Dr Morgan Camier

Dr Kalyan Dhara

Dr Marion Tisseraud

Dr Alexis Tabey

Dr Julien Gicquiaud

Dr Nicolas Fantozzi

Dr Nancy Leon Janampa

Thifanie Christine

Baptiste Abadie

Laura Bourmeyster

Yannick Landis

Prof Laurent Elkaim

A Fournet

A Rojas De Arias

M E Ferreira

H Nakayama

S Torres De Ortiz

A Schinini

M Samudio

N Vera

This research work is dedicated to Chimamanda, Munachisom,

My sincere gratitude goes to the families of Sir Chuks and Lady Patience Okoronkwo; Honourable Chukwemeka Nwajiuba; Prof Chinyere A. Nwajiuba; Venerable Amos and Mrs. Lois Chukwuocha; Rt. Rev'd Ken C. and Mrs Afoma Ifemene and Most Rev'd Dr David O.C. and Mrs Blessing Onuoha who always aimed at bringing out the potential in me, my God will reward you all.

May God cause His face to shine upon our path and be gracious onto us always, CHINEKE GOZIE ONYE OBULA!!! To God be the glory, for His goodness and mercies endures forever! RÉSUMÉ BisBenzyl-tetrahydroIsoQuinolin (BBIQ) est une famille très importante de molécules, de par leur nombre, mais aussi du point de vue de leurs activités pharmacologiques. La trilobine et l'isotrilobine (son homologue N-méthylé) font partie de cette famille des BBIQ qui sont classées en différentes sous-classes, ces composés sont des BBIQ de type XXIII, i.e. avec trois ponts diaryléthers, reliant les deux benzylisoquinolines d'une manière tête-à-tête, avec un système dibenzodioxiranne. Initiallement, la trilobine montrait des activités antiparasitaires (leishmania sp., trypanosoma cruzi, plasmodium falciparum, . . .), puis des études avaient montré d'autres intérêts avec des activités biologiques anti-protozoaires, anti-inflammatoire et anti-tumorale. 1 Plus récemment, ils ont été évalués en tant que régulateur épigénétique avec des activités d'inhibition des méthyltransférases d'ADN (DNMTs). 2 Ces produits naturels ont été extraits des parties aériennes de Cocculus hirsutus, (Menispermaceae) 3 et des travaux de dérivatization ont été entrepris, mais sans amélioration notable des activités biologiques. La synthèse totale de la trilobine a été rapportée en 1976 [START_REF]-O-Piv-allylbenzene (315) To a solution of ferrous sulphate (2.58 g (9.27 mmol, 9 eq.) and allyl bromide[END_REF] , mais un grand nombre de challenges persistent pour améliorer cette synthèse ayant un grand nombre d'étapes et de très faibles rendements.

Le but de cette thèse est d'étudier une nouvelle voie de synthèse, courte, efficace et flexible pour permettre la préparation de molécules naturelles mais également d'analogues, par modifications des substituants, de la taille et la nature des cycles.

La synthèse a été menée avec deux étapes clés : l'une est la synthèse des isoquinolines par réaction Pictet-Spengler ou Bischler-Napielralski, en utilisant la dopamine et des acides phénylacétique convenablement substitués. Le but est de protéger les hydroxyles et l'amine, et de permettre la cyclisation en tetrahydroisoquinoline par réaction de Pictet-Spengler. La seconde réaction-clé est la formation de parties diaryléthers réalisée par des réactions de type Ullmann catalysées par des sels de cuivre comme Cu(CH3CN)4PF6. Le système dibenzodioxiranne a été formé avec K2CO3 dans le DMF, avec un excellent rendement.

Le but de ces travaux de recherche est de développer une voie de synthèse, efficace, avec le moins d'étapes possible pour ces structures dimériques et permettre l'accès à des substances biologiquement actives, et à des analogues par modificationsdes substituants des noyaux aromatiques, et par la taille et la nature du macrocycle.

RÉSUMÉ (in english)

BisBenzyl-tetrahydroIsoQuinoline (BBIQ) is a family of very important molecules with a great potency from a pharmacological point of view. So, the Trilobine and Isotrilobine (its Nmethylated homologue) take part to this family of BBIQ, and are classified in differents sub-class.

These two are BBIQ type XXIII, i.e. with three ethereal bonds linking the aromatic rings in a headto-head manner, with a dioxiran system. Initially, trilobine has shown antiparasitic activities (leishmania sp., trypanosoma cruzi, plasmodium falciparum, . . .) then, studies have shown that has the ability to demonstrate other biologicals activities in anti-protozoal, anti-inflammatory and, antitumour 1 . More recently, they were evaluated as epigenetic regulator with inhibition of DNA methyltransferase (DNMTs) inhibitors 2 .

These natural alkaloids, have been extracted from aerial parts of Cocculus hirsutus (menispermaceae), 3 and derivatisation programhas been already engaged for preparation of derivatives, but without really improvement of the biological activities. The total synthesis of trilobine was first reported in 1976 [START_REF]-O-Piv-allylbenzene (315) To a solution of ferrous sulphate (2.58 g (9.27 mmol, 9 eq.) and allyl bromide[END_REF] and several number of challenges and some disadvantages still exist such as tedious reaction conditions, low yields, and multiple sequences.

This synthetic route will use two main key steps, the tetrahydroisoquinoline synthesis using Pictet-Spengler or Bischler-Napieralski reaction, and the formation of diaryl etheral bonds by metallocatalysed Ullman or Ullman-type coupling reaction, catalyzed by a copper salt such as Cu(MeCN)4PF6. However, we also engaged in the formation of dibenzo-p-dioxine compound with K2CO3/ DMF instead of metallocatalyzed salt and it gave excellent yield. The target in this research is to develop a synthetic strategy that is efficient in having a short route for dimeric structure and provide access to diverse bioactive principles and its analogues.

The goal of this research was geared towards the study of a new synthetic route, short, efficient, and flexible, which will enable us to prepare the natural molecules with different analogues on it through the modification of the aromatic substituents, and the size and nature of the macrocycle.

ABBREVIATIONS

PREAMBLE

The project of total synthesis of trilobine and isotrilobine was engaged some years ago, in Toulouse in the former CNRS and the Institut de Recherche Pierre Fabre joint unit USR 3388 ETaC, Epigenetics, and Targeting of Cancer. Working for one part in isolation and characterization of natural products from natural resources and for another part in chemical modifications of these natural substances, the main goal of this unit was to developed new biological actives molecules (mainly for Oncology or Central Nervous System) based on Natural Substances. Several High Throughout Screening campaigns allowed to identify "hits" active on different pharmaceutical targets. One of this campaign looked for identifying epigenetic modulators, as inhibitors of DNA methyltransferase. During this campaign, trilobine and its homologue, the isotrilobine, (Figure 1) were found to have interesting activity towards these methyltransferases, and it was decided to develop a project for the preparation of these molecules by total synthesis, in addition to a program of derivatization by semisynthesis from natural trilobine. Results for the semisynthesis program was already published2. The total synthesis program was initiated with the work of Alexis Prieto 5 , a master student. For our part, trilobine, isotrilobine and several other related alkaloids were isolated from roots of a Menispermaceae, Cocculus hirsutus, with a yield of 100 mg per kilo (and 125 mg per kilo for isotrilobine).

Due to low possibilities for modulation anchorage, only one nitrogen is free for modifications, the derivatization program failed to improve the potency of this series, and the total synthesis becomes more and more relevant for the obtention of biologically active molecules from natural substances.

In 2016, the collaboration between Pierre Fabre Laboratories and the CNRS took a new direction and some projects dealing with chemical modulation of naturals substances, including "Total synthesis of Trilobine" were moved to Bordeaux, with the move of Frédéric CANTAGREL from Toulouse to Institut des Sciences Moléculaire, at Université de Bordeaux.

INTRODUCTION

A. Generality on medicinal plants

Natural products are any substance(s) produced by a living organism that are found in nature. They have pharmacological or biological activities because humans relied much on it for their basic needs either as poison, medicine, or food throughout their existence. Plants play an important role in natural products and they have been characterized according to their (primary or secondary) metabolites [START_REF] Deus | [END_REF] .

Medicinal plants are of great value in the field of treatment and cure of diseases in mankind, as most herbs demonstrates great versatility in the health care system. It has now been universally accepted that herbal medicines are used in curing many complex diseases. Especially, the developing countries employ their indigenous herbs practices to cure their prevailing illness based on its method of applications 7 .

The use of medicinal plants for the benefit of mankind has long been sought and it is on the increase day by day. However, the taxonomy of a plant is very essential to understand its communities based on origin and how they are related to each other [START_REF] Wink | [END_REF] . Their exploitation has been increasing, once they are recognized as rich sources of natural products with a huge variety of chemical constituents and the most developed specimens of the plant kingdom help to play an important role in the benefit of mankind's health system 9 .

The idea of morphological classification and chemical profile of plants is considered a useful tool towards the characterization and determination of plant families across their chemotaxonomic profile 10 . Secondary metabolites are compounds that perform non-essential functions in plants and are used for protection and defense against predators and pathogens. They are compounds of restricted occurrence and are very useful in chemotaxonomic classification 11 .

Recently, the increase in the studies related to chemotaxonomy is mainly due to the advance of analytical techniques that can detect even traces of chemical compounds and the findings related to this chemotaxonomy are useful for taxonomist, phytochemists, and pharmacologists to solve possible problems.

B. About alkaloids

In 1819, a German pharmacist, Carl Friedrich Wilhelm. Meiner, laid the foundation of alkaloids chemistry, even if alkaloids-containing plants were known since the early time of Ancient History, attested by Sumerian cuneiform tablets. The name comes from the Medieval Latin alkali, himself coming from the Arabic القالي (al qaly which mean "soda"), and the Greek suffix ε ̓͂ ιδος ("oid" which means "with the form of").

Meiner defined it as basic nitrogenous compound of plant origin, which has a complex molecular structure and many pharmacological activities. By the same time, Pierre-Joseph Pelletier isolated alkaloids (with Joseph Bienaimé Caventou) like Strychnino -(1818)(Figure 2-page 14), quinine 4 (1820) (Figure 2-page 14), caffeine 10 (1820)( Figure 4 page 14) and he defined alkaloids as a cyclic compound containing nitrogen in negative oxidation state which is limited in distribution in the living organism.

The first total synthesis of an alkaloid was the work of Albert Ladenburg in his laboratory, in Kiel, in 1886. Before he had already prepared coniine 11 (in his racemic form -Figure 4 page 14) from acetaldehyde and 2-methylpyridine 12 , and redefined alkaloids as natural plant compounds that have basic characters and contain at least one nitrogen atom in a heterocyclic ring and they are having biological activities.

Although it is still difficult to define alkaloids, so alkaloids are biologically active basic nitrogenous secondary metabolite obtained from natural resources, mostly optically active and possessing nitrogen, often within a heterocyclic ring, as their structural unit 13 . The "official" definition given by IUPAC is: "Basic nitrogen compounds (mostly heterocyclic) occurring mostly in the plant kingdom (but not excluding those of animal origin). Amino acids, peptides, proteins, nucleotides, nucleic acids, amino sugars and antibiotics are not normally regarded as alkaloids. By extension, certain neutral compounds biogenetically related to basic alkaloids are included." 14 Alkaloids can be localized in leaves, seeds, or roots of the plants which vary widely depending on the species and its organ, the vegetation period of the plant and the environmental conditions such as climate, soil, humidity and others. The natural organism with single alkaloids is rare in instances because most of them come in mixture form with a wide varies of components as most plants are usually found as salts of various organic and mineral acids. 15 Probably, over 10000 compounds of alkaloids properties are known to comprise the largest single class of plants secondary metabolites (more and more are often called specific metabolites, in reference to their specific actions in the plant). They possess important biological properties and with the advances in technology, the structural diversity of this secondary/specific metabolites have become the target of a constant investigation by researchers around the globe. 16

Extraction of alkaloids:

Extraction of bioactive alkaloids from plant is one of the fundamental and crucial step before any applications. Nowadays, conventional organic solvents (e.g. methanol, alcohol, ethyl acetate, acetone, and chloroform) have been widely used in the extraction of alkaloids from plant materials. Nonetheless, most of these solvents show many intrinsic drawbacks such as environmental pollution, high toxicity and cost 17 . Although the extraction media of the natural product proved to be of novel high-efficiency, which is of great interest in the type of bioactive compound extracted as it may help to prevent various diseases because they possess important biological properties like antiinflammation, anti-depression, anti-oxidant, anti-diabetic 18 and so on. For example, most of the bisbenzyltetrahydroisoquinoline alkaloids (BBIQs) isolated from Stephania tetrandra, have recently been found to exert inhibiting release of histamine, and reducing myocardial oxygen consumption 19 . For our part, extraction of alkaloids (BBIQs and THIQs) was performed from Cocculus hirsutus by Long et al. Error! Bookmark not defined.

Classification of alkaloids

The chemical, pharmacological and botanical properties must all be considered in classifying a compound as an alkaloid since the plant species are in the same family often produce bases with a similar biogenetic structure that are convenient with their origin and structure type. Based on this knowledge and speculation, alkaloids are grouped into three main classes 20,21 ; a. True alkaloids True alkaloids are compounds in which the nitrogen-containing heterocyclic system is derived from amino acid and are highly active substances with biological activity even in low doses. They appear as a white solid with a bitter taste with some exceptions like nicotine 3, which is a brown liquid, and the alkaloids are water-soluble salts. True alkaloids occur in the plant either as a free-state, salts or N-oxide. They occur in a limited number of species and families in which decarboxylated amino acid is condensed with a non-nitrogenous structural moiety. Examples of true alkaloids includes biologically active alkaloids as quinine 4, strychnine 5, morphine 6 and others (Figure 2).

b. Proto alkaloids

Proto alkaloids are like true alkaloids, derived from amino acid but they do not contain any heterocyclic system. They are those with a closed ring, being perfect but structurally simple alkaloids in the minority of all alkaloids for example dopamine 7 ephedrine 8, mescaline 9, and others.

Alkaloids in nature

Alkaloids are basic in nature and form water soluble salts with mineral acids. The degree of basicity varies considerably, depending on the structure of the molecules and the presence or location of the functional groups as most of the alkaloids are crystalline solids and are bitter in taste. Alkaloids are colourless, crystalline compounds but some exceptions are in liquid form like the nicotine. Nicotine 3 is a hygroscopic oil liquid and miscible with water in its base as well as it salt form. It possesses two nitrogenous ring systems: one is pyridine and the other is a pyrrolidine ring system with a potent nerve poison. In lower concentration, nicotine is a stimulant i.e. it increases activity, alertness, and memory, which is one of the factors that contribute to the dependence-forming properties of tobacco smoking. While high doses can cause nausea and vomiting. However, the following table shows different major types of alkaloids, their generic skeletons, and specific examples 22,23. .

C. The BBIQ alkaloids

The THIQ alkaloids

Simple isoquinoline alkaloids are known to have only one aromatic nucleus and no other cyclic structure except a methylenedioxy substituent, which is mostly derived from only one aromatic amino acid residue like tyrosine moiety. Some families like the Cactaceae, Chenopodiaceae and Fabaceae are known to produce simple isoquinoline alkaloids24 contrary to Menispermaceae, Monimiaceae where the majority of isoquinoline alkaloids are dimerics.

However, the formation of isoquinoline 13 alkaloids usually involves the amine and aldehyde in which several compounds can serve as precursors in specific instances, while the tetrahydroisoquinoline 14 alkaloids are widespread across almost all the families that possess isoquinoline alkaloids25 (Figure 5). Some of the benzylisoquinoline 15 like papaverine and benzyltetrahydroisoquinoline alkaloids have gained importance as clinical agents. The majority of benzyltetrahydroisoquinoline 16 alkaloids are isolated from plants with oxygen substituents in 6-and 7-positions, and also in 8-, 11and 12-positions. This is a characteristic of a large group of isoquinolines that arise biogenetically from benzyltetrahydroisoquinoline precursors26 . Benzyltetrahydroisoquinoline alkaloids have been observed in plants from the families Berberidaecae, Fumariaceae, Papaveraceae, Ranunculaceae, and Rhamnaceae27 (Figure 6). The bisbenzyltetrahydroisoquinoline alkaloids are structurally constructed from two monomeric benzyltetrahydroisoquinoline units (THIQ) joined by one or more ether bridges. The substituent on the aromatic rings may be hydroxyl (OH) or methoxy (OMe) or methylenedioxy (OCH2O) groups. The complexity of bisbenzyltetrahydroisoquinoline alkaloids is the two asymmetric centres, which can be in R or S configuration. However, these have been a challenge in the identification of new bisbenzyltetrahydroisoquinoline given to the degree of variation of structural features differing in oxidation state or the degree of substitution of the two nitrogen atom (imine, NH, NMe, or quaternary amine) 2829,26 (Figure 7). However, over hundreds of bisbenzyltetrahydroisoquinoline alkaloids are known and the two benzyltetrahydroisoquinoline units may be bonded together by one, two or three diaryl ether linkages. When only one diaryl ether linkage is present, the bond involved tail-to-tail or head-to-tail coupling but not head-to-head coupling. If the linkage is two or three diaryl ether, the benzyltetrahydroisoquinoline units can be bonded either head-to-head or head-to-tail, which makes the diversity in the structures of bisbenzyltetrahydroisoquinoline alkaloids, coupled with their known or potential pharmacological activities that have stimulated substantial interest in their synthesis. The consideration of the head and tail nomination will be explained in the next part.

b. Numerotation

The bisbenzyltetrahydroisoquinoline alkaloids are structurally constructed of two monomeric benzyltetrahydroisoqunoline unit joined by one or more ether bridges. More so the direct carbon-tocarbon biphenyl and methylenedioxy linkages are also found in some of these units. The bisbenzyltetrahydroisoquinolines have six rings (A, B, C, A', B', C') which numbers the skeleton of the alkaloid structure. The tetrahydroisoquinoline portion is considered as the 'head' of the monomer because they contain rings A and B. as the ring A tends to possess an unsaturated aromatic ring system with two, three or more oxygenated substituents, and ring B possess secondary, tertiary or quaternary amine which sometimes varies with both saturated and unsaturated aromatic system. While the benzyl portion is considered as 'the tail' of the monomer with the ring C because it has only one ring with one or two substituents. The numbering of bisbenzyltetrahydroisoquinoline skeleton is generally represented by the structure, shown in Figure 8.30 Biosynthesis is the experimental study of the formation of a secondary metabolite which starts from the hypothetical speculation on the precursor product and this tends to relate with the biosynthetic pathway. Most alkaloids of Menispermaceae are isoquinoline type derived from benzyltetrahydroisoquinoline which originated from tyrosine31 . Over the past decades, biogenesis has proved to be a very interesting and fruitful area of organic chemistry. However, the Menispermaceae family have the isoquinoline type that form the tetrahydroisoquinoline alkaloids which originated from the tyrosine32 .

Isoquinoline alkaloids are derived from benzyltetrahydroisoquinoline. The initial reaction in benzyltetrahydroisoquinoline biosynthesis is a condensation of two aromatic units; both derived from tyrosine or dopamine, and hydroxyphenylacetaldehyde. However, Schumacher et al., 33 proved by the discovery of a novel enzyme named norlaudanosoline synthase, that route I is a real pathway of benzyltetrahydroisoquinoline biosynthesis and that the previously postulated intermediate norlaudanosoline-1-carboxylic 27 is probably an artefact (Scheme 1).

