Noelia M Sanchez-Ballester
email: noelia.sanchez-ballester@umontpellier.fr

Flavien Sciortino
email: flavien.sciortino@unibas.ch

Sajjad Husain Mir
email: sajjad.mir@tcd.ie

Gaulthier Rydzek
email: gaulthier.rydzek@umontpellier.fr

Weak Polyelectrolytes as Nanoarchitectonic Design Tools for Functional Materials: A Review of Recent Achievements

Keywords: polymer materials, polyelectrolyte films, electrodeposition, block copolymer, self-assembly, LbL, nanostructured materials, pharmaceutical vectors

The ionization degree, charge density, and conformation of weak polyelectrolytes can be adjusted through adjusting the pH and ionic strength stimuli. Such polymers thus offer a range of reversible interactions, including electrostatic complexation, H-bonding, and hydrophobic interactions, which position weak polyelectrolytes as key nano-units for the design of dynamic systems with precise structures, compositions, and responses to stimuli. The purpose of this review article is to discuss recent examples of nanoarchitectonic systems and applications that use weak polyelectrolytes as smart components. Surface platforms (electrodeposited films, brushes), multilayers (coatings and capsules), processed polyelectrolyte complexes (gels and membranes), and pharmaceutical vectors from both synthetic or natural-type weak polyelectrolytes are discussed. Finally, the increasing significance of block copolymers with weak polyion blocks is discussed with respect to the design of nanovectors by micellization and film/membrane nanopatterning via phase separation.

Context

Functional materials with precisely controlled compositions and (nano)structures and predictable responses to stimuli are prime candidates to address a number of challenges in fields including biomaterials, vectorization, and energy storage and conversion [START_REF] Ariga | Progress in Molecular Nanoarchitectonics and Materials Nanoarchitectonics[END_REF]. The development of such "smart" materials relies largely on designing functional systems through the assembly of well-controlled nanoscale units. In this context, the paradigm of "nanoarchitectonics" was proposed by Aono in the early 2000s [START_REF] Aono | Nanoarchitectonics: Pioneering a New Paradigm for Nanotechnology in Materials Development[END_REF] and subsequently developed by Ariga to enable materials that benefit from (i) the emergence of system-scale functions from the collective integration of nano-units (nanosystem functionality), (ii) robust function despite nano-unit/assembly defects (unreliability-tolerant reliability), and (iii) the emergence of unexpected new functions from large combinatorial systems (quantity changes quality). Thus, implementing this concept by combining tools from research fields including nanotechnology, (bio)material science, and organic and polymer chemistry has ensured that considerable advances have been made in the elaboration of dynamic materials and systems relying on reversible non-covalent interactions [START_REF] Ariga | Nanoarchitectonics Revolution and Evolution: From Small Science to Big Technology[END_REF]. Typically, the reversible nature of interactions with and within functional nano-units provides materials with self-assembly synthesis routes, structural control, encapsulation abilities, and responses to stimuli [START_REF] Ariga | Intelligent Nanoarchitectonics for Self-Assembling Systems[END_REF]. In this context, polyelectrolytes occupy a central position in many nanoarchitectured systems owing to their multipotent properties, enabling them to act as reticulation/bridging agents, stabilizers, structure directors, and matrix components in functional coatings, gels, and colloidal systems [START_REF] Ariga | Intelligent Nanoarchitectonics for Self-Assembling Systems[END_REF][START_REF] Vranckx | Layer-by-Layer Nanoarchitectonics Using Protein-Polyelectrolyte Complexes toward a Generalizable Tool for Protein Surface Immobilization[END_REF].

