Panagiotis Kourtesis

Rayaan Amir

Josie Linnell

Ferran Argelaguet

Sarah E Macpherson

• R Amir

J Linell

Cybersickness, Cognition, & Motor Skills: The Effects of Music, Gender, and Gaming Experience

Keywords: Cybersickness, virtual reality, mitigation, cognition, reaction time, reading, eye-tracking, gender, gaming experience

Examples of Accelerations in VR: Linear(Left) and Angular(Centre

INTRODUCTION

Virtual Reality (VR) has been implemented in many areas such as education [START_REF] Radianti | A systematic review of immersive virtual reality applications for higher education: Design elements, lessons learned, and research agenda[END_REF], professional training [START_REF] Xie | A review on virtual reality skill training applications[END_REF], cognitive assessment [START_REF] Kourtesis | How immersive virtual reality methods may meet the criteria of the national academy of neuropsychology and american academy of clinical neuropsychology: A software review of the virtual reality everyday assessment lab (vr-eal)[END_REF], mental health therapy [START_REF] Emmelkamp | Virtual reality therapy in mental health[END_REF], and entertainment [START_REF] Cruz-Neira | Virtual reality and games[END_REF]. However, a principal drawback of VR is the presence of cybersickness that affects a percentage of the users [START_REF] Rebenitsch | Estimating cybersickness from virtual reality applications[END_REF]. Cybersickness pertains to nausea, disorientation, and oculomotor symptoms. While cybersickness has similarities with simulator sickness, it differs to simulator sickness in terms of severity, with accounts reporting increased general discomfort due to nauseaand disorientation-related symptoms [START_REF] Stanney | Cybersickness is not simulator sickness[END_REF]. Furthermore, cybersickness differs from motion sickness as cybersickness is triggered by visual stimulation and not by actual movement [START_REF] Davis | A systematic review of cybersickness[END_REF].

While there is not a comprehensive theory of cybersickness, the predominant one is the sensory conflict theory, which postulates that cybersickness symptomatology is induced by a conflict between our vestibular (inner ear) and visual system [START_REF] Laviola | A discussion of cybersickness in virtual environments[END_REF][START_REF] Rebenitsch | Estimating cybersickness from virtual reality applications[END_REF]. In these terms, while motion is perceived visually, the inner ear (body) does not perceive motion, which results in a conflict between proprioception and vision.

As such, the vection, an illusory sense of motion, that occurs in VR is the predominant reason for experiencing cybersickness [START_REF] Kim | Effects of linear visualvestibular conflict on presence, perceived scene stability and cybersickness in the oculus go and oculus quest[END_REF][START_REF] Nesbitt | Correlating reaction time and nausea measures with traditional measures of cybersickness[END_REF]. In VR, linear and angular accelerations may occur (see Figure 1) that induce cybersickness in the user. Cybersickness may affect the cognitive and/or the motor performance of the user. However, there are discrepant results in the relevant literature. For example, Dahlman et al. [START_REF] Dahlman | Performance and autonomic responses during motion sickness[END_REF] found that cybersickness significantly decreases verbal working memory ability. In contrast, Mittelstaedt et al. [START_REF] Mittelstaedt | Vr aftereffect and the relation of cybersickness and cognitive performance[END_REF] found no significant decrease in visual working memory. Nonetheless, several studies have found a substantial decrease in reaction times [START_REF] Mittelstaedt | Vr aftereffect and the relation of cybersickness and cognitive performance[END_REF][START_REF] Nalivaiko | Cybersickness provoked by head-mounted display affects cutaneous vascular tone, heart rate and reaction time[END_REF][START_REF] Nesbitt | Correlating reaction time and nausea measures with traditional measures of cybersickness[END_REF]. Thus, while there are inconclusive results regarding cybersickness's effects on cognition, there is agreement regarding its effect on psychomotor skills.

Furthermore, individual differences may play a role in how cybersickness is experienced. For example, the gender of the user has been suggested as a factor that could influence the intensity of cybersickness [START_REF] Stanney | Virtual reality is sexist: But it does not have to be[END_REF][START_REF] Stanney | What to expect from immersive virtual environment exposure: Influences of gender, body mass index, and past experience[END_REF]. The reasons were attributed to differences in hormones and interpupillary distance (IPD) between females and males [START_REF] Stanney | Virtual reality is sexist: But it does not have to be[END_REF][START_REF] Stanney | What to expect from immersive virtual environment exposure: Influences of gender, body mass index, and past experience[END_REF]. However, there are mixed reports in the literature, where some studies report higher cybersickness in female participants, and others an absence of significant differences between males and females [START_REF] Saredakis | Factors associated with virtual reality sickness in headmounted displays: A systematic review and meta-analysis[END_REF]. Also, other factors such as VR, computing, and/or gaming experience may modulate the user's resilience or susceptibility to cybersickness [START_REF] Saredakis | Factors associated with virtual reality sickness in headmounted displays: A systematic review and meta-analysis[END_REF]. Yet, the relationship between these aspects and cybersickness have not been adequately examined [START_REF] Saredakis | Factors associated with virtual reality sickness in headmounted displays: A systematic review and meta-analysis[END_REF].

Towards the mitigation of cybersickness symptoms, several approaches have been used, albeit that each has their limitations. For instance, "adaptation" (i.e., exposing an individual repeatedly to the cybersickness stimuli assists with building a tolerance to it) may be an efficient way to minimize cybersickness [5]. However, this process is time-consuming, costly, and requires considerable commitment. Moreover, anti-sickness drugs and supplements (e.g., ginger) have been suggested [START_REF] Golding | Pathophysiology and treatment of motion sickness[END_REF][START_REF] Wood | Comparison of efficacy of ginger with various antimotion sickness drugs[END_REF]. Yet, these are invasive approaches, and they may also cause other side effects (e.g., drowsiness) or allergic reactions [START_REF] Golding | Pathophysiology and treatment of motion sickness[END_REF]. In HCI, several techniques have been proposed such as the viewpoint control [START_REF] Farmani | Evaluating discrete viewpoint control to reduce cybersickness in virtual reality[END_REF], dynamic blurring [START_REF] Nie | Analysis on mitigation of visually induced motion sickness by applying dynamical blurring on a user's retina[END_REF], postural stability [43], and taking short breaks [START_REF] Szpak | Beyond feeling sick: The visual and cognitive aftereffects of virtual reality[END_REF]. Nonetheless, these approaches prevent certain interactions with the virtual environment (e.g., viewpoint control and postural stability) and/or may negatively affect the user experience (e.g., blurring and short breaks).

Alternatively, in medical settings, calming music has already shown promising results in mitigating nausea-related symptomatology [22]. Similar results have been found in mitigating motion sickness, where calming music was found to substantially decrease the intensity of motion sickness [START_REF] Sang | Behavioral Methods of Alleviating Motion Sickness: Effectiveness of Controlled Breathing and a Music Audiotape[END_REF]. Furthermore, pleasant music was able to alleviate motion sickness during the simulation of a bike ride [START_REF] Keshavarz | Pleasant music as a countermeasure against visually induced motion sickness[END_REF]. In a similar study, the liked music was also found to alleviate motion sickness during the simulation of a bike ride [START_REF] Peck | Examining potential effects of arousal, valence, and likability of music on visually induced motion sickness[END_REF]. These promising results indicate that music may offer relaxation (e.g., an effect against stress) or a distraction (e.g., favourite or pleasant music prevents the fixation on stimuli that induce or worsen motion sickness), which mitigate the symptomatology of motion sickness. However, as far as we are aware, no study has yet examined the effects of music on cybersickness, especially in immersive VR.

This paper reports two studies, where the 1st study (N = 92) selects music tracks that are universally rated as calming or joyful, and the 2nd study (N = 39) offers an extensive examination of cybersickness in VR. Specifically, the mitigating effects of calming and pleasant music on cybersickness are assessed. In addition, the effects of cybersickness on cognition and motor skills, as well as reading ability and pupil size, are evaluated. Finally, the role of demographics such as gender and VR, computing, and gaming experience are examined. The contributions of this paper can hence be summarized as follows:

• Providing evidence that pleasant and calming music mitigate nausea-related symptoms in VR, and pleasant music substantially alleviates overall cybersickness symptomatology.