In a more modified step, when (S)-tyrosine 22 involved the enzyme of decarboxylase it forms tyramine 29 which was hydrolyase at meta-position to form the dopamine 7 compound. On the other hand, the same tyrosine 22, lyase to forms the p-hydroxyphenyl pyruvate 30, which was further decarboxylase to afford the p-hydroxyphenyl aldehyde 31. The experimental precursor and cell cultures showed that condensation between dopamine 7 and p-hydroxyphenyl aldehyde 31 form the first alkaloid intermediate (S)-norcoclaurine 32 which is an intermediate of bisbenzyltetrahydroisoquinoline isoquinolinealkaloids 3431 (Scheme 2). The (R)-norcoclaurine 32 can be changed to (R)-coclaurine 33b simple by O-methylation of the hydroxyl group at position 6. The (R)-coclaurine 33b can undergo triple methylation at secondary amine, 7' and 12'-positions of the aromatic system to form (O)-methylarmepavine 36, and when hydrolyzed, compound 37 is obtained. When two units of tetrahydroisoquinoline moieties of 35 and 37 are combined through an oxidative reaction, there's a formation of diaryl ether bridges like dauricine 38. However, the dimerization of one, two or three oxidative couplings can form the bisbenzyltetrahydroisoquinoline alkaloids and these sequences are often initiated from tyrosine 31 (Scheme 3). Scheme 3 : Biosynthesis of dauricine from (R)-norcoclaurine 2. Dimerization of THIQ a. Dimerization process Bisbenzyltetrahydroisoquinoline (BBIQs) alkaloids belong to the very large isoquinoline alkaloid family consisting of two benzyltetrahydroisoquinoline parts linked through diphenyl ether, benzyl phenyl ether, or biphenyl bonds 26 . The bisbenzylisoquinoline alkaloids are mostly found in the Menispermaceae, Berberidaceae, Lauraceae, and Ranunculaceae families and these plants preferably grow in tropical and subtropical regions. The most prodigious source of these alkaloids is plants of Menispermaceae family which the genus Cyclea is known to be the richest source of alkaloids isolated 29,37 . So far, over 500 benzyltetrahydroisoquinoline dimers are been characterized and the alkaloids that occur in a plant of menispermaceae family are been subjected to several reviews from their botanical point of precept 38 . The position and the number of ether linkages in the molecule have served as a convenient criteria for the classification of these alkaloids into subgroups. Based on the differences of aromatic oxygen substituents, the number of linkages, types, and ether bridges play an important role in classifying the bisbenzyltetrahydroisoquinoline alkaloids. To understand the clarity of atom on bisbenzyltetrahydroisoquinoline alkaloids, the priority is referred to as the hemisphere which is separated by a hyphen such that each diaryl ether linkage has a symbol of *, + and # symbols. There is a need to expatiate the tetrahydro-, dihydro-or fully aromatic isoquinoline moiety even when it does not give more information about the stereochemistry of the stereogenic centre at C-1 and C-1' or pattern with N or O-methylation. The more highly oxygenated benzyltetrahydroisoquinoline half constitutes the right-hand side C-1 of the dimer and is listed first while the prime (C-1') constitutes the less oxygenated molecule on the left-hand side. each half of the dimer concerning its oxygenation pattern on both the type(s) and position of the coupled compound 30,31 ,39 .

The Shamma-Moniot nomenclature

Regards to the number of BBIQs described, and moreover regards to the diversity of their structures, it became pertinent to create a specific classification for these dimeric compounds. In 1976, Maurice Shamma and Jérome Moniot have presented a classification of these BisBenzyltetrahydroIsoQuinoline, and this classification is still ongoing, nowadays. After them, several authors and first of all Paul L. Schiff Jr. have enriched it and many reviews were published Error! Bookmark not defined., 40, 41 A recent and almost exhaustive description of all these types was published in 2019 by Weber and Opatz. 35

a. Etheral connection

The simplest link between two benzyltetrahydroisoquinoline, is an ether bond, so the first groups of this subdivision (type I to type III) described BBIQ with one ethereal link, and all of them are connected by the tail, i.e. by the benzyl moiety, and never by the isoquinolines moiety. The differentiation between these isoquinolines is the oxidation level of aromatic rings and the position of the ethereal bond. Thus, type VI to XVII are dedicated to BBIQ with two ether bridges, one which link the two benzyl moiety and the second bridge connecting the two isoquinolines. Finally, type XXIII, XXIV, and XXVIII, for BBIQ contain 3 etheral bridges, with two for the connection between the isoquinolines. As molecules are isolated and characterized, this classification is continuing to be incremented, this is the reason why the continuum if the numeration is not in the following. Usually, the numeration begins with molecules possessing a single bond to finish with molecules which isoquinoline moiety is connected with three bridges. It is also for that reason, that sometimes, authors create type Ia to a make distinction with normal type I. The difference is just an additional hydroxyl group in position 5, but one more time because the first compound with this over oxidation was isolated after the establishment of the classification, it was not possible to change numeration, and the creation of an extension of type I appears to be the simplest. This is also the same case for type XII/XIIa, for type XXIII/XXIIIa. When compounds with and without oxidation in this position 5 were already discovered before 1976, two different types were created and it is the case for type VI and type VII (see Table 2). As already mentioned, numeration of subgroups type of BBIQ Shamma-Moniot classification depends on the order of isolation and characterization of natural BBIQ and not with any relation with the structure. Thus, concerning the third group where THIQ is connected with one no-ethereal bridge but an aryl-aryl one, compounds with this single carbon-carbon bond are classified as type XXVII, while compound as cordobine with one aryl-aryl bond and an ethereal bridge are classified as type IV. All compounds classified in type XXVII, i.e. pisopowine and analogues, were isolated from Popowia pisocarpa, an Annonaceae, by Cavé et al. in 1986, and so 10 years after the introduction of the classification by Shamma and Moniot, for that reason these molecules with a simple link are classified with a "high" number. This aryl-aryl link only occurs for the benzyl moiety and never, up to now, to connect the isoquinoline moieties Type XVIII and XIX are dealing with compounds which possesses a dioxin system in addition to this aryl-aryl bond (Table 3). The dimerization process of two THIQ can also lead to other types of dimeric compounds, by a head-to-tail junction. Thus, a tetrahydroisoquinoline of the first THIQ is connected to the benzyl of the second one, and not to the second isoquinoline moiety. This is especially the case for a very wellknown family of natural poisons, the curare. In fact, curare is a generic name for highly poisonous substances mainly extracted from Menispermaceae Chondodendron tomentosum and from the Loganiaceae Strychnos toxifera. The molecule known as curare is curine, which is a type XXI BBIQ alkaloid, and it is used in the clinic as its ammonium salt under the name of Tubocurarine. One specificity of this subgroup, is the presence in some types of a phenyl-benzyl ether bridge instead of a classical phenyl-phenyl ethereal bridge, as in type XXII, XXIIa and XXVI (see table Table 4). 12). In 2018, Menéndez-Perdomo and Facchini had isolated a head-to-tail BBIQ, Nelumborine 67 43 , classified in type XXXI, with a single aryl-aryl bond, related to compound 64 described by Giannis but without the ethereal link (Figure 13). For the moment, some newly isolated compounds are not classed in subgroups BBIQs type and even were not to be named (it is the case for compounds 62-66 isolated by Giannis et al.) but the community will probably create a new type, as it is done regularly. 42 G.R. Malca Garcia, L. Hennig, I.V. Shelukhina, D.S. Kudryavtsev, R.W. Bussmann, V.I. Tselin, A. Giannis ; J. Nat. Prod. 2015, 78 (11), 2537 -2544. 43 I.M. Menéndez-Perdomo, P.J. Facchini, Molecules, 2018, 23 (11), 2899 -2915. Since the earliest commercial drug preparation, natural product chemist and pharmacognosists have been interested in the bisbenzyltetrahydroisoquinoline alkaloids because of their diverse formulations and varied pharmacological effects. The Shamma-Moniot reported the chemical aspects of this field and has intensified the long-felt need for a glossary of these alkaloids containing all information necessary for a natural product chemist searching for new BBIQ alkaloids. However, the BBIQ alkaloids are built up of two benzyltetrahydroisoquinoline unit linked by ether bridges, methylenedioxy bridges, or direct carbon-to-carbon bonds.

Biological activity / pharmacology of BBIQ

The number of publications concerning the pharmacological activities of bisbenzyltetrahydroisoquinoline alkaloids has largely increased over the past years to the extent that a single plant with secondary metabolites are extracted and were evaluated for a new application area which may add to those known from the traditional medicines 44 .

Recently, bisbenzylisoquinoline alkaloids were found to have wide biological activities including antioxidant, cardiovascular effects such as antihypertensive and antiarrhythmic actions 45 , reversing the multidrug resistance (MDR) effect of human carcinomas, anti-HIV and as antituberculosis agents towards multidrug resistant 46,47 .

The curares are isolated from Chondodendron species (Menispermaceae) and curine is the most active constituent of the mixture which is known as arrow poison used by South American Indians. When injected into the bloodstream, it quickly blocks the neuromuscular action so that respiration ceases and death occurs. Actually, the curare alkaloids bind readily to the active site of the receptor for acetylcholine (Ach) at the neuromuscular junction, blocking the nerve impulse from being sent to the skeletal muscles which will effectively paralyze the muscles of the body 48 .

The tetrandrine, dauricine, daurisoline, and neferine are BBIQ alkaloids that have shown cardiovascular pharmacological effects and the mechanism of actions of these compounds was reviewed 49 . The antihypertensive effects of tetrandrine have been demonstrated in experimental hypertensive animals and patients because when calcium is added, it antagonizes the effect of tetrandrine in the interaction with the M-receptors, which means it can also be used as an antimalarial study based on in vitro culture of both chloroquine-sensitive and chloroquine-resistant strains of Plasmodium falciparum, this also showed that the antiarrhythmic effect of daurisoline is more potent than that of dauricine. Tetrandrine has a potential therapeutic value to prevent excess scarring in a patient with severe conjunctival inflammation. It has anti-inflammatory, immunologic, and antiallergenic effects. It inhibits the degranulation of mast cells. This action makes tetrandrine and related compounds are potentially useful in the treatment of lung silicosis, rheumatoid arthritis. 50 a. Antiparasitic activities of bisbenzylisoquinoline alkaloid:

A large number of antimalarial compounds with a wide variety of structures have been isolated from plants, and they can play an important role in the development of new antimalarial drugs using a template designed by ethnopharmacological researchers as this gave a promising pathway to plant metabolites and also to improve on the new properties 51 . Malaria is a mosquito-borne infectious disease caused by eukaryotic parasitic protozoans of the genus Plasmodium; typical symptoms include fever, fatigue, vomiting, and headaches. The disease is widespread throughout tropical and subtropical regions like Africa, Asia and South America, as it remains one of the major infectious diseases that threaten human lives. The quinine 4 and quinidine 68 were isolated from the bark of the cinchona tree which was the first effective antimalarial medicines and were used for centuries until chloroquine 69 was discovered in the 1940s. However, chloroquine is more effective for all types of malaria with fewer side effects until a resistant strain started to emerge in the 1960s, so other antimalarial agents with similar heterocyclic ring structures were developed afterward include mefloquinone 71 and mepacrine 72 52,53 (Figure 14).

Figure 14 : Malaria chemotherapeutics

A new type of antimalarial agent artemisinin was isolated in 1972 from the traditional Chinese medicinal plants, Artemisia annua (Asteraceae), which has been used for the treatment of fever and malaria since ancient times. Unlike most of the conventional antimalarial agents, artemisinin is a sesquiterpene lactone peroxide without a heterocyclic aromatic ring and was found to be more effective in treating malaria without obvious side effect. Because of its low solubility in both oil and water and its biotransformation, it is difficult to administer. Thus, many semisynthetic derivatives of artemisinin 73 have been synthesized including dihydroartemisinin 74, artesunate 75, artemether 76, and artemisone 77. Among these derivatives, artesunate 75 is commonly used in antimalarial combination therapy at the present time53 (Figure 15). 54 reported the in vitro activity of Triclisia patens and some BBIQ alkaloids against Leishmania donovani and Trypanosoma brucei which was aimed to improve the understanding and structural requirement for the antiprotozoal effects of plant secondary metabolites. In assessing the relationship on the antitrypanosomal structure-activity, it can be observed that the substituents present in each monomeric half of the molecule may influence antitrypanosomal activity. This means a comparison of the phenolic alkaloids tiliageine 79 with its methyl ether funiferine 78 suggests that the presence of a phenolic hydroxyl group results in a decrease inactivity. It can also be seen that most active bisbenzylisoquinoline alkaloids (IC50 < 10 µg/ml) for example, isotetrandrine 85, isotrilobine 2 and trilobine 1 had similar antiprotozoal activities against L. donovani and T. brucei. This could be explained by the fact that Leishmania and Trypanosoma species possess several molecular and biochemical features that are unique to the kinetoplastid order. In contrast, funiferine 78, and tiliageine 79 showed a selective activity towards L. donovani promastigotes, whilst the daphnoline 81, phaeanthine 84, berbamine 87 and trilobine 1 displayed selective activity against T. brucei (Figure 16).

So, Del Rayo Camacho et al

CHAPTER 1 : COCCULUS HIRSUTUS AND TRILOBINE / ISOTRILOBINE

A. Cocculus hirsutus 1. Ethnopharmacology

Herbal medicine is a major remedy in the traditional system that have been used in medical practices since antiquity and the practice continues to date because of its biomedical benefits as well as the place in cultural believes in many parts of the world which have greatly contributed to the maintaining of human health. Presently the world population depends on the plant-derived medicine for the first line of primary health care alleviation because it has no side effects 55 .

Most medicinal plants are important for pharmacological research and drug development, not only when plant constituents are used directly as therapeutic agents, but also as starting materials for the synthesis of drugs or as models for pharmacologically active compounds. The presence of radical scavenging potential in the plant helps us to understand that the plant possess bioactivity and some underlying molecule responsible for its activities. 56 Cocculus hirsutus (L.) Diels (common name ink berry) is an important medicinal plant belonging to the family Menispermaceae (the moon seed). They contain about 70 genera and more than 450 species, they grow mostly in a dry region of entire tropical Africa and Asia with their name distributed across the sphere 57 . This plant has an excellent ethnomedicinal potential to cure several ailments such as alleviating burning, sensation in eyes, taste, healing wounds in very less time. The roots, leaves, and fruits have immense medical properties to cure numerous diseases in an efficient manner.

Ethnomedicinal use of C. hirsutus plant:

The bioactive compounds produced during secondary metabolism are usually responsible for the biological properties of plant species used throughout the globe for various purposes, including the treatment of infectious diseases. Cocculus hirsutus is an important species of flowering plant belonging to family Menispermaceae. C. hirsutus is a widely used medicinal plant in famine food and pharmaceutical industries. Merchant et al 58 reported the ethnomedicinal value of C. hirsutus, which are used both for internal as well as external purposes. When the juice of the roots is mixed along with half spoon of sugar, it treats excessive body heat. While, the decoction of the roots, dried ginger, and long pepper is given along with milk to treat rheumatic pains, joint pains, diuretic, malaria, etc. Also, the roots are used for the antidote of snake bites. The juicy extract of the leaves is used to treat eye disease and soothing skin in eczema. However, if the extract of the mucilaginous leaves is taken along with water continuously for 2 -3 days, it cures dysentery, urinary problem, and tongue sore 59 .

B. Pharmacology

The C. hirsutus a useful agent of antimicrobial properties because it prevents infections, this may be a result of the phenolic compound presence and the tannins which acts as astringent that helps to heal wounds and ulcer 60 .

Anti-inflammatory and analgesic activities of C. hirsutus

The inflammation and pains are the most common health challenges treated with traditional remedies mainly the medicinal plant which provides relief symptoms with very little side effect when compare with chemical therapeutics. Sengottuuvelu et al. [START_REF]Synthesis of bisbenzyltetrahydroisoquinoline alkaloids containing one diphenyl ether link[END_REF] reported the analgesic and antiinflammatory activity of C. hirsutus in both in vitro and in vivo analysis. They used the human red blood cell (HRBC) membrane stabilization method for the in vitro anti-inflammatory activity and the induced granuloma in mice. From the phytochemical test, it showed the presence of phenolic compounds in methanolic extract of C. hirsutus which might suppress the formation of prostaglandin and bradykinins. More so if the noxious stimuli are depressed, it will reduce the number of writhes (peripheral effects) in animals and these writhing induced are due to the sensitization of nociceptors by prostaglandins.

The treatment with methanolic extract of C. hirsutus leaves at doses of 100 and 200 mg/kg reduced the number of writhes (45.51% protection and 64.5% protection) respectively. And among the two doses, 200 mg/kg showed a slightly lower analgesic activity than the standard drug indomethacin (70.5% protection) which showed the onset writhing was delayed and the duration of writhing was shortened. The same extracts showed significant anti-inflammatory activity in a concentration-dependent manner. The methanolic extract at 200 mg/ml concentration showed 53.7% protection of HRBC in hypotonic solution when compared with the standard Diclofenac which showed 65.2% protection.

After 6 days, the mean dry weight of granulomatous tissue surrounding the threads was significantly lower for the group treated with C. hirsutus extract as compared to the control group. The results indicate that C. hirsutus at the dose level of 100 mg/kg and 200 mg/kg produced a significant decrease weight of 57.4% inhibition and 68.3% inhibition as compared to the standard drug Naproxen 76.0% inhibition. From their results, they conclude that the methanolic extract of C. hirsutus possesses analgesic and anti-inflammatory activity.

Antibacterial activity:

The emergence of antibacterial resistance among bacteria has induced a compulsory need to search and identify newer novel, and safer antibacterial agents. 62 Over the past decades, intensive efforts have been made to discover clinical importance of antibacterial drugs of which 50% of all modern drugs are of natural product origin 63 .

Kalirajan et al. 64 tried to analyze the antibacterial activity of the leaves of C. hirsutus using methanolic and aqueous extract fractions for the in-vivo and in-vitro study against five pathogenic bacteria (Pseudomonas fluorecence, Escherichia coli, Klebsilla pneumonia, Sphylococcus aureus, and Vibrio Cholerae). According to their report, methanolic and aqueous extracts of C. hirsutus are the same level of activity than pure Streptomycin, they use as control.

The chloroform fraction extracted from the root of C. hirsutus showed maximum inhibition zone against pathogenic bacteria Pseudomonas aeruginosa and Bacillus cereus compare to Staphylococcus aureus, and no activity against Escherichia coli or Klebsilla pneumonia. The ethanolic or aqueous extracts also had no activity against all of these five stains. 65

Antidiabetic activity:

In 2007, Sangameswaran and Jayakar 66 reported the antidiabetic activity of the alcoholic leave extract of C.hirsutus on animals using rats in their study. The effect of C. hirsutus extracts showed a significant decrease in the blood glucose level to 165 mg/ 100 ml on long term treatment of 15 days, model at the doses of 400 and 800 mg/kg. Thus, the treatment restored the serum glucose levels almost nearer to normal values when compared with the glibenclamide as a standard. However, they also observed an increase in body weight of the animal (rats) after treatment unlike when the standard glibenclamide was used, probably the improvement in insulin secretion and glycemic control assisted to prevent loss of the body weight.

Antioxidant activity

In a step in this direction, we evaluated antioxidant potency of the ethanol extract on the aerial parts of Cocculus hirsutus Diels. The extract was investigated for its free radical scavenging action towards 1,1-Diphenyl-2-picryl hydrazyl (DPPH), nitric oxide (NO), superoxide and hydroxyl radicals and found that the ethanol extract shows promising free radical scavenging activity in dose-dependent manner. This antioxidant potency maybe related to the presence of antioxidant vitamins and phenolic compounds present in the extract. These results against free radical-mediated diseases clearly indicate that C. hirsutus Diel is effective C. Natural Substances in Cocculus hirsutus.

Das et al 67 reported that many scientists had carried out research on the chemical composition of C. hirsutus to contain essential oil, β-sitosterol, glycoside, tannins, and alkaloids. More so, Khushboo et al. 68 reported the preliminary phytochemical analysis of C. hirsutus leaves to have flavonoids, phenolics, saponins steroids, and tannins. The phytoconstituents exhibit a great deal of medicinal importance like terpenoids which act as antibacterial, anti-inflammatory, and antioxidant activity. The presence of phenolic components may act as antioxidant, and antimicrobial activity. Many flavonoids are shown to have antioxidant, free radical scavenging capacity, prevents coronary heart disease, anti-inflammatory, anticancer, and anti arthristis as this phytoconstituent can be used as a major tool for producing a quality control profile for a drug.

Another reviewer Bhavna and Bothara 69 reported the phytochemical constituents of C. hirsutus leaves to contain D-Trilobine 1, Isotrilobine 2, DL-Coclaurine 33 or 33b, and different sterols 89 (Figure 18), while Cohirsianine 90 70 , Jamtinine 91 71 Haiderine 92 72 , Cohirsine 93 73 , Shaheenine 94 74 , Hirsutine 95 75 and Cohirstinine 96 76 were extracted from stem and roots (Figure 19).

D. New interest of cancer therapy

Natural products in cancer therapy

Cancer is one of the leading causes of death in the world. According to the statistical data from the World Cancer Report, about 8.2 million people died because of cancer in 2012 which means 22,500 death per day, among them about 62% are from developing countries and 38% are from developed countries. So if this rate continues, 17.5 million cancer deaths per year can be predicted by 2050 77,78. In the 1950s, scientist began the search for anticancer agent from plant and the vinca alkaloids were discovered as the first natural products entering clinical use as an important class of anticancer agents. The vinca alkaloids are series of compounds composed of indole and indoline subunits isolated from Catharanthus roseus (L) (Apocynaceae family). After the success of the first clinical trials in the 1960s, clinicians started to use these called vinca alkaloids to treat different types of cancer, they were given the trade names of Velban®, Velbe®, and Oncovin®. Although some [START_REF]Spectral analysis of bisbenzyltetrahydroisoquinoline alkaloids[END_REF] Global cancer facts and figures. Am. Cancer Soc. Atlanta 2007. 78 Taxol (paclitaxel) 101, an active component extracted from the bark of Taxus brevifolia is used for years but overcame allergic reaction by using a lengthened infusion period and premedication. Nevertheless, taxol was approved by the FDA for the treatment of ovarian and breast cancer in 1992 and 1994 80 , because it was the first very active to treat these cancers. The semisynthetic analogue docetaxel 102 showed better activities in some assays than taxol 101 and was approved by FDA in 1995 81 .