Polyelectrolytes whose charge does not change over the entire pH range [START_REF] Ariga | Progress in Molecular Nanoarchitectonics and Materials Nanoarchitectonics[END_REF][START_REF] Aono | Nanoarchitectonics: Pioneering a New Paradigm for Nanotechnology in Materials Development[END_REF][START_REF] Ariga | Nanoarchitectonics Revolution and Evolution: From Small Science to Big Technology[END_REF][START_REF] Ariga | Intelligent Nanoarchitectonics for Self-Assembling Systems[END_REF][START_REF] Vranckx | Layer-by-Layer Nanoarchitectonics Using Protein-Polyelectrolyte Complexes toward a Generalizable Tool for Protein Surface Immobilization[END_REF][START_REF] Rathee | Explicit Ion Effects on the Charge and Conformation of Weak Polyelectrolytes[END_REF][START_REF] Yu | Multivalent Counterions Diminish the Lubricity of Polyelectrolyte Brushes[END_REF][START_REF] Sciortino | Saloplastics as Multiresponsive Ion Exchange Reservoirs and Catalyst Supports[END_REF][START_REF] Neitzel | Expanding the Structural Diversity of Polyelectrolyte Complexes and Polyzwitterions[END_REF][START_REF] Wang | Charge on a Weak Polyelectrolyte[END_REF][START_REF] Yang | Precision Doping of Polyelectrolyte Complexes: Insight on the Role of Ions[END_REF][START_REF] Digby | Salt Resistance as a Measure of the Strength of Polyelectrolyte Complexation[END_REF][START_REF] Durmaz | Weak Polyanion and Strong Polycation Complex Based Membranes: Linking Aqueous Phase Separation to Traditional Membrane Fabrication[END_REF][START_REF] Porcel | Compact Polyelectrolyte Complexes: "Saloplastic" Candidates for Biomaterials[END_REF] are considered "strong", while the density of charge on "weak" polyelectrolytes depends on the pH. In such a case, weak polyanions (resp. polycations) bear weak acid (resp. basic) moieties and their pKa value corresponds to the pH at which half of their repeating monomer units are charged. Consequently, the ionization degree of weak polyelectrolytes is intrinsically pH-responsive, with consequences for their conformation (such as extended chains being favored at high ionization degrees), H-bonding (being favored at low ionization degrees), and electrostatic complexation capabilities with other charged species (Scheme 1). In addition, the apparent charge density of weak polyelectrolytes can be modulated through ion screening by changing the concentration of monovalent salts in solutions. This phenomenon, which represents a second type of response to stimuli, disfavors the electrostatic complexation of polyelectrolytes at high ionic strengths and changes their conformation, with swollen loops being favored at high ionic strengths [START_REF] Rathee | Explicit Ion Effects on the Charge and Conformation of Weak Polyelectrolytes[END_REF]. Finally, when the valency of counter-ions is changed from monovalent to multivalent, intramolecular electrostatic crosslinking leads to further conformational transitions (Scheme 1) [START_REF] Yu | Multivalent Counterions Diminish the Lubricity of Polyelectrolyte Brushes[END_REF][START_REF] Sciortino | Saloplastics as Multiresponsive Ion Exchange Reservoirs and Catalyst Supports[END_REF]. It is thus not surprising that a wide range of synthetic and natural weak polyelectrolytes have been developed (Table 1), while their properties as reversible polyacids/bases [START_REF] Neitzel | Expanding the Structural Diversity of Polyelectrolyte Complexes and Polyzwitterions[END_REF][START_REF] Wang | Charge on a Weak Polyelectrolyte[END_REF], complexing agents [START_REF] Yang | Precision Doping of Polyelectrolyte Complexes: Insight on the Role of Ions[END_REF][START_REF] Digby | Salt Resistance as a Measure of the Strength of Polyelectrolyte Complexation[END_REF], and self-assembling units in functional systems [START_REF] Durmaz | Weak Polyanion and Strong Polycation Complex Based Membranes: Linking Aqueous Phase Separation to Traditional Membrane Fabrication[END_REF][START_REF] Porcel | Compact Polyelectrolyte Complexes: "Saloplastic" Candidates for Biomaterials[END_REF][START_REF] Geoghegan | Weak Polyelectrolyte Brushes[END_REF][START_REF] Decher | Fuzzy Nanoassemblies: Toward Layered Polymeric Multicomposites[END_REF] have been very actively investigated. It is worth noting that the apparent pKa of weak polyelectrolytes, measured by pH titration, conductimetry, and infrared spectroscopy, varies notably depending on experimental parameters such as their molecular weight, conformation, confinement, and complexation [START_REF] Dolce | Ionization of Short Weak Polyelectrolytes: When Size Matters[END_REF][START_REF] Landsgesell | Simulation of Weak Polyelectrolytes: A Comparison between the Constant PH and the Reaction Ensemble Method[END_REF][START_REF] Carnal | Chain Stiffness, Salt Valency, and Concentration Influences on Titration Curves of Polyelectrolytes: Monte Carlo Simulations[END_REF][START_REF] Brilmayer | Influence of Nanoconfinement on the PKa of Polyelectrolyte Functionalized Silica Mesopores[END_REF].

Molecules 2022, 27, x FOR PEER REVIEW 2 of 32 position in many nanoarchitectured systems owing to their multipotent properties, enabling them to act as reticulation/bridging agents, stabilizers, structure directors, and matrix components in functional coatings, gels, and colloidal systems [START_REF] Ariga | Intelligent Nanoarchitectonics for Self-Assembling Systems[END_REF][START_REF] Vranckx | Layer-by-Layer Nanoarchitectonics Using Protein-Polyelectrolyte Complexes toward a Generalizable Tool for Protein Surface Immobilization[END_REF].

