Simona Barni
email: simonabarni@hotmail.com

Davide Caimmi
email: davide.caimmi@gmail.com

Fernanda Chiera
email: fernandachiera@hotmail.it

Pasquale Comberiati
email: pasquale.comberiati@gmail.com

Carla Mastrorilli
email: carla.mastrorilli@icloud.com

Umberto Pelosi
email: umberto.pelosi@gmail.com

Francesco Paravati
email: paravati.f@gmail.com

Gian Luigi Marseglia
email: gl.marseglia@smatteo.pv.it

Stefania Arasi
email: stefania.arasi@opbg.net

Phenotypes and Endotypes of Peach Allergy: What Is New?

Keywords: peach allergy, food allergy, molecular allergy, Pru p 3, Pru p 7, peamaclein, anaphylaxis, oral allergy syndrome, pollen-food allergy syndrome, oral immunotherapy

Peach allergy is emerging as a common type of fresh-fruit allergy in Europe, especially in the Mediterranean area. The clinical manifestations of peach allergy tend to have a peculiar geographical distribution and can range from mild oral symptoms to anaphylaxis, depending on the allergic sensitization profile. The peach allergen Pru p 7, also known as peamaclein, has recently been identified as a marker of peach allergy severity and as being responsible for peculiar clinical features in areas with high exposure to cypress pollen. This review addresses the latest findings on molecular allergens for the diagnosis of peach allergy, the clinical phenotypes and endotypes of peach allergy in adults and children, and management strategies, including immunotherapy, for peach allergy.

Epidemiology

Peach (Prunus persica) is the fruit of Prunus trees, belonging to the Rosaceae family, including 4828 known species in 104 genera [START_REF] Haleema | A Review on Pharmacological Activities of Prunus persica[END_REF][START_REF] Christenhusz | The number of known plants species in the world and its annual increase[END_REF][START_REF]The Plant List: Rosaceae[END_REF]. In addition to peaches, apples, pears, quinces, apricots, plums, cherries, raspberries, loquats, strawberries, and almonds belong to the Rosaceae family [START_REF]The Families of Flowering Plants[END_REF].

Currently, the peach plant is cultivated in different parts of the world. Peach cultivation is believed to have originated in China and to have been transported, via the silk route, to India, the Middle East, and Persia, before finally spreading towards Europe. China, Italy, Spain, Turkey, and the USA are the leading peach-producing countries [START_REF] Kant | A Review on Peach (Prunus persica): An Asset of Medicinal Phytochemicals[END_REF].

Peach fruit usually ripens between August and September [START_REF] Haleema | A Review on Pharmacological Activities of Prunus persica[END_REF]. Peach can be eaten as fresh fruit as well as in treated forms, such as canned, dried, juice, and jam [START_REF] Kant | A Review on Peach (Prunus persica): An Asset of Medicinal Phytochemicals[END_REF].

Peach has been described as a common cause of fresh-fruit allergy in Europe, especially in the Mediterranean area [START_REF] Cuesta-Herranz | Peach allergy pattern: Experience in 70 patients[END_REF]. The prevalence data on fruit allergies are limited, and the available data are derived from scarce studies, especially in children [START_REF] Zuidmeer | The prevalence of plant food allergies: A systematic review[END_REF][START_REF] Burney | Prevalence and distribution of sensitization to foods in the European Community Respiratory Health Survey: A EuroPrevall analysis[END_REF][START_REF] Burney | The prevalence and distribution of food sensitization in European adults[END_REF]. In a systematic review conducted by Zuidmeer et al., the overall perceived prevalence of fruit allergies Nutrients 2022, 14, 998 2 of 14 ranged from 0.1% to 4.3% [START_REF] Zuidmeer | The prevalence of plant food allergies: A systematic review[END_REF]. In particular, 2.2-11.5% of children aged 0-6 years and 0.4-6.6% of adults are affected by fruit allergies, based on self-reported data. One Europeanbased large survey reported the highest and lowest prevalence of allergic sensitization to peach in Germany (11.7%) and Iceland (0.3%), respectively [START_REF] Burney | Prevalence and distribution of sensitization to foods in the European Community Respiratory Health Survey: A EuroPrevall analysis[END_REF]. In another, similar survey, the highest prevalence of peach sensitization was observed in Switzerland (13.4%) and the lowest in Iceland (2.3%) [START_REF] Burney | The prevalence and distribution of food sensitization in European adults[END_REF]. The overall European prevalence of allergic sensitization to peach increased from 5.4% in 2010 [START_REF] Burney | Prevalence and distribution of sensitization to foods in the European Community Respiratory Health Survey: A EuroPrevall analysis[END_REF] to 7.9% in 2014 [START_REF] Burney | The prevalence and distribution of food sensitization in European adults[END_REF]. The prevalence data on peach-allergen-specific sensitization have been investigated in Spanish and Italian studies: lipid-transfer protein (LTP) sensitization is predominant in Southern Europe, whereas sensitization to pathogenesis-related 10 (PR-10) is more common in Northern and Central Europe, including areas with Fagales pollen exposure (birch, alder, hazel, hornbeam, oak, beech, and chestnut) [START_REF] Boyano-Martinez | Peach allergy in Spanish children: Tolerance to the pulp and molecular sensitization profile[END_REF][START_REF] Pastorello | Anti-rPru p 3 IgE levels are inversely related to the age at onset of peach-induced severe symptoms reported by peach allergic adults[END_REF].

Similar to other IgE-mediated food allergies, peach allergy negatively impacts quality of life, causing stress and anxiety. Peach allergy, as with fruit allergies in general, is reported to be associated with less-severe symptoms than food allergies to peanuts and tree nuts; nevertheless, the condition exerts a similar impact on patients' quality of life: 60% of adults are impacted by fruit allergy in their daily life at home and 62% in their life outside the home [START_REF] Le | Reported food allergy to peanut, tree nuts and fruit: Comparison of clinical manifestations, prescription of medication and impact on daily life[END_REF].

Peach Allergens

To date, six peach allergens have been recognized [START_REF]WHO/IUIS Allergne Nomenclature Sub-Committee[END_REF]. Detailed information on each allergenic protein is provided in Table 1 .

Table 1. Main features of peach molecular allergens. Modified from [START_REF] Allergy | f95 Peach[END_REF].

Allergen

Biochemical Name Molecular Weight (kDa) Main Characteristic

Pru p 1

Pathogenesis-related protein group 10, (PR-10), Bet v 1 family member [START_REF] Peterson | Stable Plant Food Allergens I: Lipid Transfer Proteins[END_REF] Mainly found in areas with high birch pollen exposure [START_REF] Boyano-Martinez | Peach allergy in Spanish children: Tolerance to the pulp and molecular sensitization profile[END_REF].