Figure 21 : Anti-cancer drug from Taxol Another important anticancer natural product is podophyllotoxin 103 which was first isolated in 1880 from an alcoholic extract of Podophyllum (Podophyllaceae family) and it is a major component of podophyllin (Figure 22) which was found to inhibit mitotic spindle assembly. Although it side effect stopped it antitumor study. Fortunately, the synthesized podophyllotoxin glycoside as the etoposide 104 which entered into clinical use as an effective anticancer drug 82 . Later on, FDA approved etoposide drug for testicular cancer treatment. The first evocation of Trilobine or Isotrilobine date from the 1920s. At that time, Kondo and Nakazato have isolated alkaloids from Cocculus trilobus, and they named it trilobine 83 but with a wrong structure. Two years later, the same group isolated a second alkaloid from the same Cocculus trilobus, and called it homotrilobine because of its additional methyl 84 . The same compounds were then also isolated from Cocculus sarmentosus. 85 In 1932, Franz Faltis corrected the structure 86 to a dimeric molecule, but with a wrong position for the methoxy group in position 8' instead of position 5. Finally, after different publications, the definitive correct structure, with stereochemical configurations was established in 1962. [START_REF]Reaction catalyzed by palladium[END_REF]

Botanical and phytochemistry clarification

Usually, natural products are named with respect to the name of the plant from which they were isolated. So, naturally, when Kondo and Nakazato isolated alkaloids from Cocculus trilobus, they gave the name Trilobine. Then, they gave the name homotrilobine to the second alkaloids they isolated with an additional methyl. Unfortunately, in 1992, an American group had isolated a highly cytotoxic acetogenin from an Annonaceae Asimina triloba, [START_REF]Reaction catalyzed by copper[END_REF] and gave the name trilobin 105 (without a terminal "e") to this molecule, which has no relation at all to the trilobine 1 (with a terminal "e") from Menispermaceae Cocculus sp. (Figure 23).

Figure 23 Trilobine and Trilobin

Many plant species have "trilobus" as qualification that just means these plants are three-lobes leaves plants. It is not a problem for botanists, because plants are named with a genus name and with a species name, but it becomes problematic in chemistry when molecules are named with only one appellation. The opposite is also known, when a molecule is isolated separately from different plant and is named differently, and a single molecule finishes with two (or even more) different names. This is a real problem to which we have to pay close attention, and discussed in default to solve it.

First Synthesis of trilobine and related compounds

In 1962, Inubushi and Nomura were first to report the structure of trilobine and isotrilobine which were been isolated from C. trilobus DC. [START_REF]Reaction catalyzed by palladium[END_REF] Although, the stereochemical configuration of asymmetric centers and the secondary nitrogen were undermining the reductive fission with sodium in liquid ammonia by which the steric configuration of most bisbenzylisoquinoline alkaloids was decisively used even when this condition didn't give favourable result because of the presence of the dibenzo-p-dioxin system.

Then, some Japanese researcher Inubushi et al. 90 reported the first total synthesis of trilobine 1 and obaberine 41 alkaloids. They started by synthesizing the benzyl-8-bromo-N-norarmepavine 110 from the condensation of p-benzyloxyphenyl acetic acid 106 and 3-bromo-4,5dimethoxyphenethylamine 107 to form amide 108. The compound amide 108 was subjected to Bischler-Napieralski reaction to form the cyclized imine 109 and then reduced with NaBH4 to afford 110 (Scheme 4). The compound 117 was hydrolyzed by alkali to form a free acid which was converted into its p-nitrophenyl ester with p-nitrophenol in the presence of dicyclohexycarbodiimide (DCC) and removal of N-t-Boc with trifluoroacetic acid gave p-nitrophenyl ester phenethylammonium trifluoroacetate and was then cyclized to form the amide 120 in 36% yield (Scheme 8).

Scheme 8 : Hydrolysis/ amide reaction

With the Bischler-Napieralski reaction, the cyclization of the amide 120 affords the imine 121 and in such subjected to reduction with NaBH4 gave a diastereoisomeric mixture. Both diastereoisomers were separated by preparative TLC, and the expected compound 122, was obtained in only 17% yield. This diastereoisomeric compound (C1': -H) was isolated in 43 % yield. 122 was then treated with BBr3 in DCM at 0°C to form the per-O-demethylated compound 123. The next stage is the construction of the dibenzo-p-dioxin nucleus with a saturated solution of HBr heated in a sealed tube at 140°C for 3 hrs to afford the compound 124 and methylation with diazomethane provided 125, and on hydrogenolysis with platinum catalyst gave trilobine 1 (Scheme 9). The Isotrilobine is then directly obtained from trilobine by classical Eschweiler-Clark reaction 91 91 M. Tomita; Y. Inubushi, Pharm. Bull., 1955, 3(1), 7-11.

Scheme 9 : Synthesis of trilobine alkaloids

Although, their synthetic report showed that the structural feature of trilobine and isotrilobine alkaloids, both possess a dibenzo-p-dioxin nucleus, which is usually formed by Ullmann type of reactions. Their synthesis involved multiple steps, dramatically low yield, and unsymmetrically substituted derivatives. We aim to improve on the synthesis of this trilobine and isotrilobine alkaloids in our research study.

With respect to the very poor global yield of this synthesis (less than 0.00005 %), it cannot be possible to use it to be able to prepare trilobine and analogues, and we need to rethink all the strategy to improve this synthesis.

Our objective of this research comprised two distinct parts. The first is to develop a synthetic strategy that will be more efficient to provide the access to the dimeric structure in a shorter route. And secondly, to access the diverse bioactive compound of each molecule in other to validate its efficacy and medicinal used.

Since herbal plants have been consumed by men for their purported or documented health benefits; even during an era when their phytochemical constituent were not known. However, in recent times, scientific studies are beginning to confirm or refute quite a number of claims and assertions; by isolating, characterizing, identifying, and classifying the various secondary metabolites responsible for their actions. Also, factors that may be responsible for certain deleterious effects of herbal beverages are being identified. As of present, there is incontrovertible scientific evidence that herbal plants are a rich source of phytochemicals that possess a myriad of health benefits; including the prevention or management of central nervous systems (CNS) disorders such as mood, anxietyrelated, or neurodegenerative disorders.

The aim of this research work is to contribute to the existing body of knowledge about trilobine (C. hirsutus) as medicinal plants. Medicinal plants are used to cure diseases and several modern research have shown that the therapy of using plants is better than chemicals, by being safer besides having the synergistic effect of their active ingredient and presence of certain minerals.

Although, there is still a large number of medicinal plants in which the active constituents have not yet been investigated even though their effect is established by folklore and traditional system of medicine. So many reviewes on the medicinal values of C. hirsutus showed that it possesses various therapeutic properties. Taking into consideration the reported pharmacological actions and the traditional claims of C. hirsutus, we planned to achieve the below objectives.

We are looking for other syntheses of BBIQ derivative and to study them, to find a better solution for the synthesis of trilobine than the one presented, in 1977, by Inubushi and his group.

CHAPTER 2 : CHEMISTRY AND SYNTHESIS OF BBIQS ALKALOIDS

As just describe in the precedent chapter, the total synthesis of trilobine described up to now is not satisfactory. Some steps were very problematic, and the development of a new and efficient synthesis presupposes certain changes in protocol or pathway of these problematic steps. We are to focus then, by looking at all synthesis described for other BBIQ, and not from the type XXIII subgroup.

A. Elucidation of dimers:

The bisbenzyltetrahydroisoquinoline alkaloids comprise of over thirty subtypes which possesses structures containing two benzyltetrahydroisoquinoline nuclei joined by one, two, or three ether linkages. They occur in plants of Menispermaceae and other related families which include genera Berberis, Magnolia, and various species. The position and the number of ether linkages in the molecule have served as convenient criteria for the classification of these alkaloids into subgroups.

Diaryl ether cleavage:

In 1937, Sartoretto and Sowa 92 reported on the cleavage of diaryl ethers with sodium in liquid ammonia which was used before the advent of chromatography as a standard laboratory tool for the reaction. The simple mechanism for the reductive cleavage of diaryl ether is that the metal in liquid ammonia system is a rich source of electrons because the successive steps involves the addition of an electron to form an anion radical followed by protonation with the ammonia which acts as the proton source (Scheme 10).

Scheme 10 : Cleavage of diaryl ether by Sartoretto and Sowa

Cleavage of bisbenzyltetrahydroisoquinoline was believed to be a rich source of electrons to the system which forms an ion radical that is protonated by the ammonia prior to the cleavage. It was not until 1951 when Tomita et al. 93 applied this reaction to the isotrilobine 2 in the determination of the steric configuration of two asymmetric centres in bisbenzyltetrahydroisoquinoline alkaloids.

Since that time, this reaction has been utilized on different bisbenzyltetrahydroisoquinoline alkaloids and remain a useful method to elucidate the structure of dimers (Scheme 11). Scheme 11 : Cleavage of bisbenzyltetrahydroisoquinoline alkaloid by Tomita et al

Potassium Permanganate Oxidation:

The oxidation with potassium permanganate in aqueous acidic solutions has been used to determine the nature and substitution of the "south" part (bisbenzyl part) of various bisbenzyltetrahydroisoquinoline alkaloids. Shamma et al. 94 reported that oxyacanthine 82 could be converted to baluchistanamine 134 by treating with potassium permanganate in acetone and it is necessary to alkylate phenolic groups of the molecule prior to oxidation so that the dimeric nature of the product is not lost during phenolic oxidation. However, similar structural bisbenzyltetrahydroisoquinoline alkaloids underwent oxidation in an analogue manner which was formed by cleavage at the benzylic bond of isoquinoline moiety unsubstituted at C-8' or C-8 95 (Scheme 12). 94 In 1971 Bick et al. 96 reported the use of photo-oxidative cleavage reaction in some benzyltetrahydroisoquinoline based alkaloids that are useful in the structural studies of bisbenzyltetrahydroisoquinoline alkaloids. The laudanosine 135 unter ultraviolet irradiation in the presence of oxygen, gave mostly 3,4-dimethoxybenzaldehyde 136 in 67%, followed by 3,4dimethoxyterahydroisoquinolone 137 in 45% and the carbinoamine, which was reduced with sodium borohydride to afford 3,4-dimethoxytetrahydroisoquinoline 138 (Scheme 13).

Scheme 13: Photo-oxidative cleavage reaction of benzyltetrahydroisoquinoline alkaloids by Bick et al

According to them, this method has had only limited success when applied to the more complicated bisbenzyltetrahydroisoquinoline alkaloids. So they tried an example of isotetrandrine 85 alkaloids in the presence of oxygen which gave the dialdehyde 139 in 50% as the major product and amide-carbinolamine intermediate 140, isolated after reduction and its ready dehydration gave aminolactam 141 in 30%. However, this reaction was of great importance in the determination of the dibenzo-p-dioxine structure of bisbenzyltetrahydroisoquinoline alkaloids. So, the emergence of 96 I. R. C. Bick, J. B. Bremner, P. Wiriyachitra. Tetrahedron Lett. 1971, 12 (50), 4795 -4797.

oxidative photolysis is still an alternate to Na/NH3 cleavage in the elucidation of the structure of bisbenzyltetrahydroisoquinoline alkaloids by conversion of simpler products (Scheme 14). Scheme 14 : Photolysis of bisbenzyltetrahydroisoquinoline alkaloids by Bick et al.

With Reissert condensation:

In 1905 97 Arnold Reissert discovered that quinolone or isoquinoline could react with benzoyl chloride in the presence of aqueous potassium cyanide to form the resulting 1,2-dihydro-1-benzoyl-2-cyanoquinoline which was later named Reissert compounds and it became a versatile tool for different synthetic applications. However, Blasko et al. [START_REF]Gallic acid approach[END_REF] Scheme 15 : BBIQ synthesis by Reissert condensation.

Ullmann-type reaction:

The classical Ullmann-type synthesis of a bisbenzyltetrahydroisoquinoline alkaloids involves the direct coupling of a phenolic benzyltetrahydroisoquinoline with a halogenated in the presence of copper or one of its salts or oxides. The advantage of this approach is that the two halves of the dimers may be prepared, separated as pure enantiomers before the final coupling step although the yield is very low. The condensation of an equimolar amount of (S)-5'-bromolaudanosine 146 with (S)armepavine 35a in pyridine in the presence of pentafluorophenyl copper gave Otrimethylmagnolamine 147 in 42% yield (Scheme 16).

Scheme 16 : Ullmann type of reaction B. Synthesis of bisbenzyltetrahydroisoquinoline alkaloids containing one diphenyl ether link:

The simplest bisbenzyltetrahydroisoquinoline alkaloids are bases of mono ether typedauricine (type I) and liensinine (type V) were aimed at the first synthetic attempts. There exist two fundamental synthetic routes to such alkaloids from substituted diphenyl ethers by the addition of appropriate isoquinoline fragments using the known classical method (Pictet-Spengler or Bischler-Napieralski reactions) and linking substituted benzylisoquinoline bases by means of ether bridges.

Liensinines (Type V)

In 1966 Kametani et al reported the total synthesis of diastereoisomeric mixture of liensinine which was extensive degradative work on the liensinine alkaloids (Type V) with the composition of C37H42O6N2 that was isolated from the seed of Nelumbo nucifera Gaertn (also called sacred lotus) 102 . The result showed that the structure had a mixture of diastereoisomer that could not be readily separated. The reaction was carried out with O-methyl-Coclaurine 158, prepared by Bischler-Napieralski reaction, and then condensed by Ullmann type reaction with 3'-bromo-armepavine 149 to afford the diastereoisomeric mixture of methylliensinine 159which is not separable 103 (Scheme 20)

C. Synthesis of bisbenzylisoquinoline alkaloids containing two diphenyl ether link:

This group of alkaloids that are formed in plants by enzymatic double dehydrogenation of two molecules of coclaurine and its derivatives differing in the location of the ether.

Cepharanthine alkaloids (Type VI):

Cepharanthine is a member of bisbenzylisoquinoline alkaloids that possess a unique structural feature with the methylenedioxy group on its tetrahydroisoquinoline nucleus. Cepharanthine 160 is an ether-soluble, optically active, non-phenolic, amorphous tertiary base which can only be crystallized from benzene as the benzene adduct. It was earlier believed that this alkaloid (C37H38O6N2) contained three methoxyl groups and three oxygen bridges in the molecule, but careful functional group determination 104 showed that there are two methoxy groups, two ether bridges, and one methylenedioxy group (Figure 25). On the other hand, the hydroxy function of the nitrostyrene 169 was protected with ethylchloroformate and then reduce under Clemmensen condition to yield to phenethyl derivative. Then, the amino group was protected as carbobenzoxy derivative and phenol was selectively deprotected by aqueous sodium bicarbonate to form 170. Its condensed with 165 was performed by Ullmann reaction with cupric oxide as catalyst and the formyl group was remove by hydrochloric acid in methaznol, yielded to 171 in 27% yield for the two last step (Scheme 23).

Scheme 23 : Synthesis of diphenyl ether by Tomita et al

With the northern part of their synthesis in hand, they tried to synthesize the south part that formed the intermediate of cyclobisamide 176 through the diphenyl ether diacetic acid mono-ester 174. This was prepared with p-hydroxyphenyl acetic acid 172 which was first brominated and then methylated to form 116. The same acid 172 was converted to the tert-butyl ester 173. The use of different protecting groups on the acid was to have a selective removal of the protective group through the acidic medium. Thereafter, Ullmann condensation was performed to form, after hydrolysis of the tertbutyl group, the corresponding 174 but only in with 13% yield for these two steps (Scheme 24). Bischler-Napieralski reaction of the cyclobisamide 176 with POCl3 in chloroform gave a mixture of 3,4-dihydroisoquinoline 177 with two spots on the thin layer chromatography. This mixture was hydrogenated over a platinum catalysts to avoid the ready oxidation with atmospheric oxygen and the product was reduced with sodium borohydride to afford a mixture of Nmethyltetrahydroisoquinolines 160. These products were separated and purified with a preparative TLC and column chromatography on alumina, the Rf value and NMR data of the synthesized molecule was identical with natural cepharanthine 160 (Scheme 26).

Scheme 26 : Synthesis of Cepharanthine by Tomita et al However, the cepharanthine drug is mostly used in Japan since the 1950s to treat acute and chronic diseases like leukopenia, snake bite and others. It is approved for human use in the large class of bisbenzyltetrahydroisoquinoline alkaloids, as it is mainly isolated from the plant Stephanta cephalantha Hayata. They tend to exhibit multiple pharmacological properties like antioxidant, antiinflammatory, anticancer, antiviral, and antiparasitic properties 108 .

Tetrandrine (Type VIII)

Tomita et al 106 , 109 made an attempt to accomplish the total synthesis of alkaloids possessing two asymmetric centers in the oxyacanthine 82berbamine 87 series of bisbenzyltetrahydroisoquinoline alkaloids, but unforeseen complication prevented the completion of the synthesis except for the cepharanthine (racemic). But in 1968, Inubushi et al 110

Scheme 27 : Synthesis of tetrahydroisoquinoline alkaloids by Inubushi et al

The tetrahydroisoquinoline 182 derivatives were resolved into its optical antipodes by means of (+)-tartaric acid. The Ullmann condensation of (S)-8-bromo-laudanidine 183 with N-tbutoxycarbonyl phenylethylamine 170 afforded a diphenyl ether 184 in 50% yield. After hydrolysis, followed by Ullmann condensation with methyl p-bromophenylacetate 185 gave N-tbutoxycarbonyl-carboxylic acid methyl ester 186 in 40% yield, the protecting group in the diphenyl ether was removed and then condensed to form cycloamide 187 in 54% yield (Scheme 28).

Scheme 28 : Ullmann-type/ Hydrolysis reaction by Inubushi et al.

The Bischler-Napieralski reaction of the cycloamide 187 afforded the dihydroisoquinoline 188 derivatives. The direction of this reaction can be ultimately settled by the identification of the final product of isotetrandrine and pheanthine. The reduction of the dihydroisoquinoline 188 with NaBH4 followed by N-methylation gave a mixture of isotetrandrine 85 in 60% and pheanthine 84 in 40%. Thus, the ratio of these two alkaloids in the mixture was estimated by comparing the NMR spectrum of the two mixed with the natural products respectively. Although, the separation of each diastereoisomer from the 188 was effected by the aid of different solubilities of 85 and 84 picrates as the less soluble 84 picrate tends to crystallize and the solution removed from crystallization contained mainly isotetrandrine 85 (Scheme 29).

D. Synthesis of some bisbenzyltetrahydroisoquinoline alkaloids containing three diphenyl ether link:

This group of bisbenzylisoquinoline alkaloids possesses a structure that includes three oxygen bridges consisting of one diphenyl ether linkage and a diphenylene dioxide ring. These alkaloids may be considered as the off-springs of coclaurine and of the oxyacanthine-berbamine series. WE had already presented, in chapter 1, the total synthesis of Trilobine and Isotrilobine with all its flaws, and to improved it, we have to look carefully the synthesis described for others BBIQ type XXIII with the three ethereal ether bridge linkage.

Methyldihydromenisarine (Type XXIV)

In the preparation of a bisbenzyltetrahydroisoquinoline alkaloids with three diphenyl ether linkages, an Ullmann reaction was used to build the important symmetrical diamine which incorporates the diphenylene dioxide bridge. Menisarine is an alkaloid isolated from Cocculus

Tiliacorine (Type XVIII)

In 1971, Anjaneyulu et al 113 reported the synthesis of diastereoisomer tiliacorinine alkaloids which were isolated from Tiliacora racemose Colebr, and the structure has been assigned on the basis of extensive degradation. The unique feature of this alkaloids is that it has two diaryl ether linkages and one aryl-to-aryl linkage which are mostly member of bisbenzyltetrahydroisoquinoline alkaloids.

An Ullmann reaction between the 5-bromovanillate 202 and methyl-3-bromo-4hydroxybenzoate 203 to yield all the three possible dicarbomethoxy-dibenzo-p-dioxins 204 in 40% yield, which was separated by chromatography 114 . The diester 204 was reduced with LAH in THF to form diol 205 which was then converted with thionyl chloride to afford the dichloride 206 and was treated with KCN to form dinitrile 207 in 13% yield which was equally reduced in the presence of Raney nickel to afford the bisphenylethylamine 208 in 9% yield (Scheme 33).

Scheme 33 : Synthesis of dibenzo-p-dioxin by Anjaneyulu et al.