Polyelectrolytes whose charge does not change over the entire pH range [START_REF] Ariga | Progress in Molecular Nanoarchitectonics and Materials Nanoarchitectonics[END_REF][START_REF] Aono | Nanoarchitectonics: Pioneering a New Paradigm for Nanotechnology in Materials Development[END_REF][START_REF] Ariga | Nanoarchitectonics Revolution and Evolution: From Small Science to Big Technology[END_REF][START_REF] Ariga | Intelligent Nanoarchitectonics for Self-Assembling Systems[END_REF][START_REF] Vranckx | Layer-by-Layer Nanoarchitectonics Using Protein-Polyelectrolyte Complexes toward a Generalizable Tool for Protein Surface Immobilization[END_REF][START_REF] Rathee | Explicit Ion Effects on the Charge and Conformation of Weak Polyelectrolytes[END_REF][START_REF] Yu | Multivalent Counterions Diminish the Lubricity of Polyelectrolyte Brushes[END_REF][START_REF] Sciortino | Saloplastics as Multiresponsive Ion Exchange Reservoirs and Catalyst Supports[END_REF][START_REF] Neitzel | Expanding the Structural Diversity of Polyelectrolyte Complexes and Polyzwitterions[END_REF][START_REF] Wang | Charge on a Weak Polyelectrolyte[END_REF][START_REF] Yang | Precision Doping of Polyelectrolyte Complexes: Insight on the Role of Ions[END_REF][START_REF] Digby | Salt Resistance as a Measure of the Strength of Polyelectrolyte Complexation[END_REF][START_REF] Durmaz | Weak Polyanion and Strong Polycation Complex Based Membranes: Linking Aqueous Phase Separation to Traditional Membrane Fabrication[END_REF][START_REF] Porcel | Compact Polyelectrolyte Complexes: "Saloplastic" Candidates for Biomaterials[END_REF] are considered "strong", while the density of charge on "weak" polyelectrolytes depends on the pH. In such a case, weak polyanions (resp. polycations) bear weak acid (resp. basic) moieties and their pKa value corresponds to the pH at which half of their repeating monomer units are charged. Consequently, the ionization degree of weak polyelectrolytes is intrinsically pH-responsive, with consequences for their conformation (such as extended chains being favored at high ionization degrees), H-bonding (being favored at low ionization degrees), and electrostatic complexation capabilities with other charged species (Scheme 1). In addition, the apparent charge density of weak polyelectrolytes can be modulated through ion screening by changing the concentration of monovalent salts in solutions. This phenomenon, which represents a second type of response to stimuli, disfavors the electrostatic complexation of polyelectrolytes at high ionic strengths and changes their conformation, with swollen loops being favored at high ionic strengths [START_REF] Rathee | Explicit Ion Effects on the Charge and Conformation of Weak Polyelectrolytes[END_REF]. Finally, when the valency of counter-ions is changed from monovalent to multivalent, intramolecular electrostatic crosslinking leads to further conformational transitions (Scheme 1) [START_REF] Yu | Multivalent Counterions Diminish the Lubricity of Polyelectrolyte Brushes[END_REF][START_REF] Sciortino | Saloplastics as Multiresponsive Ion Exchange Reservoirs and Catalyst Supports[END_REF]. It is thus not surprising that a wide range of synthetic and natural weak polyelectrolytes have been developed (Table 1), while their properties as reversible polyacids/bases [START_REF] Neitzel | Expanding the Structural Diversity of Polyelectrolyte Complexes and Polyzwitterions[END_REF][START_REF] Wang | Charge on a Weak Polyelectrolyte[END_REF], complexing agents [START_REF] Yang | Precision Doping of Polyelectrolyte Complexes: Insight on the Role of Ions[END_REF][START_REF] Digby | Salt Resistance as a Measure of the Strength of Polyelectrolyte Complexation[END_REF], and self-assembling units in functional systems [START_REF] Durmaz | Weak Polyanion and Strong Polycation Complex Based Membranes: Linking Aqueous Phase Separation to Traditional Membrane Fabrication[END_REF][START_REF] Porcel | Compact Polyelectrolyte Complexes: "Saloplastic" Candidates for Biomaterials[END_REF][START_REF] Geoghegan | Weak Polyelectrolyte Brushes[END_REF][START_REF] Decher | Fuzzy Nanoassemblies: Toward Layered Polymeric Multicomposites[END_REF] have been very actively investigated. It is worth noting that the apparent pKa of weak polyelectrolytes, measured by pH titration, conductimetry, and infrared spectroscopy, varies notably depending on experimental parameters such as their molecular weight, conformation, confinement, and complexation [START_REF] Dolce | Ionization of Short Weak Polyelectrolytes: When Size Matters[END_REF][START_REF] Landsgesell | Simulation of Weak Polyelectrolytes: A Comparison between the Constant PH and the Reaction Ensemble Method[END_REF][START_REF] Carnal | Chain Stiffness, Salt Valency, and Concentration Influences on Titration Curves of Polyelectrolytes: Monte Carlo Simulations[END_REF][START_REF] Brilmayer | Influence of Nanoconfinement on the PKa of Polyelectrolyte Functionalized Silica Mesopores[END_REF].

Scheme 1. Responses of weak polyelectrolytes to stimuli. Schematic depiction of the molecular and conformational responses of weak polyelectrolytes bearing either weak acid (AH) or basic (B) moieties with respect to pH and salt stimuli. Table 1. Most common weak polyelectrolytes and their pKa/pKaH. * When available, the apparent pKa/pKaH of the polyelectrolyte is mentioned in parentheses. Note that these values may vary with the polyelectrolyte's molecular weight and environment [START_REF] Dolce | Ionization of Short Weak Polyelectrolytes: When Size Matters[END_REF]. Table 1. Most common weak polyelectrolytes and their pKa/pKaH. * When available, the apparent pKa/pKaH of the polyelectrolyte is mentioned in parentheses. Note that these values may vary with the polyelectrolyte's molecular weight and environment [START_REF] Dolce | Ionization of Short Weak Polyelectrolytes: When Size Matters[END_REF]. 4.5-6.6 up to 6.8 [21,22] [21] Natural polyanions

Type

Type

Poly(glutamic acid) (PGA) Hyaluronic acid (HA) Alginic acid (ALG)

2.1 ≈3.0 3.5-4.6 6.1 [21] [23] [START_REF] López-García | New Polymeric/Inorganic Hybrid Sorbents Based on Red Mud and Nanosized Magnetite for Large Scale Applications in As(V) Removal[END_REF] Synthetic polycations Poly(allylamine hydrochloride) (PAH) Poly(aniline) (PANI) Poly(ethyleneimine) (PEI) Poly(2-vinylpyridine) (P2VP) Poly (2-(dimethylamino)ethyl methacrylate) (PDMAEMA) Poly(L-lysine) (PLL) 9.7 4.6 8.0 5-5. [START_REF] Rathee | Explicit Ion Effects on the Charge and Conformation of Weak Polyelectrolytes[END_REF] 8.4 10.5

8.6 5.5 8.2-9.9 3.5-4.5 ≈7.5 10-10.5 [25] [26] [27] [28,29] [20,30] [31,32] Natural polycations Chitosan (CHI) 7.5 ~6.5 [START_REF] Mohammed | An Overview of Chitosan Nanoparticles and Its Application in Non-Parenteral Drug Delivery[END_REF] Based on these backgrounds, weak polyelectrolytes offer a range of non-covalent interactions, including electrostatic, H-bonding, and hydrophobic interactions. On the one hand, these properties can not only ensure the cohesion of polymeric matrices, films, and colloids but also direct their structuration at the nano and micron levels (Figure 1). On the other hand, they offer routes to tailor the functions-including sorption, swelling, and mechanical responses-of systems whose cohesion relies on covalent bonds. The objective of this review article is to present recent examples of stimuli-responsive systems with triggerable interactions at the molecular and supramolecular levels by modulating the charge density on weak polyelectrolytes through salt and pH stimuli. The examples described cannot cover all aspects but rather focus on the most recent developments, generally reported less than 5 years ago. Accordingly, this review is organized into four sections, each dealing with a different nature of weak polyelectrolyte systems: -