• Highlighting the effects of cybersickness effects on cognition, motor skills, reading ability, and pupil size.

• Providing evidence that users with higher gaming experience are more resilient to cybersickness, while users with lower gaming experience are more susceptible to it.

• Examining gender differences in terms of cybersickness by evaluating and considering the important role of gaming experience on cybersickness.

RELEVANT WORK

Effect of Music on Motion Sickness

Music has been previously been implemented to alleviate motion sickness. In medical applications, there is strong evidence that relaxing music decreases nausea-related symptomatology (e.g., due to chemotherapy) [22]. In a non-medical setting, Sang et al. [START_REF] Sang | Behavioral Methods of Alleviating Motion Sickness: Effectiveness of Controlled Breathing and a Music Audiotape[END_REF] were among the first to demonstrate that music may mitigate motion sickness. Participants (N = 24) were placed in a simulator designed to trigger motion sickness, through body rotations and head movements, for 30 minutes.

The Simulator Sickness Questionnaire (SSQ) was used to evaluate sickness levels before and after the simulation. While this study provides insight into the benefits of music on motion sickness, many questions remain unanswered. Firstly, the music was played after motion sickness symptoms appeared rather than during the simulation, possibly altering the results. Also, a single music track was used, without providing a clear rationale for this selection, and they did not consider different types of music and their possible influence on motion sickness. Finally, the study was on motion sickness (i.e., body movements), but cybersickness was not studied (i.e., visually induced). Similarly, Keshavarz and Hecht [START_REF] Keshavarz | Pleasant music as a countermeasure against visually induced motion sickness[END_REF] investigated the role of pleasant music on motion sickness. Participants (N = 93) were shown a firstperson video (14 mins) of a bicycle ride with shaky movements on a large screen. Participants were randomly assigned to four groups: 1) relaxing; 2) neutral music; 3) stressful music; or 4) no music. During the video, the Fast Motion Sickness Scale (FMS) was used to evaluate motion sickness. The SSQ was also administered before and after the video. Participants also rated the pleasantness of the music. Regardless of the condition, music rated as pleasant significantly lowered motion sickness levels, whereas music rated as unpleasant did not. While these are promising results, the study had a between subjects design. Given that motion sickness varies significantly across individuals [START_REF] Golding | Pathophysiology and treatment of motion sickness[END_REF]43], a within design would have been more appropriate. Notably, the study was not conducted in immersive VR, where vection is substantially stronger, and consequently so are the visually induced symptoms [START_REF] Dennison | Use of physiological signals to predict cybersickness[END_REF].

In a follow-up study, Peck et al. [START_REF] Peck | Examining potential effects of arousal, valence, and likability of music on visually induced motion sickness[END_REF] examined how valence, arousal, and the liking of music affect motion sickness. Participants (N = 80) were allocated to one of four groups, where music of different valence and arousal was presented (i.e., happy, peaceful, agitated, and sad). Participants evaluated valence and arousal, and opted for their favourite music. Using the same video as above (see [START_REF] Keshavarz | Pleasant music as a countermeasure against visually induced motion sickness[END_REF]), four groups (N = 20 for each) were formed, where the first was presented with their favourite music, the second had high valence music, the third had high arousal music, and the control group had no music. The results showed an effect only for the highly liked music. However, this study has the same limitations as Keshavarz and Hecht, where a within design would be more efficient for controlling interindividuality in motion sickness, and immersive VR would offer more intense vection and symptomatology.

Effects of Cybersickness on Cognitive and Motor Skills

Cybersickness has been found to substantially decrease the cognitive and motor performance of the user in immersive VR [START_REF] Saredakis | Factors associated with virtual reality sickness in headmounted displays: A systematic review and meta-analysis[END_REF]. Nevertheless, the reports are not consistent. In favour of temporary cognitive decline, the study of Dahlman et al. [START_REF] Dahlman | Performance and autonomic responses during motion sickness[END_REF] postulated that motion sickness significantly decreases the verbal working memory of users. In this study (N = 38), participants were seated on a rotating optokinetic drum. Motion sickness and verbal working memory were examined before and after the session. The participants who experienced motion sickness had severely impaired verbal working memory in the post-session assessment. In contrast, the participants with low motion sickness did not show any decrease in verbal working memory. While the study supports cognitive decline due to motion sickness, the experiment is strongly related to simulator studies (i.e., rotating drum), rather than the visually induced symptomatology observed in immersive VR.

In immersive VR, Varmaghani et al. [START_REF] Varmaghani | Spatial and attentional aftereffects of virtual reality and relations to cybersickness[END_REF] conducted a study (N = 47) where the participants were allocated to two groups, a VR group (N = 25) or a control group (N = 22; playing a board game). Cybersickness and cognition were assessed before and after the respective session. The Corsi blocks task (visuospatial memory), Manikin spatial task (visuospatial processing), and Color Trails test (attentional processing and shifting) were administered. A priming effect was observed in the Manikin and Color Trails test. The outcomes showed that the VR group did not increase its visuospatial processing ability, while the control group did. This result indicated an effect of cybersickness on visuospatial processing or learning ability. However, in the remaining measures, no differences were observed. Also, the cybersickness scores did not correlate cognitive performance. Therefore, this study provided mixed results. However, they did not consider verbal memory as in [START_REF] Dahlman | Performance and autonomic responses during motion sickness[END_REF]. In addition, while cybersickness varies across individuals, this study had a between subjects design which does not permit consideration of random effects due to individual differences. Also, while an absence of correlations was reported, correlations do not provide information regarding the causal or predictive ability of a variable (e.g., cybersickness) towards another variable (e.g., cognition) [START_REF] Freedman | From association to causation via regression[END_REF]. To examine predictions and/or causation, a regression analysis (models) would have been required [START_REF] Freedman | From association to causation via regression[END_REF].

In another study, Mittelstaedt et al. [START_REF] Mittelstaedt | Vr aftereffect and the relation of cybersickness and cognitive performance[END_REF] examined cybersickness and cognition in pre-and post-sessions in VR. The participants (N = 80) were allocated into one of four groups (N = 20 for each), where different input devices or displays were used in the three groups (i.e., Gamepad-HMD, Bike-HMD, Bike-Screen), and a control group (i.e., resting in between). The SSQ (cybersicknes), the Deary-Liewald Reaction Time task (reaction time), the Mental Rotation task (spatial processing), the Corsi Block task (visuospatial working memory), the Arrow task (reaction time), and the Visual Search task (visual attention processing) were used. The reaction times of the VR groups were poorer on both corresponding tasks. Also, the VR groups did not show the improvement of the control group on the attentional processing task. On spatial processing and visuospatial working memory, there were not significant differences among groups. Overall, these results suggest a negative effect on attentional processing and reaction times, while spatial abilities and visuospatial memory remain intact. While the results support an effect of cybersickness on cognition, the outcomes were not robust. Similar to the aforementioned studies, this study had a between subjects design that does not allow for consideration of individual differences.

Furthermore, the studies of Nalivaiko et al. [START_REF] Nalivaiko | Cybersickness provoked by head-mounted display affects cutaneous vascular tone, heart rate and reaction time[END_REF] (N = 26) and Nesbitt et al. [START_REF] Nesbitt | Correlating reaction time and nausea measures with traditional measures of cybersickness[END_REF] (N = 24) examined the effects of cybersickness on reaction times. The cybersickness and reaction times were evaluated before and after exposure. The SSQ (cybersickness) and the Deary-Liewald Reaction Time task were used. The VR session consisted of a roller coaster ride, where substantial linear and angular accelerations took place to induce cybersickness in participants. In both studies, the reaction times were significantly decelerated in the post-exposure assessments. Furthermore, both studies reported significant correlations between the slowing of reaction times and the increased intensity of nausea-related symptoms. Taking together the results of [START_REF] Mittelstaedt | Vr aftereffect and the relation of cybersickness and cognitive performance[END_REF][START_REF] Nalivaiko | Cybersickness provoked by head-mounted display affects cutaneous vascular tone, heart rate and reaction time[END_REF][START_REF] Nesbitt | Correlating reaction time and nausea measures with traditional measures of cybersickness[END_REF], there is substantial evidence that reaction times are negatively affected by cybersickness.

