate for further investigation. However, to get insight into the role of Cdep regarding cell growth will certainly require an in-depth analysis of its various functional domains.

Table 1 -Drosophila homologs of F-box components and related genes F-box component Homologs and related genes Reported components Proposed substrates

 1

Table 2 -

 2 List of RNAi lines identified in screen for LD sizeThe selected candidate genes identified in the screen for LD size are shown in the table. The putative molecular and biological functions are taken from flybase[START_REF] Anonymous | The FlyBase Consortium). The FlyBase database of the Drosophila genome projects and community literature[END_REF]. Genes are indicated by their name or CG (computational gene) number. The asterisk (line# *) indicates the specific number used by NIG to design each RNAi line for a given gene.

	No.	CG number	Line # *	Gene	Comments
	1	3665		fasciclin 2	learning, memory, nervous system, organ development
	2	14895		Pak 3	receptor signaling protein serine/threonine kinase activity
	3	2647		period	circadian clock, mating, circadian behavior, memory
	4	4979		sxe2	phosphatidylserine phospholipase activity, lipid metabolic process
	5	4581		thiolase	long-chain-3-hydroxyacyl-CoA dehydrogenase activity
	6	12819		slender lobes	Molecular function unknown, involved in nucleolus organization
	7	10792		dunce	synaptic transmission, nervous system, short-term memory
	8	2165		CG2165	calcium-transporting ATPase activity
	9	3522		start1	cholesterol transporter activity, steroid biosynthetic process
	10	10139		CG10139	Unknown function
	11	3961		CG3961	long-chain-fatty-acid-CoA ligase activity, metabolic process
	12	5122		CG5122	carnitine O-acetyltransferase activity, biological process unknown
	13	5904		mRpS31	structural constituent of ribosome, translation
	14	10697		Ddc	amino-acid decarboxylase activity, dopamine and serotonin biosynthesis
	15	3421		RhoGAP93B	protein binding, axon guidance
	16	4630		CG4630	carnitine transporter activity, transmembrane transport
	17	3972		Cyp4g1	electron carrier activity, lipid metabolic process
	18	2101		mRpS35	structural constituent of ribosome, translation
	19	18033		CG18033	transcription repressor activity
	20	3488		α/β hydrolase2	lipase activity, lipid metabolic process
	21	6235		twins	protein phosphatase, regulation of mitosis;, Wnt signaling pathway
	22	10959		CG10959	zinc ion binding; nucleic acid binding, biological process unknown
	23	7111		Rack1	protein kinase C binding, wing disc development, oogenesis
	24	3782		mRpL28	structural constituent of ribosome, translation

Table 3 -List of RNAi lines identified as strong enhancers of dS6K- dependent growth