In the initial part, organic and hybrid thin films obtained from weak polyelectrolyte brushes and electrodeposited coatings are described, including those based on natural polyelectrolytes (e.g., chitosan, alginate, hyaluronic acid). The cohesion of such systems relies on covalent, hydrophobic and H-bonding interactions, most often leading to coatings containing one weak polyelectrolyte at a time. Basic synthesis approaches for these films are discussed as well as their applications, with an emphasis on their response to post-assembly pH changes in terms of swelling, adhesion, and cargo encapsulation and release. -

The next two sections describe systems based on polyelectrolyte complexes, including those formed from the electrostatic complexation of weak polyanions and polycations [START_REF] Fu | Driving Forces for Oppositely Charged Polyion Association in Aqueous Solutions: Enthalpic, Entropic, but Not Electrostatic[END_REF]. Their assembly in aqueous media, without aggressive chemicals, allows for the use of more environmentally friendly routes to obtain surface coatings (films and layer-by-layer capsules), vectors, and functional gels. The resulting systems usually include several polyelectrolytes at the same time, and their cohesion is based on reversible interactions. The fundamentals of these films, gels, and colloids are discussed with respect to their response to pH and salt stimuli both during and after assembly. Emphasis is placed on recently proposed processing strategies to transform electrostatic complexes into gels and membranes. The applications of these systems are reviewed with a focus on nanovectors, and a subsection is devoted to systems that have been identified as relevant to pharmaceutical needs. -

The final part addresses the growing significance of block copolymers (BCP) containing weak polyelectrolyte blocks for nanostructuring surfaces, colloids, and membranes. Accordingly, their directed self-assembly into microphase-separated films and their micellization behavior are discussed as a function of complexation, pH, and salt stimuli. Emerging applications, including sensors, nanolithography, and vectorization, are discussed.

Weak Polyelectrolytes Layers for Stimuli-Responsive Surfaces

Coatings based on weak polyelectrolytes provide researchers with a convenient way to tune the properties of surfaces, ultimately adjusting their interactions through their environment and enabling the design of stimuli-responsive systems. This section aims to review brushes and electrodeposited coatings, most often composed of only one weak polyelectrolyte, as well as their applications with respect to their response to pH changes

Weak Polyelectrolytes Layers for Stimuli-Responsive Surfaces

Coatings based on weak polyelectrolytes provide researchers with a convenient way to tune the properties of surfaces, ultimately adjusting their interactions through their environment and enabling the design of stimuli-responsive systems. This section aims to review brushes and electrodeposited coatings, most often composed of only one weak polyelectrolyte, as well as their applications with respect to their response to pH changes during and after assembly.

Brushes of Weak Polyelectrolytes

Polymer brushes are obtained through the chemical grafting of surfaces with a layer of polymers. The resulting coatings are considered more stable than those obtained from casting owing to their capability to withstand immersion in good solvent without dissolution. Polymer brushes are extensively used as colloidal stabilizers, lubricating layers, and drug delivery systems [START_REF] Feng | Polymer Brushes: Efficient Synthesis and Applications[END_REF]. In that respect, elaborating brushes based on weak polyelectrolytes is generally aimed at developing stimuli-responsive systems, and was reviewed recently [START_REF] Geoghegan | Weak Polyelectrolyte Brushes[END_REF]. Grafting-from approaches, where polyelectrolyte chains grow in situ from their surface anchoring points, typically offering higher grafting densities than approaches where pre-synthesized polyelectrolyte chains are used [START_REF] Tomlinson | Creation of Dense Polymer Brush Layers by the Controlled Deposition of an Amphiphilic Responsive Comb Polymer[END_REF][START_REF] Jones | Polymers at Surfaces and Interfaces[END_REF], have therefore been developed by using several radical polymerization technics [START_REF] Feng | Polymer Brushes: Efficient Synthesis and Applications[END_REF]. Accordingly, both polyanion [START_REF] Datsyuk | In Situ Nitroxide-Mediated Polymerized Poly(Acrylic Acid) as a Stabilizer/Compatibilizer Carbon Nanotube/Polymer Composites[END_REF] and polycation [START_REF] Glasing | Grafting Well-Defined CO2-Responsive Polymers to Cellulose Nanocrystals via Nitroxide-Mediated Polymerisation: Effect of Graft Density and Molecular Weight on Dispersion Behaviour[END_REF] brushes have been synthesized by using nitroxide-mediated polymerization (NMP). A larger variety of systems were made accessible using atom-transferradical-polymerization (ATRP) at the cost of using a metal catalyst, yielding routinely weak polycationic brushes such as PDMAEMA [START_REF] Bhat | Assembly of Nanoparticles Using Surface-Grafted Orthogonal Polymer Gradients[END_REF]. However, the growth of carboxylatecontaining polyanions (PAA, PMAA) brushes is more complicated owing to interactions with the catalyst, leading researchers to polymerize their respective ester derivatives, followed by a deprotection step [START_REF] Ryan | Responsive Brushes and Gels as Components of Soft Nanotechnology[END_REF]. Finally, reversible addition-fragmentation chain transfer (RAFT), a catalyst-free polymerization method, was used for developing weak polycationic brushes [START_REF] Wang | Fabrication of Nonfouling, Bactericidal, and Bacteria Corpse Release Multifunctional Surface through Surface-Initiated RAFT Polymerization[END_REF][START_REF] Cho | Polymer Brush Growth by Oxygen-Initiated RAFT Polymerization on Various Substrates[END_REF] with the trade-off of using quite expensive chain transfer agents. However, this approach has so far been neglected with regard to the synthesis of weak polyanion brushes.