However, none of these studies measured cybersickness during VR exposure. Furthermore, none of the studies evaluated the reaction times and cognition during the VR session (i.e., during immersion) which raises questions such as whether there is an effect only after exposure or also during exposure. Finally, while the study of Mittelstaedt et al. [START_REF] Mittelstaedt | Vr aftereffect and the relation of cybersickness and cognitive performance[END_REF] attempted mixed regression models to examine the effects of cybersickness on cognition and reaction times, the studies of Nalivaiko et al. [START_REF] Nalivaiko | Cybersickness provoked by head-mounted display affects cutaneous vascular tone, heart rate and reaction time[END_REF] (N = 26) and Nesbitt et al. [START_REF] Nesbitt | Correlating reaction time and nausea measures with traditional measures of cybersickness[END_REF] only explored correlations between cybersickness and reaction times. Thus, further investigation is required to appraise the effects of cybersickness on reaction times and cognition.

Gender and VR/Computing/Gaming Experience

Individual differences appear to play a significant role on how and when cybersickness is experienced [START_REF] Saredakis | Factors associated with virtual reality sickness in headmounted displays: A systematic review and meta-analysis[END_REF]. The most common aspect that has been examined is the gender of the user, where female users experience substantially more severe cybersickness than male users. Yet, the results are inconsistent across studies. For instance, Petri et al. [START_REF] Petri | Effects of age, gender, familiarity with the content, and exposure time on cybersickness in immersive head-mounted display based virtual reality[END_REF] conducted a study (N = 30; 15 females and 15 males) to explore gender differences. In a seated position, the participants had to observe a karate demonstration while immersed in VR. While no significant differences in objective metrics (i.e., heart rates and skin conductance) was observed between female and male participants, the subjective metrics (SSQ) revealed a significant difference between female and male participants. In contrast, the study of Melo et al. [START_REF] Melo | Presence and cybersickness in immersive content: Effects of content type, exposure time and gender[END_REF] showed no significant difference between female and male participants. However, in this study, cybersickness intensity was not high, since there were no accelerations in the virtual environment.

In contrast, in the study of Stanney et al. [START_REF] Stanney | Virtual reality is sexist: But it does not have to be[END_REF], participants (N = 46) were allocated into two groups: a VR environment (N = 30; 15 male/15 female) or a flat computer screen (N =. 16; 8 male/8 female). Cybersickness was measured before and after exposure. Gender differences were detected only in the flat screen condition. In the 2nd experiment (N = 120), the participants were allocated into eight groups (N = 15 per group) defined by gender (male vs. female), IPD (fit vs. non-fit), and motion sickness history (low vs. high). In this experiment, gender was not a significant predictor of cybersickness. Instead, only the IPD fit, motion sickness history, exposure duration, and EGG bradygastria were significant predictors. However, there were a great number of variables in the model (12 variables), which may have masked the effects of other variables on cybersickness, such as gender, gaming experience, VR exposure, hormonological cycle, and anxiety.

Furthermore, a meta-analysis of studies on cybersickness revealed no significant differences between male and female users [START_REF] Saredakis | Factors associated with virtual reality sickness in headmounted displays: A systematic review and meta-analysis[END_REF]. The meta-analysis considered only studies that were conducted in VR, and where the SSQ was administered for evaluating cybersickness. While this outcome indicates an absence of differences, the included studies were specific to cybersickness, hence, the cybersickness levels were adequately varied between them. Nevertheless, the authors also speculated that other factors should be considered such as VR and gaming experience. However, the past VR studies on cybersickness did not consider these aspects [START_REF] Saredakis | Factors associated with virtual reality sickness in headmounted displays: A systematic review and meta-analysis[END_REF].

To our knowledge, only three cybersickness studies exist that examine computing, VR, or gaming experience. The Stanney et al. [START_REF] Stanney | Virtual reality is sexist: But it does not have to be[END_REF] study considered gaming experience but did not find an effect of gaming experience on cybersickness. However, the measurement of gaming experience in this study is unclear. Traditionally, gaming (or any IT) experience is calculated by considering both the ability (i.e., how well the user operates something) and the frequency (i.e., how often the user operates something) [START_REF] Kourtesis | Validation of the virtual reality neuroscience questionnaire: Maximum duration of immersive virtual reality sessions without the presence of pertinent adverse symptomatology[END_REF][START_REF] Smith | Measuring the effect of gaming experience on virtual environment navigation tasks[END_REF]. In Stanney et al. [START_REF] Stanney | Virtual reality is sexist: But it does not have to be[END_REF], a description evaluating gaming experience is not explicitly provided, indicating that the authors did not focus on this variable.

Moreover, the study of Kourtesis et al. [START_REF] Kourtesis | Validation of the virtual reality neuroscience questionnaire: Maximum duration of immersive virtual reality sessions without the presence of pertinent adverse symptomatology[END_REF] reported no effect of gaming or VR experience on cybersickness. However, this study only reported very low intensities of cybersickness since commercial VR games were used. On the other hand, the study of Weech et al. [START_REF] Weech | Narrative and gaming experience interact to affect presence and cybersickness in virtual reality[END_REF] explored the effect of gaming experience on cybersickness. In this large study (N = 153), participants were allocated into two groups: 1) an exploration with a narrative, and 2) an exploration without a narrative. The results indicated that the narrative was effective in mitigating cybersickness, however, this was effective only when the users had higher gaming experience. Overall, this study suggested that gaming experience has an (direct or indirect) effect on cybersickness.

Summary of Literature Review

Music Effect on Cybersickness: Based on the literature review discussed above, only the effects of music on motion sickness have been examined. There is hence a gap in the literature regarding the effects of music on cybersickness in immersive VR, where vection and symptoms are stronger. Also, the studies on motion sickness and music did not have a within subjects design, which prevented them from considering variability (random effects) across individuals. Finally, only pleasant (high valence) music has been used in previous studies, while calming music has shown promising results in clinical settings.

Cybersickness's Effects on Cognitive and Motor Skills:

The literature review showed an agreement regarding the effects of cybersickness on reaction times (i.e., psychomotor skills). Visuospatial ability and visuospatial working memory appear not to be affected by cybersickness. However, while verbal working was found to be affected by motion sickness, there is not a VR study investigating cybersickness's effect on it. Verbal working memory is crucial for encoding new memories/information and facilitating the fine functioning of high order cognitive abilities like episodic memory, language, and learning [START_REF] Cowan | Working memory underpins cognitive development, learning, and education[END_REF]. Given that there are VR applications in professional training, education, and therapies, the examination of cybersickness's effects on verbal working memory is essential. Finally, studies have methodological shortcomings. They did not assess cybersickness and cognition during VR exposure/immersion, and the effects were mainly examined via correlations instead of mixed regression models.

Gender and VR/Computing/Gaming Experience: The literature review revealed contradictory outcomes regarding gender differences in terms of cybersickness. While a meticulous study indicated a gender effect on cybersickness (mainly due to the IPD), the metaanalysis showed no significant differences. The role of gender has to be further examined by considering other factors that may modulate cybersickness. Finally, the role of gaming, VR, and/or computing experience is under-investigated, while the there is a suggestion that gaming may have an effect on cybersickness.

Conclusions:

In conclusion, the current study will offer the first examination of music as a mitigating technique for cybersickness in VR. In addition, this study will be the first to study the effect of cybersickness on verbal working memory. Similarly, this study will also be the first to assess gender differences in terms of cybersickness, while it also considers the effects of VR, computing, and gaming experience on cybersickness. Finally, the current study will be the first to examine cognition and cybersickness in during VR exposure/immersion, while considering for variability across individuals, by developing and implementing a 3D-VR version of a cybersickness questionnaire.