The same trouble of non-selective double Ullmann cross-coupling observed by Anjaneyulu et al. for the preparation of dibenzo-dioxin 204, was also observed by Pachaly and Schäfer, when they try to form dioxin using two different bromo-hydroxy phenethylamines (Scheme 34) for their synthesis of tiliacorine. 115 One more time, for the synthesis of tiliacorine, these authors, Anjaneyulu, and Pachaly as well, have preferred Bishler-Napieralski reaction compare to Pictet-Spengler, even if the yield obtained is very low and non-selective.

E. Spectral analysis of bisbenzyltetrahydroisoquinoline alkaloids

The elucidation of the structure of benzyltetrahydroisoquinoline alkaloids may be facilitated with the assistance of the following spectra analysis like the mass spectroscopy, nuclear magnetic resonance, X-ray analysis, UV spectroscopy.

In mass spectroscopy, the main cleavage that the Coclaurine alkaloid undergoes is at C-1 to C-α bond, which is doubly benzylic and also adjacent to the nitrogen atom. The base peak and intense peaks differ due to the loss of the methyl group from the base ion. Since the charge prefers to remain on the nitrogen atom, the ion representing the C ring of the molecule shows up only as a minor peak 116 (Scheme 37).

Scheme 37 : Mass spectroscopy of coclaurine alkaloid

Although, David G.I. Kingston reported the X-ray study of alkaloids isolated from Anisocycla grandidieri (Menispermaceae), and which was revealed by the crystal analysis to be the cocsuline 218. The C-6 methoxyl points away from the C-7 hydroxyl and the C-1 hydrogen is beta. The ring C plane is nearly perpendicular to the mean plane of tetrahydroisoquinoline ring system. Since the nitrogen is secondary, ring C lies on the nitrogen side 117 (Figure 28). The NMR spectroscopy has been firmly established that in most Nmethylbenzyltetrahydroisoquinolines, rings A and C lie on the same side of the molecules, while the ring C is preferentially located on the same side as the tertiary or quaternary nitrogen. For example, the laudanosine in CDCl3, H-5 is at δ7.68 and H-8 is at δ 7.49. The C-6 and C-7 methoxys are found at δ 4.12 and δ 4.00 respectively. In most cases, minor differences in chemical shifts appear if an additional methoxyl is present at C-8. While the 13 C chemical shift has been assigned and will be noted that the C-6' signal comes at 121.5 ppm since this carbon atom is deshielded with respect to its ortho and meta methane neighbors by its lacking the strongly shielding influence of ortho and methoxy groups118,119 (Figure 30). To successfully separate the alkaloids mixtures of bisbenzyltetrahydroisoquinoline, a preparative process was carried out in multiple extractions by using solvents of different polarities and pH values. As the final purification is achieved by chromatography ranging from thin-layer chromatography, which in most cases is replaced by automated high-pressure chromatography and the still standard for cleaning is column chromatography 121 . Thus, on elucidating the structure of the compounds, analytical spectroscopy has been used such as UV, IR, NMR, and MS that serve as a standard in past decades and as well replace the classical method of degradation and derivatization. Although a complete 1 H and 13 C NMR data sets for many bisbenzyltetrahydroisoquinoline alkaloids characterized in the past century are still lacking. In many cases, the interpretation of 1 H NMR data of unknown molecules relied on the standard work of Guinaudeau and Shamma, on the spectral characteristics of tail-to-tail connected bisbenzylisoquinoline, or on comparisons with related substances. Only in very few cases, absolute configurations were determined by X-ray crystallographic analysis 122 .

For analytical purposes and in particular, for the separation, determination, and quantification of alkaloids in plant extracts for medical applications, HPLC methods proved to be a powerful tool. The bisbenzyltetrahydroisoquinoline mixtures with up to 15 compounds can be separated after the evaluation of various solid-phase materials and optimization with acidic or basic buffer systems 123 . Moyer 2004, detailed more look into the complex alkaloid compositions that allow the combinations of LC with MS analyses which established a library with the MS fragmentation pattern of 20 alkaloids belonging to 10 different bisbenzyltetrahydroisoquinoline types124 .

CHAPTER 3 : RESULTS AND DISCUSSIONS

As already presented in the preamble, the project of total synthesis started some years ago, in Toulouse. The first biological results of trilobine and isotrilobine as epigenetic modulators were very encouraging, but unfortunately the structures we were able to prepare in a derivatization program from natural trilobine never improve enough of these results. Error! Bookmark not defined. The only opportunity we had to find a "drug candidate" was to develop a synthesis, efficient and flexible enough to be able to have access to unnatural BBIQ type XXIII, and thus to have access and propose new biologically active molecules against cancer.

A. Retrosynthesis

The retrosynthesis analysis of trilobine and isotrilobine alkaloids could be accessed from connection of four synthon, two arylacetic acid derivatives and two phenethylamine. The two isoquinoline moieties should be prepared using Pictet-Spengler or Bischler-Napieralski reaction (Error! Reference source not found.. The final step should be this macrocyclisation reaction by the formation of the dioxiran system. This dioxiran system have to be install by reaction of a catechol derivative and a dihalogeno substrate, at the end of the synthesis to avoid problem in the regioselectivity of this double reaction as already discussed in chapter 2. Scheme 39 : synthesis of fluorinated derivatives Several attempts were engaged to perform the nucleophilic substitution of the fluorine atom by a hydroxyl functional group using classic procedure i.e. by heating the two reagents in DMF with base. Several bases were used, carbonate or hydroxide, but no simple coupling product was obtained. The crude reaction mixture we were able to recuperate showed a large variety of compounds, from degradation product to oligomers and we had to conclude that the high reactivity of benzylic position in such substrate as compound 221 prohibited this pathway.

Scheme 40 : Aromatic nucleophilic substitution reaction

We then turned our attention to metallocatalyzed coupling reactions.

Metal catalyzed diarylether coupling reaction.

As an alternative to the nucleophilic aromatic substitution, we turn our effort to the metal catalyzed coupling reaction for the preparation of our diaryl ether moiety. Borylated derivatives are often used for such reaction, and former works from the lab were already done. Scheme 41 : Retrosynthetic scheme for diaryl ether synthesis from arylboronic acid. a. Reaction catalyzed by palladium.

The coupling reaction mediated by palladium is a Suzuki type reaction but using a phenolate as ligand, which is able to react with the arylic moiety during the reductive elimination step to form a diaryl ether. The preparation of the borylated partner was already described by Ghosh et al. in a 5 steps sequence from commercially available 4-hydroxyphenylacetic acid with a 75% overall yield. 126 Later, two other groups used the Ghosh's sequence to prepare the same compound 226 but with lower efficiency for the borylated step. Scott in 2011 obtained less than 80% 127 and Yokokawa in 2016 only 60%. 128 Scheme 42 : Synthesis of aryl boronic acid by Ghosh et al.

Precedent students in the group tried to reproduce the same sequence using the same protocols described. Unfortunately, in their hands, the result for the borylation reaction was much closer to the one of Yokokawa than the one of Ghosh. Nevertheless, the borylated compounds 226 were engaged 126 Ghosh, S., Kumar, A.S., Mehta, G.N.; Soundararajan, R.; Synthesis, 2009, (19), 3322-3326. 127 Scott, J.M., Baccei, C., Bain, G.; Broadhead, A., Evans, J. F., Fagan, P., Hutchinson, J. H., King, C., Lorrain, D. S., Lee, C., Prasit, P., Prodanovich, P., Santini, A., Stearns, B. A., Bioorg. Med. Chem. Lett. 2011, 21(21), 6608-6612. 128 Yokokawa, F., Nilar, S., Noble, C. G., Pheng Lim, S., Rao, R., Tania, S., Wang, G., Lee, G., Hunziker, J., Karuna, R., Manjunatha, U., Shi, P-Y., Smith; P.W., J. Med. Chem. 2016, 59 (8), 3935-3952. in a coupling reaction with the hydroxyphenylacetate 227. Different conditions were used using a different sources of palladium, ligands, but yields in compound 228 were never up to 55-60%. Scheme 43 : Scheme for coupling of arylboronic acid with palladium.

At that time, these yields for borylation reactions and coupling reactions were judged too low to propose an efficient preparation of substrates, so an alternatives with higher yield were looked after.

b. Reaction catalyzed by copper.

Instead of palladium, copper salt was also described to perform this coupling between phenol and a borylated aryl derivatives. Most of the examples reported in the literature described intramolecular coupling, but some examples also shows intermolecular cross-coupling.

For example, Kozikowski and co-workers described the coupling between arylboronic acid and eugenol 229 (or β-γ dihydroeugenol) mediated by cuprous acetate with variable efficiency depending of the substituent on aromatic boronic acid 129 . Another example was presented by Georghiou and coll. for the elaboration of the synthesis of (-)-tejedine, a minor seco-bisbentetrahydroisoquinoline alkaloid (a type VI BBIQ) in Berberis vulgaris (berberidaceae), they used cupric acetate with pyridine for the coupling of an arylboric acid with a disubstituted phenol with a 57% yield130 .

Scheme 45 : Copper catalyzed coupling reaction of arylboronic acid

With regards to the yield obtained by all of those groups, not better and even worse than those we have obtained with palladium, investigations with arylboronic acid were discarded and even more with the comments Evans did after his study of coupling arylboranic with activated phenol 131 . Evans underlines the fact that if ortho-substituted phenol reacts with good yield, it is the opposite of orthosubstituted boronic acids. In our case, this is an undesired limitation. Finally, without better results than those given by Ullman-type reaction, we decided to study and improve the known and described conditions.

C. "North part" of Trilobine

Dibenzo-p-dioxiran system

As we had already presented in precedent chapters, several sequences for the formation of dibenzodioxiran system. Two main ways lead to this system; double Ullmann-type coupling reaction or double aromatic nucleophilic substitution reaction.

Figure 32 : Strategies for dibenzodioxiran system synthesis None of the two Ullmann-type coupling reaction pathways were satisfactory, as regards to all procedures described in the literature and presented in chapters 1 and 2. Yields of the coupling are often low and selectivities are not good at all. So, in our part we looked for the third pathway, with double aromatic nucleophilic substitution. As preparative work, we checked if this reaction was effective or not. Thus, we engaged protected N-Boc dopamine 235 and protected N-Boc dichlorophenylethylamine 234 in typical conditions and obtained the desired dibenzo-p-dioxiran system in 68% yield as shown in table 5. Because of symmetry, we can not be sure we did not obtain mixture of "cis" and "trans" regioisomer, while NMR spectra are not distinguishable, and of course even less mass spectra. Only X-ray analysis would give clues, but only in a lonely crystal. We did not optimized this reaction. At this stage, our goal was to check the feasibility. In our strategy, this reaction for the formation of the dibenzo-p-dioxiran system is planned to be the last critical step, after the formation by cyclization of the two tetrahydroisoquinoline. So, we hope that an intramolecular process will limit the regioselectivity problem.

Scheme 46 : Synthesis of dibenzo-p-dioxiran system

We need now to develop the preparation of the two phenethylamine, and study the tetrahydroisoquinoline cyclization process.

Dihalogeno-phenethylamine:

One of the classical pathways to access to the benzyltetrahydroisoquinoline alkaloids is the Bischler-Napieralski132 intramolecular condensation or Pictet-Spengler reaction. 133 As shown in precedent chapters, the majority of authors prefer the Bischler-Napieralski cyclization as one of the choice methods used for the preparation of simple benzyltetrahydroisoquinolines, through an amide formation obtained from the substituted phenethylamine and acid.

We advance our synthesis to form a monomer of benzyltetrahydroisoquinoline derivative. So, we activated the compound 224 with oxalyl chloride in a catalytic amount of DMF in DCM for 2 hrs into acid chloride and without purification, the commercial available dichlorophenethylamine 237 was added to the mixture to stirred at 0°C for 4 hrs134 , to form the key amide 238 intermediate in 65% yield (Scheme 47).

It is obvious that the Bischler-Napieralski intramolecular condensation can be performed with this intermediate of the amide to afford the benzyltetrahydroisoquinoline alkaloids.

Scheme 47 : Synthesis of amide 238

Having the amide 238 in hand, we condensed it intramolecularly with POCl3, in acetonitrile under stirring at 90°C for 4 hrs which is known as Bischler-Napieralski reaction to form the iminium salt. On reduction with NaBH4, at 0°C for 4 hrs, the compound could not be isolated properly. But we observed a traces of two singlet which differs from the three spin system of the amide. Because it was carried out on low scale medium, it was difficult to purify the compound 239 after several attempts (Scheme 48). Scheme 48 : Bischler-Napieralski reaction of 238 Unfortunately, it was not possible to investigate this transformation more ahead because of restriction policies of the chemical company which supplies the starting dichlorophenethylamine 237 at an affordable price. We sought alternative routes to overcome our problem and that was to develop the different monomer of phenylethylamine derivatives with diiodo substrates, it will give a better formation of dibenzo-p-dioxine nucleus. Iodide is a preferred choice of Ullmann type coupling because it's stable and more reactive under Ullmann conditions than the other halide compounds.

Based on these challenges and difficulties encountered to obtain the iodinated compound 241 directly from 240 we proceed further to performed iodination reaction on nonbenzylic substrate. 240 was thus, reduce into alcohol 244, via reaction of DiBAl-H on correspondiong methylester 243. (Scheme 51).

Scheme 51 : Reduction of Ester 243

With 4-iodophenyl alcohol compound 244 in hands, the Mitsunobu reaction with phthalimide 245 as nucleophile was attempted. The reaction worked very well and we isolated the compound 246 in 91% yield. We checked first the effectiveness of hydrazine to liberate the free amine. 136 On the completion reaction mixture, 4-iodophenylethylamine 247 was obtained in 91% yield (Scheme 52). After checking that, we were able to regenerate iodophenethylamine derivative 247 from phthalimide 246, we looked after conditions for the monoiodination of 246.

So, we engaged it into oxidative iodination using the Kraszkiewicz et al., method which we have used in our previous experimental procedure to form compound 241. Unfortunately, the reaction was not successful rather the product got destroyed after the workup. Probably, the condition was too harsh (Scheme 53).

Scheme 53 : Iodination of compound 246

Since achieving the diiodophenylethylamine with the above sequence may not be possible. We have to resolve to another approach and this time around, we used the commercially available 4iodobenzoic acid 249. Even though it involves multiple steps to get the diiodo compound but it will form a better yield on Ullmann reaction of dibenzo-p-dioxin nucleus.

So we engaged compound 249 according to Kraszkiewicz et al. 135 method which has been used in our previous experimental procedure. The reaction worked very well and it was recrystallized with CCl4 to form 3,4-diiodobenzoic acid 250 in 91% yield without any over iodination and subsequently, we subjected 250 with thionyl chloride in MeOH to form methyl-3,4-diiodobenzoate 251 in 95% yield (Scheme 54).

Scheme 54 : Double iodinated ester 251

This diiodobenzoate 251 was reduced with Dibal-H and gave the diiodo alcohol 252 in 86% yield. This alcohol 252 was oxidized with IBX according to Nadkarni et al. 137 and we obtained the diiodobenzaldehyde 253 in 54% yield (Scheme 55).

Scheme 55 : Double iodinated aldehyde 253

We also tried to transform the 3,4-diiodobenzaldehyde 253 using Henry's reaction which is a well-known classic reaction with MeNO2 in the presence of NH4OAc in acetic acid. The reaction gave the corresponding 3,4-diiodobenzene nitrostyrene 254 in 79% yield and the 3,4-diiodobenzene nitrostyrene 254 was then reduced with LAH in THF. Unfortunately, we could not obtain the 3,4diiodophenylethylamine 255 product in a purified form (Scheme 56).

Scheme 56 : Diiodinated phenethylamine 255

With these disappointing results from 3,4-diiodophenylethylamine 255 approaches, we were motivated to find another procedure to circumvent the use of diiodophenethylamine. Thus, it was envisioned that 3,4-dichlorobenzaldehyde 256 could be an attractive equivalent for the above diiodophenyethylamine compound and could be used to give rapid access to trilobine.

So, the dichlorobenzaldehyde 256 was engaged into Henry's type of reaction, interestingly we obtain the corresponding dichloronitrostyrene 257 in 83% yield. Although the similar challenge we encountered on diiodophenylethylamine 254 came up, the Petersen et al. method138 with Dibal-H in THF. We were able to resolve the problem through the conversion of the 3,4-dichloronitrostyrene 257 into 3,4-dichlorophenyethylamine 237 in 93% yield (Scheme 57).

Scheme 57 : Synthesis of dichlorophenylethylamine 237

The crude dichlorophenylethylamine 237 had some residues of Dibal-H and it was challenging to get it purified by column chromatography in a pure form. Due to high polarity and some instability of free amine 237 during purification process, we decided to protect it directly by Boc derivative. This reaction worked very well and it was easier to purify by column chromatography to form the corresponding N-Boc-3,4-dichlorophenylethylamine 234 in 84% yield (Scheme 58).

Scheme 58 : Protection of dichlorophenylethylamine 237

Concerning the problem of commercially affordable supply of dichlorophenethyl amine 237, with this sequence, we were now able to focus our attention on the intramolecular cyclization for tetrahydrosioquinoline alkaloids. This approach would provide general and straightforward access to preparing amine and aldehyde which is an entry to the natural product of trilobine 1 or isotrilobine 2. But, we also need to study cyclization process, inefficiency and in regioselectivity, with di-and trihydroxy phenethylamine derived from dopamine and gallic acid.

D. Tetrahydroisoquinoline synthesis a. Dopamine approach

To take the advantage of cyclization process in constructing benzyltetrahydroisoquinoline derivatives, we adapted our proposed retrosynthetic analysis using dopamine hydrochloride salt 7*HCl and any synthesized simple building block of the molecule, which could finally be cyclized with BF3-OEt2. After the construction, the obtained benzyltetrahydroisoquinoline compound could then be used to form the coupling of dibenzo-p-dioxin nucleus and diaryl ether through Ullmann type reaction. Hopefully, the trilobine or isotrilobine compound could be accomplished by this process.

Figure 33 : Retrosynthesis analysis of benzyltetrahydroisoquinoline alkaloid Dopamine is known for its therapeutic effect in the treatment of Parkinson's disease which makes it interesting in both biological and medicinal fields. More so the synthetic organic chemist have regarded it as a useful building block to design and synthesize some biological active compound because it possesses an amino group and catechol moiety 139 .

The dopamine hydrochloride 7*HCl was protected with boc according to M. Sani et al. 140 method. The reaction worked well and formed the desired product of dopamine with boc 235 in 94% yield. Then, it was reacted with dimethyl sulfate in the presence of K2CO3 as the base, and on the completion, it was isolated to form the compound 258 in 88% yield (Scheme 59). Now, we tried to engage 3,4-dimethoxyphenylethylamine protected with boc 258 with panisaldehyde 259 to form a cyclization in the presence of an acidic medium and this can be achieved by Pictet-Spengler type reaction. The Pictet-Spengler reaction is a well-known type of reaction that involves the condensation of tetrahydroisoquinoline skeleton mostly present in many molecules of physiological and therapeutic use141 .

We used the protected dopamine with boc 258 and commercial p-anisaldehyde 259 as a model for the cyclization process of the aromatic ring system with BF3-Et2. The reaction worked very well and it was isolated to form the compound 260 in 91% yield.

Subsequently, we tried the same condition on protected dopamine with boc 258 and our synthesized 3-iodo-4-methoxybenzaldehyde 261. The corresponding compound 262 was obtained in 90% yield (Scheme 60) Thus, with dopamine hydrochloride salt 7*HCl in hand, we treat it directly with diphenyldichloromethane according to Takaoka et al. 142 and it affords the corresponding 266 in 80% yield and this product can easily be deprotected by hydrolysis under acidic condition 143 . Moreover, we proceed further to protect the free amine of diphenyl dopamine 266 with boc as reported in our previous experimental procedure to form the compound 267 in 66% yield (Scheme 62). Scheme 62 : Protection of dopamine with boc Now, we tried to cyclize the protected diphenyl group on dopamine compound 267 and our synthesized 4-methoxyphenyl acetaldehyde 263 under the same condition of acid catalyzed condensation with BF3-Et2O. The expected formation of a benzyltetrahydroisoquinoline 268 was unsuccessful. Probably the deprotection of diphenyl groups occurs in the reaction mixture since it is acid sensitive and the by-product may have complexed with the desired product making it difficult to isolate them (Scheme 63).

Scheme 63 : Pictet-Spengler reaction of 267

Having the methodology for the preparation of tetrahydroisoquinoline in hands, we now turn our attention to trioxygenated substrate instead of the two oxygenated dopamine. Gallic acid is the starting material of choice, sinceits transformation to phenethylamine derivative can be performed using the Henryreaction/reduction sequence, we have already used with halogenated benzoic acids.