The pH response of weak polyelectrolyte brushes leads to conformational changes in polymer chains, resulting in thickness variations in the brush: when the ionization degree of the polyelectrolyte decreases (resp. increases), the brush tends to collapse (resp. swell). This effect is largely modulated by the concentration [START_REF] Zhulina | Theory of Ionizable Polymer Brushes[END_REF], type [START_REF] Zhang | Tuning the PH Response of Weak Polyelectrolyte Brushes with Specific Anion Effects[END_REF], and valency [START_REF] Yu | Multivalent Counterions Diminish the Lubricity of Polyelectrolyte Brushes[END_REF] of counter-ions and salt in the system. Changes in pH and ionic strength parameters can also be induced electrochemically, yielding a reversible swelling of brushes [START_REF] Dunderdale | Coupling PH-Responsive Polymer Brushes to Electricity: Switching Thickness and Creating Waves of Swelling or Collapse[END_REF]. Yet, when designing such pH-responsive systems, one must keep in mind that the apparent pKa (or pKaH) of weak polyelectrolytes in brushes greatly varies with the intrinsic parameters, such as their grafting density [START_REF] Schüwer | Tuning the PH Sensitivity of Poly(Methacrylic Acid) Brushes[END_REF] and ionic strength [START_REF] Del Castillo | Large Changes in Protonation of Weak Polyelectrolyte Brushes with Salt Concentration-Implications for Protein Immobilization[END_REF]. These responses have been exploited to design a range of functional systems, as described in Table 2. Among the most studied applications of polyelectrolyte brushes, both their lubricating and adhesion functions can be efficiently switched on and off by using weak polyelectrolytes [START_REF] Yu | Multivalent Counterions Diminish the Lubricity of Polyelectrolyte Brushes[END_REF][START_REF] Zhang | The Interfacial Behaviour of Single Poly(N,N-Dimethylacrylamide) Chains as a Function of PH[END_REF]. Lubrication is ensured by the swollen hydrated state of polyelectrolyte brushes. Although polyzwitterionic brushes have been determined to perform better, brushes composed of weak polyelectrolytes offer an opportunity to modulate lubrication through pH and ionic (concentration, valency) stimuli [START_REF] Yu | Multivalent Counterions Diminish the Lubricity of Polyelectrolyte Brushes[END_REF]. Adhesion in water dependent on pH has also been demonstrated between a range of weak polyelectrolyte brushes, including PAA with poly(N,N-dimethylacrylamide) and PMAA with PDMAEMA. The adhesion mechanism is, however, different with H-bonding, ensuring the cohesion of the first polyelectrolyte couple (pH values smaller than 2) [START_REF] Sudre | Reversible Adhesion between a Hydrogel and a Polymer Brush[END_REF], while electrostatic interactions dominate for the PMAA/PDMAEMA couple (adhesion for pH values close to 7) [START_REF] Spina | Controlling Network-Brush Interactions to Achieve Switchable Adhesion[END_REF]. The combination of electrostatic, H-bonding, and hydrophobic interactions in weak polyelectrolyte brushes enable cargo (e.g., proteins) immobilization with a large binding capacity, reversible nature, and structure-preserving ability [START_REF] Del Castillo | Enzyme Immobilization in Polyelectrolyte Brushes: High Loading and Enhanced Activity Compared to Monolayers[END_REF][START_REF] Takasu | Polymer Brush Biointerfaces for Highly Sensitive Biosensors That Preserve the Structure and Function of Immobilized Proteins[END_REF]. However, in a recent example, Ferrand-Drake del Castillo et al. suggested that the dominant force for protein loading and release when using weak polyelectrolyte brushes was not electrostatic, owing to the large changes in their apparent pKa [START_REF] Del Castillo | Large Changes in Protonation of Weak Polyelectrolyte Brushes with Salt Concentration-Implications for Protein Immobilization[END_REF].

Electrodeposited Weak Polyelectrolytes Films

Electrochemically induced film deposition strategies have recently attracted a great deal of attention, as the localized nature of the electrochemical trigger enables spatially resolved film assembly in a conformal manner, including on substrates with complex topologies. Applications of such films are anticipated in many fields, as testified by pioneering work in biomaterials, energy storage and conversion, mass transport, and analytic tools [START_REF] Rydzek | Electrochemical Nanoarchitectonics and Layer-by-Layer Assembly: From Basics to Future[END_REF]. Several electrochemical approaches to weak polyelectrolyte films have thus been developed by using electrochemically induced precipitation, electropolymerization, and electrochemically induced coupling reactions (Figure 2). In this section, these different strategies will be described, with a focus on experimental synthesis parameters and applications. Main approaches for electrochemically induced film formatio include electropolymerization, electrochemically induced precipitation, and coupling. The resultin surface coatings containing weak polyelectrolytes combine pH responsiveness (A) with eventua self-organization (B) and combination with inorganic units (C). Figures (A) [START_REF] Rydzek | Morphogen-Driven Self-Construction of Covalent Films Built from Polyelectrolytes and Homobifunctional Spacers: Buildup and PH Response[END_REF], (B) [START_REF] Song | Electrochemical Self-Assembly of Nano-Polyaniline Film by Forced Convection and Its Capacitive Performance[END_REF], and (C [START_REF] Sciortino | Electro-Click Construction of Hybrid Nanocapsule Films with Triggered Delivery Properties[END_REF] are reproduced with permission from the original articles.