ONLINE STUDY: EVALUATION OF CALMING AND JOYFUL MUSIC TRACKS

An online questionnaire study was conducted to obtain ratings and rankings of joyful and calming music tracks. The rating and rankings assisted with selecting two music tracks most perceived as joyful or calming. The identified best tracks were used in our main VR study. A total of 92 participants (51 males, 39 females, 2 non-binary) took part in the online questionnaire study. Participants were approached via social media. Approval for this online questionnaire study was granted by the School of Philosophy, Psychology, and Language Sciences (PPLS) Research Ethics Committee of the University of Edinburgh. All participants provided their online consent before beginning the survey. A total of twelve pre-selected instrumental tracks (each lasting approximately 30 seconds) were used. Music tracks were selected based on previous literature or were similar to those used in prior studies (e.g., [START_REF] Graff | Music versus midazolam during preoperative nerve block placements: a prospective randomized controlled study[END_REF][START_REF] Melo | Presence and cybersickness in immersive content: Effects of content type, exposure time and gender[END_REF][START_REF] Stanglmeier | Automated driving: A biomechanical approach for sleeping positions[END_REF][START_REF] Tso | Music-assisted training for dart throwing novices: Post-training effects on heart rate and performance accuracy[END_REF]).

Firstly, participants rated the first category of tracks (i.e., calming), which consisted of six calming tracks. A 7-point Likert scale, where 1 indicated not at all calming and 7 indicated extremely calming, was presented to participants, asking them to rate how calming the track was. Participants were instructed to listen to the whole 30 second clip before submitting a response, and were unable to pause the clip or proceed onto the next track prior to the clip's completion. Following the individual ratings, a list of each of the tracks (30 seconds each) was presented to each participant. The participants were instructed to listen to each clip once more and rate these in comparison to one another. Participants were asked to drag and drop (rank) the same six tracks in order of preference. Following this, the same procedure was repeated for the six joyful tracks, with the rating of individual tracks followed by an overall comparative ranking.

The joyful track included the the Sock Shopping by Ray Amir (Joyful A), the Brandenburg Concerto No.4 in G Major, Allegro by Bach (Joyful B), the Good Times (instrumental version) by Chic (Joyful C), the Thunder and Lightning polka by Johann Strauss (Joyful D), the Spring by Vivaldi (Joyful E), and the Summertime by Morning Light Music (Joyful F). The calming tracks included the Watermark by Enya (Calming A), the Piano Bar Jazz by Pinegroove Music (Calming B), the Mellow Sky by Ray Amir (Calming C), the Maharishi Gandharva Veda by Amar Nath (Calming D), the Electra by Airstream (Calming E), and the Weightless by Marconi Union (Calming F). The 30 seconds excerpts of the music tracks can be downloaded from the Open Science Framework (OSF) repository through this LINK

Results

Analyses were conducted in R [40]. The final track selection was determined by considering both rating and ranking. Wilcoxon signedrank tests were performed to compare tracks within categories, and the p-values were corrected for multiple comparisons.

Joyful Tracks: The Wilcoxon signed-rank test showed tracks B, C, and E to have significantly higher ratings than the other tracks (p < .05). However, there was no significant difference among these tracks. The comparisons of rankings of these tracks showed that track C had a significantly better ranking than B (p < .05), while E did not differ substantially. According to the frequencies of the tracks' ratings, 82.6% of the respondents rated Joyful C with a 4 (i.e., joyful) or higher. Notably, 59.8% of respondents gave track C a rating of 5 (very joyful) or greater (i.e., very much joyful or extremely joyful), the highest of all tracks. Therefore, Joyful track C (i.e., the instrumental version of Good Times by Chic) was selected for the VR experiment. The two best joyful tracks can be downloaded from the OSF repository through this LINK Calming Tracks: The Wilcoxon signed-rank test showed tracks C and F to have significantly higher ratings than the other tracks (p < .05). However, the comparison of rankings indicated that track C has a significantly better ranking than the others (p < .05). Additionally, according to the frequencies of the tracks' ratings, 87.7% of the respondents rated Calming C with a 4 (i.e., calming) or higher. Importantly, 64.1% of the respondents gave track C a rating of 5 (i.e., very calming) or greater (i.e., very much calming or extremely calming), the highest of all tracks. Thus, Calming track C (i.e., the Mellow Sky by Ray Amir) was chosen for the VR experiment. The two best calming tracks can be downloaded from the OSF repository through this LINK

IMMERSIVE VR STUDY

The main experiment was conducted to examine the effects of cybersickness on cognition (i.e., verbal working memory and visuospatial working memory) and motor skills (i.e., reaction time). Equally, this experiment strove to examine the effects of music, gender, and IT experience (VR, gaming, and computing experience) on cybersickness.

Virtual Environment Design and Interactions

The Unity3D game engine was used for the development of the virtual environment. The SteamVR SDK was used for designing the interactions with it. Since gaming experience may modulate task performance [START_REF] Kourtesis | How immersive virtual reality methods may meet the criteria of the national academy of neuropsychology and american academy of clinical neuropsychology: A software review of the virtual reality everyday assessment lab (vr-eal)[END_REF], the virtual hands/gloves of SteamVR SDK were used for ensuring an ergonomic and effortless interaction. Importantly, the interactions did not require any button to be pressed, everything was facilitated by touching the object (initial selection) and a continuous touching of the object (a confirmation of the selection). Also, the SteamVR virtual gloves did not connote any gender or race, which assisted with preventing confounding effects from these variables [START_REF] Schwind | These Are Not My Hands![END_REF]. Furthermore, Amazon Polly was used to produce audio clips with neutral naturalistic voices, to provide the instructions to the users. Note that users received instructions in video, audio, and written form, to ensure their understanding and seamless completion of the tasks. The audio (especially for feedback sounds) was spatialized using the Stea-mAudio plugin. The SRapinal SDK was used for eye-tracking and facilitating the pupillometry, as well as the fixation duration over the text of the cybersickness questionnaire (i.e., reading ability). Finally, the randomization of the experimental blocks within and between participants, extraction of the data into a CSV file, and the experimental design and control were attained using bmlTUX SDK [START_REF] Bebko | bmltux: Design and control of experiments in virtual reality and beyond[END_REF].

Rides with Linear and Angular Accelerations/

As the literature suggests, linear and angular accelerations are able to induce significant cybersickness symptoms to the users in a relatively short time (e.g., ∼ 5 -10 mins) [START_REF] Kourtesis | Validation of the virtual reality neuroscience questionnaire: Maximum duration of immersive virtual reality sessions without the presence of pertinent adverse symptomatology[END_REF][START_REF] Nalivaiko | Cybersickness provoked by head-mounted display affects cutaneous vascular tone, heart rate and reaction time[END_REF][START_REF] Nesbitt | Correlating reaction time and nausea measures with traditional measures of cybersickness[END_REF]43,[START_REF] Saredakis | Factors associated with virtual reality sickness in headmounted displays: A systematic review and meta-analysis[END_REF]. In accordance, we designed the ride to last 5 mins, since it had to be repeated three times (i.e., total 15 min ride) for each participant, and it had to last comparably with the duration of the music tracks (∼ 5 mins per track). The ride was designed as an animation of the platform that the user was standing on (see Figure 1). The overall direction of the motion was always forward (except the last stage; see reversed z axis). The movements were similar to those of a roller coaster. The ride consisted of the following accelerations (in this specific order): a) linear (z axis), angular (z and y axes), angular (z, x, and y axes), angular (roll axis), extreme linear (z axis), angular (yaw axis), and extreme linear (y axis followed by reversed z axis). The environment was a simple black and white surrounding (see Figure 1) to ensure that the symptoms were only induced by vection, and not due to other reasons (e.g., intense colours). The squared/tiled design assisted with providing cues for eliciting vection and perceiving the altitude changes.

Cognitive and Psychomotor Skills' Assessment

Since the examination of cybersickness, cognition, and motor skills had to be repetitive, while the user is immersed in VR, immersive VR versions of well-established tasks/tests were developed. For the development of these VR cognitive and psychomotor tasks, the specific design and development guidelines and recommendations for cognitive assessments in immersive VR were followed [26].