 3

	No.	CG number	lines (n)	Gene	Comments
	1	1062		CG1062	neurexin binding, phagocytosis, engulfment
	2	1233		CG1233	zinc ion binding, biological process unknown
	3	1283 *		CG1283	actin binding; Rho GEF domain Ezrin/radixin/moesin
	4	2165		CG2165	calcium-transporting ATPase activity
	5	2921		unknown	Domain of unknown function DUF89
	6	3522		start1	cholesterol transporter activity, steroid biosynthetic process
	7	3626		CG3626	phosphatase regulator activity, glycine catabolic process
	8	3909		unknown	G-protein β, WD-40 repeat subgroup
	9	4420		unknown	aspartic-type endopeptidase, proteolysis, ubiquitin domain
	10	4581		Thiolase	long-chain-3-hydroxyacyl-CoA dehydrogenase activity
	11	5084		CG5084	Unknown function
	12	5279 *		Rhodopsin 5	G-protein coupled photoreceptor activity, absorption of visible light
	13	5603		cylindromatosis	ubiquitin-specific protease activity, defense response
	14	5961		unknown	F-box domain, Skp2-like
	15	6917		Estrase-6	carboxylesterase activity, pheromone biosynthesis, courtship behavior
	16	6947		peritrophin-like	peritrophic membrane component; chitin metabolism
	17	7235		Hsp60C	ATPase activity, oogenesis, spermatogenesis
	18	7447		slowdown	EGF-like calcium binding, muscle attachment
	19	8034		unknown	transMb transport; Major facilitator superfamilly MFS-1
	20	8036		CG8036	transketolase activity, cytoplasmic microtubule organization
	21	8318 *		Nf1	Ras GTPase activator activity, regulation of growth
	22	8349		CG8349	cytidine deaminase activity
	23	8411		gcl	protein binding, negative regulation of transcription
	24	8798		CG8798	serine-type endopeptidase activity, proteolysis
	25	8843		sec5	specific transcriptional repressor activity, immune response
	26	8985		DmsR-1	G-protein coupled receptor protein signaling pathway
	27	9104		channel	dentrite morphogenesis
	28	9165		CG9165	hydroxymethylbilane synthase, peptidyl-pyrromethane cofactor linkage
	29	9188		sip2	protein binding, biological process unknown
	30	10139 *		CG10139	Unknown function
	31	11081		plexin A	protein binding, nervous system
	32	12015		RabX6	GTPase activity, small GTPase mediated signal transduction
	33	12026		unknown	Lipoma HMGIC fusion partner-like protein
	34	12455		unknown	CG42817/CG42818
	35	12799		Ubc84D	ubiquitin-protein ligase activity, regulation of protein metabolic process
	36	12819		slender lobes	Molecular function unknown, involved in nucleolus organization
	37	12918		unknown	Protein of unknown function DUF3456

  The adults were further scored for notum defects or more appropriate to Notch signalling for bristle phenotypes. This way, 19.6 % of the RNAi lines produced a visible phenotype. A comparison with a list of genes required for Notch signalling or asymmetric cell division indicates that a huge proportion of the candidates were retained through the screen, ultimately validating the screening strategy. Using secondary assays, they finally identified 6 and 23 novel genes involved in asymmetric cell division and Notch signalling, respectively. In a screen for genes involved for intestinal bacterial infection,Cronin et al., challenged 13,053 RNAi lines representing 10,689 genes using a ubiquitous driver, and found 8.3 % of them as susceptible candidates. Using both a hemocyte-and a gut-specific driver, several of the selected RNAi lines were further analysed. The upmost novelty of their investigation was the identification of the JAK-STAT pathway to control the host

	defense in the gut by regulating epithelial homeostasis through stem cell proliferation
	(Cronin et al., 2009). To identity genes involved in obesity, Pospislik and colleagues
	used a ubiquitous driver to screen 11,594 RNAi lines corresponding to 10,489 distinct
	protein-coding genes. They found 516 RNAi lines (4.5 %) to modify triglyceride
	content, and which were further analysed with the tissue-specific drivers. This study
	revealed hedgehog signalling as an essential determinant for triglyceride accumulation
	in drosophila fat body cells and for the differentiation of white adipocytes in mouse as
	well (Pospislik et al., 2010). In a screen for genes involved in muscle morphogenesis
	and function, Schnorrer and colleagues, challenged a total of 17,759 RNAi lines
	representing 10,461 distinct genes. Using the Mef2-Gal4 muscle-specific driver 2,785
	genes (26.6 %) were identified to produce lethality (20.2 %) or functional muscle
	defect without affecting viability (6.4 %). To identify genes involved in heart function,
	Neely and colleagues screened a total of 8,417 RNAi lines representing 7,061
	drosophila genes conserved in mammals (Schnorrer et al., 2010). Furthermore, after

several repeated screening steps by

Neely et al., 498 genes were identified (7 %)

shown. Note that S6K2 contains a unique proline-rich sequence in its carboxyl-terminus.

The conserved regulatory phosphorylation sites are also indicated (From Radimerski, 2002).