Scheme 68 : Pictet-Spengler reaction of 277

We understand that with diphenyl protection in both dopamine and gallate, it was always difficult to perform the intramolecular cyclization by Pictet-Spengler reaction because the diphenyl molecule is sensitive to acidic medium. However, we have to engage the diphenyl protection of gallate by Bischler-Napieralski reaction to acertain if the same challenge.

c. Iodophenylethylamine approach

Although the Tangdenpaisal et al. 134 method used earlier in our experiment gave us a good yield, it is moisture sensitive and so we sought for another method free from previous challenge. Since the acid chloride compound is moisture sensitivity. We found out that Cox et al 145 could actually work well on one spot condition to form the amide which will enable the intramolecular cyclization reaction. So we engaged 4-iodophenylethylamine 247 to react with 4-iodophenyl acetic acid 240 in the presence of HBTU and Et3N as a base in DMF at 0°C for 24 hours. The reaction worked very well and we isolated the amide compound 280 in 89% yield (Scheme 69).

Scheme 69 : Amide reaction from iodophenylethylamine

We tried a model with a free hydroxyl group, using the commercially available tyramine 29 and 4-hydroxyphenyl acetic acid 172 respectively to react under the same condition of Cox et al.

Unfortunately this reaction didn't work, probably the free hydroxyl group complexed with the HBTU reagent in the medium and it resulted to a mixtures of multiple compounds that was very difficult to isolate. To overcome this problem, we found out that the report of Valpuesta et al. 146 which they developed in their synthesis could actually solve our problem. So we tried their procedure with the tyramine 29 and the 4-hydroxyphenyl acetic acid 172 was heated and the reaction worked very well and we isolated the amide compound 281 in 89% yield (Scheme 70). Scheme 70 : Amide reaction from tyramine We subjected the amide compound 281 as a model to a Bischler-Napieralski intramolecular condensation reaction through POCl3 in acetonitrile, but TLC control of the reaction showed multiple spots. After reduction with NaBH4, it was very difficult to isolate anything from the mixture, maybe the free hydroxyl group could have affected the cyclization process by reacting with the POCl3 reagent in the medium (Scheme 71) Scheme 71 : Bischler-Napieralski reaction However, we engaged dopamine 7 and 4-hydroxyphenyl acetic acid 172 by the same method 146 , the reaction worked very well and we isolated the amide compound 283 in 90% yield (Scheme 72). We find out this approach is more efficient and convenient, because no reagent or an activating agent is required and no side product is formed. Since our previous attempt to cyclized free hydroxyl compound using Bischler-Napieralski reaction didn't work, so we never bothered to engage 283 into it. Rather, we coupled the amide compound 283 and a monomer of halide substrates of amide into diaryl ether formation using Ullmann type reaction.

Scheme 72 : Synthesis of amide coupling of dopamine

With these promising results, we extend Valpuesta et al. 146 method to phenethylamine compound 277 and 4-hydroxyphenyl acetic acid 172. These gave us the desired amide compound 284 in 62% yield without any side reaction (Scheme 73).

Scheme 73 : Synthesis of amide coupling

Therefore, the amide compound 284 was engaged into a Bischler-Napieralski reaction, which is usually mediated by POCl3 in acetonitrile. The reaction formed an intermediate imine compound and it was subsequently reduced with NaBH4. We obtained traces of compound 278 of benzyltetrahydroisoquinoline in a complex mixture that was very difficult to separate (Scheme 74).

Scheme 74 : Bischler-Napieralski reaction of 284

From the aforementioned reactions, Valpuesta et al 146 . method proved to be more efficient and convenient which proved to be compatible with amide reaction. Based on these premises, we decide to engage the 3,4-dichlorophenylethylamine 237 with different halide substrates of 4methoxyphenyl acetic acid (225 and 285) and it gave us the corresponding amide compound of 286 and 287 in 82% and 59% yield respectively (Scheme 75).

Scheme 75 : Synthesis of amide coupling

We have now in hands, the two phenethylamine; the catechol on the one hand, and the dichlorophenyl on another hand. We knew that it is possible to perform a reaction between them to form the dibenzo-dioxiran system, and we began to study the cyclization process via Bischler-Napieralski or Pictet-Spengler reaction. The last point to be solved is the formation of diaryl ether moiety corresponding to the "south part" of the trilobine, correctly functionalized i.e. with two masked aldehydes which will need to be regenerated separately.

E. Synthons for "south" part of trilobine

As the aromatic nucleophilic substitution can't be efficient, we turned our attention to other halogenated substrate and replaced the fluorine with iodine, bromine, or chlorine.

The first attempt is synthesizing the latter synthon with the commercially available 4hydroxyphenyl acetic acid 172. It reacted with dimethyl sulfate in the presence of potassium carbonate according to M. Sani et al. 140 to yield to bisprotected compound 223 in excellent yield.

When we tried to increase on a large scale, we had the challenge of isolating the product from column chromatography because of the sulphate residues in the compound and this made us use Kugelrohr distillation which is more efficient to remove the sulphate residues but may not be a convenience for large scale production. We sourced alternate methylation reagent and we tried dimethyl carbonate under the same method. This time around, it was easier to purify even on a large scale because the by-product is carbon dioxide and it is volatile, but the yield is slightly lower if compared with dimethyl sulphate (Scheme 76) Scheme 76 : Methylation of acid So the methyl-4-methoxyphenyl acetate 223 was allowed to undergo a reduction reaction 147 using diisobutylaluminium hydride (1 M, Dibal-H) in THF for 1 hr at -78°C, to afford the corresponding aldehyde 263 in 23% yield. The product was very difficult to isolate because it degrades on silica gel during column chromatography (Scheme 77). Scheme 77 : Dibal-H reduction of ester.

We engage methyl-4-methoxyphenyl acetate 223 in a mild reduction process with NaBH4 in THF to reflux at 65°C for overnight. Unfortunately, all the starting material was not consumed rather it gave alcohol of 42% yield 288 and 56% yield of starting material 223. Since this method isn't given complete conversion, we performed then, the reduction of 223 using Dibal-H at -78°C for overnight and thus a complete conversion was obtained and the alcohol 288 was formed in 98% yield (Scheme 78). With this alcohol 288 in hand, we oxidized it with pyridium chlorochromate (PCC) as a reported method by Pedrosa et al. 148 in DCM at 50°C for 4 hrs. There was some trouble in the purification process from the column chromatography probably the mixture was sensitivity to the silica gel even though we obtain the aldehyde 263 in 44% yield, but there are still some impurities in the compound. So we sought another method and found the report of Nadkarni, et al. 137 with IBX which is a powerful oxidizing agent. The reaction worked well and we isolated the aldehyde 263 in 64% yield in pure form (Scheme 79). Scheme 79 : Oxidation of alcohol 237 With this methodology in hand, there's a need to have substrates of either halide or hydroxyl group in the aromatic ring system, as the first partner for Ullmann-type coupling reaction. The goal is to be able to synthesize the south part of our molecule and this will require the incorporation of mono-halogenated substrates into the aromatic ring system which will enhance the coupling of the diaryl ether.

With the 4-methoxyphenyl acetic acid 224 obtained by saponification of the corresponding ester (as already shown in Scheme 42) in hand, we engage it with oxone as oxidant and saturated NaCl according to M. Antonello et al 149 . Unfortunately, the reaction was unsuccessful. Probably, the compound is not compatible with the oxidant reagent. Thus, we sought for a more efficient method to achieve this and the report by G. Yu et al 150 with sulfuryl chloride in a polar protic solvent (AcOH) at room temperature for 3 hrs gave us a direct mono-chlorination of electron-rich aryl compound 289 in 94% yield (Scheme 80). For the preparation of brominated compound, we reproduced the reaction described with very classic conditions using bromine in acetic acid.

Scheme 81 : Bromination of acid 224

The bromination of acid 224 gave good results but the iodination of the same substrate 224 was more problematic and yielded to inseparable mixture of mono and di-iodinated compound.

We changed our starting material to commercial p-anisaldehyde 259, which was synthesized according to Kraszkiewicz et al. 135 method. After complete reaction, the only mono-iodinated 261 was isolated in 96% yield (Scheme 82).

Having the saturated nitro compound 291 in hand, we tried the Nef-type reaction152 which was expected to transform the nitro group into a carbonyl group under the acidic condition to form the aldehyde 292. But this reaction was unsuccessful, maybe the by-product of nitrous oxide causes the desired product to degrade in the reaction process (Scheme 85).

Scheme 85 : Formation of aryl aldehyde by Nef-type reaction

After several attempts trying to obtain the iodinated partner for Ullmann-type coupling reaction, we were a bit disappointed not to be able to obtain it by an efficient method, and on a large scale. We stopped our investigations here, considering and hoping that the brominated phenylacetate will be reactive enough under copper catalysis to form our desired diaryl ether synthon for the "South part" of the trilobine.

1) South part of trilobine

We proposed the retrosynthesis analysis of the southern part of trilobine and the need is to establish discrimination of the two functions of aldehyde and/or acid for Pictet-Spengler and/or Bischler-Napieralski reaction respectively. The importance of this step is to enable us to perform the two cyclization reactions sequentially and to avoid the formation of the mixture which is always more complicated to purify. Scheme 86 : Retrosynthesis of the southern part of diaryl ether.

d. Preliminary studies

As a model for our diaryl ether, we first tried to couple simple substrate such as phenol 227 and iodoaryl 243. Numerous cupric or cuprous salt were tried, and all results were more or less the same. No drastic difference was observeed, except with tetrakis(acetonitrile)copper hexafluorophosphate, where the crude reaction mixture is cleaner and easier to purify. Yields is also slightly higher.

Scheme 87 : Ullmann type reaction for diaryl ether 293

We use a complex made in the group. The preparation of this cuprous complex is well described in the literature153 , and simple by the addition of hexafluorophosphoric acid to acetonitrile solution of cuprous oxide.

With this diaryl ether compound in hand, it will give access to two possible ways of cyclization either with Bischler-Napieralski or Pictet-Spengler type reactions. So we further reduced the diaryl ether 293 compounds with Dibal-H in THF and it gave the diaryl ether alcohol of compound 294 in 78% yield (Scheme 88) Table 12 : Ullmann type coupling conditions of halobenzoate We also engaged methyl-4-iodophenyl acetate 243 with eugenol 229 under the same protocol and a promising diaryl ether 300 compound was formed in 83% yield (Scheme 91).

Scheme 91 : Ullmann type coupling of iodophenylacetic ester With the diaryl ethers in hand, we engaged them in ozonolysis reaction for the oxidative cleavage of alkene into an aldehyde. We first tried with compound 299 to obtain aldehyde 301 and this reaction was performed with oxygen and dimethyl sulphide. Unfortunately, the desired aldehyde couldn't be isolated in pure form because it was difficult to separate it from the by-product, even after several attempts and several conditions on column chromatography (Scheme 92). Scheme 92 : Ozonolysis reaction So we sought for another method that could be used for the oxidative cleavage of alkene and the report of Dittrich et al154 was applied on the allylic aryl compound 300 to dihydroxylate it with catalytic osmium tetraoxide to form diol, followed by periodate cleavage. Unfortunately, the desired aldehyde 302 could not be isolated rather the traces were observed from the NMR spectra (Scheme 93) Scheme 93 : Osmium reaction Even if we managed to improve the Ullmann-type coupling, this sequence needs further works to be able to discriminate properly both "masked" aldehyde, and optimized the preparation of aldehyde 302, and engaged it in a Pictet-Spengler cyclization. We plan to do this using the "real" south part of the Trilobine, e.i. with a meta-para connection.

For the "real" south part of Trilobine

After the optimization of the Ullmann-type coupling with eugenol, we were committed to the preparation of the "south part" we need for Trilobine. The aryl moiety we need should have the free hydroxyl group in meta position with respect to the alkyl chain. The retrosynthesis of this "south part" is based on Ullmann-type coupling. We still considered that allyl and ester are best groups as masked aldehyde, and can give back the aldehyde function we need for Pictet-Spengler cyclisation. Moreover, the ester can also give easily an acid function if Pictet-Spengler reaction failed, and if Bishler-Napieralski reaction is necessary for one of the isoquinoline moiety preparation. We found that it will be easy to prepare the less substituted aryl with the allyl group. Two different schemes are possible, as we have to use one phenol and one iodoaryl, and both can be substituted by the methoxy group. The synthesis of the 4-allyl iodobenzene 305 began by the formation of the diazonium salt of the iodoaniline 308. Allylation of diazonium salt was performed using allyl bromide in the presence of ferrous salt, in a mixture of DMSO and water 155 . Unfortunately, even if conversion is complete, the crude mixture couldn't be purified due to a very low polarity, and by-product couldn't be separated to the desired iodo allyl compound 305 156 . Scheme 94 : Synthesis of 4-allyl-iodobenzene 305 b. Preparation of allyl phenol. So, we decide to invert the substituents and to prepare hydroxyl allylbenzene 307 157 and an iodo phenylacetate 306 which supports the pending methoxy group. This is supposed to be formed by halogenation of methoxyphenylacetic acid. The brominated derivative 116 is easier to obtain in pure form, as we have already discussed. The preparation of the 4-allyl phenol 307 was done following the same sequence as for 305 i.e. using Heinrich allylation 155 procedure with ferrous salt. We need for that to use amino phenol 310 as a starting material, and we need to protect the hydroxyl group before the formation of the diazonium salt. In 1995, Dai et al. published the selective protection of aminophenol of the nitrogen or of the oxygen, depending on the use or not of a base and using 3acyl-1,3-thiazolidine-2-thione 311 as acylating agent 158 (Scheme 95) Scheme 95 : Selective protection of aminophenol The results were not as selective as they described, furthermore the preparation of the acylating agent 311 was not so easy. We finally found that the simple use of NaH and pivaloic anhydride give a good results, without any reaction on the nitrogen if we use an excess of sodium hydride and if we allow enough time the aminophenol and the base under stirring before the addition of the pivaloic anhydride. The O-Piv aminophenol can be obtained with nearly quantitative yield in a 10g scale. After that, the same sequence that we use for iodoaniline 308 is engaged (Scheme 96). The reaction is not clean as the one observe for the iodoaniline, and little amount of a by-product is observed which was not possible to eliminate.

Scheme 96 : Synthesis of allyl-phenol 315

To finish this sequence, the protected phenol 315 has to be deprotected into free phenol 307, and have to be engaged in a Ullmann-type reaction with bromo derivative 116 (easier to prepare than the iodo counterpart) to conduct the expected diaryl ether 303. Moreover, we need to scale up these three steps (diazonium salt formation, allylation and deprotection) and optimized the purifications process of these steps to be able to study effectively the Ullmann type coupling reaction with these substrates, and after that the oxidative cleavage of the allyl group into aldehyde.

CONCLUSION

During the course of the total synthesis of trilobine, the monomeric unit of benzyltetrahydroisoquinoline alkaloids was accessed through short sequences of the reaction from commercially available and inexpensive starting materials. From this process, different approaches were developed to access the benzyltetrahydroisoquinoline compound. The first aspect was through the Pictet-Spengler reaction, which worked well with the same protecting group of methoxy but showed some limitations on the regioselective aspect because it always reacts in the process of intramolecular cyclization.

Scheme 97 : Synthesis of dibenzo-p-dioxiran system During the course of the total synthesis of Trilobine compound, the monomeric unit of benzyltetrahydroisoquinoline alkaloids was accessed through short sequences of the reaction from commercially available and inexpensive starting materials. From this process, different approaches were developed to access the benzyltetrahydroisoquinoline compound. Relying on the efficiency of compound 286, we developed the polychloroamide compound in other to access the polyhalide monomeric unit of Trilobine, which was unable to complete due to global pandemic holiday. However, the Bischler-Napieralski reaction gave access to the intramolecular cyclization of benzyltetrahydroisquinoline compound but only as traces, and more needs to improve this sequence.

Scheme 99 Bischler-Napieralski on dichlorophenethylamine Our efforts to access the south part of diaryl ether through Ullmann type reaction gave a promising result but to have a selective diaryl ether compound proved to be impossible under oxidative cleavage tested thus preventing us from obtaining the desired trilobine fragment. Scheme 100 : Scheme 91 : Ullmann type coupling of iodophenylacetic ester A. Perspectives

Regioselectivity study of Pictet-Spengler / Bishler-Napieralski

The first aspect was through the Pictet-Spengler reaction, which worked well with the same protecting group of methoxy but showed some limitations on the regioselective aspect because it always reacts in the process of intramolecular cyclization. Scheme 101 : Regioselective intramolecular cyclization by Pictet-Spengler type

Optimization of Ullmann-type coupling reaction

Our efforts to access the south part of diaryl ether through Ullmann type reaction gave a promising result but to have a selective diaryl ether compound proved to be impossible under oxidative cleavage tested thus preventing us from obtaining the desired Trilobine fragment. For the preparation of the "south part" of trilobine we need to improve the preparation of allylphenol 307 and study the Ullmann type coupling with these two synthons.

B. Characterizations of synthetized compounds

Methyl-4-methoxyphenyl acetate (223)

To a mixture of compound 172 (2.0 g, 13.15 mmol), K2CO3 (6.4 g, 46.0 mmol) in acetone (40 ml), Me2SO4 (2.5 ml, 26.29mmol) was added, and the mixture was stirred overnight at 25°C. The reaction mixture was monitored by TLC silica gel with Cy/EtOAc : 8/2) on the complete conversion of the starting material, and filter through celite ® with EtOAc. The organic phase was washed with brine, dried over Na2SO4, and purified by chromatography over silica gel column to yield to a clear orange oil (2.3 g, 97%).

4-hydroxylphenyl acetate (227)

From the commercially available C8H8O3 (5.0 g, 32.99 mmol) in MeOH (40 ml) and slow addition of SOCl2 (3.0 ml, 39.59 mmol) to stir at 90 o C for 3 hours. Then, the reaction mixture was monitored by TLC silica gel with (Cy/ EtOAc; 8/2). On the complete conversion of the starting materials, without extraction it was evaporated as solution of NaHCO3 was added and extracted with EtOAc. The organic phase was washed with brine, anhydrous Na2SO4 and reduced under pressure, and the crude mixture was further purified by column chromatography to obtain a white solid (5.3 g, 96%).

4-methoxyphenyl acetic acid (224)

To the mixture of compound 223 (2.1 g, 11.78 mmol) in 10 ml of MeOH/H2O (1/1) was added LiOH (1.0g, 23.56 mmol) and the reaction was stirred at 55 o C for an hour. Then, heptane (10ml) was added and the stirring was kept for an additional hour. The reaction mixture was monitored by TLC silica gel with (DCM/MeOH : 9/1) on the complete conversion of the starting material, it was quenched and separated from it aqueous layer. While, the organic phase was acidified with 10% citric acid to a pH 4 and was further extracted with EtOAc, washed with brine, dried over Na2SO4 and concentrated to afford white solid (1.7 g, 86%). Rf = 0. 78 (EtOAc, 100%) 1 H NMR (300 MHz, CDCl3) δ (ppm): 7.20 (d, J = 8.8 Hz, 2H), 6.87 (d, J = 8.7 Hz, 2H), 3.80 (s, 3H), 3.59 (s, 2H).