Verbal Working Memory

A VR version of the Backward Digit Span Task (BDST; [START_REF] Wechsler | Wechsler bellevue adult intelligence scale[END_REF]) was developed and used. In the VR BDST, the participants listened to a series of digits, which they needed to remember and recall in the reverse order of their presentation. For example, if participants heard 2, 4, and 3, then they should respond by indicating the reverse order (i.e., 3, 4, 2). After listening to the digits, a keypad appeared in front of participants, which they had to use for providing the digits in the reverse order. To indicate a number, participants touched the white box button that displays the equivalent number (see Figure 2). While touching the button, it turned blue. To confirm their response, participants needed to keep touching the button for a second. Then, if the response was correct, the button turned green, and made a positive sound. If the response was incorrect, then the button turned orange, and made a negative sound. Each trial ended when participants made a mistake or when they provided the digits correctly in their reverse order. Every second successful trial, the length of the digit sequence was increased. The task was ended when they made two subsequent mistakes within the same digit sequence length (e.g., 3 digits), or when they finished the last trial (i.e., second trial with a sequence length of 7 digits). The Total Score was the addition of the number of successful trials and the highest digit sequence length that at least one trial was successfully performed.

Visuospatial Working Memory

Visuospatial working memory was assessed using the VR version of the Backward Corsi Block Test (BCBT) [6] that we developed. In this task, there are 27 white boxes, each one is placed in a different position on the x, y, and z axes. However, out of the 27 possible boxes, participants were randomly shown only nine boxes in each trial (see Figure 2). Each trial began with the presentation of 9 boxes. A number of these boxes (depending on the current sequence length) were randomly presented (turning blue and making a bell sound) in sequential order. Each box from the sequence was presented for one second. At the end of the sequence's presentation, participants had to select the boxes in the reverse order. To select a cube, participants touched it and it turned blue. After touching the cube for one second, the target was selected. Once selected, it either turned green and made a positive sound (i.e., correct response), or it turned orange and made a negative sound (i.e., an error). The trial was ended when participants either made a mistake, or they correctly selected all the targets in their reverse order. The sequence lengths were initially two boxes, with two attempts for each length. The number of boxes in the sequence was increased by one box when at least one of the two trials of the same length/span was correct. When the participant incorrectly recalled two sequences of the same length, the task was ended. Likewise, when the second trial of the last length/span (i.e., 7 cubes) was performed, then the task was ended. Sequence lengths increased up to seven which indicated a perfect score if no mistakes were made. The Total Score was calculated by summing the span (correct sequence with the most cubes) and the total number of correct sequences.

Psychomotor Skills

A VR version of the Deary-Liewald (DL) Reaction Time task [START_REF] Deary | A free, easy-to-use, computerbased simple and four-choice reaction time programme: the deary-liewald reaction time task[END_REF] was developed and used to assess reaction times. The DL Reaction Time Task (also referred to as the DL task) contained two tasks for assessing simple reaction time (SRT) and choice reaction time (CRT). During the SRT task, participants were asked to observe a white box and to touch it as soon as the box changed into blue (see Figure 2). The SRT task consisted of 20 trials/repetitions. In the CRT task, one of the four boxes (aligned horizontally) randomly changed blue. Participants had to touch the box, once the box turned blue (see Figure 2). This task consisted of 40 trials/repetitions. In both SRT and CRT, the participants were instructed to touch the boxes as fast as possible using either hand. Both the SRT and CRT had a practice session at the start to ensure that the instructions were understood by the participants.

The SRT was scored by averaging the total reaction time across the 20 trials. Likewise, the recorded times for the CRT were averaged to produce a score. However, the CRT had three scores. The eyetracking enabled us to measure the time required to attend to the target, since the time it was presented (Attentional Time). Also, this allowed us to calculate the time required to touch the target since the time that was attended (Motor Time). Finally, the overall time between the target's presentation until its selection (Reaction Time) was also measured. Thus, three scores were produced: 1) the Reaction Time (RT) to indicate the overall psychomotor speed; 2) the Attentional Time (AT) to indicate the attentional processing speed; 3) the Motor Time (MT) to indicate the movement speed.

Cybersickness in VR Questionnaire

The Cybersickness in VR Questionnaire (CSQ-VR) was used to assess cybersickness symptomatology and intensity. The CSQ-VR is derived from the VR Induced Symptoms and Effects of the VR Neuroscience Questionnaire, which has been found to have very good structural and construct validity [START_REF] Kourtesis | Validation of the virtual reality neuroscience questionnaire: Maximum duration of immersive virtual reality sessions without the presence of pertinent adverse symptomatology[END_REF]. The CSQ-VR has been validated against the SSQ and the VRSQ, and showed superior reliability to them [START_REF] Kourtesis | Cybersickness in virtual reality questionnaire (csq-vr): A validation and comparison against ssq and vrsq[END_REF]. The advantages of CSQ-VR pertain to its short administration procedure (only 6 items/questions) and the production of easily comprehensible outcomes [START_REF] Kourtesis | Cybersickness in virtual reality questionnaire (csq-vr): A validation and comparison against ssq and vrsq[END_REF][START_REF] Somrak | Suitability and comparison of questionnaires assessing virtual reality-induced symptoms and effects and user experience in virtual environments[END_REF]. Moreover, the CSQ-VR assesses all the sub-types of cybersickness symptoms, such as nausea, disorientation, and oculomotor. For each sub-type, there are 2 questions. Each question is presented on a 7-item Likert Scale, ranging from "1 -absent feeling" to "7 -extreme feeling", where each response is a combined text and number (see Figure 3). The CSQ-VR produces a Total Score and three subscores, one for each type of symptom (i.e., a Nausea Score, a Disorientation Score, and a Oculomotor Score). Each subscore is calculated by adding the two corresponding responses, and the total score by adding the three subscores. The CSQ-VR can be accessed via this LINK.

Since the aims of this study required the repeated assessment of cybersickness while the user is immersed in VR, the use a short, yet inclusive and valid, questionnaire like CSQ-VR was preferred. An User Interface (UI) was designed and developed where the question is displayed in the upper region, and the response (in red) in the middle region. Using a slider, the users could change their response, by touching the corresponding number, or sliding along the slider (see Figure 3). In addition, based on the established link between verbal working memory and reading speed [START_REF] Dixon | Word knowledge and working memory as predictors of reading skill[END_REF], as well as between pupil size and affective/emotional state [36], we integrated eye-tracking metrics to explore these aspects. Invisible eye-tracking targets were placed in front of the text, while their height and width was always matched to the displayed text per line (see Figure 3). The use of these targets allowed us to measure the Fixation Duration over the text as a metric of reading speed. Also, the measurement of pupil size was continuous while the user responded to the CSQ-VR questions. This enabled us to measure the average Pupil Size (right and left) during this process, as a physiological metric of negative emotion.

Participants and Procedures

An a priori power analysis indicated a sample size of 36 participants or greater is required for the statistical analyses. A total of 39 participants (22 females, 17 males) aged between 22 and 36 years [M(SD) = 25.28(3.25)] were recruited for the current study. Education (in years) ranged from 13 to 20 years [M(SD) = 17.23 (1.60)]. For the comparisons between male and female participants, for being matched in terms of age and education, a sample of 32 (16F and 16M) was considered. Recruitment was achieved via opportunity sampling, using the internal mailing lists of the University of Edinburgh, alongside advertisements on social media. The study was approved by the School of PPLS Ethics Committee of the University of Edinburgh. Written consent was obtained from all participants prior to their participation. Participants were compensated with £20 each for their time and effort.

The Motion Sickness Susceptibility Questionnaire (MSSQ; [17]) was completed prior to enrolment, to reduce the likelihood of severe symptoms following VR exposure. In line with the MSSQ author suggestions [17], the 75th percentile was used as a parsimonious cutoff score for inclusion in the study. This allowed us to exclude the individuals who are prone to experience strong symptoms (i.e., the upper 25th percentile of the population). The included participants were then invited to attend the experiment. Upon arrival, participants were informed of the study's aims and procedures, and the adverse effects that they may experience. The participants then provided their informed consent.

Every participant went through an induction on how to wear the headset and use and hold the controllers. An HTC Vive Pro Eye was used, which embeds an eye-tracker with a binocular gaze data output frequency of 120Hz (i.e., refresh rate), a tracking accuracy of 0.5°-1.1°, a 5-point calibration, and a 110°trackable field of view. The participants then provided their demographic data: age, sex, gender, education, dominant eye, VR experience, computing experience, and gaming experience, by responding to a questionnaire.VR/computing/gaming experience were calculated by adding scores from two questions (6-item Likert Scale) for each one. The first question was regarding the participants' ability (e.g., 5 -highly skilled) to operate a VR/computer/game, and the second one was regarding the frequency of operating them (e.g., 4 -once a week).

