F Rémy

N Vayssière

F Bazzi

D Mateo

A Sadoun

P Girard

M Mescam

Caroline Fonta

Lifespan cerebral grey matter changes in the marmoset monkey

établissements d'enseignement et de recherche

LIFESPAN CEREBRAL GRAY MATTER CHANGES IN THE MARMOSET MONKEY

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  . Rémy, N. Vayssière, F. Bazzi, D. Mateo, A. Sadoun, P. Girard, M. Mescam, C. Fonta Centre de Recherche Cerveau et Cognition (CerCo) -UMR 5549 -CNRS -Université Paul Sabatier, Toulouse, France Animals & Methods Background MRI Results -Global and regional GM decreases with age Conclusions: relevance of the marmoset model in neural ageing research  Forty-nine marmosets (25 males, 24 females) of various ages, from 6-months to 14-years old, with 21 individuals > 8 years old  3T-MRI high-resolution (0.35 × 0.35 × 0.35 mm 3 ) T1-weighted scans  Scan segmentation and spatial normalisation using the DARTEL processing pipeline (Ashburner, 2007) on SPM12  Assessment of individual intra-cranial volume (ICV) and gray matter (GM) volume within a standard marmoset forebrain mask (Risser et al. 2019)  Whole-brain voxel-based morphometry (VBM) of GM images  The common marmoset Callithrix Jacchus model receives increasing interest in neural ageing research (Tardif, 2019)  Marmoset cognitive ageing parallels human cognitive ageing, with deficits in executive function and short-term memory (Sadoun et al. 2019; Lacreuse et al. 2020)  Characterization of cerebral ageing in marmosets is currently needed References  The total GM volume (normalized by the total ICV for each individual) showed a decrease with age (p = 0.025, with a moderate fit of the data : r = -0.32) males females Linear decreases of GM volume with age were observed (p < 0.001)  in the bilateral frontal cortex (prefrontal and motor regions)  in sub-cortical GM (striatum and pallidum)  In limbic regions (cingulate gyrus and right hippocampus / parahippocampal gyrus)  Due to its short life expectancy (10 years on average), the marmoset model is valuable in ageing biomedical research  Beta-amyloid plaques and hyperphosphorylated tau spontaneously develop in aged marmoset brain (Geula et al. 2002 ; Rodriguez-Callejas et al. 2016)  Marmoset age-related cognitive decline has been evidenced and is variable between individuals  We show here that marmoset brain GM changes occur with age. Changes were mostly observed in anterior brain regions, with a spatial pattern close to the GM atrophy pattern reported in humans  A previous study showed age-related decreases in GM T1 relaxation time (Liu et al. 2011) such as in humans. Here we show that global GM volume tends to decrease with age, also similar to humans (Good et al. 2001)  Spatial pattern of GM atrophy with age was consistent with the pattern observed in healthy human ageing (Good et al. 2001 ; Raz et al. 2010)  GM atrophy in the prefrontal cortex and anterior cingulate gyrus is consistent with deficit in executive function observed from middle age (Sadoun et al. 2019)  Ashburner J, NeuroImage 38: 95-113, 2007  Geula C et al., Acta Neuropathologica 103: 48-58, 2002  Good et al., NeuroImage 14: 21-36, 2001  Lacreuse A et al., Phil. Trans. R. Soc. B 375: 20190618, 2020  Liu et al., NeuroImage 56: 1154-1163, 2011  Raz et al., NeuroImage 51: 501-511, 2010  Risser L et al., Brain Structure and Function 2019  Rodriguez-Callejas et al., Frontiers in Aging Neuroscience 8: 315, 2016  Sadoun et al., Neurobiology of Aging 74: 1-14, 2019  Tardif S, Am. J Primatol. E22912, 2019