The electrochemically induced precipitation of weak polyelectrolyte films is typicall achieved by locally changing the solubility of weak polyelectrolytes near the electrode favoring their self-association and precipitation. This process can be triggered, among th most popular strategies, by changing the local pH through water electrolysis (i) or by en Main approaches for electrochemically induced film formation include electropolymerization, electrochemically induced precipitation, and coupling. The resulting surface coatings containing weak polyelectrolytes combine pH responsiveness (A) with eventual self-organization (B) and combination with inorganic units (C). Figures (A) [START_REF] Rydzek | Morphogen-Driven Self-Construction of Covalent Films Built from Polyelectrolytes and Homobifunctional Spacers: Buildup and PH Response[END_REF], (B) [START_REF] Song | Electrochemical Self-Assembly of Nano-Polyaniline Film by Forced Convection and Its Capacitive Performance[END_REF], and (C) [START_REF] Sciortino | Electro-Click Construction of Hybrid Nanocapsule Films with Triggered Delivery Properties[END_REF] are reproduced with permission from the original articles.

The electrochemically induced precipitation of weak polyelectrolyte films is typically achieved by locally changing the solubility of weak polyelectrolytes near the electrode, favoring their self-association and precipitation. This process can be triggered, among the most popular strategies, by changing the local pH through water electrolysis (i) or by enabling the complexation of the polyelectrolytes with multivalent ions (ii). In this context, electrodeposited weak polyelectrolytes films have mainly been developed from pure and composite assemblies of CHI, ALG, PAH, and gelatin on a large range of electrodes (including patterned electrodes) with spatial and temporal control [START_REF] Zou | Electrodeposition of Polysaccharide and Protein Hydrogels for Biomedical Applications[END_REF].

(i) Water electrolysis allows increasing the local pH value at the cathode, favoring the deposition of films based on weak polycations, such as CHI, through decreasing their ionization degree [START_REF] Zou | Electrodeposition of Polysaccharide and Protein Hydrogels for Biomedical Applications[END_REF]. Here, again, the ionic strength of the solution during the film deposition directly influences the thickness and mechanical properties of the polyelectrolyte assembly, with thicker and softer films obtained at higher salt concentrations [START_REF] Liu | Electrodeposition of a Weak Polyelectrolyte Hydrogel: Remarkable Effects of Salt on Kinetics, Structure and Properties[END_REF]. At the anode, the proton gradient generated during water electrolysis promotes film assembly from weak polyanions such as HA, ALG, and silk fibroin through protonation and selfassociation (Table 3). In contrast, a few examples have reported electrodeposition strategies where an increase in the charge density of weak polyelectrolytes was sought. In that case, proton and hydroxide ion gradients generated by electrolysis in aqueous solutions induce higher ionization degrees and/or acid deprotection of the weak polyelectrolytes, enabling their complexation and film precipitation with oppositely charged polymers [START_REF] Dochter | Film Self-Assembly of Oppositely Charged Macromolecules Triggered by Electrochemistry through a Morphogenic Approach[END_REF][START_REF] Garnier | Surface Confined Self-Assembly of Polyampholytes Generated from Charge-Shifting Polymers[END_REF][START_REF] Sadman | Controlled Electrochemical Deposition of Polyelectrolyte Complex Films[END_REF] and multivalent MoO 4 2-ions [START_REF] Martin | Anodic Electrodeposition of a Cationic Polyelectrolyte in the Presence of Multivalent Anions[END_REF]. (ii) The complexation of weak polyelectrolytes with multivalent ions leading to their film precipitation is another prominent electrodeposition strategy. The generation of the crosslinking ions is achieved either by the dissolution of oxide precursors [START_REF] Liu | Electrical Signals Triggered Controllable Formation of Calcium-Alginate Film for Wound Treatment[END_REF] or by direct oxidation to the relevant cations [START_REF] Rydzek | Electrochemically Triggered Assembly of Films: A One-Pot Morphogen-Driven Buildup[END_REF]. Anodic electrolysis has been used to dissolve CaCO 3 (respectively, Cu 2 (OH) 2 CO 3 ), yielding Ca 2+ and Cu 2+ ions near the electrode and allowing the assembly of ALG films by complexation [START_REF] Liu | Electrical Signals Triggered Controllable Formation of Calcium-Alginate Film for Wound Treatment[END_REF][START_REF] Wang | Continuous and Controllable Electro-Fabrication of Antimicrobial Copper-Alginate Dressing for Infected Wounds Treatment[END_REF]. Allowing work to be carried out at milder oxidation potentials, the electrochemical generation of multivalent cations has been developed with Cu 2+ , Fe 3+ , and Ru 2+ cations, allowing complexation and film deposition with ALG, Chitin, CHI, and PAA modified with terpyridine groups [START_REF] Wang | Continuous and Controllable Electro-Fabrication of Antimicrobial Copper-Alginate Dressing for Infected Wounds Treatment[END_REF][START_REF] Dwivedi | Dye-Sensitized Solar Cells with Polyaniline: A Review[END_REF][START_REF] Kumar | Polypyrrole and Polyaniline-Based Membranes for Fuel Cell Devices: A Review[END_REF].