After the demographics, participants were immersed in VR. Note that during immersion, the participants were always (i.e., assessments and rides) in a standing position in the middle of the VR area (see X mark in Figure 1. They started with the baseline assessment, during which a video tutorial was offered, alongside verbal and written instructions, prior to each questionnaire or task. Also, since previous research indicated that the subjective perception of the music track may affect the efficiency of the music in mitigating motion sickness [START_REF] Peck | Examining potential effects of arousal, valence, and likability of music on visually induced motion sickness[END_REF], we included a questionnaire (only in the baseline condition) to obtain participants' ratings. Using an UI comparable to CSQ-VR (see Figure 3), participants listened to an excerpt (30 secs) of each track (Calming and Joyful), and then rated them based on a 7-point Likert scale (1 = joyful/calming; 7 = extremely joyful/calming).

The rest of the tasks were performed again after each ride: the CSQ-VR questions, the verbal working memory task (BDST), the visuospatial working memory task (BCBT), and the reaction time task (DL; see subsection 4.2). After the baseline assessment, the first ride started. In each ride (see subsubsection 4.1.1), a different type of music was offered (i.e., Joyful, Calming, or No Music), where each track lasted approximately 5 minutes (i.e., the full track), throughout the ride. The participants completed four assessments (Baseline + one assessment after each ride) and three rides. The procedure thus was Baseline Assessment-Ride1-Assessment1-Ride2-Assessment2-Ride3-Assessment3. The order of the types of music was counterbalanced across participants to avoid confounding order effects. The whole procedure in VR lasted approximately 100 mins for each participant. After the VR session, refreshments rich in electrolytes were offered. Moreover, the participants rested for 10-15 mins before leaving the premises. They were instructed to avoid driving and using heavy machinery.

Hypotheses and Exploratory Aims

Based on our literature review (see subsection 2.4), we formulated the following hypotheses:

• [H1] The Calming music will have a significant mitigating effect on the intensity of the nausea-related symptoms.

• [H2] The Joyful music will have a significant mitigating effect on overall cybersickness.

• [H3] Cybersickness will substantially decrease verbal working memory abilities and reaction times.

• [H4] Gaming, VR, and/or computing experience will have a mitigating effect on cybersickness.

• [H5] Female participants will experience more intense cybersickness symptoms compared to male participants. In addition to the hypotheses, based on the links between verbal working memory and reading, as well as between (positive/negative) emotions and pupil size, the current study explored the effects of cybersickness on reading ability and pupil size. Finally, this study examined the reasons for the contradictory reports in the literature on gender differences in terms of cybersickness, by examining other demographic data and their relationship or effect on cybersickness.

RESULTS

All analyses were performed using R [40]. As the variables violated the normality assumption, we used the bestNormalize R package [START_REF] Peterson | Ordered quantile normalization: a semiparametric transformation built for the cross-validation era[END_REF] to transform and centralize the data. The distribution of the data was then normal. Furthermore, the a f ex (ANOVA analyses; [47]), the ggplot2 (plots; [START_REF] Wickham | ggplot2: Elegant Graphics for Data Analysis[END_REF]), the emmeans (post-hoc comparisons; [30]), and the lme4 (regression analyses; [START_REF] Bates | Fitting linear mixedmodels using lme4[END_REF]) R packages were used for performing all the analyses. Two-way repeated measures (RM) ANOVA analyses were conducted to examine the existence of interactions between the order of ride and the type of music, as well as their main effects on cybersickness. Mixed regression model analyses were performed to investigate the effect of cybersickness on cognition, motor skills, reading ability, and pupil size. Mixed model analyses were also performed to explore the effects of demographics (age, gender, education, and VR/computing/gaming experience) on cybersickness. T-tests were performed for examining differences between female and male participants. Finally, an ANCOVA was conducted, where gaming experience was a covariate, to examine whether females and males indeed experience substantially different levels of cybersickness. For the RM ANOVA, the Greenhouse Geisser's correction was applied when the sphericity assumption was violated. The Bonferroni correction was applied to correct for multiple comparisons in the post hoc tests.

Order, Music, and Cybersickness

The two-way ANOVAs revealed no significant interactions between music type and ride order on cybersickness. However, ride order revealed significant main effects on Nausea [F(2.45, 92.92) = 7.68, p < .001, ω 2 = .11], Vestibular [F(2.8, 106.48) = 7.11, p < .001, ω 2 = .10], and Oculomotor symptoms [F(2.26, 85.74) = 8.77, p < .001, ω 2 = .12], as well as overall cybersickness [F(2.76, 105) = 11.91, p < .001, ω 2 = .15]. Although the rides differ significantly from the baseline (see Figure 4), the post hoc comparisons did not reveal significant differences among the rides for overall cybersickness, nausea, or vestibular scores. Yet, significant differences between 1st vs 2nd ride, and 1st vs 3rd ride, were observed for Oculomotor symptoms (see Figure 4). However, these differences may be due to the fatigue that is under the oculomotor category of symptoms.

In support of H1 & H2, significant main effects of music were detected for Nausea [F(2.35, 89.32) = 12.32, p < .001, ω 2 = .17], Vestibular [F(2.52, 95.88) = 8.27, p < .001, ω 2 = .11], and Oculomotor symptoms [F(2.42, 92.03) = 5.56, p < .001, ω 2 = .07], as well as overall cybersickness [F(2.53, 95.97) = 14.51, p < .001, ω 2 = .17]. However, the post hoc comparisons revealed that, except for the significant difference against the baseline, there were no other significant difference amongst the music types for vestibular and oculomotor symptoms. In contrast, regarding the Nausea symptoms, there were significant differences between Calming Music vs No Music, and Joyful Music vs No Music (see Figure 4). These results are aligned with H1 & H2, since mitigating effects (substantially lower intensity of symptoms) of Calming and Joyful music are detected. Equally, in support of H2, there was a significant difference between Joyful Music vs No Music (see Figure 4), where Joyful music substantially decreased the intensity of overall cybersickness. Finally, the ratings of joyful and calming music tracks by the participants did not show any significant correlation with cybersickness.

Cybersickness on Cognition, Motor Skills, Reading Ability, and Pupil Size

Cybersickness had an effect on verbal working memory, albeit there was no effect on visuospatial working memory. In line with H3, overall cybersickness had a negative effect on verbal working memory (see Table 1 and Figure 5). While all types of cybersickness symptoms had an effect on verbal working memory, the nausea-and vestibularrelated symptoms had a slightly higher negative effect (see t value and β coefficient in Table 1). Furthermore, in support of H3, overall cybersickness had an effect on reaction time. As displayed in Figure 5, higher cybersickness increases the reaction time, postulating a substantial deceleration. However, only the vestibular symptoms appeared to have an effect on reaction time, while there were no significant effects on reaction time by nausea and oculomotor symptoms (see Table 1).Furthermore, cybersickness (overall and symptoms) had no effect on attention time or motor time. Interestingly, the effects on motor time were very small (see β in Table 1). In contrast, the effects on attention time tended to be significant, and their size was large (see β in Table 1). Given that reaction time is the addition of attention and motor time, the results indicate that the effects on reaction time predominantly stem from a substantial deceleration of attentional processing speed. Moreover, the exploratory analyses revealed significant effects of cybersickness on reading time and pupil size (see Figure 5). Every symptom type substantially prolonged reading time (see Table 1). Lastly, except for nausea symptoms, the other types of cybersickness symptoms substantially decreased pupil size.

Cybersickness and Individual Differences

As displayed in Table 2, gender and gaming experience were the only demographics that predicted cybersickness intensity. In line with H4, individuals with higher gaming experience had substantially lower overall cybersickness, as well as vestibular symptoms (see Figure 6). Nonetheless, gaming experience did not significantly predict nausea or oculomotor symptoms. On the other hand, the user's gender significantly predicted the intensity of cybersickness. In support of H5, female participants experienced substantially more intense cybersickness symptoms compared to male participants [t(37) = 3.08, p = .004]. However, another significant difference was identified between female and male participants. As illustrated in Figure 7, female participants had a significantly lower gaming experience than the male participants [t(37) = -2.76, p < .001]. Given that gaming experience was a significant predictor of cybersickness with a large β (see Table 2),gaming experience was considered as a covariate in the AN-COVA for assessing gender's effect on cybersickness. In contrast with H5, there was not a significant main effect of gender on cybersickness [F(1, 30) = 0.93, p = .45, ω 2 = .01e -03], postulating that the observed differences in cybersickness were attributed to the difference in gaming experience between female and male participants. Finally, post hoc comparisons of the marginal means indicated that there were not any significant differences between female and male users when gaming experience was matched between them (see Figure 7).