Pru p 2 Thaumatin-like protein (TLP) 25-28 Pru p 2 from peach was one of the probable allergens causing fruit allergies [START_REF] Palacin | The involvement of thaumatin-like proteins in plant food cross-reactivity: A multicenter study using a specific protein microarray[END_REF].

Pru p 3

Non-specific lipid-transfer protein 1 (nsLTP1) 10

Major allergen [START_REF] Boyano-Martinez | Peach allergy in Spanish children: Tolerance to the pulp and molecular sensitization profile[END_REF]. Present in the outer surface of peach [START_REF] Tuppo | Peamaclein-A new peach allergenic protein: Similarities, differences and misleading features compared to Pru p 3[END_REF]. In total, 54 (96%) out of 57 children showed positive Pru p 3-sIgE in a Spanish study [START_REF] Boyano-Martinez | Peach allergy in Spanish children: Tolerance to the pulp and molecular sensitization profile[END_REF].

Pru p 4 Profilin 14

Minor allergen [START_REF] Boyano-Martinez | Peach allergy in Spanish children: Tolerance to the pulp and molecular sensitization profile[END_REF]. In total, 52 (12.1%) out of 430 patients were sensitized to profilins in an adult study [START_REF] González-Mancebo | Prevalence of Sensitization to Lipid Transfer Proteins and Profilins in a Population of 430 Patients in the South of Madrid[END_REF].

Pru p 7

Gibberellin-regulated protein (GRP) 6910.84 Da (Mass spectrometry)

Major allergen [START_REF] Klingebiel | Pru p 7 sensitization is a predominant cause of severe, cypress pollen-associated peach allergy[END_REF]. Identified in 2012 [START_REF]WHO/IUIS Allergne Nomenclature Sub-Committee[END_REF]. Present both in the pulp and in the peel [START_REF] Tuppo | Peamaclein-A new peach allergenic protein: Similarities, differences and misleading features compared to Pru p 3[END_REF]. Sensitization to Pru p7 was present in 171 (54%) out of 316 subjects with suspected peach allergy [START_REF] Klingebiel | Pru p 7 sensitization is a predominant cause of severe, cypress pollen-associated peach allergy[END_REF]. Pru p 7 sensitization was more frequent in peach-allergic (123/198, 62%) than in peach-tolerant (48/118, 41%) patients, p-value = 0.0002 [START_REF] Klingebiel | Pru p 7 sensitization is a predominant cause of severe, cypress pollen-associated peach allergy[END_REF].

Pru p 9 Pathogenesis-related protein PR-1 18

Identified in 2018 [START_REF]WHO/IUIS Allergne Nomenclature Sub-Committee[END_REF]. Sensitization to peach-tree pollen was rated third, after olive tree and grass [START_REF] Victorio Puche | Peach Tree Pollen and Prunus persica 9 Sensitisation and Allergy in Children and Adolescents[END_REF], in areas with peach-tree cultivars.

In total, 205 (30%) out of 685 children were sensitized to Pru p 9 on skin prick test [START_REF] Victorio Puche | Peach Tree Pollen and Prunus persica 9 Sensitisation and Allergy in Children and Adolescents[END_REF].

kDa: kilodaltons, IgE: immunoglobulin E; sIgE: specific IgE.

Pru p 1

Pru p 1 is a member of the PR-10 protein family and is present in the pulp and the skin of peach [START_REF] Gaier | Purification and structural stability of the peach allergens Pru p 1 and Pru p 3[END_REF]. It shares a structural homology with the major birch pollen, Bet v1 [START_REF] Gaier | Pru p 3 as a marker for symptom severity for patients with peach allergy in a birch pollen environment[END_REF]. For this reason, Pru p1 sensitization is commonly found in Northern and Central Europe, where the exposure to birch pollen is high and usually results in oral allergy syndrome (OAS) symptoms [START_REF] Gaier | Pru p 3 as a marker for symptom severity for patients with peach allergy in a birch pollen environment[END_REF]. Pru p1 cross-reacts with other PR-10 protein families, such as Rosaceae fruits, hazelnut, carrots, and celery [START_REF] Sastre | Molecular diagnosis in allergy[END_REF]. Pru p 1 is heat-labile and it is found to be sensitive to gastrointestinal digestion [START_REF] Gaier | Purification and structural stability of the peach allergens Pru p 1 and Pru p 3[END_REF]. Thus, only the unprocessed form of the fruit leads to the typical symptoms of OAS, whereas cooked peach is tolerated by patients [START_REF] Sastre | Molecular diagnosis in allergy[END_REF].

Pru p 3

Pru p 3 is a non-specific LTP (nsLTP) [START_REF] Carnes | Pru p 3 (LTP) content in peach extracts[END_REF]. The outer surface of the peach (pericarp) has a high concentration of nsLTP [START_REF] Peterson | Stable Plant Food Allergens I: Lipid Transfer Proteins[END_REF]. The peel contains seven times higher LTP than the pulp [START_REF] Sastre | Molecular diagnosis in allergy[END_REF]. Pru p 3 cross-reacts with the nsLTP contained in the other fruits of the Rosaceae family (apple, plum, cherry and apricot), as well as in vegetables (asparagus, lettuce, tomato, maize, onion, and carrot) and nuts (walnut, hazelnut, almond and peanut) [START_REF] Egger | The role of lipid transfer proteins in allergic diseases[END_REF]. The LTP nsLTP is a plant panallergen due to its widespread distribution among plant-foods and pollens [START_REF] Sastre | Molecular diagnosis in allergy[END_REF]. The LTPs from different plant-food and pollens can cross-react with each other, causing sensitization and, eventually, symptoms in multiple plant foods, a condition also known as "LTP syndrome" [START_REF] Pascal | Lipid transfer protein syndrome: Clinical pattern, cofactor effect and profile of molecular sensitization to plant-foods and pollens[END_REF]. Pru p 3 is resistant to heat and proteolytic digestion. Therefore, the clinical manifestations of Pru p 3 sensitization can range from mild OAS symptoms to severe systemic allergic reactions (anaphylaxis) [START_REF] Zamieskova | Natural variability of restriction profiles in non-coding part of Prunus persica (L.) Batsch. Pru p 3 gene[END_REF][START_REF] Salcedo | Plant non-specific lipid transfer proteins: An interface between plant defence and human allergy[END_REF].