Chapter 2

Chapter 2 -Materials & Methods

-RNAi screen for regulators of steroidogenesis at the ring gland

The transgenic RNAi male flies were generated through P-element insertion carrying a white gene (P{w + , UAS-RNAi}) which gives red color eyes (NIG, Japan). During preparatory step, the UAS-RNAi males were first crossed with double balancer flies (Sp; MKRS/SM5-TM6B). This double balancer line consists of the genetic markers for larvae, pupae and the adults. The red eye colored (Pw + ) UAS-RNAi males with Cyo wing phenotype and carrying tubby marker (UAS-RNAi/ SM5-TM6B) were crossed to virgin Phm-Gal4 flies at 25°C. By using Phm-Gal4 line, Gal4 is expressed under the control of the promoter of phantom gene in the steroidogenic cells of the ring gland which permits the expression of RNAi in these cells. Hence, the individuals of the following cross give rise to the expression of an RNAi restricted to the steroidogenic cells of the ring gland. The UAS-mCD6;GFP was also used simultaneously as a marker to the cells where the Gal4 has been expressed. The Phm-Gal4 line was also balanced by SM5-TM6B; therefore, the heterozygotes Phm-Gal4/ UAS-RNAi would lose the been retain through each strategy. With that respect, the screen at the PG will be described first, since I have been involved only in the preparative steps. I will then present the results of the screen at the fat body and eventually expose the screen for dS6K negative regulators. The last section will concern the detailed studies that we performed for the most relevant negative regulator of dS6K-deopednent growth.

-RNAi screening procedure

To carry out a genome-wide RNAi screen, the drosophila fly stock had been received regularly from the RNAi library of NIG, Japan. The inducible RNAi lines are expressed under UAS/Gal4 system and carry a P-element containing a w + gene as a visible eye marker. Because this P-element (P{w + , UAS-RNAi}) was randomly inserted on chromosomes, different types of eye colours could be observed, i.e., ranging from yellow, orange or red eyes. It is to mention that mostly the P-element was inserted on 2 nd or 3 rd chromosome. Very rarely, we received few RNAi lines with Pelement inserted on X-chromosome or on 4 th chromosome. Out of all, many of the UAS-RNAi lines were homozygous viable and also few were not stably balanced.

Therefore most of the homozygous lethal lines and ill-balanced RNAi lines were established in front of a balancer chromosome.

As the phenotype screened for dS6K-dependent growth was analyzed in adult flies, the inducible RNAi male flies from the NIG stock were directly crossed to the corresponding virgin females, which contain a Gal4 transgene to induce both dS6K and the RNAi simultaneously in the dorsal compartment of the developing wing (Fig. 17). In contrast, the two other screened phenotypes were analyzed in larvae. Therefore, to ascertain for the expression of an RNAi, we made use of a visible larval marker; by crossing first the UAS-RNAi males with virgin females carrying the combined balancers on 2 nd and 3 rd chromosomes (SM5-TM6B). For both screens, the offspring males carrying the UAS-RNAi in front of the combined SM5-TM6B balancers were then crossed to the corresponding virgin females (Fig. 18). This way, the Tubby marker that labelled the combined SM5-TM6B balancers served as a tracker to select those larvae which express the RNAi. (A) A total of 357 enhancers were found and categorized into weak (top), mild (middle) and strong (bottom) enhancers, respectively. (B) The weak enhancers correspond to 1.8%, the mild enhancers to 0.99% and the strong enhancers to 0.454% of the total lines used. About 96.75% of the UAS-RNAi lines did not enhance the dS6K-dependent bending down of the wing.