DISCUSSION

Music and Cybersickness

Calming music was found to have a significant mitigating effect on nausea symptoms induced by cybersickness. These results are in line with the findings in clinical populations (e.g., cancer patients), where calming/relaxing music reduced nausea induced by trauma (e.g., brain injury) or drugs (e.g., chemotherapy) [22]. However, pleasant/joyful music was also successful in substantially alleviating nausea. Notably, only the pleasant/joyful music was able to substantially mitigate the intensity of overall cybersickness. This outcome is in line with the findings of previous studies on motion sickness (e.g., [START_REF] Keshavarz | Pleasant music as a countermeasure against visually induced motion sickness[END_REF][START_REF] Peck | Examining potential effects of arousal, valence, and likability of music on visually induced motion sickness[END_REF]). Nevertheless, in contrast with the study of Peck et al. [START_REF] Peck | Examining potential effects of arousal, valence, and likability of music on visually induced motion sickness[END_REF], the ratings of joyful and/or calming music did not correlate with cybersickness, postulating that the liking of a song did not play a significant role. We infer that the reason for this disagreement is that the music tracks used in our study were derived from a relatively large scale online study. Consequently, and as observed in the ratings of the VR study, both tracks were highly rated by the participants.

The mitigation of nausea symptoms by music has been suggested to arise from a synchronization of the autonomous nervous system [3]. However, this theory fails to explain how pleasant/joyful music is even more effective in mitigating overall motion-or cybersickness. An alternative explanation is that pleasant music has a combined effect, where it distracts the user from stimuli that induce symptoms, and also elicits an arousal of positive emotions [START_REF] Peck | Examining potential effects of arousal, valence, and likability of music on visually induced motion sickness[END_REF]. Our results suggest that joyful music was more successful than calming music, since it mitigated both nausea and overall cybersickness. Also, the ratings indicated that the song was perceived as highly joyful by the vast majority of the participants.

Moreover, pupil size has been previously seen as a biomarker of affective state, where bigger size indicates a positive and smaller size indicates a negative affective state [36]. Our results demonstrated a comparable pattern in cybersickness, where more intense cybersickness induced a smaller diameter (i.e., more negative affective state), and less intense a bigger diameter of the pupils (i.e., more positive affective state). Notably, the mixed regression model of cybersickness explained 83% of the variance of pupil size. Thus, these results further support the notion that pleasant music is efficient in mitigating cybersickness due to distraction from stimuli, as well as an elicitation of positive emotions. In summary, the implementation of music appears to be a cost-effective, suitable (i.e., without preventing other interactions or breaking the immersion), and efficient technique for mitigating cybersickness in immersive VR.

Cybersickness's Effects on Cognitive and Motor Skills

In the current study, cybersickness was found to substantially affect performance on a verbal working memory task. This outcome aligns with the previous observation in motion-sickness, where verbal working memory was significantly decreased [START_REF] Dahlman | Performance and autonomic responses during motion sickness[END_REF]. However, in our study, the visuospatial working memory remained intact regardless of cybersickness. These results agree with the previous findings where visuospatial working memory was not affected by cybersickness [START_REF] Mittelstaedt | Vr aftereffect and the relation of cybersickness and cognitive performance[END_REF]. Taken to-gether, our and previous results postulate that negatively affects only the verbal working memory, while the non-verbal processes remain intact. This could indicate that cybersickness may affect also higher cognitive functions, which require an intact verbal working memory, such as episodic memory and learning (see [START_REF] Cowan | Working memory underpins cognitive development, learning, and education[END_REF] for the link between these cognitive functions).

Moreover, in our study, reading time was substantially increased during cybersickness, connoting slower reading speed. Considering the association between verbal working memory and reading ability [START_REF] Cowan | Working memory underpins cognitive development, learning, and education[END_REF], this result further suggests that the verbal processes are being affected by cybersickness. Nevertheless, regarding reading ability, this observation in our study could have stemmed from a confusion in decision making (e.g., selecting an appropriate response to the question, which motivated a re-reading of the question) rather than decreased reading speed. Thus, further research is required for understanding whether reading ability is indeed affected, or there is another cognitive process (e.g., decision making or comprehension) that is affected by cybersickness.

Furthermore, in line with previous findings [START_REF] Mittelstaedt | Vr aftereffect and the relation of cybersickness and cognitive performance[END_REF][START_REF] Nalivaiko | Cybersickness provoked by head-mounted display affects cutaneous vascular tone, heart rate and reaction time[END_REF][START_REF] Nesbitt | Correlating reaction time and nausea measures with traditional measures of cybersickness[END_REF], a substantial deceleration of reaction time was detected. However, we did not find a significant deceleration of the components of psychomotor skills. Neither attentional processing time, nor motor time was significantly affected. Nevertheless, the effect of cybersickness on attentional processing speed tended to be significant, which supports the findings of Mittelstaedt et al. [START_REF] Mittelstaedt | Vr aftereffect and the relation of cybersickness and cognitive performance[END_REF], where visual attention speed was significantly slowed by cybersickness. In our study, the effect of cybersickness on attentional speed was large, while the effect size on motor speed was small. Considering that reaction speed consists of attentional and motor speed, these results indicate that the significant decrease in reaction speed is attributed predominantly to the decrease of the attentional processing speed. These effects of cybersickness are crucial in VR applications in education and/or occupational training, where intact attention and reflexes are required.

Gender and Gaming Experience in Cybersickness

Only gender and gaming experience of the user were found to significantly predict the intensity of the experienced cybersickness symptoms. In contrast with research on motion sickness, where MSSQ is efficient in predicting the intensities of motion sickness [17], the scores of MSSQ were not efficient in predicting the intensity of cybersickness.This inability of MSSQ to predict cybersickness agrees with the fact that cybersickness substantially differs from simulator and motion sickness in terms of stimuli eliciting the symptoms (e.g., vection instead of motion) and the frequency of symptoms (e.g., disorientation is the most frequent in cybersickness) [START_REF] Davis | A systematic review of cybersickness[END_REF][START_REF] Stanney | Cybersickness is not simulator sickness[END_REF]. Furthermore, age did not appear to predict cybersickness. However, it should be considered that the participants in the current study were young adults. The effect of age should be examined in an age-diverse population. Lastly, neither VR nor computing experience predicted cybersickness. Regarding the effect of VR experience on cybersickness, a systematic review showed there are discrepant results in the literature, where some studies reported a resilience in experienced VR users, while others did not detect differences [START_REF] Tian | A review of cybersickness in headmounted displays: raising attention to individual susceptibility[END_REF]. Though, previous studies suffered from did not meticulously measuring VR experience [START_REF] Tian | A review of cybersickness in headmounted displays: raising attention to individual susceptibility[END_REF]. In this study, VR experience, as well as computing and gaming experience, were measured in a standardized and effective way [START_REF] Kourtesis | Validation of the virtual reality neuroscience questionnaire: Maximum duration of immersive virtual reality sessions without the presence of pertinent adverse symptomatology[END_REF][START_REF] Smith | Measuring the effect of gaming experience on virtual environment navigation tasks[END_REF], yet, VR experience failed to predict cybersickness. However, the population did not appear to have high levels of experience. Hence, the effect of VR experience should be further studied in a population with greater variability in terms of VR experience.

In line with the findings of [START_REF] Chattha | Motion sickness in virtual reality: An empirical evaluation[END_REF][START_REF] Stanney | Virtual reality is sexist: But it does not have to be[END_REF], we did find significant differences between male and female participants in terms of cybersickness intensity. However, Stanney et al. [START_REF] Stanney | Virtual reality is sexist: But it does not have to be[END_REF] suggested that these differences derive from the IPD of the VR headset. Indeed, in their 2nd experiment, they did not find any difference when participants found the IPD comfortable. However, in our study, we used an HTC Vive Pro Eye, which is comfortable for everyone. Also, every participant went through eye-tracking calibration, where the IPD is adjusted for the successful completion of calibration. Hence, every participant had an appropriate IPD and felt comfortable. Regardless of the IPD adjustment and comfort, differences in cybersickness intensities were present. Thus, other factors appear to modulate the differences between female and male users.