In situ polymerization on electrodes by electrochemical reaction (electropolymerization) is another popular strategy that can ensure the elaboration of functional films. Aniline, dopamine, carbazole, and vinylpyridine monomers and their derivatives have been extensively studied for use in the electropolymerization of weak polyelectrolyte films under oxidative potentials (Table 3). The resulting films can combine electron conductivity with weak polybase characteristics, conferring them with doping, complexation, and molecular imprinting abilities. A large number of recent studies have used these appealing properties to design sensors, energy devices, light-emitting devices, and electrochromic and "smart" electrodes, as described in recent reviews [START_REF] Dwivedi | Dye-Sensitized Solar Cells with Polyaniline: A Review[END_REF][START_REF] Kumar | Polypyrrole and Polyaniline-Based Membranes for Fuel Cell Devices: A Review[END_REF][START_REF] Holze | Conjugated Molecules and Polymers in Secondary Batteries: A Perspective[END_REF][START_REF] Ramanavicius | Electrochemical Molecularly Imprinted Polymer Based Sensors for Pharmaceutical and Biomedical Applications (Review)[END_REF][START_REF] Bekkar | Polycarbazole and Its Derivatives: Synthesis and Applications. A Review of the Last 10 Years[END_REF]. The presence of aromatic cycles in such polymers also confers them with a hydrophobic character and self-assembling properties that enable them not only to act as weak polyelectrolytes but also as structure directing nano-units (Figure 2B). For instance, PANI self-organization has been exploited to design nanostructured colloids [START_REF] Sanchez-Ballester | Nanostructured Polymeric Yolk-Shell Capsules: A Versatile Tool for Hierarchical Nanocatalyst Design[END_REF] and coatings [START_REF] Song | Electrochemical Self-Assembly of Nano-Polyaniline Film by Forced Convection and Its Capacitive Performance[END_REF][START_REF] Rydzek | Simultaneous Electropolymerization and Electro-Click Functionalization for Highly Versatile Surface Platforms[END_REF], which are optimized as catalytic supports and energy storage platforms, respectively. Accordingly, several self-organized structured polyvinylpyrridine sensors have been reported [START_REF] Behzadi Pour | Polyvinylpyridine-Based Electrodes: Sensors and Electrochemical Applications[END_REF]. In the case of polydopamine, the presence of functional groups such as catechol, amine, and imine in its molecular structure makes it a prime candidate for use in biomedical applications [START_REF] Ball | Physicochemical Perspective on "Polydopamine" and "Poly(Catecholamine)" Films for Their Applications in Biomaterial Coatings (Review)[END_REF] with the ability to self-organize into microstructured films [START_REF] Lin | Micro-Structured Polydopamine Films via Pulsed Electrochemical Deposition[END_REF].

Contrary to electropolymerization, electro-coupling relies on non-propagative covalent couplings induced electrochemically between functional nano-units bearing adequate moieties. To date, the alkyne-azide "click" cycloaddition (i) and the dimerization of 9-alkylcarbazoles (ii) are the most studied systems enabling the electrosynthesis of polymer and hybrid films [START_REF] Rydzek | Electrochemically Triggered Assembly of Films: A One-Pot Morphogen-Driven Buildup[END_REF][START_REF] Li | Electrochemical-Coupling Layer-by-Layer (ECC-LbL) Assembly[END_REF].

(i) The alkyne-azide "click" cycloaddition, specifically leading to covalent triazole bonds under mild and aqueous conditions, is typically catalyzed by Cu(I) ions. The generation of these ions, which are unstable in water, by the electrochemical reduction of Cu(II) ions (using -0.3 V vs. Ag/AgCl), induces and confines the "click" coupling reaction in the vicinity of electrodes [START_REF] Rydzek | Electrochemically Triggered Assembly of Films: A One-Pot Morphogen-Driven Buildup[END_REF]. This film assembling strategy has been carried out with various weak polyelectrolytes (PAA, PAH) grafted with alkyne/azide groups, yielding covalently reticulated single-polyelectrolyte coatings that reversibly swell in response to post-assembly pH changes [START_REF] Rydzek | Electrochemically Triggered Assembly of Films: A One-Pot Morphogen-Driven Buildup[END_REF]. Accordingly, the covalent incorporation of fluorescent bis-pyrene moieties in such coatings composed of PAA evidenced the buffer behavior of the film, which maintained its internal pH at a value close to 3.5, while the environmental pH was changed from pH 4 to 7 [START_REF] Rydzek | Morphogen-Driven Self-Construction of Covalent Films Built from Polyelectrolytes and Homobifunctional Spacers: Buildup and PH Response[END_REF]. In most recent examples, this click electrosynthesis approach has been combined with the electropolymerization of PANI [START_REF] Rydzek | Simultaneous Electropolymerization and Electro-Click Functionalization for Highly Versatile Surface Platforms[END_REF] and adapted to hybrid nano-units, leading to pH-responsive nanostructured coatings with cargo encapsulation/release abilities (Figure 2C) [START_REF] Sciortino | Electro-Click Construction of Hybrid Nanocapsule Films with Triggered Delivery Properties[END_REF].

(ii) Various organic and inorganic nano-units have been substituted in the 9-position of 9-alkylcarbazoles, enabling their covalent coupling by the electrochemical dimerization of carbazole moieties in acetonitrile [START_REF] Rydzek | Electrochemical Nanoarchitectonics and Layer-by-Layer Assembly: From Basics to Future[END_REF]. This unique behavior was attributed to the selective activation of 3-and 6-positions of carbazoles and exhibited sensitivity to the potential applied: +1.0 V (vs. Ag/AgCl) favored more dimerization while +1.2 V also led to oligomerization [START_REF] Li | C3-C3 and C6-C6 Oxidative Couplings of Carbazoles[END_REF]. The obtained organic or hybrid films benefited from internal layered structures and unlocking optical limiting applications [START_REF] Du | Tuning Optical Limiting of Heterosized AuNPs and Fullerene by Countable Electrochemical Assembly[END_REF][START_REF] Kang | Electrochemically Organized Isolated Fullerene-Rich Thin Films with Optical Limiting Properties[END_REF]. Following this concept, recent efforts to potentialize other electrochemical dimerization reactions for functional films have been reported, opening promising perspectives for multifunctional systems [START_REF] Wang | Controlled Electropolymerization Based on Self-Dimerizations of Monomers[END_REF]. pH sensors, triggered release [START_REF] Rydzek | Morphogen-Driven Self-Construction of Covalent Films Built from Polyelectrolytes and Homobifunctional Spacers: Buildup and PH Response[END_REF][START_REF] Rydzek | Electrochemically Triggered Assembly of Films: A One-Pot Morphogen-Driven Buildup[END_REF] Controlled dimerization Dimerization of alkylcarbazoles (+1.0 V/+1.2 V, acetonitrile) Photovoltaics [START_REF] Gu | In Situ Electrochemical Deposition and Doping of C60 Films Applied to High-Performance Inverted Organic Photovoltaics[END_REF] Electropolymerization PANI (1.0 M HNO 3 aqueous, 2 mA cm -2 ) PANI (0.5 M H 2 SO 4 aqueous, CV -0.6 V/+1.5 V) Polycarbazoles (+1.3 V, aqueous or acetonitrile) Polydopamine (0.1 M phosphate buffer saline, CV -0.5 V/+0.5 V)