-Interaction map for dS6K-based growth regulators

Taking advantage of the protein-protein interaction data based on yeast two hybrid (Y2H) assay [START_REF] Formstecher | Protein interaction mapping: a Drosophila case study[END_REF], we analyzed all the gene products of the candidates RNAi lines which were selected as strong enhancers through the screen for dS6K growth modulators. The candidate genes were analyzed using "The bioGRID" (http://thebiogrid.org) and "BOND" (http://bond.unleashedinformatics.com/) webbased sources. Gene products that have been identified in Y2H assay to physically interact with dS6K with high confidence are limited to seven candidates: Sec5 (CG8843), Ago (CG15010), Sucb (CG10622), Pak (CG10295), Cdc37 (CG12019), CG8128 and CG14216. In addition, several genetic, molecular and pharmacological studies in various species including Drosophila melanogaster, have formally demonstrated that PDK1, TOR, Raptor and Rheb act as positive regulators of dS6K activity, whereas the TSC1/2 tumor suppressor act as a repressor [START_REF] Rintelen | PDK1 regulates growth through Akt and S6K in Drosophila[END_REF]Radimerski et al., 2002a;Radimerski et al., 2002b;[START_REF] Kim | mTOR interacts with raptor to form a nutrient-sensitive complex that signals to the cell growth machinery[END_REF][START_REF] Stocker | Rheb is an essential regulator of S6K in controlling cell growth in Drosophila[END_REF][START_REF] Saucedo | Rheb promotes cell growth as a component of the insulin/TOR signaling network[END_REF][START_REF] Zhang | Rheb is a direct target of the tuberous sclerosis tumour suppressor proteins[END_REF]. Figure 26 recapitulates the gene products that have been shown to tightly regulate dS6K activity (green boxes), the seven gene products that physically interact with dS6K in Y2H (pink boxes) and all the candidates selected through our screen which produced a strong enhancement (blueoutlined boxes). Figure 27 represents a physically-interacting molecular network based on Y2H-assay that we were able to draw using available protein-protein interaction information database. For simplicity, the candidates (23 candidates) for which no interaction data is available, have been removed. All the proteins drawn are physically linked, few of these have not been selected through our screen (grey boxes), but they were necessary to establish the physical interacting-network between our selected candidates (Fig 27). In addition to the selected candidates (blue-outlined boxes), some of the genes have been tested in the dS6K modulator screen but their corresponding RNAi did not produce any phenotypic enhancement (black-outlined boxes), although two of these candidates, i.e. RNAi to CG4998 and cact (yellow boxes) produced a weak enhancement (data not shown). Further investigations for all these interactors using the "bioGRID" and "BOND" sources revealed that CG6947 genetically interacts through CG1921 with Ras85D, sina, svp, EGFR, Notch, Dpp and TGF-β (not indicated Upper panel (A-D): GFP-FRT, FRT-dS6K, FRT-H99 and FRT-dS6KH99, respectively.

Middle panel (E-H): FRT-ago 1 , FRT-ago 1 dS6K, ago 1 H99 and FRT-ago 1 dS6KH99, respectively. Lower panel (I-L): FRT-ago 3 , FRT-ago 3 dS6K, ago 3 H99 and FRTago 3 dS6KH99, respectively.

RNAi response (Fig 33 and data not shown). This finding further suggests that Ago does not control dS6K stability in a dose-response manner, but rather in a thresholddependent manner. We therefore switched to the ago mutant alleles, which have been reported to be embryonic lethal [START_REF] Moberg | Archipelago regulates Cyclin E levels in Drosophila and is mutated in human cancer cell lines[END_REF]. Interestingly, few transheterozygous ago 1 /ago 3 mutant larvae could survive until second instar larval stage (data not shown). These larvae were then used to prepare protein extracts and analyzed by Western-blotting with either an antibody to dS6K or a phospho-specific antibody to the phosphorylated T 398 residue of dS6K. As compared to control larvae, dS6K levels were dramatically increased in the transheterozygote ago 1 /ago 3 mutant larvae (Fig. 41B,C). This effect is likely restricted to the genuine inactivated dS6K, since the levels of phosphorylated dS6K at residue T 398 was not increased as compared to an internal control using a Tubulin-specific antibody (Fig. 41D).

Quantification of the specific signals and normalization to the α-Tubulin corresponding values (Fig. 41B) confirms that dS6K levels are increased (Fig. 41C), while T398 phosphorylation is slightly decreased (Fig. 41D). In conclusion, our findings strongly support the notion that Ago regulates dS6K levels. Considering that the E3-ligase complex controls substrate ubiquitination and the subsequent targeting to the proteasome, it is likely that Ago regulates dS6K degradation.