Pru p 4

Pru p 4 is a profilin, which is an important pan-allergen, widely found in pollens and vegetables [START_REF] Cuesta-Herranz | Pollen allergy in peach-allergic patients: Sensitization and cross-reactivity to taxonomically unrelated pollens[END_REF]. Pru p 4 is present in the pulp and peel of peach [START_REF] Boyano-Martinez | Peach allergy in Spanish children: Tolerance to the pulp and molecular sensitization profile[END_REF]. Pru p 4 cross-reacts with profilins from other members of the Rosaceae family (i.e., apple and cherry) and with profilin from unrelated families' pollen (i.e., Artemisia vulgaris, Betula alba, Corylus avellanus, P amygdalus) [START_REF] Ando | Pru p 7 Predicts Severe Reactions after Ingestion of Peach in Japanese Children and Adolescents[END_REF]. Pru p 4 is heat-labile and it can be destroyed by gastrointestinal digestion [START_REF] Yang | Differential transcript abundance and genotypic variation of four putative allergen-encoding gene families in melting peach[END_REF]. For this reason, the usual clinical manifestation of Pru p 4 sensitization is OAS [START_REF] Ando | Pru p 7 Predicts Severe Reactions after Ingestion of Peach in Japanese Children and Adolescents[END_REF][START_REF] Pastorello | Pru p 3-sensitised Italian peach-allergic patients are less likely to develop severe symptoms when also presenting IgE antibodies to Pru p 1 and Pru p 4[END_REF].

Pru p 7

Pru p 7 is a gibberellin-regulated protein (GRP) [START_REF] Tuppo | Peamaclein-A new peach allergenic protein: Similarities, differences and misleading features compared to Pru p 3[END_REF], also known as Snakin/GASA [START_REF] Nahirnak | Snakin-1 affects reactive oxygen species and ascorbic acid levels and hormone balance in potato[END_REF], that was first described by Tuppo et al. in 2013 [27]. Pru p 7 has been found both in the pulp and the peel of the peach [START_REF] Tuppo | Peamaclein-A new peach allergenic protein: Similarities, differences and misleading features compared to Pru p 3[END_REF]. Pru p 7 cross-reacts with several fruits of the Rosaceae (i.e., apricot and pomegranate) and Rutaceae family (i.e., orange), as well as pollens from the Cupressaceae family [START_REF] Inomata | Gibberellin-regulated protein in Japanese apricot is an allergen cross-reactive to Pru p 7[END_REF][START_REF] Tuppo | Pomegranate Cultivars: Identification of the New IgE-Binding Protein Pommaclein and Analysis of Antioxidant Variability[END_REF][START_REF] Inomata | Identification of gibberellin-regulated protein as a new allergen in orange allergy[END_REF][START_REF] Charpin | Cypress Pollinosis: From Tree to Clinic[END_REF]. Indeed, Pru p 7 sensitivity seems to be most common in areas with high cypress pollen exposure [START_REF] Klingebiel | Pru p 7 sensitization is a predominant cause of severe, cypress pollen-associated peach allergy[END_REF]. Pru p 7 is resistant to heat and proteolytic digestion [START_REF] Tuppo | Peamaclein-A new peach allergenic protein: Similarities, differences and misleading features compared to Pru p 3[END_REF]. Thus, the typical allergic symptom of Pru p 7 sensitization is anaphylaxis. Similar to Pru p 3, sensitization to Pru p 7 is considered a risk factor for severe allergic reactions to fresh fruit [START_REF] Klingebiel | Pru p 7 sensitization is a predominant cause of severe, cypress pollen-associated peach allergy[END_REF]. Biagioni et al. [START_REF] Biagioni | Allergy to Gibberellin-Regulated Proteins (Peamaclein) in Children[END_REF] recently reported the first case series of children with documented Pru p 7 allergies and provided a diagnostic algorithm. The authors suggest performing skin prick tests (SPT) for inhalant and food allergens, including both cypress pollen and Pru p 3-enriched peach peel extracts, in case of a systemic allergic reaction to fruit. In cases of a positive SPT for both cypress-and Pru p 3-enriched peach peel extract and a negative in vitro result for specific IgE (sIgE) to Pru p 3, the diagnosis of Pru p 7 allergy is highly probable. In these cases, whenever possible, determining serum sIgE levels of Pru p 7 is recommended.

Pru p 9

Pru p 9 is a pathogenesis-related protein PR-1, identified in 2018 [START_REF]WHO/IUIS Allergne Nomenclature Sub-Committee[END_REF], with a molecular weight of 18 kDa. In 685 Spanish children and adolescents affected by rhino-conjunctivitis and asthma, the sensitization to peach-tree pollen was rated third, after olive tree and grass. Thirty percent (205 out of 685) of children were sensitized to Pru p 9 on skin prick testing [START_REF] Victorio Puche | Peach Tree Pollen and Prunus persica 9 Sensitisation and Allergy in Children and Adolescents[END_REF]. The rate of sensitization to Pru p 9 in children is similar to that in adults from the same area [START_REF] Somoza | Subjects develop tolerance to Pru p 3 but respiratory allergy to Pru p 9: A large study group from a peach exposed population[END_REF]. Pru p 9 is considered a new occupational allergen from peach-tree pollen involved in rhinitis and asthma [START_REF] Somoza | Subjects develop tolerance to Pru p 3 but respiratory allergy to Pru p 9: A large study group from a peach exposed population[END_REF].

Clinical Manifestations

Similar to other IgE-mediated food allergic reactions, symptoms appear within minutes to two hours from peach ingestion, except for food-dependent exercise-induced anaphylaxis, which can occur up to 4 h later. Reactions can be triggered by the allergen through the oral route, rarely by inhalation or skin contact, and may affect one or more target organs, including the oral mucosa, the skin, the gastrointestinal tract, the respiratory tract, and the cardiovascular system [START_REF] Muraro | EAACI food allergy and anaphylaxis guidelines: Diagnosis and management of food allergy[END_REF][START_REF] Asero | Peach-induced contact urticaria is associated with lipid transfer protein sensitization[END_REF][START_REF] Pérez-Calderón | Occupational respiratory allergy in peach crop workers[END_REF].

Immediate peach-induced reactions could be associated with two clinical patterns: the pollen-food allergy syndrome (PFAS) and a primary food allergy [START_REF] Klingebiel | Pru p 7 sensitization is a predominant cause of severe, cypress pollen-associated peach allergy[END_REF].

The clinical manifestations of peach allergy depend on the sensitization profile and, consequently, have a peculiar geographical distribution.