3-chloro-4-methoxyphenyl acetic acid (289)

A mixture of acid 224 (0.31 g, 1.84 mmol) in AcOH (1 ml) was stirred for 10 min and SO2Cl2 (0.2 ml, 1.84 mmol) was added dropwise to continue stirring at 25 o C for 3 hours. The reaction mixture was monitored by TLC silica gel with EtOAc (100%), on complete conversion of the starting material. The solvent was then evaporated under reduced pressure, and NaHCO3 was added at 0°C for neutralisation, followed by excess of a 5% solution of Na2SO3 at the same temperature. The precipitate was washed with ice H2O, dried overnight on sinteredglass and recrystallized from DCM to form white solid (0.35 g, 94%). Rf = 0.22 (EtOAc, 100%)

3-bromo-4-methoxyphenyl acetic acid (225)

A mixture of acid 224 (1.04 g, 6.24 mmol) in AcOH (2 ml) was stirred for 10 min and Br2 (0.8 ml, 6.86 mmol) was added dropwise to continue stirring at 25 o C for 3 hours. The reaction mixture was monitored by TLC silica gel with EtOAc (100%), on complete conversion of the starting material, it was quenched with a solution of 5% Na2SO3 at 0 o C. The precipitate was washed with ice H2O, dried overnight on sinteredglass and recrystallized with DCM to form white solid (0.95 g, 63%). SOCl2 (0,4 ml, 5,77 mmol) was slowly added to a mixture of acid 225 (1.18 g, 4.81 mmol) in MeOH (10 ml) and the mixture was stirred for 3 hours at 90°C. The reaction mixture was monitored by TLC (Cy/EtOAc : 8/2) on complete conversion of the starting material, and solvants were evaporated. A solution of NaHCO3 was added for neutralisation and then compound was extracted with DCM. The organic phase was washed with brine, dried over Na2SO4 and concentrated under reduced pressure. Purification by chromatography over silica gel column yielded to an orange oil (1.24 g, 99%). Rf = 0.58 (Cy/EtOAc ; 90/10)

3-bromo-4-methoxyphenyl aldehyde (320)

To a solution of morpholine (0.4 ml, 4.9 mmol) in THF (10 ml) was added Dibal-H (1M THF; 9.0 ml, 9.0 mmol) and stirred at 0°C for 3 hrs. Then, the solution C10H11BrO3 (0.64 g, 2. 45 mmol) was added dropwise to the mixture at 0°C to continue stirring overnight. The reaction mixture was monitored by TLC (Cy/ EtOAc; 8/2) on complete conversion of the starting material, it was quenched with 1N HCl and extracted with EtOAc, then treat with brine, Na2SO4 and reduced under pressure. The crude mixture was further purified by column chromatography to form white solid (0.38 g, 67%). Rf = 0.59 (Cy/EtOAc: 85/15)

4-iodophenyl alcohol (244)

To a solution of acid 240 (1.57 g, 5.99 mmol) in THF (15 ml), 13 ml of a 1M solution of borane in THF (13 mmol) was added at 0°C and the mixture was stirred overnight. The reaction was monitored by TLC, quenched with H2O and extracted with EtOAc, the organic phase was washed with brine, dried over Na2SO4 and concentrated under reduced pressure and recrystallized from EtOH to form white solid (1.44 g, 97%). Rf = 0.26 (EtOAc 100%) 1 H NMR (300 MHz, CDCl3) δ (ppm): 7.69 -7.55 (m, 2H), 6.99 (d, J = 8.3 Hz, 2H), 3.84 (t, J = 6.5 Hz, 2H), 2.81 (t, J = 6.5 Hz, 2H).

4-iodophenyl phthalimide (246)

To a solution of alcohol 244 (1.82 g, 7.36 mmol) in THF (15 ml) were added P(Ph)3 (3.86 g, 14.73 mmol) and phthalimide (2.17 g, 14.73 mmol) and the mixture was stirred 10 minutes at 0°C. Then, DEAD (2.56 g, 14.73 mmol) was added dropwise and the reaction was stirred overnight and quenched with water. Products were extracted with DCM, and organics phases were dried over Na2SO4 and concentrated under reduce pressure. Recristallisation from EtOH give phthalimide 246 (2.67 g, 96%).

Rf

3-iodo-4-methoxybenzy-β-nitrostyrene (290)

A solution of aldehyde 261 (0.58 g, 2.23 mmol) and NH4OAc (0.43 g, 5.53 mmol) in AcOH (10 ml) was stirred for 10 mins at 25°C, then MeNO2 (0.5 ml, 8.92 mmol) was added and the mixture was heated at 125°C for 3 h. The reaction was monitored by TLC (Cy/DCM : 8/2), quenched in ice bath by adding water and extract with DCM. The organic layer was washed with brine, dried over Na2SO4, concentrated under reduced pressure, and purified by chromatography over silica gel column yielded to a yellow solid (0.66 g, 96%).

3-iodo-4-methoxyethylnitrobenzene (291)

To a solution of 290 (0.42 g, 1.36 mmol) in 10 ml of a miwture of DCM/MeOH (1/1) at 0°C, was slowly added NaBH4 (0.077 g, 2.046 mmol) and the reaction was stirred for 2 h. The reaction was monitored by TLC (Cy/DCM : 8/2), quenched in ice bath by addition of water and extracted with DCM. The organic layer was washed with brine, dried over Na2SO4, concentrated under reduced pressure, and purified by chromatography over silica gel column yielded to a yellow solid (0.26 g, 62%). Rf = 0.56 (Cy/ DCM; 80/20)

3,4-dichlorophenyl-β-nitrostyrene (257)

A solution of aldehyde 256 (0.64 g, 3.65 mmol) and NH4OAc (0.84 g, 10.94 mmol) in AcOH (10 ml) was stirred for 10 mins at 25°C, then follow by addition of MeNO2 (0.8 ml, 14.96 mmol) to reflux at 125°C for 3 h. Monitored by TLC (Cy: DCM; 8:2), quenched in ice bath by adding water and extract with DCM. The organic layer was washed with brine, dried over Na2SO4, concentrated under reduced pressure, and purified by chromatography over silica gel column yielded to a yellow solid (0.72 g, 90%).

Rf

3,4-dichlorophenylethylamine (237)

A 1M solution of DiBAl-H (16 ml, 16 mmol) in THF (15 ml) was heated to reflux under nitrogen, then a solution of compound C8H5Cl2NO2 (153 mg, 0.702 mmol) in THF (15 ml) was slowly added to the reaction mixture and kept to reflux for 3 h. The reaction was quenched in the following: brought to -78°C and slowly add H2O (2 ml) and stir for 10 mins, the cooling bath was removed and aqueous. 5% NaOH (2 ml) was added. More H2O (2 ml) and diethyl ether were added to keep the reaction stirring and excess dried Na2SO4 was added to form cake which was filtered and wash with diethyl ether to form light-brown oil (129 mg, 97%). Rf = 0.21 (EtOAc 100%)

N-Boc-3,4-dichlorophenylethylamine (234)

A mixture of 3,4-dichlorophenylethylamine 237 (1.0 g, 5.26 mmol) and di-tert-butyl dicarbonate (1.37 g, 6.31 mmol) in MeOH/Et3N (9:1) were heated to reflux for 45 mins. The The reaction was monitored by TLC, quenched with water and concentrated. The residue was treated with 1N HCl to pH 2, then extracted with DCM, and the organic layer was washed with brine, dried over Na2SO4, concentrated under reduced pressure, and purified by chromatography over silica gel column yielded to a white solid colour (1.45 g, 95%). Rf = 0.45 (Cy/EtOAc : 90/10)

7,12-dimethyl-15-Nor-coclaurine (260)

To a solution of compound 258 (0.061 g, 0.22 mmol) and aldehyde 259 (0.05 ml, 0.43 mmol) in 10 ml of DCM/CHCl3 (1/1) and was slowly added BF3-OEt2 (0.05 ml, 0.43 mmol) at -78°C and the mixture was stirred overnight. The reaction was monitored by TLC (DCM/MeOH : 9/1), quenched with a saturated aqueous solution of NaHCO3 and extracted with EtOAc. The organic layer was washed with brine, dried over Na2SO4, concentrated under reduced pressure, and purified by chromatography over silica gel column yielded to an orange solid (0.059 g, 91%).

Rf

3-iodo-7,12-dimethyl-15-Nor-coclaurine (262)

To a solution of compound 258 (0.24 g, 0.88 mmol) and aldehyde 261 (0.46 g, 1.74 mmol) in 10 ml of DCM/CHCl3 (1/1) and was slowly added BF3-OEt2 (0.2 ml, 1.74 mmol) at -78°C and the mixture was stirred overnight. The reaction was monitored by TLC (DCM/MeOH : 9/1), quenched with a saturated aqueous solution of NaHCO3 and extracted with EtOAc. The organic layer was washed with brine, dried over Na2SO4, concentrated under reduced pressure, and purified by chromatography over silica gel column yielded to an orange solid (0.29 g, 78%). Rf = 0.27 (DCM/MeOH : 95/5)

7,12-dimethyl-coclaurine (264)

To a solution of compound 258 (0.24 g, 0.85 mmol) and aldehyde 261 (0.26 g, 1.71 mmol) in 10 ml of DCM/CHCl3 (1/1) and was slowly added BF3-OEt2 (0.22 ml, 1.71 mmol) at -78°C and the mixture was stirred overnight. The reaction was monitored by TLC (DCM/MeOH : 9/1), quenched with a saturated aqueous solution of NaHCO3 and extracted with EtOAc. The organic layer was washed with brine, dried over Na2SO4, concentrated under reduced pressure, and purified by chromatography over silica gel column yielded to an orange solid (0.101 g, 39%). To a solution of compound 293 (0.34 g, 1.09 mmol) in 10 ml of MeOH/H2O (1/1) was added LiOH (0.18 g, 4.39 mmol) and the mixture was stirred at 55 o C for an hour. Then, heptane (10ml) was added and the reaction was kept stirring for another hour. The reaction mixture was monitored by TLC with (DCM/MeOH : 9/1) on the complete conversion of the starting material, it was quenched and separated from it aqueous layer. While, the organic phase was acidified with 10% citric acid to a pH 4 and was further extracted with EtOAc, washed with brine, dried over Na2SO4 and concentrated to afford white solid (0.23 g, 72%).

Diphenyl ether alcohol (294)

To the mixture of C18H20O5 (0.29 g, 0.925 mmol) in THF (10 ml) brought to -78°C, DiBAl-H (1M; 3.0 ml, 1.25 mmol) was slowly added and the reaction was stirred overnight. The reaction was monitored by TLC (Cy/EtOAc : 8/2), quenched with 1N HCl and extracted with DCM. The organic layer was washed with brine, dried over Na2SO4, concentrated under reduced pressure, and purified by chromatography over silica gel column to yield to an orange-brown gummy solid (0.21 g, 86%). Rf = 0.25 (Cy/EtOAc : 85/15) IR vmax (cm -1 ) = 3122, 2921, 1596, 1444, 1227 1 H NMR (300 MHz, CDCl3) δ 7.12 -7.07 (m, 4H), 6.80 -6.76 (m, 4H), 3.83 (t, J = 6.5 Hz, 4H), 2.81 (t, J = 6.5 Hz, 4H).

Dibenzo-p-dioxine nucleus phenylethylamine (236)

A mixture of compound 234 (86 mg, 0.30 mmol), compound 235 (0.75 mg, 0.30 mmol) and K2CO3 (82m g, 0.60 mmol) in DMF (1.5 ml) was heated to reflux at 180°C for 24 h. Monitored by TLC (DCM/MeOH : 9/1), quenched with H2O and extracted with EtOAc. The organic layer was washed with brine, dried over Na2SO4, concentrated under reduced pressure, and purified by chromatography over silica gel column to yield to 61 mg (21%) of compound 236 Rf = 0.25 (DCM/MeOH : 90/10) IR vmax (cm -1 ) = 3305, 2925, 1620, 1468, 1131 1 H NMR (300 MHz, CDCl3) δ (ppm): 7.37 (d, J = 8.2 Hz, 2H), 7.27 (d, J = 2.0 Hz, 2H), 7.02 (dd, J = 8.2, 2.0 Hz, 2H), 3.40 (t, J = 6.9 Hz, 4H), 2.77 (t, J = 6.9 Hz, 4H).

Compound 300

A mixture of compounds 243 (0.199 g, 0.72 mmol), 229 (0.177 mg, 1.08 mmol), with Cs2CO3 (0.47 g, 1.44 mmol) and 5 mol% of Cu(MeCN)4.PF6 (0.014 g, 0.036 mmol) in xylene (0.8 ml) was heated at 120°C for 48 hours. The reaction was monitored by TLC (Cy/EtOAc : 8/2), quenched with water and extracted with EtOAc and concentrated. The residu was purified by chromatography over silica gel column yielded to a brown gum (0.19 g, 83%). Rf = 0.28 (Cy/EtOAc : 85/15) IR vmax (cm -1 ) = 3062, 1711, 1504, 1419, 1226 1 H NMR (300 MHz, CDCl3) δ (ppm): 7.23 -7.14 (m, 2H), 6.93 -6.85 (m, 3H), 6.82 (d, J = 1.9 Hz, 1H), 6.74 (dd, J = 8.1, 2.0 Hz, 1H), 5.99 (ddt, J = 16.9, 10.2, 6.7 Hz, 1H), 5.15 -5.08 (m, 2H), 3.81 (s, 3H), 3.61 (s, 2H), 3.39 (d, J = 6.7 Hz, 2H).

Diphenyl ether (299)

A mixture of compounds 296 (0.15 g, 0.57 mmol), 229 (0.701 mg, 1.08 mmol), with Cs2CO3 (0.37 g, 1.14 mmol) and 5 mol% of Cu(MeCN)4.PF6 (0.011 g, 0.028 mmol) in xylene (0.8 ml) was heated at 120°C for 8 days. The reaction was monitored by TLC(Cy/EtOAc : 8/2), quenched with water and extracted with EtOAc and concentrated. The residu was purified by chromatography over silica gel column yielded to a brown gum (0.11 g, 65%).

N-Boc dopamine (235)

A solution of dopamine chlorhydrate 7*HCl(4.01 g, 21.15 mmol) and di-tert-butyl dicarbonate (5.54 g, 25.37 mmol) in MeOH/Et3N (9/1) was heated to reflux for 45 mins. The reaction was monitored by TLC (DCM/MeOH : 9/1), quenched with water and concentrated under reduced pressure. The residue was treated with 1N HCl to pH 2, then extracted with EtOAc, washed with brine, dried over Na2SO4, concentrated and purified by chromatography over silica gel column to yield to a white solid (5.03 g, 94%). Rf = 0.65 (DCM/MeOH : 95/5) IR vmax (cm -1 ) = 3366, 2978, 1682, 1447 1 H NMR (300 MHz, CDCl3) δ 6.78 (d, J = 8.0 Hz, 1H), 6.69 (s, 1H), 6.56 (d, J = 7.9 Hz, 1H), 3.31 (d, J = 6.2 Hz, 2H), 2.64 (t, J = 6.9 Hz, 2H), 1.44 (s, 9H). HRMS m/z calcd for C13H18O4N [ESI] -: 252.12389, found: 252.12413

N-boc-3,4-dimethoxyphenylethylamine (258)

A mixture of compound 235 (3.8 g, 15.02 mmol), K2CO3 (7.26 g, 52.51 mmol) and C2H6SO4 (2.8 ml, 30.01 mmol) in acetone (30 ml) were stirred overnight at 25°C. The reaction was monitored by TLC (Cy/EtOAc : 8/2), filtered through Celite ® with EtOAc. The organic phase was washed with brine, dried over Na2SO4, concentrated and was purified by chromatography over silica gel column to yield to a white solid (3.72 g, 88%). Rf = 0.54 (Cy/EtOAc : 90/10) IR vmax (cm -1 ) = 3374, 2975, 2836, 1608, 1142. 1 H NMR (300 MHz, CDCl3) δ 6.85 (d, J = 8.1 Hz, 1H), 6.78 (d, J = 1.9 Hz, 1H), 6.75 (s, 1H), 3.90 (d, J = 3.2 Hz, 6H), 3.39 (dd, J = 13.1, 6.6 Hz, 2H), 2.78 (t, J = 7.0 Hz, 2H), 1.47 (s, 9H). To a mixture of compound 271 (5.57 g, 16 mmol) in acetone (50 ml) were added K2CO3 (7.73 g, 55.9 mmol) and Me2SO4 (1.5 ml, 16 mmol) and the reaction was stirred at 25°C for 16 h. The reaction was monitored by TLC (Cy/EtOAc : 8/2), quenched and filtered through a pad celite and wash with EtOAc, the organic layer was washed with brine, dried over Na2SO4, concentrated under reduced pressure and purified by chromatography over silica gel column to yield to a white solid (5.57 g, 96%). To a mixture of compound 272 (5.36 g, 14. 81 mmol) in THF (20 ml) under nitrogen was brought to -78°C and Dibal-H (1.0M; 18.0 ml, 18mmol) was added dropwise and the reaction was stirred overnight. Monitored by TLC (Cy/EtOAc : 8/2), quenched at 0°C by slowly addition of water. Then, extracted with EtOAc and the organic layer was washed with water, brine, dried over Na2SO4 and concentrated under reduced pressure, and purified by chromatography over slica gel column yielded to a white solid (3.89 g, 79%). To a solution of compound 273 (3.89 g, 11.62 mmol) in DMSO (1 ml) was added IBX (4.89 g, 17.43 mmol) and the mixture was stirred at 45°C for 2 h. The reaction was monitored by TLC (Cy/EtOAc : 8/2), quenched with water and filtered with EtOAc. The organic layer was washed with brine, dried over Na2SO4 and concentrated under reduced pressure, which was further purified by chromatography over silica gel column to yield to a white solid (2.81 g 73%).

Rf = 0.53 (Cy/EtOAc : 90/10) IR vmax (cm -1 ) = 2844, 1691, 1507, 1326, 1128 1 H NMR (300 MHz, CDCl3) δ (ppm): 9.78 (s, 1H), 7.59 (ddd, J = 6.9, 3.3, 1.5 Hz, 4H), 7.41 -7.36 (m, 6H), 7.12 (dd, J = 3.4, 1.4 Hz, 2H), 3.98 (s, 3H). A solution of compound 274 (2.52 g, 7.58 mmol) and NH4OAc (1.75 g, 22. 74 mmol) in AcOH (20 ml) was stir for 10 min at 25°C, then nitromethane (1.6 ml, 31.0743 mmol) was added, and the mixture was heated to reflux at 130°C for 4 h. The reaction is monitored by TLC (Cy/DCM : 8/2), cooled with ice bath and quenched with water, extracted with DCM. The organic phases was washed with brine, dried over Na2SO4, concentrated under reduced pressure and purified by chromatography over silica gel column to yield to a yellow solid (2.094 g 74%). A solution of amine 276 (0.34 g, 0.99 mmol) and di-tert-butyl dicarbonate (0.26 g, 1.19 mmol) in a mixture of MeOH and Et3N (9/1) was heated to reflux for 45 min. The reaction was monitored by TLC (Cy/EtOAc : 8/2), and concentrated. The residue was treated with 1N HCl to pH 2 then extracted with DCM, brine dried over Na2SO4 and concentrated and purified by chromatography over silica gel column to yield to a white solid (0.199 g, 46%).

3,3',4-trichloro-4'-methoxyphenylethylamide (287)

Mixture of dichlorophenethylamine 237 (0.14 g, 0.85 mmol) and acid 287 (0.17 g, 0.85 mmol) was heated neat for 2 hours at 190°C. The reaction was purified by chromatography over silica gel column, yielded a brown solid (0.19 g, 59%).

To a solution of ester 227 (1.0 g, 5.61 mmol) in THF (10 ml) at -78°C, was added slowly 13 ml of a 1M solution of DIBAL in THF (1M; 13.0 ml, 13 mmol) and the reaction was stirred overnight. Monitored by TLC (Cy/EtOAc : 8/2), quenched with 1N HCl and extracted with CH2Cl2. The organic layer was washed with brine, dried over Na2SO4 and concentrate under reduced pressure, and was further purified by chromatography on silica gel column, yielded to a white solid (0.76 g, 89%). Rf = 0.26 (Cy/ EtOAc; 90/10) 1 H NMR (300 MHz, CDCl3) δ (ppm): 7.15 (d, J = 8.7 Hz, 2H), 6.86 (d, J = 8.7 Hz, 2H), 3.83 (d, J = 3.9 Hz, 2H), 3.80 (s, 3H), 2.82 (t, J = 6.5 Hz, 2H).

3,4-diiodobenzyl alcohol (252)

To a solution of acid 250 (3.0 g, 8.023 mmol) in THF (20 ml) at -78°C and a 1M solution of borane tetrahydrofuran complex (1M; 17.0 ml, 17 mmol) was slowly added and the mixture was stirred overnight. The reaction was monitored by TLC (Cy/EtOAc : 8/2), quenched slowly with water and extracted with DCM. The organic layer was washed with brine, dried over Na2SO4, concentrated under reduced pressure, and was further purified by chromatography on silica gel column, yielded to a white solid (2.63 g, 91%). Rf = 0.25 (Cy/ EtOAc; 90 /10)

Figure 1 :

 1 Figure 1 : Structure of trilobine and isotrilobine

Figure 2 :

 2 Figure 2 : Example of true alkaloids

Figure 3 :Figure 4 :

 34 Figure 3 : Example of proto alkaloids

Figure 5 :

 5 Figure 5 : Skeletal structure and numeration of isoquinoline alkaloids

Figure 6 :

 6 Figure 6 : Skeletal structure of benzyltetrahydroisoquinoline alkaloids

Figure 7 :

 7 Figure 7 : Structural types of bisbenzyltetrahydroisoquinoline alkaloids

Figure 8 :

 8 Figure 8 : Numbering of bisbenzyltetrahydroisoquinoline alkaloids

Scheme 1 :

 1 Scheme 1 : Two putative biosynthetic routes to norlaudanosoline skeleton by Schumacher et al

Figure 9 :

 9 Figure 9 : Stereochemical inversion of coclaurine and reticuline

Figure 10 :

 10 Figure 10 : Numerotation of BBIQ

Figure 12 :

 12 Figure 12 : Structure of BBIQ isolated by Giannis et al.

Figure 13 :

 13 Figure 13 : Structure of Nelumborine

Figure 15 :

 15 Figure 15 : A novel drug of malarial by Chaturvedi et al.

  53 D.Chaturvedi, A. Goswami, P. P. Saikia, N. C. Barua and P. G. Rao. Chem. Soc. Rev. 2010, 39, 435 -454. 54 M. Del Rayo Camacho, J. D. Phillipson, S. L. Croft, P. Rock, S. J. Marshall and P. L. Schiff Jr. Phytother. Res. 2002, 16(5), 432 -436.