Chapter 4

Chapter 4 -Discussion and Perspectives

-Comparison of genome-wide RNAi screen in Drosophila

During my PhD research work, I used Drosophila as a model system for genome-wide screens. Taking advantage of the inducible UAS-RNAi lines from NIG, three screens have been performed to identify novel regulators of 1) steroidogenesis, 2) lipid metabolism and, 3) dS6K-dependent growth. I participated in the preparative step that was common of the two first screens scored in their F2 progeny, but, in fact, I did not perform the further screening steps of the first one. The third screen was scored in the F1 progeny. Considering the work published by others and the list of candidates identified, I decided to focus on the genes identified in this latter screen to complete my PhD work. In the following section, I will discuss in details the results of the screens for lipid metabolism and dS6K-dependent growth. However, as I was involved in the three distinct genome-wide RNAi screens, which, therefore, gave me an opportunity to compare the number of candidate genes retained through our strategies with the results of genome-wide screens recently published by others. [START_REF] Moberg | Archipelago regulates Cyclin E levels in Drosophila and is mutated in human cancer cell lines[END_REF]. The overgrowth of ago mutant tissue implies that ago mutant cells grow at an accelerated rate in comparison to wild-type cells [START_REF] Moberg | The Drosophila F box protein archipelago regulates dMyc protein levels in vivo[END_REF].

Because Drosophila Cyc E has also been shown to promote S phase entry but not growth [START_REF] Neufeld | Connections between growth and the cell cycle[END_REF], the increased growth of ago cells suggested that there are other ago substrates that promote cell growth [START_REF] Moberg | The Drosophila F box protein archipelago regulates dMyc protein levels in vivo[END_REF]. In addition, it has been shown that both dMyc and Cyc E interact with Ago through a well conserved S/T sites. These findings suggested that the increase of Cyc E and dMyc levels cooperate to promote growth in ago mutant cells [START_REF] Moberg | The Drosophila F box protein archipelago regulates dMyc protein levels in vivo[END_REF][START_REF] Mortimer | The Drosophila F-box protein Archipelago controls levels of the Trachealess transcription factor in the embryonic tracheal system[END_REF].

ago encodes a 1326 amino-acids protein containing an F-box and seven WD repeats through which it interacts with its substrate, thereby regulating the turnover of its targeted protein [START_REF] Skowyra | F-box proteins are receptors that recruit phosphorylated substrates to the SCF ubiquitin-ligase complex[END_REF][START_REF] Mortimer | The Drosophila F-box protein Archipelago controls levels of the Trachealess transcription factor in the embryonic tracheal system[END_REF]). An S/T site followed by proline (P) is present in all of the interactive proteins of Ago including the auto-inhibitory domain of dS6K, suggesting that it is putative Ago target (Fig. 34 & 35). Considering that Ago physically interacts with dS6K in Y2H assay [START_REF] Formstecher | Protein interaction mapping: a Drosophila case study[END_REF], and that ago RNAi was found in our screen to enhance a dS6K-dependent wing phenotype, we further investigated the potential role of Ago in the dS6K degradation. In favor of this hypothesis, we observed that overgrowth of ago mutant tissues requires a functional dS6K and that dS6K levels were dramatically increased in ago mutant larvae. Likewise, an increase of dS6K levels was observed in S2 cells treated with dsRNA to ago, in particular in serum-deprived S2 cells. Unexpectedly, the increase of dS6K levels was accompanied by a decrease of dS6K phosphorylation in both S2 cells and protein extracts of mutant larvae. It will be necessary then to determine whether the molecular processes controlled by dS6K are increased. To address this issue, a mammalian S6 transgene will be combined with ago mutants since the mammalian ribosomal protein S6 is a competent target for dS6k and a phosphospecific antibody is available to monitor S6 phosphorylation. Surprisingly, we were unable to detect an increase of dS6K levels in clonal ago mutant cells of imaginal discs. In endoreplicative tissues an increase of dS6K immuno-staining could be observed in flip-out clones expressing ago RNAi , but not in homozygote mutant clones. It has been earlier reported that Ago regulates growth differentially in different