In Northern and Central Europe, peach allergy is mainly secondary to pollen allergy. In this condition, also known as PFAS, pollen allergens are the causative agents of the primary sensitization and food allergy to fruits and vegetables results from cross-reactivity between pollen and food allergens. Conversely, in Mediterranean countries, fruit allergy without related pollinosis is often observed and systemic reactions are frequently reported [START_REF] Fernandez-Rivas | Apple allergy across Europe: How allergen sensitization profiles determine the clinical expression of allergies to plant foods[END_REF][START_REF] Fernandez-Rivas | Clinically relevant peach allergy is related to peach lipid transfer protein, Pru p 3, in the Spanish population[END_REF].

While, on one hand, it is true that allergy to Pru p 1 is mainly associated with pollenfruit allergy syndrome, and that Pru p 9 allergy is associated with respiratory symptoms, on the other hand, patients allergic to either Pru p 3 and/or Pru p 7 are at risk of developing severe symptoms, including anaphylaxis and fatal anaphylaxis [START_REF] Klingebiel | Pru p 7 sensitization is a predominant cause of severe, cypress pollen-associated peach allergy[END_REF][START_REF] Lee | Targeted temperature management after cardiac arrest with anaphylaxis[END_REF][START_REF] Barradas Lopes | Allergy to lipid transfer proteins (LTP) in a pediatric population[END_REF].

Peach Allergy Secondary to Pollen Allergy

The allergen families involved in peach-induced PFAS include PR10 proteins, profilins, nsLTPs, thaumatin-like proteins, and gibberellin-regulated proteins [START_REF] Poncet | Update on pollen-food allergy syndrome[END_REF].

PFAS account for up to 60% of food allergies in adult patients and adolescents. It may affect one or more target organs: the skin, the oral mucosa, the gastrointestinal tract, the respiratory tract, and the cardiovascular system [START_REF] Poncet | Update on pollen-food allergy syndrome[END_REF][START_REF] Werfel | Position paper of the EAACI: Food allergy due to immunological cross-reactions with common inhalant allergens[END_REF].

The most frequent clinical pattern observed in adult patients and adolescents with PFAS is OAS. Symptoms emerge within 5-15 min of food ingestion and consist of tingling/itching of the lips, tongue, oral mucosa, palate, and throat, with possible mild angioedema associated at the same sites [START_REF] Werfel | Position paper of the EAACI: Food allergy due to immunological cross-reactions with common inhalant allergens[END_REF].

Most cases resolve spontaneously within 30 min, but 3% of patients present systemic reactions without oropharyngeal symptoms, and 1-8% develop systemic reactions, such as urticaria, dyspnea, wheezing, and anaphylaxis [START_REF] Ma | A survey on the management of pollen-food allergy syndrome in allergy practices[END_REF][START_REF] Kim | Clinical Manifestations and Risk Factors of Anaphylaxis in Pollen-Food Allergy Syndrome[END_REF][51].

Acute generalized urticaria, with or without angioedema, and contact urticaria are the second most frequently observed symptoms of PFAS. Gastrointestinal symptoms, such as nausea, vomiting, abdominal pain, and diarrhea are rarely seen as exclusive manifestations of PFAS. Respiratory symptoms, such as rhinoconjunctivitis, bronchospasm, and laryngeal edema occur more frequently in association with other target organs symptoms rather than in isolation [START_REF] Werfel | Position paper of the EAACI: Food allergy due to immunological cross-reactions with common inhalant allergens[END_REF].

The presence of comorbidities (atopic dermatitis) and cofactors (exercise, alcohol consumption, use of non-steroidal anti-inflammatory drugs (NSAIDs)) increases the severity of symptoms and the risk of anaphylaxis [START_REF] Kim | Clinical Manifestations and Risk Factors of Anaphylaxis in Pollen-Food Allergy Syndrome[END_REF].

Primary Peach Allergy

Primary food allergy to peach, in which the sensitization occurs through the ingestion of the food, is mainly related to nsLTP Pru p 3, although some studies reported primarily airborne sensitization to nsLTPs [START_REF] Sanchez-López | Role of Art v 3 in pollinosis of patients allergic to Pru p 3[END_REF][START_REF] Borghesan | Respiratory allergy to lipid transfer protein[END_REF].

In the Mediterranean area, there is a high rate of sensitization to nsLTPs, which represents the most frequent cause of both primary food allergy and food-dependent anaphylaxis in adults living in these countries [START_REF] Lyons | Food Allergy in Adults: Substantial Variation in Prevalence and Causative Foods Across Europe[END_REF][START_REF] Asero | Causes of food-induced anaphylaxis in Italian adults: A multi-centre study[END_REF].

The sensitization to Pru p 3 often occurs early in life. It may be isolated (monosensitization) or associated with multiple nsLTP sensitizations, which may lead to multiple plant-food allergies (nsLTP-syndrome) [START_REF] Skypala | The diagnosis and management of allergic reactions in patients sensitized to non-specific lipid transfer proteins[END_REF].

Pru p 3 sensitization may be asymptomatic or manifest with variable symptom severity, ranging from OAS to anaphylaxis [START_REF] Asero | The clinical relevance of lipid transfer protein[END_REF][START_REF] Scala | Lipid transfer protein sensitization: Reactivity profiles and clinical risk assessment in an Italian cohort[END_REF].

OAS and contact urticaria are the most frequent clinical patterns observed in LTP hypersensitivity. Gastrointestinal symptoms (nausea, vomiting, abdominal pain, diarrhea) may occur as isolated symptoms or in association with the cutaneous, respiratory, or cardiovascular symptoms involved in anaphylaxis [START_REF] Asero | The clinical relevance of lipid transfer protein[END_REF].

A study on LTP syndrome reported that in a group of 87 patients sensitized to Pru p 3, 44% had anaphylaxis, 43% presented OAS or urticaria, and 13% were asymptomatic. The culprit food belonged to the Rosaceae family in 48.8% of the subjects, and the most frequent food involved was peach in both symptomatic groups [START_REF] Basagaña | Clinical Profile of Lipid Transfer Protein Syndrome in a Mediterranean Area[END_REF].

Co-sensitization to birch pollen (Bet v 1) and/or to profilin is associated with a lower prevalence of severe reactions and a higher prevalence of local reactions (OAS) [START_REF] Scala | Lipid transfer protein sensitization: Reactivity profiles and clinical risk assessment in an Italian cohort[END_REF].

