teaching and research institutions in France or abroad, or from public or private research centers.


Vincent Péton

Damien Bouchard

Sintia Almeida Da Silva

Julien Jardin

Caroline Le Maréchal

Anderson Miyoshi

Vasco Azevedo

Nadejda Berkova

Lucie Rault

Patrice François

Vincent Peton

Sintia Almeida

Nadia Berkova

Jacques Schrenzel

David Hernandez

Sergine Even

Yves Le Loir
email: yves.leloir@rennes.inra.fr

Molecular and phenotypic comparison of two staphylococcus aureus strains associated to

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Introduction

Staphylococcus aureus is an opportunistic pathogen in humans and warm-blooded animals. In milk production, it is notably a major cause of mastitis in dairy ruminants, which induces huge economical loss. In this work, two S. aureus strains isolated from bovine mastitis were compared: RF122 and Newbould 305, which reproducibly induce severe and mild mastitis, respectively. The goal of this work was to deeper identify staphylococcal factors that could explain the differences of clinical signs and mastitis acuteness observed for these strains. We thus further compared the two strains, in silico and in vitro, at the genome, proteome and phenotype levels.

Genomic features

Fig. 1

 1 Fig. 1 Synteny (gene order conservation), in pairwise comparisons among two strains of S. aureus. X axis: Coordinates of S. aureus RF122 and Y axis: Coordinates of S. aureus Newbould 305.

Fig. 5 Fig. 3

 53 Fig. 5 FnbA in S. aureus 8325.4 (SWISS-Prot P14738) contains 11 Fn-binding sites. FnbA from S. aureus Newbould 305 (A) and RF122 (B) showing the approximate position of the predicted FnbRs. High-affinity FnbRs are shaded. Also shown signal peptide (S), proline-rich repeats (PRR), cell wall-spanning sequence (W), membrane-spanning region (M)

Acknowledgments : this work was financially supported by the French National Research Agency (ANR-08-ALIA-11) and Brazilian CAPES-COFECUB.

Genome organization is highly conserved between the two strains Genetic variations in these strains including SAPIs and phages, might have implications in their bovine mastitis virulence.

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