In our study, gaming experience was a significant predictor of cybersickness, which aligns with the findings of the large scale study of Weech et al. [START_REF] Weech | Narrative and gaming experience interact to affect presence and cybersickness in virtual reality[END_REF]. Also, our findings on cybersickness agree with previous studies, where higher gaming experience associated with augmented resilience to simulator sickness [START_REF] Himmels | The influence of gaming experience and optic flow on simulator sickness: Insights from a driving simulator study[END_REF] and cybersickness [START_REF] Grassini | Association of individual factors with simulator sickness and sense of presence in virtual reality mediated by head-mounted displays (hmds)[END_REF]. Moreover, the gaming experience of female participants was significantly smaller compared to male participants. Previous studies who reported higher cybersickness in females compared to males (e.g., [START_REF] Chattha | Motion sickness in virtual reality: An empirical evaluation[END_REF][START_REF] Stanney | Virtual reality is sexist: But it does not have to be[END_REF]) did not consider gaming experience for explaining these differences. In our study, when we controlled for gaming experience, the intensities of cybersickness did not significantly differ between female and male users. This result agrees with the outcome of a meta-analysis of studies on cybersickness, which suggested no difference between males and females [START_REF] Saredakis | Factors associated with virtual reality sickness in headmounted displays: A systematic review and meta-analysis[END_REF]. Importantly, it postulates that gaming experience is a factor that substantially modulates the differences between female and male VR users. Higher gaming experience indicated a resilience to cybersickness, while lower gaming experience indicates a susceptibility to it. However,it should be noted that this study considered experience as a combination of the ability (i.e., how capable someone is of using VR/PC or playing games) and the frequency (i.e., how often someone uses VR/PC or plays games) pertinent to VR, computers, and video games. Gaming experience should thus be efficiently evaluated and considered in future studies on cybersickness.

Limitations and Future Studies

The current study has several limitations that should be considered. Firstly, the population consisted of young adults. The effects of cybersickness should be examined in a more age-diverse population, where the role of individual differences and demographics could be further studied. In addition, cybersickness intensities in the current study mainly ranged between mild to moderate symptoms. This probably is a result of the parsimonious inclusion criterion that we used for the MSSQ scores (i.e., excluding individuals who scored higher than the 75th percentile who could experience substantially stronger symptoms). Since cybersickness intensities appear to substantially vary across individuals, future studies should examine the effects of music on cybersickness and cybersickness effects on cognitive and motor skills in a population that may experience stronger symptoms. Also, the VR exposure in this study lasted approximately 70 minutes (100 mins for the whole protocol, pre and post exposure) per participant, which is considered a long exposure to VR. It would be of interest to replicate the findings of the present study in a shorter exposure. Lastly, the current study used only working memory tests and a classical psychomotor task. Future studies should attempt to examine other cognitive functions (e.g., episodic memory or decision making) and motor skills (e.g., tasks that require fine motor skills and accuracy).

CONCLUSION

Music, especially joyful, appeared a cost-effective and efficient method for mitigating nausea and overall cybersickness. Also, cybersickness was found to affect only verbal working memory but not non-verbal (visuospatial) working memory. Given the important role of verbal working memory in higher cognitive functions, this effect of cybersickness should be considered in VR applications for educational, training, and clinical purposes. Moreover, Pupil size was substantially affected by cybersickness, and it can thus be considered as a biomarker of cybersickness. Higher gaming experience was associated with lower cybersickness, and explained the differences between female and male users in terms of cybersickness. Gaming experience should be appropriately evaluated and considered in future studies on cybersickness.

Fig. 2 :

 2 Fig. 2: Digit Span Task (Upper Left), Corsi Block Task (Upper Right), and Deary Liewald Reaction Time Tasks (Bottom).

Fig. 3 :

 3 Fig. 3: CSQ-VR User Interface and Eye-Tracking (ET) Targets. Note: Eye-tracking targets were not visible to the user.

Fig. 4 :

 4 Fig. 4: Overall Cybersickness (Top -Left) and Nausea (Bottom -Left) Intensities by Type of Music. Overall Cybersickness (Top -Right) and Oculomotor (Bottom -Right) Intensities by Order of Ride. *** p < .001, ** p < .01, and * p < .05

Fig. 5 :

 5 Fig. 5: Overall Cybersickness's Effect on Verbal Working Memory (Top Left), Reaction Time (Top Right), Reading Time (Bottom Left), Pupil Size (Bottom Right).

Fig. 7 :

 7 Fig. 7: Gaming Experience Difference between Female and Male Participants (Left). Cybersickness in Female and Male Participants per Gaming Experience Level (Right).

Table 1 :

 1 Mixed Regression Models for Cognition, Motor Skills, Reading Ability, and Pupil Size.

	Predicted	Predictor	F(1,156)	t(156)	β	p-value	R 2
		Nausea	1.43	1.20	0.10	= .21	.38
	Visuospatial	Vestibular	0.56	0.75	0.07	= .45	.37
	Working Memory	Oculomotor	0.95	0.97	0.08	= .33	.38
		Cybersickness	0.15	0.39	0.02	= .70	.33
		Nausea	7.41	-2.72	-0.16	<.01**	.70
	Verbal	Vestibular	4.27	-2.07	-0.16	<.05*	.69
	Working Memory	Oculomotor	5.38	-2.32	-0.14	<.05*	.70
		Cybersickness	6.98	-2.64	-0.17	<.01**	.71
		Nausea	1.03	0.68	0.04	.31	.74
	Reaction Time	Vestibular Oculomotor	7.51 0.75	1.79 0.27	0.13 0.02	<.01** = .39	.74 .74
		Cybersickness	3.84	0.97	0.09	<.05*	.74
		Nausea	2.60	1.61	0.13	= .11	.25
	Attention Time	Vestibular Oculomotor	3.10 3.05	1.76 1.75	0.18 0.15	= .06 = .06	.27 .26
		Cybersickness	2.24	1.50	0.14	= .11	.26
		Nausea	0.22	0.47	0.03	= .47	.60
	Motor Time	Vestibular Oculomotor	0.05 1.66	0.21 1.29	0.02 0.09	= .26 = .20	.60 .60
		Cybersickness	0.23	0.47	0.04	= .65	.60
		Nausea	38.63	6.22	0.43	<.001***	.49
	Reading Time	Vestibular Oculomotor	32.13 11.3	5.67 3.36	0.50 0.26	<.001*** <.001***	.44 .44
		Cybersickness	24.39	4.94	1.51	<.001***	.61
		Nausea	2.59	-1.61	-0.07	= .11	.82
	Pupil Size	Vestibular Oculomotor	4.95 10.5	-2.22 -3.24	-0.13 -0.15	<.05* <.001***	.83 .83
		Cybersickness	8.69	-2.95	-0.15	<.001***	.83

Table 2 :

 2 Mixed Regression Models for Cybersickness: Demographics.

	MSA & MSB = motion sickness susceptibility indexes
	Predictor	F(1,156) t(156) β coefficient	p-value	R 2
	Age	0.05	-0.22	-0.03	= .83	.46
	Education	0.03	0.16	0.02	= .87	.46
	Gender	9.47	-3.08	-0.65	<.001*** .46
	Computing Experience	0.25	0.50	0.07	= .62	.46
	VR Experience	1.00	-1.00	-0.12	= .32	.46
	Gaming Experience	4.00	-2.00	-0.24	<.01**	.46
	MSA -Child	2.06	1.43	0.10	= .15	.42
	MSB -Adult	0.88	0.94	0.06	= .35	.42
	Fig. 6: Gaming Experience Effect on Overall Cybersickness (Left)
	Vestibular (Right) Intensity.

ACKNOWLEDGMENTS

The authors would like to thank the uCreate Studio of the University of Edinburgh for providing them with VR equipment and tech support.