A large prospective study evaluated the phenotype and severity biomarkers of peachallergic patients sensitized to Pru p 3. The authors showed that most patients were sensitized to other LTP-containing plant foods (LTP syndrome), while only 6.8% were LTPmonoallergic (reacting only to peach and not to other plant foods). Subjects with LTP syndrome had a younger onset of peach allergy, and more asthma and sensitization to Parietaria and profilin than the LTP-monoallergic patients. Anaphylaxis was significantly more frequent in the LTP-monoallergic group, which had no sensitization to profillin. The presence of profilin sensitization was associated with a lower risk of anaphylaxis. No correlation was observed between SPT diameter, Pru p 3 sIgE level, level of nsLTP sensitization, and severity of reaction to peach [START_REF] Bogas | Phenotyping peach-allergic patients sensitized to lipid transfer protein and analysing severity biomarkers[END_REF].

Individuals with sensitization to Pru p 3 may develop cross-sensitization to other nsLTPs containing plant foods due to the structural homology between different nsLTPs. Pru p 3 shows a sequence homology from 62% to 81% with analog proteins from apple (Mal d 3), apricot (Pru ar 3), plum (Pru d 3), cherry (Pru av 3), orange (Cit s 3), strawberry (Fra a 3), and grape (Vit v 1). Other LTPs with a structural homology with Pru p 3 are present in peanut (Ara h 9), wheat (Tri a 14), hazelnut (60% with Cor a 8), and walnut (66% with Jug r 3) [START_REF]Basic Local Alignment Search Tool[END_REF][START_REF] Matricardi | Plant non-specific lipid transfer proteins as food and pollen allergens[END_REF]. The risk of cross-reactivity most frequently involves the fruits of the Rosaceae family (apple, plum, apricot, cherry), but also nuts and peanuts. The clinical pattern ranges from local oropharyngeal symptoms up to anaphylaxis [START_REF] Matricardi | Plant non-specific lipid transfer proteins as food and pollen allergens[END_REF].

Co-factors are often involved (up to 40% of cases) in clinical expression: fasting, exercise, menstruation, and NSAID could determine the appearance of symptoms in patients sensitized to nsLTPs or influence symptom severity. According to Pascal et al., a cofactor is identified as precipitating anaphylaxis in 32.4% of nsLTPs allergic patients [START_REF] Pascal | Lipid transfer protein syndrome: Clinical pattern, cofactor effect and profile of molecular sensitization to plant-foods and pollens[END_REF].

Sensitization nsLTPs could also be involved in food-dependent exercise-induced anaphylaxis (FDEIA), provoked by the combination of food ingestion and physical exercise within 4 h of food ingestion and within one hour of the start of exercise [START_REF] Ukleja-Sokołowska | Food-dependent, exercise-induced anaphylaxis in a patient allergic to peach[END_REF].

In patients with peach-FDEIA, Pru p 3 is the most frequent sensitizer, followed by Pru p 7 [START_REF] Skypala | The diagnosis and management of allergic reactions in patients sensitized to non-specific lipid transfer proteins[END_REF][START_REF] Ukleja-Sokołowska | Food-dependent, exercise-induced anaphylaxis in a patient allergic to peach[END_REF][START_REF] Romano | Lipid transfer proteins: The most frequent sensitizer in Italian subjects with food-dependent exercise-induced anaphylaxis[END_REF].

Peamaclein Allergy

The peach allergen Pru p 7, also known as peamaclein, has recently been identified as a marker of peach allergy severity and as being responsible for peculiar clinical features, sometimes occurring in the presence of cofactors [START_REF] Klingebiel | Pru p 7 sensitization is a predominant cause of severe, cypress pollen-associated peach allergy[END_REF][START_REF] Sénéchal | A new allergen family involved in pollen food associated syndrome: Snakin/gibberellin regulated proteins[END_REF].

Peamaclein allergy is mostly observed in adolescents and adults. Pru p 7, similarly to Pru p 3, resists heat and digestion and it is suspected to cause a primary food allergy through the gastrointestinal tract route [START_REF] Inomata | Gibberellin-regulated protein in Japanese apricot is an allergen cross-reactive to Pru p 7[END_REF]. However, a recent study reported that sensitization to Pru p 7 develops in areas with high exposure to cypress pollen, due to the homology between Cypmaclein and Pru p 7, inducing a PFAS syndrome more severe than those previously described [START_REF] Klingebiel | Pru p 7 sensitization is a predominant cause of severe, cypress pollen-associated peach allergy[END_REF][START_REF] Sénéchal | A new allergen family involved in pollen food associated syndrome: Snakin/gibberellin regulated proteins[END_REF].

Moreover, Pru p 7 presents homology with Pru m 7 (Japanese apricot), Pun g 7 (pomegranate), Pru av 7 (cherry), and Cit s 7 (orange). In particular, Pru p 7 shows 100% sequence homology with Pru m 7, 97% with Pru av 7, 90% with Pun g 7, 87% with Cit s 7, 84% with black cottonwood GRP, 82% with potato GRP, and 81% with soybean GRP [START_REF] Inomata | Gibberellin-regulated protein allergy: Clinical features and cross reactivity[END_REF]. The clinical cross-reactivity between GRPs was reported among peach, Japanese apricot, orange, and pomegranate. In addition to these fruits, patients with GRP sensitization frequently experience allergic reactions against apple due to the presence of a GRP named applemeclein. It shares a 94% homology with Pru p 7 (peamaclein), Pru m 7 (Japanese apricot), and Pru av 7 (cherry) [START_REF] Tuppo | When the Frequencies of Sensitization and Elicitation of Allergic Reaction Do Not Correlate-The Case of Ap-ple Gibberellin-Regulated Protein Tested in an Italian Population[END_REF].

A recent multicenter study, including 316 subjects from France, reported that sensitization to Pru p 7 is common in peach-allergic subjects, with a prevalence of 62%, and it occurs often as monosensitization (54%). Furthermore, Pru p 7 sensitization and sIgE levels were higher in patients experiencing Grade 3 reactions, according to EAACI classification [START_REF] Klingebiel | Pru p 7 sensitization is a predominant cause of severe, cypress pollen-associated peach allergy[END_REF][START_REF] Muraro | The urgent need for a harmonized severity scoring system for acute allergic reactions[END_REF].

Swelling of the face, especially the eyelids, oropharyngeal tightness, and anaphylaxis featured with peamaclein allergy [START_REF] Inomata | Gibberellin-regulated protein in Japanese apricot is an allergen cross-reactive to Pru p 7[END_REF].

Inomata et al. observed, among peach-allergic patients sensitized to Pru p 7, that the most frequent symptoms were oropharyngeal (69.2%), followed by laryngeal tightness (46.2%), facial edema (46.2%), eyelid edema (46.2%), urticaria (38.5%), dyspnea (23.1%), nasal obstruction (23.1%), conjunctival injection (15.4%), lip edema (15.4%), loss of consciousness (15.4%), and hypotension (7.7%) [START_REF] Inomata | Eyelid edema as a predictive factor for sensitization to Pru p 7 in peach allergy[END_REF].