Figure 16 :

 16 Figure 16 : Structures of bisbenzyltetrahydroisoquinoline by Camelo et al.

Figure 17 :

 17 Figure 17 : Cocculus hirsutus plant and Raw & ripened fruits by Dinesh Valke photo

  58 J.R. Merchant, R.M. Naik, S.N. Hirwe. J. India Chem. Soc. 1962, 39, 411 -416. 59 J.E. Caius. The medicinal and poisonous plants of India, Jodhpur, scientific publisher; 1986, 166 -171. 60 T.K Chatterjee. Herbal options, 1 st ed M/s Eastern Traders, Calcutta; 1996, 157 -178. 61 S. Sengottuvelu, K. Rajesh, S. H. Sherief, R. Duraisami, M. Vasudevan, J. Nandhakumar, D. Karthiakeyan and T. Sivakumar. J. Res. Educ. Indian Med. 2012, 18(3), 175 -182.

Figure 18 :

 18 Figure 18 : Structure of isolated phytoconstituents from the leaves of C. hirsutus

  B. W. Stewart and C. P. Wild. World cancer report, 2014. IARC Nonserial 2014.analogues of the vinca alkaloids have been synthesized and then entered into clinical use including79 vindesine 98, vinorelbine 99, and vinflunine 100 (Figure20).

Figure 20 :

 20 Figure 20 : Anticancer drug from vinca alkaloids

Figure 22 :

 22 Figure 22 : Anticancer drug from podophyllotoxine alkaloids by Canel et al All these natural products had represented important milestones in the anti-cancer drugs development with their specific mode of action, and BBIQs extracted from Menispermaceae such trilobine and isotrilobine may represente next one with development of knowledges in the epigenetic field.

Figure 24 :

 24 Figure 24 : Structure of Trilobine 1 and Obaberine 41

Scheme 6 :

 6 Scheme 6 : Ullmann type reaction

Scheme 12 :

 12 Scheme 12 : Oxidation of bisbenzyltetrahydroisoquinoline alkaloids by Shamma et al

  reported the synthetic work of bisbenzylisoquinoline-type alkaloids by alkylation of the anion of isoquinoline with bifunctional diaryl ether 142. The sequence involving diaryl ether 142 and the Reissert 143 derivatives afford bisbenzylisoquinoline 144. The quaternarization of deprotected 144 with methyl iodide and subsequent sodium borohydride reduction gave O-Methyldauricine 145 in racemic form (Scheme 15). 97 A. Reissert, Chem. Ber. 1905, 38, 1603-1614. 98 G. Blasko, P. Kerekes and S. Makleit. Alkaloids, (Academic Press) 1987, 31, 1 -28.

1 .

 1 Scheme 17 : Synthesis of O-methyldauricine alkaloids by Tomita et al.

  Scheme 20 : Synthesis of Liensinine by Kametani et al.

Figure 25 :

 25 Figure 25 : Cepharanthine structure

Scheme 24 :

 24 Scheme 24 : Synthesis of diphenyl ether diacetic acid mono-ester by Tomita et al.

Figure 26 :

 26 Figure 26 : Structure type VIII BBIQs

Scheme 29 :

 29 Scheme 29 : Synthesis of Isotetrandrine and Pheanthine alkaloids by Inubushi et al.

  116 M. Tomita, H. Furukawa, T. Kikuchi, A. Kato, T. Ibuka. Chem. Pharm. Bull., 1966, 14, 232 -237. 117 Y.Liu, L. Harinantenaina, P.J. Brodie, C. Slebodnick, M.W. Callmander, R. RAkotondrajaona, E. Rakotobe, V.E. Rsamison, K. TenDyke, Y. Shen, D.G.I. Kingston, Magn. Reson. Chem. 2013, 51, 574 -579.

Figure 28 :

 28 Figure 28 : X-ray of N-Cocsuline 218 (type XXIII) by Kinston et al.

Figure 29 :

 29 Figure 29 : mass spectra of isotetrandrine by Tomita et al

Figure 30 :

 30 Figure 30 : NMR spectroscopy of Laudanosine alkaloids

Figure 31 :

 31 Figure 31 : Retrosynthesis of trilobine and isotrilobine alkaloids

Scheme 44 :

 44 Scheme 44 : Copper catalyzed coupling reaction of arylboronic acid and eugenol

  Scheme 59 : Synthesis of dimethoxyphenylethylamine 258

Scheme 78 :

 78 Scheme 78 : Reduction of ester 223

Scheme 80 :

 80 Scheme 80 : Direct mono-chlorination of 224

Figure 34 :

 34 Figure 34 : « South part » for Trilobine

Figure 35 :

 35 Figure 35 : Retrosynthetic scheme for the south part of Trilobine

Scheme 98 :

 98 Scheme 98 : Synthesis of THIQ via Pictet-Spengler reaction

Rf = 0. 47 (

 47 Cy/ EtOAc: 90/10) IR vmax (cm -1 ) = 2998, 1738, 1613, 1437.29, 1301 1 H NMR (CDCl3, 300 MHz): δ (ppm) = 7.20 (d, J = 8.8 Hz, 2H), 6.86 (d, J = 8.7 Hz, 2H), 3.79 (s, 3H), 3.68 (s, 3H), 3.57 (s, 2H). 13 C NMR (75 MHz, CDCl3) δ 172.4 (CO), 158.7 (Cq), 130.3 (2*CH), 126.1 (Cq), 114.2 (2*CH), 55.3 (CH3), 52.1 (CH3), 40.3 (CH2). HRMS m/z calcd for C10H12O3 [ESI] + : 203.06769, found: 203.06787

  Rf = 0.64 (Cy/EtOAc; 85/15) IR vmax (cm -1 ) = 3376 (OH), 1720 (C=O), 1615 (C=C), 1444 (CH2), 1348 (C-O), 1233 (CH3).

1 H

 1 NMR (300 MHz, CDCl3) δ (ppm): 7.13 (d, J = 8.5 Hz, 2H), 6.76 (d, J = 8.5 Hz, 2H), 3.70 (d, J = 1.6 Hz, 3H), 3.56 (s, 2H).

13 C

 13 NMR (75 MHz, CDCl3) δ (ppm): 172.8 (CO), 154.9 (Cq), 130.6 (2C), 125.8 (Cq), 115.5 (2C), 52.1 (CH3), 40.9 (CH2).

13 C

 13 NMR (75 MHz, CDCl3) δ (ppm): 178.1 (CO2H), 158.8 (Cq), 130.4 (2*CH), 125.3 (Cq), 114.1 (2*CH), 55.3 (CH3), 40.3 (CH2). HRMS m/z calcd for C9H9O3 [ESI] -: 165.05555, found: 165.05572

1 H

 1 NMR (300 MHz, CDCl3) δ (ppm): 7.31 (d, J = 2.2 Hz, 1H), 7.15 (dd, J = 8.4, 2.2 Hz, 1H), 6.89 (d, J = 8.4 Hz, 1H), 3.89 (s, 3H), 3.58 (s, 2H).

13 C

 13 NMR (75 MHz, CDCl3) δ (ppm): 176.1 (CO2H), 131.4 (Cq), 130.4 (CH), 128.6 (C-Cl), 126.2 (CH), 114.1 (Cq), 112.2 (CH), 56.2 (CH3), 39.6 (CH2).

  Rf = 0.21 (EtOAc, 100%) IR vmax (cm -1 ) = 3029, 1712, 1502, 1401, 1234, 1054, 590 1 H NMR (300 MHz, CDCl3) δ (ppm): 7.48 (d, J = 2.2 Hz, 1H), 7.19 (dd, J = 8.4, 2.2 Hz, 1H), 6.86 (d, J = 8.4 Hz, 1H), 3.88 (s, 3H), 3.58 (s, 2H).

13 C

 13 NMR (75 MHz, CDCl3) δ (ppm): 177.6 (CO2H), 155.2 (Cq), 134.2 (CH), 133.6 (Cq), 129.5 (CH), 126.7 (Cq), 111.8 (CH), 56.3 (CH3), 39.6 (CH2). HRMS m/z calcd for C9H8O3Br [ESI] -: 242.96617, found: 242.96623 3-bromo-4-methoxyphenyl acetate (116)

13 C

 13 NMR (76 MHz, CDCl3) δ 138.2 (2*CH), 137.6 (Cq), 131.1 (2*CH), 91.7 (Cq), 63.4 (CH2), 38.6 (CH2). HRMS m/z calcd for C8H9IO: [ESI] + : 247.96943, found: 247.96981

13 C

 13 = 0.59 (Cy/EtOAc; 90/10) 1 H NMR (300 MHz, CDCl3) δ (ppm): 7.83 (dd, J = 5.5, 3.0 Hz, 2H), 7.71 (dd, J = 5.5, 3.1 Hz, 2H), 7.60 (d, J = 8.3 Hz, 2H), 7.00 (d, J = 8.3 Hz, 2H), 3.94 -3.86 (m, 2H), 2.98 -2.90 (m, 2H). 2* NMR (75 MHz, CDCl3) δ (ppm): 168.1 (2*CO), 160.3 (2*Cq), 137.6 (2*CH), 134.0 (2*CH), 132.0 (Cq), 130.9 (2*CH), 123.3 (2*CH), 92.0 (Cq), 65.5 (CH2), 14.1 (CH2). HRMS m/z calcd for C17H16INO3: [ESI] + : 432.00602, found: 432.00671 4-iodophenylethylamine (247) To a solution of compound 246 (2.45 g, 6.51 mmol) in MeOH (2 ml), N2H4 (1.5 ml, 48.72 mmol) was added and the mixture was heated at 65°C for 2 h. The reaction was monitored by TLC (DCM/MeOH : 9/1), evaporated to dryness to form white solid colour (1.53 g, 95%) Rf = 0.21 (DCM/MeOH : 95/5) 1 H NMR (300 MHz, CDCl3) δ (ppm): 7.62 (d, J = 8.2 Hz, 2H), 6.96 (d, J = 8.2 Hz, 2H), 2.96 (t, J = 6.9 Hz, 2H), 2.70 (t, J = 6.8 Hz, 2H). . HRMS m/z calcd for C8H11NI: [ESI] + : 247.99280, found: 247.993073-iodo-4-methoxybenzaldehyde (261)NaIO4 (0.61 g, 2.81 mmol) and KI (1.4 g, 8.43 mmol) were added slowly to concentrated. H2SO4 (95%, 30 ml) under stirring at 25°C and the mixture was stirred for 45 mins forming a dark brown solution. Then, a commercial p-anisaldehyde (1.2 ml, 10.0 mmol) was added dropwise to the stirring solution for overnight. The mixture was slowly poured into a stirred ice H2O, the crude was collected by filtration with continuous washing with H2O until the filtrate were neutral. Dried on sintered glass and it forms light-brown solid (2.42 g, 93%). Rf = 0.49 (Cy/EtOAc; 80/20) 1 H NMR (300 MHz, CDCl3) δ (ppm): 9.82 (s, 1H), 8.31 (d, J = 2.0 Hz, 1H), 7.86 (dd, J = 8.5, 2.0 Hz, 1H), 6.92 (d, J = 8.5 Hz, 1H), 3.98 (s, 3H). 13 C NMR (75 MHz, CDCl3) δ (ppm): 189.4 (CO), 162.8 (CO), 141.1 (CH), 132.1 (Cq), 131.4 (CH), 110.6 (CH), 86.5 (I), 56.8 (CH3)

Rf = 0

 0 .60 (Cy/DCM : 80/20) 1 H NMR (300 MHz, CDCl3) δ (ppm): 7.99 (d, J = 2.2 Hz, 1H), 7.89 (d, J = 13.6 Hz, 1H), 7.56 -7.46 (m, 2H), 6.87 (d, J = 8.6 Hz, 1H), 3.95 (s, 3H). HRMS m/z calcd for C21H15O5 [ESI] -: 304.95408, found: 304.95489

  = 0.7 (Cy/DCM; 80/20) 1 H NMR (300 MHz, CDCl3) δ (ppm): 7.91 (d, J = 13.7 Hz, 1H), 7.64 (d, J = 2.1 Hz, 1H), 7.56 (d, J = 3.9 Hz, 1H), 7.53 (d, J = 1.4 Hz, 1H), 7.38 (dd, J = 8.3, 2.1 Hz, 1H). 13 C NMR (75 MHz, CDCl3) δ(ppm): 138.3 (Cq), 136.4 (CH), 134.0 (Cq), 131.5 (CH), 130.6 (CH), 130.0 (Cq), 127.9 (CH), 111.5 (CH). HRMS m/z calcd for C8H5Cl2O2N [ESI] + : 216.9673, found: 216.9619

  = 0.25 (DCM/MeOH : 95/5) 1 H NMR (300 MHz, CDCl3) δ 7.26 (d, J = 8.8 Hz, 2H), 6.92 (d, J = 8.8 Hz, 2H), 6.68 (s, 1H), 6.30 (s, 1H), 3.92 (s, 3H), 3.84 (s, 3H), 3.71 (s, 3H), 3.53 (s, 1H).

13 C

 13 NMR (75 MHz, CDCl3) δ (ppm): 160.7 (Cq), 149.4 (Cq), 148.4 (Cq), 131.5 (CH), 127.8 (Cq), 124.3 (Cq), 122.4 (Cq), 114.6 (CH), 110.5 (CH), 59.0 (CH), 56.1 (2*CH3), 55.4 (CH3), 39.5 (CH2), 25.0 (CH2).

Rf = 0

 0 .34 (DCM: MeOH; 95:5) 1 H NMR (300 MHz, CDCl3) δ 7.26 (d, J = 8.8 Hz, 2H), 6.92 (d, J = 8.8 Hz, 2H), 6.68 (s, 1H), 6.30 (s, 1H), 3.92 (s, 3H), 3.84 (s, 3H), 3.71 (s, 3H), 3.53 (s, 1H). 13 C NMR (75 MHz, CDCl3) δ (ppm): 159.0 (Cq), 148.8 (Cq), 147.5 (Cq), 133.3 (CH), 131.1 (CH), 130.1 (CH), 128.4 (CH), 127.1 (Cq), 123.4 (Cq), 122.9 (Cq), 114.4 (CH), 111.2 (CH), 109.8 (CH), 55.9 (CH), 55.8 (CH3), 55.6 (CH3), 55.3 (CH3), 40.0 (CH2), 39.1 (CH2), 25.1 (CH2).

Rf = 0

 0 .38 (EtOAc 100%) IR vmax (cm -1 ) = 2921, 1702, 1515, 1442, 1211 1 H NMR (300 MHz, CDCl3) δ (ppm): 7.16 (d, J = 8.6 Hz, 4H), 6.80 (d, J = 8.6 Hz, 4H), 3.59 (s, 4H).

13 C

 13 NMR (76 MHz, CDCl3) δ (ppm): 157.8 (CO), 137.2 (s), 130.4 (s), 126.88 (s), 124.5 (s), 121.4 (CH), 121.0 (CH), 119.9 (CH), 119.3 (CH), 116.9 (CH), 116.0 (CH2), 113.2 (CH), 55.9 (CH3), 40.0 (CH2).

Rf = 0

 0 .26 (Cy/ EtOAc; 85/15) 1 H NMR (300 MHz, CDCl3) δ (ppm): 8.00 -7.92 (m, 2H), 6.99 (d, J = 8.0 Hz, 1H), 6.94 -6.86 (m, 2H), 6.86 -6.76 (m, 2H), 6.00 (ddt, J = 17.0, 10.2, 6.7 Hz, 1H), 5.19 -5.09 (m, 2H), 3.87 (d, J = 4.5 Hz, 3H), 3.77 (s, 3H), 3.41 (d, J = 6.7 Hz, 2H).

13 C

 13 NMR (101 MHz, CDCl3) δ (ppm): 138.4 (CO), 137.2 (CH2), 131.7 (Cq), 123.9 (Cq), 122.3 (CH), 121.1 (CH), 120.2 (CH), 116.4 (CH2), 113.4 (CH), 55.0 (CH3), 52.1 (CH3), 40.2 (CH2), 29.8 (s).

13 C 13 C

 1313 NMR (75 MHz, CDCl3) δ 155.9 (CO), 149.0 (OCH3), 147.6 (Cq), 131.5 (Cq), 120.7 (CH), 112.0 (CH), 111.4 (CH), 55.9 (CH3), 41.9 (CH2), 35.8 (CH2), 28.4 (CH3). HRMS m/z calcd for C15H23O4N [ESI+Na] + : 304.15149, found: 304.15193 6,7-dimethoxyphenyltetrahydroisoquinoline (321) NMR (75 MHz, CDCl3) δ (ppm): 166.6 (CO), 148.3 (Cq), 139.7 (Cq), 139.1 (Cq), 138.1 (Cq), 129.4 (CH), 128.4 (Cq), 126.3 (CH), 124.4 (CH), 118.8 (CH), 114.0 (Cq), 103.4 (CH), 52.2 (CH3). HRMS m/z calcd for C21H15O5 [ESI] + : 347.09187, found: 347.09250 7-methoxy-2,2-diphenyl-1,3-benzodioxole-5-acetate (272)

  Rf = 0.46 (Cy/EtOAc : 90/10) IR vmax (cm -1 ) = , 1718, 1634, 1435, 1331, 1124 1 H NMR (300 MHz, CDCl3) δ (ppm): 7.61 -7.56 (m, 4H), 7.37 (dd, J = 5.1, 1.8 Hz, 6H), 7.32 (d, J = 1.4 Hz, 1H), 7.28 (d, J = 1.4 Hz, 1H), 3.95 (s, 3H), 3.87 (s, 3H).

13 C

 13 NMR (75 MHz, CDCl3) δ (ppm): 166.6 (CO), 148.3 (COH), 143.3 (Cq), 139.8 (Cq), 139.7 (Cq), 139.3 (Cq), 129.4 (2*CH), 128.4 (4*CH), 126.3 (4*CH), 124.4 (Cq), 118.7 (Cq), 110.0 (CH), 104.1 (CH), 56.7 (CH3), 52.3 (CH3). HRMS m/z calcd for C22H18O5 [ESI] + : 385.10428, found: 385.10464 7-methoxy-2,2-diphenyl-1,3-benzodioxole-5-alcohol (273)

Rf = 0

 0 .26 (Cy/EtOAc : 85/15) IR vmax (cm -1 ) = 3367, 2939, 1635, 1450, 1323, 1212 1 H NMR (300 MHz, CDCl3) δ (ppm): 7.63 -7.56 (m, 4H), 7.39 -7.33 (m, 6H), 6.61 (d, J = 1.4 Hz, 1H), 6.55 (d, J = 1.3 Hz, 1H), 4.56 (d, J = 4.9 Hz, 2H), 3.93 (s, 3H).

13 C

 13 NMR (75 MHz, CDCl3) δ (ppm): 148.5 (Cq), 143.6 (Cq), 140.1 (Cq), 135.7 (2*Cq), 134.5 (Cq), 129.1 (2*CH), 128.2 (4*CH), 126.4 (4*CH), 117.4 (Cq), 106.6 (CH), 101.4 (CH), 65.5 (CH2), 56.6 (CH3). HRMS m/z calcd for C21H18O4 [ESI] + : 357.10931, found: 357.10973 7-methoxy-2,2-diphenyl-1,3-benzodioxole-5-aldehyde (274)

13 C

 13 NMR (76 MHz, CDCl3) δ 190.2 (CHO), 139.3 (Cq), 131.7 (Cq), 129.4 (2*Cq), 128.3 (4*Cq), 126.3 (4*Cq), 110.2 (CH), 103.8 (CH), 56.6 (CH3). HRMS m/z calcd for C21H17O4 [ESI] + : 356.09743, found: 356.10079 7-methoxy-2,2-diphenyl-1,3-benzodioxole-5-nitrostyrene (275)

Rf = 0. 49 (

 49 Cy/DCM : 80/20) 1 H NMR (300 MHz, CDCl3) δ 7.89 (d, J = 13.5 Hz, 1H), 7.59 -7.55 (m, 4H), 7.46 (d, J = 13.5 Hz, 1H), 7.39 (dd, J = 4.1, 2.5 Hz, 6H), 6.81 (d, J = 1.5 Hz, 1H), 6.72 (d, J = 1.5 Hz, 1H), 3.96 (s, 3H). 13 C NMR (75 MHz, CDCl3) δ (ppm): 149.3 (Cq), 144.0 (Cq), 139.3 (2*Cq), 135.6 (Cq), 129.5 (2*Cq), 128.4 (4*CH), 126.3 (4*CH), 124.4 (Cq), 118.8 (CH), 111.0 (CH), 102.4 (CH), 56.9 (CH3).