Diagnosis

As with any diagnostic workup for food allergy, screening allergen-sIgE testing without clinical necessity is discouraged [START_REF] Muraro | EAACI food allergy and anaphylaxis guidelines: Diagnosis and management of food allergy[END_REF][START_REF] Poncet | Update on pollen-food allergy syndrome[END_REF][START_REF] Burks | ICON: Food allergy[END_REF]. A detailed clinical history is therefore crucial for selecting the appropriate confirmatory tests. According to the ICON and EAACI guidelines for food allergies [START_REF] Muraro | EAACI food allergy and anaphylaxis guidelines: Diagnosis and management of food allergy[END_REF], the diagnosis of peach allergy lies on the combination of a convincing clinical history of immediate reaction to peach and positive IgE sensitization testing assessed through SPT to peach (in the form of either extract, molecular components, or fresh peach), and/or IgE sensitization to peach (either extracts or molecular components). Where the diagnosis is unclear, an oral food challenge (OFC) is required as the gold standard test to provide a definitive diagnosis and to prevent patients from unneeded and potentially harmful elimination diets. However, OFC is logistically demanding, and anaphylactic reactions may occur. Reliable prognostic markers or algorithms integrating different clinical and biological parameters for predicting the severity of allergic reactions during OFC are under investigation.

Clinical History

A convincing clinical history is usually defined as one or more immediate reaction(s) within two hours of peach ingestion, inhalation, or direct contact, presenting as acute urticaria or angioedema, contact urticaria, laryngeal swelling, immediate vomiting, rhinitis, cough, wheezing, bronchospasm, hypotension or loss of consciousness, oral allergy syndrome (i.e., itching and tingling of the lips, oral mucosa and/or tongue), or food-dependent exercise-induced anaphylaxis [START_REF] Muraro | EAACI food allergy and anaphylaxis guidelines: Diagnosis and management of food allergy[END_REF][START_REF] Werfel | Position paper of the EAACI: Food allergy due to immunological cross-reactions with common inhalant allergens[END_REF][START_REF] Burks | ICON: Food allergy[END_REF]. The severity of reactions is useful for suspecting specific patterns of sensitization and proper management. Peamaclein (Pru p 7) frequently elicits anaphylaxis [START_REF] Inomata | Identification of peamaclein as a marker allergen related to systemic reactions in peach allergy[END_REF] and, similarly to allergy to other gibberellins, often includes peculiar clinical symptoms, such as facial swelling and laryngeal tightness, which can be predictive factors for gibberellin allergies [START_REF] Inomata | Gibberellin-regulated protein in Japanese apricot is an allergen cross-reactive to Pru p 7[END_REF]. Because of their labile chemical structure, profilins (Pru p 4 in peach) and PR-10 (Pru p 1 in peach) are usually responsible for mild symptoms [START_REF] Matricardi | EAACI Molecular Allergology User's Guide[END_REF]. Cofactors should be always investigated (e.g., asthma exacerbations, infections, exercise, alcohol, tiredness, use of NSAIDs, and menstruation), since they usually play a crucial role in eliciting reactions in patients allergic to nsLTP (Pru p 3 in peach) and, less frequently, in patients allergic to gibberellins (more evident for Pru m 7 (apricot) and Cit s 7 (orange) less for Pru p 7 (peach)). In t patients who have peach-FDEIA, nsLTPs are the most frequent sensitizers, followed by peamaclein [START_REF] Skypala | The diagnosis and management of allergic reactions in patients sensitized to non-specific lipid transfer proteins[END_REF].

Therefore, the clinical history should include the following: possible causative food(s) (peach and other fruits/vegetables), the time of onset, the extent and reproducibility of symptoms, the identification of allergic symptoms with plants and plants food(s), the quantity of ingested food, details of the food preparation (e.g., raw vs. cooked, peeled vs. unpeeled), and the relevance of cofactors.

IgE Sensitization

The use of peach-specific IgE determination combined with clinical history and peach SPT may reduce the need for OFC [START_REF] Muraro | Precision medicine in allergic disease-food allergy, drug allergy, and anaphylaxis-PRACTALL document of the European Academy of Allergy and Clinical Immunology and the American Academy of Allergy, Asthma and Immunology[END_REF]. Component-resolved diagnosis (CRD), which uses single allergenic components for the assessment of epitope-sIgE, can provide critical information for predicting individualized sensitization patterns and the risk of severe allergic reactions [START_REF] Matricardi | EAACI Molecular Allergology User's Guide[END_REF]. Only molecular diagnostics makes it possible to identify and differentiate sensitization to LTP or peamaclein. Peach LTP extracts for SPT are contaminated with peamaclein Pru p 7, because LTP Pru p 3 and peamaclein Pru p 7 have similar molecular weights.

The use of commercial peach extracts for SPT is useful in clinical practice. However, clinicians should consider that peach extract for SPT most likely lacks labile peach allergens (i.e., Pru p 1, and Pru p 4), because these are usually lost during production procedures. By contrast, stable allergens, such as Pru p 3 and Pru p 7, are usually retained in commercial peach extracts [START_REF] Tuppo | Peamaclein-A new peach allergenic protein: Similarities, differences and misleading features compared to Pru p 3[END_REF]. Consequently, SPT with current extracts may furnish a prompt, firstlevel, component-resolved diagnosis at the bedside [START_REF] Asero | Evaluation of two commercial peach extracts for skin prick testing in the diagnosis of hypersensitivity to lipid transfer protein. A multicenter study[END_REF][START_REF] Somoza | Skin Testing With Peach Peel Extract Versus Serum IgE to Pru p 3 as a Stronger Predictor of Peach-Induced Anaphylaxis[END_REF].

The use of serum-sIgE against molecular components provides useful support to the diagnosis and may help with risk stratification, assessment, and management. Pru p 7 is a small protein that is upregulated upon biotic stress. It represents a major allergen associated with severe clinical symptoms and strong cypress pollen sensitization [START_REF] Klingebiel | Pru p 7 sensitization is a predominant cause of severe, cypress pollen-associated peach allergy[END_REF]. A study conducted in the southern part of France evaluated 316 patients with suspected peach allergy. According to the ICON and EAACI guidelines for food allergies, peach allergy was diagnosed in 198 subjects. Sensitization to Pru p 7 was present in 171 (54%) of all the subjects in the study and 123 of 198 (62%) were diagnosed as peach-allergic, more than half of whom were sensitized to no other peach allergen. The frequency and magnitude of Pru p 7 sensitization were associated with the presence of a peach allergy, the clinical severity of peach-induced allergic reactions, and the level of cypress pollen exposure. Cypress pollen extract completely outcompeted IgE binding to Pru p 7 [START_REF] Allergy | f95 Peach[END_REF].

Oral Food Challenge

If the diagnosis of peach allergy is in doubt, OFC is required as it represents the gold standard for the diagnosis of any food allergy. Some OFC protocols are intended to test peach peel and pulp separately [START_REF] Klingebiel | Pru p 7 sensitization is a predominant cause of severe, cypress pollen-associated peach allergy[END_REF], others to test them both, and some to assess exercise-induced anaphylaxis [START_REF] Kennard | A Multicenter Evaluation of Diagnosis and Management of Omega-5 Gliadin Allergy (Also Known as Wheat-Dependent Exercise-Induced Anaphylaxis) in 132 Adults[END_REF]. Furthermore, clinicians may consider allergy testing and, ultimately, OFC to plant foods containing nsLTPs or GRP with known potential crossreactivity with peach if oral tolerance to these foods is in doubt, and according to the patient's preference (Figure 1). reactivity with peach if oral tolerance to these foods is in doubt, and according to the patient's preference (Figure 1).

Prevention and Management

Primary and Secondary Prevention

To date, no study has shown a possible effective strategy for the primary prevention of peach allergy. Neither polyunsaturated fatty acid supplementation during pregnancy nor the use of probiotics and fish oil supplementation in infancy were effective at preventing the appearance of food allergies [START_REF] O'keefe | Diagnosis and management of food allergies: New and emerging options: A systematic review[END_REF]. The early introduction of food during diversification could be a possible primary prevention strategy [START_REF] Caimmi | Food allergy in primary care[END_REF]. Even though current data show moderate evidence that the early introduction of peanut and egg reduces the risk of food allergy, there is no sufficient scientific information on other major food allergens [START_REF] Fleischer | A consensus approach to the primary prevention of food allergy through nutrition: Guidance from the American Academy of Allergy, Asthma, and Immunology; American College of Allergy, Asthma, and Immunology; and the Canadian Society for Allergy and Clinical Immunology[END_REF].

In peach-allergic patients, as with other food allergies, prescribing preventive antihistamines was not shown to be effective at preventing possible allergic reactions; furthermore, this strategy may delay the timely and appropriate use of adrenaline to treat anaphylaxis [START_REF] Muraro | EAACI food allergy and anaphylaxis guidelines: Diagnosis and management of food allergy[END_REF]. The use of mast-cell stabilizers to prevent allergic reactions showed different clinical results, making it not advisable, so far, as a prophylactic strategy for food allergies in general and, therefore, for peach allergy as well [START_REF] Muraro | EAACI food allergy and anaphylaxis guidelines: Diagnosis and management of food allergy[END_REF]. The use of monoclonal antibodies, such as omalizumab and dupilumab, has been suggested instead in the treatment of food allergies, mostly as adjuvant therapy for immunotherapy rather than as a possible preventive strategy against the development of clinical symptoms in allergic patients [START_REF] Fiocchi | The use of biologics in food allergy[END_REF].

Prevention and Management

Primary and Secondary Prevention

To date, no study has shown a possible effective strategy for the primary prevention of peach allergy. Neither polyunsaturated fatty acid supplementation during pregnancy nor the use of probiotics and fish oil supplementation in infancy were effective at preventing the appearance of food allergies [START_REF] O'keefe | Diagnosis and management of food allergies: New and emerging options: A systematic review[END_REF]. The early introduction of food during diversification could be a possible primary prevention strategy [START_REF] Caimmi | Food allergy in primary care[END_REF]. Even though current data show moderate evidence that the early introduction of peanut and egg reduces the risk of food allergy, there is no sufficient scientific information on other major food allergens [START_REF] Fleischer | A consensus approach to the primary prevention of food allergy through nutrition: Guidance from the American Academy of Allergy, Asthma, and Immunology; American College of Allergy, Asthma, and Immunology; and the Canadian Society for Allergy and Clinical Immunology[END_REF].

In peach-allergic patients, as with other food allergies, prescribing preventive antihistamines was not shown to be effective at preventing possible allergic reactions; furthermore, this strategy may delay the timely and appropriate use of adrenaline to treat anaphylaxis [START_REF] Muraro | EAACI food allergy and anaphylaxis guidelines: Diagnosis and management of food allergy[END_REF]. The use of mast-cell stabilizers to prevent allergic reactions showed different clinical results, making it not advisable, so far, as a prophylactic strategy for food allergies in general and, therefore, for peach allergy as well [START_REF] Muraro | EAACI food allergy and anaphylaxis guidelines: Diagnosis and management of food allergy[END_REF]. The use of monoclonal antibodies, such as omalizumab and dupilumab, has been suggested instead in the treatment of food allergies, mostly as adjuvant therapy for immunotherapy rather than as a possible preventive strategy against the development of clinical symptoms in allergic patients [START_REF] Fiocchi | The use of biologics in food allergy[END_REF].

Management of Peach Allergy

Once the diagnosis of peach allergy is made, peach should be eliminated from the patient's diet. Foods possibly cross-reacting with peach allergens should also be investigated by, firstly, assessing whether the patient is exposed to these foods without presenting symptoms and, if this is found not to be the case, by performing skin tests and/or specific IgE dosing. This strategy should mainly be considered for food cross-reacting via Pru p 3 (LTP) or Pru p 7 (peamaclein), given the higher risk of severe reaction associated with sensitization to these allergens.

Management strategies should include both the management of acute accidental reactions and long-term avoidance strategies.

A written emergency action plan for acute reactions should always be provided to all patients with peach allergy. In addition, two adrenaline auto-injectors (AAI) should be prescribed to patients with a history of anaphylaxis to peach.

In order to properly avoid peach, patients should also be educated on how to recognize the presence of peach in commercial products (such as fruit juices). Unfortunately, current labeling practices and legislation do not include the obligation to include the presence of this food, nor to highlight it on the label [START_REF]EU Law on Food Information to Consumers[END_REF], which could result in the threat of accidental exposure. Other important aspects of educational programs for peach allergy include understanding and recognizing the early signs/symptoms of a possible allergic reaction, avoiding possible triggers or cofactors able to elicit the allergic reaction (e.g., asthma exacerbations, infections, exercise, alcohol, tiredness, use of NSAIDs, and menstruation), and knowing when and how to administer proper treatment, especially if an adrenaline auto-injector has been prescribed [START_REF] Biagioni | Allergy to Gibberellin-Regulated Proteins (Peamaclein) in Children[END_REF][START_REF] Muraro | EAACI food allergy and anaphylaxis guidelines: Diagnosis and management of food allergy[END_REF][START_REF] Caimmi | Food allergy in primary care[END_REF].