Rf = 0

 0 .56 (DCM/MeOH : 95/5) 1 H NMR (300 MHz, CDCl3) δ (ppm): 7.61 -7.58 (m, 4H), 7.36 (dd, J = 5.1, 2.0 Hz, 6H), 6.42 (d, J = 1.3 Hz, 1H), 6.33 (s, 1H), 3.91 (s, 3H), 3.31 (d, J = 6.4 Hz, 2H), 2.68 (t, J = 6.9 Hz, 2H), 1.42 (s, 9H). HRMS m/z calcd for C27H30O5N [ESI] + : 448.21182, found: 448.21185 7-methoxy-2,2-diphenyl-1,3-benzodioxole-5-amide (284) Mixture of amine 276 (0.51 g, 1.47 mmol) and acid 172 (0.22 g, 1.47 mmol) was heated neat for 2 hours at 190°C. The reaction was purified by chromatography over silica gel column, yielded a brown sticky gum (0.44 g, 62%). Rf = 0.37 (DCM/ MeOH; 95/5) 1 H NMR (300 MHz, CDCl3) δ (ppm): 7.61 (dd, J = 7.5, 2.2 Hz, 4H), 7.40 -7.36 (m, 6H), 6.92 (d, J = 8.4 Hz, 2H), 6.58 (d, J = 8.5 Hz, 2H), 6.23 (dd, J = 20.4, 1.4 Hz, 2H), 3.87 (s, 3H), 3.40 (dd, J = 12.0, 5.7 Hz, 4H), 2.62 (t, J = 6.7 Hz, 2H).

13 C

 13 NMR (75 MHz, CDCl3) δ = 171.9 (CO), 155.2 (CO), 148.8 (Cq), 143.7 (Cq), 140.6 (Cq), 133.1 (CO), 130.9 (CH), 129.4 (CH), 128.5 (CH), 126.6 (CH), 116.1 (CH), 108.2 (CH), 102.8 (CH), 56.9 (CH3), 43.1 (CH2), 40.8 (CH2), 35.6 (CH2), 3,4-dichloro-3'-bromo-4'-methoxyphenylethylamide (286) Mixture of dichlorophenethylamine 237 (0.38 g, 2.23 mmol) and acid 287 0.55 g, 2.23 mmol) was heated neat for 2 hours at 190°C. The reaction was purified by chromatography over silica gel column, yielded a brown solid (0.76 g, 82%). Rf = 0.35 (Cy: EtOAc; 90:10) 1 H NMR (300 MHz, CDCl3) δ (ppm): 7.37 (d, J = 2.0 Hz, 1H), 7.32 (d, J = 8.2 Hz, 1H), 7.18 (d, J = 2.0 Hz, 1H), 7.06 (dd, J = 8.4, 2.1 Hz, 1H), 6.89 (dd, J = 7.8, 1.6 Hz, 1H), 6.84 (d, J = 8.4 Hz, 1H), 3.90 (s, 3H), 3.45 (p, J = 6.7 Hz, 4H), 2.72 (t, J = 6.8 Hz, 2H).

13 C

 13 NMR (76 MHz, CDCl3) δ = 170.7 (CO), 155.3 (CO), 138.9 (Cq), 134.3 (CH), 132.5 (Cq), 130.7 (CH), 130.5 (CH), 129.6 (CH), 128.9 (CH), 128.0 (Cq), 112.3 (CH), 112.2, 56.3 (CH3), 42.4 (CH2), 40.4 (CH2), 34.6 (CH2).

13 C

 13 NMR (75 MHz, CDCl3) δ (ppm): 158.3 (CO), 130.4 (Cq), 123.0 (CH), 114.1 (CH), 63.9 (CH2), 55.3 (CH3), 38.3 (CH2).

Table 1 :

 1 Examples of true (or not) alkaloid skeletons.

22 Z. Řeháček and P. Sajdl. Ergot Alkaloids: Chemistry, bio effects,biotech. 1990, pp.383. 23 S. D. Sarker and L. Nahar. Organic and Nat. Prod. Chem. John Wiley & Sons Ltd, 2007, 289 -292.

Table 2 :

 2 The Shamma-Moniot classification: ethereal head-to-head links b. Aryl-aryl link

Table 3 :

 3 The Shamma-Moniot classification: aryl-aryl links c. Head-to-tail connection

Table 4 :

 4 The Shamma-Moniot classification: head-to-tail links d. Other original subclass Other original BBIQ were isolated, and molecules classed in type XXV are interesting examples. The connection between the two isoquinolines forms a keto acetal. Giannis et al. isolated new BBIQs with head-to-tail connections, without known subgroup, and moreover they described a really new type of BBIQ with on the same molecule head-to-tail connections and an aryl-aryl link 42 (compound 64 in Figure

	Figure 11 : structure of repanduline
	Newly, in 2015, A.

  [START_REF]North part" of Trilobine[END_REF]

86

F. Faltis, Justus Liebig Ann., 1932, 301-302.

[START_REF]Reaction catalyzed by palladium[END_REF]

Y. Inubushi, K-I. Nomura, Tetrahedron Lett. 1962, 24(3), 1133 -1136.

[START_REF]Reaction catalyzed by copper[END_REF]

G. Zhao, Y. Hui, J.K. Rupprecht, J.L. McLaughlin, K.V. Wood, J. Nat. Prod. 1992, 55(3), 347 -356.

[START_REF]North part" of Trilobine[END_REF]

C. Lavaud, G. Massiot, Phytochemistry 2016, 131, 7 -8.

  were able to report the first total synthesis of the optically active alkaloids: tetrandrine, isotetrandrine 85 and phaeanthine 84.

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Table 5 :

 5 optimization condition of green synthesis

	Entry	Base	Solvent	Time	Temp (°C) Yield (%)
	1.	K2CO3	DMSO	48 h	120	21
	2.	K2CO3	DMF	48 h	120	68

Table 7 :

 7 Conditions for amide reaction

	Entry	Conditions	Time Temp. Yield
	1.	HBTU (2eq)/ Et3N (3eq), DMF	24 h	0°C	0
	2.	Heat	2 h	180°C	89%

Table 8 :

 8 Conditions of large scale methylation of 4-hydroxyphenylacetic acid.

	Entry Reactant Base	Solvent Time (h) Temp (°C) Yield (%)
	1 g	Me2SO4 K2CO3 Acetone 17 h	25	96
	2 g	Me2SO4 K2CO3 Acetone 17 h	25	92
	5 g	Me2CO3 K2CO3 Acetone 17 h	25	89
	10 g Me2CO3 K2CO3 Acetone 17 h	25	88
	12 g Me2CO3 K2CO3 Acetone 17 h	25	81

Table 9 :

 9 Reduction of esters 223

	Entry Reagent	Solvent Time Temp Yield
	1.	NaBH4 (4eq)	THF	17 h	65°C 42%
	2.	Dibal-H (2.5eq)	THF	17 h	-78°C 96%

Table 10 :

 10 Oxidation of alcohol 288

	Entry Reagent	Solvent	Time Temp Yield
	1.	NaOAc (2eq)/ PCC (2eq) DCM	4 h	65°C 44%
	2.	IBX (1.5eq)	DMSO	3 h	25°C 64%

Table 11 :

 11 Direct mono-chlorination of 227

	Entry Reagent	Solvent Time Temp Yield
	1.	Oxone (1.1eq)/ NaCl Acetone 6 h	25°C 0
	2.	SO2Cl2 (1.1eq)	AcOH	3 h	25°C 94%

  H NMR (300 MHz, CDCl3) δ (ppm): 7.47 (d, J = 2.2 Hz, 1H), 7.19 (dd, J = 8.4, 2.2 Hz, 1H), 6.85 (d, J = 8.4 Hz, 1H), 3.88 (s, 3H), 3.70 (s, 3H), 3.54 (s, 2H).

	13 C NMR (75 MHz, CDCl3) δ (ppm): 171.8 (CO), 155.1 (Cq), 134.1 (CH), 129.3 (Cq), 127.5 (CH),
	111.9 (Cq), 111.6 (CH), 56.3 (CH3), 52.1 (CH3), 39.8 (CH2).
	HRMS m/z calcd for C10H11BrO3 [ESI] -: 257.98920, found: 257.98916

1

  SOCl2 (2.0 ml, 22.91 mmol) was slowly added to a mixture of acid 240 (5.01 g, 19.08 mmol) in MeOH (40 ml) and the mixture was stirred for 3 hours at 90°C. The reaction mixture was monitored by TLC (Cy/EtOAc: 8/2) on complete conversion of the starting material, and solvants were evaporated, A solution of NaHCO3 was added to neutralised and then compound was extracted with EtOAc. The organic phase was washed with brine, dried over Na2SO4 and concentrated under reduced pressure. Purification by chromatography over silica gel column yielded to an orange oil (5.21 g, 98%).

	Rf = 0.7 (Cy/EtOAc; 90/10)
	IR vmax (cm -1 ) = 3024.20, 1737.24, 1628.03, 1435.05, 1253.54, 485.41
	1 H NMR (300 MHz, CDCl3) δ (ppm): 7.67 (d, J = 8.3 Hz, 2H), 7.05 (d, J = 8.3 Hz, 2H), 3.71 (s, 3H),
	3.59 (s, 2H).
	13 C NMR (76 MHz, CDCl3) δ (ppm): 171.4 (CO), 137.7 (2*CH), 133.6 (Cq), 131.3 (2*CH), 92.6
	(Cq), 52.2 (CH3), 40.6 (CH2).
	HRMS m/z calcd for C9H9O2I: [ESI] + : 298.95382, found: 298.95394
	1 H NMR (300 MHz, CDCl3) δ (ppm): 9.73 (t, J = 2.2 Hz, 1H), 7.41 (d, J = 2.2 Hz, 1H), 7.12 (dd, J =
	8.4, 2.2 Hz, 1H), 6.89 (d, J = 8.4 Hz, 1H), 3.90 (s, 3H), 3.62 (d, J = 2.2 Hz, 2H).
	HRMS m/z calcd for C10H9BrO2 [ESI] -: 227.97861, found: 227.97859
	4-iodophenyl acetate (243)

  H NMR (300 MHz, CDCl3) δ 7.63 (d, J = 2.2 Hz, 1H), 7.15 (dd, J = 8.4, 2.2 Hz, 1H), 6.76 (d, J = 8.4 Hz, 1H), 4.57 (t, J = 7.3 Hz, 2H), 3.86 (s, 3H), 3.23 (t, J = 7.3 Hz, 2H).

1

  H NMR (300 MHz, CDCl3) δ (ppm): 7.41 (d, J = 8.2 Hz, 1H), 7.34 (d, J = 2.0 Hz, 1H), 7.08 (dd, J = 8.2, 2.1 Hz, 1H), 3.00 (t, J = 6.8 Hz, 2H), 2.75 (t, J = 6.8 Hz, 2H).

	13 C NMR (75 MHz, CDCl3) δ (ppm): 143.9 (Cq), 137.8 (Cq), 137.7 (Cq), 131.9 (CH), 131.0 (CH),
	121.2 (CH), 39.9 (CH2). 25.4 (CH2).

1

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H NMR (300 MHz, CDCl3) δ 7.41 (d, J = 8.2 Hz, 1H), 7.33 (d, J =

2.0 Hz, 1H), 7.07 (dd, J = 8.2, 2.0 Hz, 1H), 3.39 (dd, J = 13.4, 6.7 Hz, 2H), 2.80 (t, J = 7.0 Hz, 2H), 1.48 (s, 9H).

H NMR (300 MHz, DMSO-d6) δ (ppm): 7.21 (d, J = 8.4 Hz,

2H), 7.00 (d, J = 8.4 Hz, 2H), 5.96 (ddt, J = 16.9, 10.1, 6.8 Hz, 1H), 5.11 -5.04 (m, 2H), 3.37 (d, J = 6.7 Hz, 2H), 1.29 (s, 9H). HRMS m/z calcd for C16H16NO3 [M]: 205.09715, found: 205.09692.

My very special gratitude goes to the Alex Ekwueme-Federal University Ndufu-Alike Ikwo, Ebonyi State, Nigeria TETFUND) for financial support towards this research, which helped to advance my knowledge and career. Also to Campus France, your efforts to ensure that my stay to study and live in France

Scheme 4 : Synthetic route of cyclization by Inubushi et al

The compound 110 was resolved via its N-acetyl-L-leucine salt and the absolute configuration and optical purity of the resolved free base were converted into (S)-8-bromo-armepavine 111 (16% yield, Scheme 5), to be compared to the compound of the bromation of natural armepavine.

Scheme 5 : Synthesis of bromoarmepavine by Inubushi et al

The compound 111 was protected with benzoyl group to give the compound 112 which enabled the condensation by Ullmann reaction on N-t-butoxycarbonyl-3-methoxy-4hydroxyphenylethylamine 113 since the Boc group is unstable under Ullmann reaction to give diphenyl ether 114 in 48% yield (Scheme 6).

sarmentosus Diels a member of Menispermaceae. Tomita et al. 111 proposed by degradation reactions the structure of menisarine 189 (Figure 27). In this cyclization, the methoxyl bearing benzene ring was preferred rather than the analogy with the amide used for the synthesis of N-methyldihydromenisarine whereas the cyclization of the other benzene ring took place as it is observed under drastic conditions to afford dihydroisoquinoline 216 in 10% yield, such that its relative flexibility depends on the difference on their comparison. The compound 216 without purification, was reduced in the presence of Adams catalyst to yield a diastereoisomer and on methylation under Eschweiler-Clarke conditions (formaldehyde and formic acid) formed a diastereoisomeric mixture of racemic O-methyltiliacorine 217 in 16% yield (Scheme 36).

B. Previous work

As shown before, there are no very efficient conditions for Ullman-type coupling and some gave very low yields of diaryl ether. So, we looked for other methods to prepare such ethereal compounds, in particular, the aromatic nucleophilic substitution with fluoroarene or metallo catalyzed reaction (other than Ullmann-type coupling).

Nucleophilic Aromatic Substitution

The sequence 125 starts using 3-fluoro-4-hydroxy-phenyl acetic acid 220, which is protected as methyl ester and methyl ether in one step and dimethylsulfate with potassium carbonate in acetone. The resulting ester 221 can be hydrolyzed by hydrochloric acid to afford free acid 222 Scheme 49 : Retrosynthesis analysis of diiodophenylethylamine relying on 4-iodophenyl acetic acid Therefore, the 4-iodophenyl acetic acid 240 was used as a starting material and submitted to Kraszkiewicz procedure 135 to afford the corresponding 3,4-diiodophenyl acetic acid 241. Unfortunately, the reaction was over iodinated and the attempt to separate these two molecules became impossible to accomplish. Several conditions were tried as shown on Scheme 50 and Table 6, but none of them gave selectively diiodinated compound 242. The same condition reaction was tried on protected dopamine with boc 258 using a building block of 4-methoxyphenyl acetaldehyde 263. The reaction worked and 7,12-dimethylcoclaurine 264 was obtained in 39% yield. Although the yield was low when compared to reactions of Scheme 60. Maybe the acetaldehyde is more reactive or less stable because benzyl positions under these reaction condition than the benzaldehyde (Scheme 61). The preparation of natural substance dimethylcoclaurine 264 validate this sequence, but to continue the synthesis toward trilobine, we need to change protecting group since it is not possible to performe selective demethylation of benzyl methoxy group without reaction on the dimethoxy system on isoquinoline moiety.

Scheme 50 : Direct diiodination

b. Gallic acid approach

The synthesis of trioxygenated phenethylamine began by protection reaction of the cheap commercialy available gallic acid 269. The Fischer esterification was performed to form methyl gallate 270 in 83% yield. The methyl gallate 270 react with diphenyldichloromethane according to Takaoka et al. 142 and it gave the promising bisprotected 271 in 91% yield (Scheme 64).

Scheme 64 : Protection of methyl gallate Subsequently, compound 271 was methylated using M. Sani et al. 144 method and the reaction mixture worked very well to form compound 272 in 96% yield. Then, the compound 272 was reduced with Dibal-H to afford the corresponding alcohol 273 in 79% yield (Scheme 65).

Scheme 65 : Reduction of gallate

Alcohol 273 was oxidized with IBX in DMSO to obtain the aldehyde 274 in 69% yield. We then transformed the 274 by Henry's reaction according to our previously used experimental procedure with MeNO2 in the presence of NH4OAc in acetic acid. On completion of the reaction, the desired nitrostyrene compound 275 was isolated in 74% yield (Scheme 66).

Scheme 66 : Formation of nitrostyrene 275

We tried to reduce the nitrostyrene compound 275 with Dibal-H, according to Leth-Petersen et al. 138 method to form the corresponding amine 276 in 90% yield. Although, the crude product still had some residues of by-products from the reaction mixture and the attempt to possibly isolate it in pure form as the free amine became hard due to the compound 276 easily decomposed on silica gel column chromatography. To avoid this, we protected directly the crude product 276 as boc derivative, which is much easier to purify. The compound 277 was obtained in 49% yield, for these two steps (Scheme 67).

Scheme 67 : Synthesis and Protection phenylethylamine 277

With the compound 277 in hand, the Pictet-Spengler reaction with 4-methoxyphenyl acetaldehyde 263 was also tried on the same condition but in a different time interval which is aim to form a cyclized benzyltetrahydroisoquinoline compound. Unfortunately, the reaction mixture didn't work out again because the multiple products was difficult to isolate (Scheme 68). Scheme 88 : Reduction reaction So, we tried to oxidize the alcohol compound 294 with IBX in DMSO. Unfortunately, we couldn't properly isolate the aldehyde compound 295 from column chromatography (Scheme 89). Scheme 89 : Oxidation reaction Therefore, we tried to engage the diaryl ether compound 293 to saponification under the basic condition and the reaction condition was carried out in the same manner with our previous experiment on saponification and it gave us the acid compound 296 in 72% yield (Scheme 90).

Scheme 90 : Hydrolysis of diaryl ether e. Using eugenol as a model Therefore, our approach started with the commercial available eugenol 229 (as a model) and 4-iodobenzoate 297 or 4-bromobenzoate 298 in the presence of tetrakis(acetonitrile)copper (I) hexafluorophosphate and caesium carbonate as the base in xylene to give the desired diaryl ether 299 compounds in 65% yield. It is worth noticing that the tetrakis(acetonitrile)copper (I) hexafluorophosphate used is very essential for the efficiency and yield of this reaction. On changing the halide substrates to bromine, the yield reduced to 39% so we understand that the iodide gives a better yield of Ullmann coupling with a copper salt. When we changed our solvent to DMSO, we had a lower yield of 21%.

After that, we need to study the oxidative cleavage, with ozonolysis or with OsO4/NaIO4.

Transformation of allyl into aldehyde

Depending on the results of the precedent study concerning tetrahydroisoquinoline preparation, we need to establish discrimination of the two functions as aldehyde and/or acid for Pictet-Spengler and/or Bischler-Napieralski respectively. The importance is to be able to perform the two cyclization reactions sequentially to avoid the formation of mixture which is always more complicated to purify. So the preparation of this will give access to the normal and analogue aspect of trilobine compound.

EXPERIMENTAL PART

A. General experimental: Chemicals and solvents were purchased from Sigma Aldrich, Alfa Aesar, Strem Chemicals or TCI and used as received without further purification. Proton nuclear magnetic resonance ( 1 H NMR) spectra were acquired using Bruker Advance III 300 MHz or 400 MHz spectrometers. Chemical shifts (δ) are reported in parts per million (ppm) and are calibrated to the residual solvent peak 159 . Coupling constants (J) are reported in Hz. Multiplicities are reported using the following abbreviations: s = singlet; d = doublet; t = triplet; q = quartet; m = multiplet (range of multiplet is given). Carbon nuclear magnetic resonance ( 13 C NMR) spectra were acquired using Bruker Ascend 75MHz or 101 MHz spectrometers. Chemical shifts (δ) are reported in parts per million (ppm) and are calibrated to the residual solvent peak. High resolution mass spectra (HRMS) were recorded using a Bruker maXis Impact TOF mass spectrometer by electrospray ionization time of flight reflectron experiments. Fourier transform infrared (FT-IR) spectra were recorded on a Thermo Scientific Nicolet 6700 FT-IR spectrometer. Analytical thin layer chromatography was performed on pre-coated 250 mm layer thickness silica gel 60 F254 plates (EMD Chemicals Inc). Visualization was performed by ultraviolet light and/or by staining with potassium permanganate or p-anisaldehyde. Purifications by column chromatography were performed using standard column chromatography using silica gel (40-63 μm, 230-400 mesh).