Allergen Immunotherapy

Immunotherapy is considered an attractive option to treat food allergies and aims at inducing immunological tolerance (the possibility of safe consumption, regardless of regular exposure) of foods.

In terms of oral immunotherapy (OIT), increasing amounts of food are administered to patients with a proven allergy in order to induce desensitization and, possibly, tolerance. In a paper by Patriarca et al. [START_REF] Patriarca | Oral desensitizing treatment in food allergy: Clinical and immunological results[END_REF], one adult patient underwent OIT with peach and was successfully treated over a 3-month period. Nevertheless, the authors did not provide more specific details on this patient. A more recent study proposed a protocol using peach juice in 24 peach-allergic patients; the protocol followed a sublingual immunotherapy (SLIT) strategy [START_REF] Navarro | Oral immunotherapy with peach juice in patients allergic to LTPs[END_REF]. At the end of the study, the authors were able to administer 200 mL of peach juice to 70.8% of their patients, without reporting severe adverse reactions during the challenge [START_REF] Navarro | Oral immunotherapy with peach juice in patients allergic to LTPs[END_REF]. In any case, peach, as a wholly allergenic source, has not been an allergen on which researchers have focused their attention, as has been the case with OIT. Other SLIT protocols using specific peach proteins have been proposed, such as Pru p 3, on which several studies have been conducted. In the first published study on this topic, after 6 months of SLIT with peach extract quantified in mass units for Pru p 3, 33 patients showed an increase of 3-to-9 fold in their eliciting dose, with a significant difference when compared with the placebo group; moreover, no serious adverse events were reported, and the patients mainly experienced local reactions [START_REF] Fernández-Rivas | Randomized double-blind, placebo-controlled trial of sublingual immunotherapy with a Pru p 3 quantified peach extract[END_REF]. A more recent paper confirmed these results on 15 patients, even with an ultra-rush protocol [START_REF] Moura | Pru p 3 sublingual immunotherapy ultra-rush protocol is safe and clinically effective[END_REF]. Furthermore, Beitia et al. showed the effectiveness of Pru p 3 SLIT in a real-life study, including 29 patients, showing that, one year after starting SLIT, 73% had a negative challenge to peach, and, after 2 years, 95% of them did not react to the fruit [START_REF] Beitia | Pru p 3 sublingual immunotherapy in patients with Lipid Tranfer Protein Syndrome: Is it worth?[END_REF]. In this study, the possibility of using Pru p 3 SLIT to treat patients suffering from LTP syndrome was confirmed, as also shown in other papers, with a positive impact on patients' quality of life as well [START_REF] González Pérez | Pru p 3 oral immunotherapy efficacy, induced immunological changes and quality of life improvement in patients with LTP syndrome[END_REF][START_REF] García-Gutiérrez | Treatment with lipid transfer protein sublingual immunotherapy: Slowing down new sensitizations[END_REF]. Indeed, in the paper by González-Pérez et al., the authors showed that, in 18 adult patients treated for 3 consecutive years with Pru p 3 SLIT, the results on the Food Allergy Quality of Life Questionnaire-Adult Form (FAQLQ-AF) significantly decreased, showing a favorable impact on the patients' quality of life [START_REF] González Pérez | Pru p 3 oral immunotherapy efficacy, induced immunological changes and quality of life improvement in patients with LTP syndrome[END_REF].

Finally, for patients suffering from PFAS, some authors focused on the possibility of treatment with subcutaneous immunotherapy (SCIT), using birch pollen extract. Nevertheless, researchers showed controversial results on this specific issue [START_REF] Caimmi | Guidelines for the prescription of allergen immunotherapy and patient's follow-up-Clinical questions and revision of the literature[END_REF][START_REF] Hamada | Evaluation of subcutaneous immunotherapy with birch pollen extract for pollen-food allergy syndrome[END_REF][START_REF] Van Der Valk | The Effect of Birch Pollen Immunotherapy on Apple and rMal d 1 Challenges in Adults with Apple Allergy[END_REF], and no study was specifically conducted on peach-allergic patients.

In general, even though peach OIT is possibly administered in research and specialized settings, there are currently insufficient data to be able to recommend this approach to treating patients in clinical practice [START_REF] Pajno | EAACI Guidelines on allergen immunotherapy: IgE-mediated food allergy[END_REF].

Conclusions

Peach allergy may manifest with different clinical symptoms of ranging severity. Based on patients' sensitization profiles, clinicians may be able to highlight which patients are more at risk of developing a severe allergic reaction. Unfortunately, in clinical practice, clinicians are only able to dose specific serum IgE for the whole peach source, and for Pru p 1 (PR-10), Pru p 3 (LTP), Pru p 4 (profiline), and Pru p 7 (peamaclein). Patients monosensitized to Pru p 9 are known to be at risk of respiratory symptoms, while patients allergic to Pru p 3 and/or Pru p 7 are at risk of experiencing severe allergic reactions. To properly diagnose a peach allergy, therefore, besides presenting a compatible clinical history related to the fruit, patients need to have positive SPT and/or sIgE to available allergens. In cases in which diagnosis cannot be reached by combining these tests, an OFC may be performed, as this procedure is still considered the gold diagnostic standard. Once the diagnosis is made, patients and caregivers should receive proper education on peach avoidance strategies, an emergency action plan for accidental acute reactions and AAIs in case of history of anaphylaxis to peach. OIT is a promising treatment for patients with food allergies who are at high risk of a life-threatening reaction or severe impairment of quality of life. However, currently, peach immunotherapy is not advised in clinical practice.

Figure 1 .

 1 Figure 1. Diagnostic algorithm for peach allergy. Adapted from [40].

Figure 1 .

 1 Figure 1. Diagnostic algorithm for peach allergy. Adapted from [40].

Acknowledgments:

The authors want to thank the Italian Society of Pediatric Allergy and Immunology (Societa' italiana di allergologia e Immunologia pediatrica, SIAIP, Via Libero Temolo 4, 20126 Milano) for its support in relation to this work.

Data Availability Statement: Not applicable.

Funding:

The publication fee was financed by the Italian Society of Pediatric Allergy and Immunology (Societa' italiana di allergologia e Immunologia pediatrica, SIAIP, Via Libero Temolo 4, 20126 Milano). However, no significant funding source could have influenced the outcomes of this work.

Institutional Review Board Statement: Not applicable.

Informed Consent Statement: Not applicable.

Conflicts of Interest:

The authors declare no conflict of interest.