Capacitors, Capacitor sensors, Opto-electronic and electrochemical Sensors, biocoatings [START_REF] Song | Electrochemical Self-Assembly of Nano-Polyaniline Film by Forced Convection and Its Capacitive Performance[END_REF][START_REF] Rydzek | Simultaneous Electropolymerization and Electro-Click Functionalization for Highly Versatile Surface Platforms[END_REF] [76] [START_REF] Kund | Physicochemical and Electrochemical Characterization of Electropolymerized Polydopamine Films: Influence of the Deposition Process[END_REF] Electrochemically induced precipitation None reported [START_REF] Dochter | Film Self-Assembly of Oppositely Charged Macromolecules Triggered by Electrochemistry through a Morphogenic Approach[END_REF][START_REF] Garnier | Surface Confined Self-Assembly of Polyampholytes Generated from Charge-Shifting Polymers[END_REF][START_REF] Sadman | Controlled Electrochemical Deposition of Polyelectrolyte Complex Films[END_REF]

Chitin (+1.2 V with Fe 2+ ions) CHI (+1.5 V with Cu(s)) CHI (+1 to +3 V) Collagen (pH 3.5; 0.1 M H 2 O 2 ,

Layer-by-Layer Films and Vectors from Weak Polyelectrolytes

Over the past few decades, the Layer-by-Layer (LbL) strategy has enabled adsorbed polyelectrolyte films with unprecedented functional versatility. This approach, pioneered by Iler in 1966 and developed by Decher from 1991 [START_REF] Decher | Fuzzy Nanoassemblies: Toward Layered Polymeric Multicomposites[END_REF], relies on the sequential adsorption of complementary chemical species (including polyelectrolytes), yielding coatings with controllable thickness whose growth can be driven by a range of interactions, including electrostatics, H-bonding, molecular recognition, and coordination (Figure 3) [START_REF] Yuan | Weak Polyelectrolyte-Based Multilayers via Layer-by-Layer Assembly: Approaches, Properties, and Applications[END_REF]. LbL films have thus attracted attention relating to their use in Nanoarchitectonics, owing to their potential for assembling functional nano-units on a large variety of substrates and topologies [START_REF] Ariga | Layer-by-Layer Nanoarchitectonics: Invention, Innovation, and Evolution[END_REF]. In this context, weak polyelectrolytes are mostly positioned as building blocks granting reversible interaction abilities to multilayers. [START_REF] Decher | Fuzzy Nanoassemblies: Toward Layered Polymeric Multicomposites[END_REF] and (B) [START_REF] Bataglioli | Interplay of the Assembly Conditions on Drug Transport Mechanisms in Polyelectrolyte Multilayer Films[END_REF] are reproduced with permission from the origin

Layer by Layer Films

Weak polyelectrolytes of both synthetic and natural origin have bee in LbL films, influencing their structure and composition [START_REF] Iqbal | Effect of the Buffer on the Buildup and Stability of Tannic Acid/Collagen Multilayer Films Applied as Antibacterial Coatings[END_REF], as well as th stimuli. Such films have thus been readily employed as biointerfaces and tunable mass transport properties [START_REF] Yuan | Weak Polyelectrolyte-Based Multilayers via Layer-by-Layer Assembly: Approaches, Properties, and Applications[END_REF] (as described in Table 4). Accordi the charge density and conformation of weak polyelectrolytes via pH and ters during (i) and after (ii) assembling multilayer films has been exploited

(i) Decreasing the charge density of weak polyelectrolytes, either by d ionization degree of by increasing charge screening by salts, typically fav formations (Scheme 1). In the case of LbL film buildups relying on electros interactions, assembly conditions with smaller polyelectrolyte charge den less crosslinks and more loops between the layers, leading to larger thickn at each adsorption step of weak polyelectrolytes compared to condition tended conformation of the polyelectrolyte is favored (corresponding to density). Accordingly, the growth mechanism of a polydiallyldimethylam ride (PDADMAC)/ALG multilayer changed from linear to exponential beh assembly pH value was changed from pH 10 (where both polyelectrolytes pH 3 (where ALG is protonated) [START_REF] Dos Santos De Macedo | Effect of PH on the Complex Coacervation and on the Formation of Layers of Sodium Alginate and PDADMAC[END_REF]. It follows that the multilayer thick Figures (A) [START_REF] Decher | Fuzzy Nanoassemblies: Toward Layered Polymeric Multicomposites[END_REF] and (B) [START_REF] Bataglioli | Interplay of the Assembly Conditions on Drug Transport Mechanisms in Polyelectrolyte Multilayer Films[END_REF] are reproduced with permission from the original articles.

Layer by Layer Films

Weak polyelectrolytes of both synthetic and natural origin have been incorporated in LbL films, influencing their structure and composition [START_REF] Iqbal | Effect of the Buffer on the Buildup and Stability of Tannic Acid/Collagen Multilayer Films Applied as Antibacterial Coatings[END_REF], as well as their response to stimuli. Such films have thus been readily employed as biointerfaces and platforms with tunable mass transport properties [START_REF] Yuan | Weak Polyelectrolyte-Based Multilayers via Layer-by-Layer Assembly: Approaches, Properties, and Applications[END_REF] (as described in Table 4). Accordingly, adjusting the charge density and conformation of weak polyelectrolytes via pH and ionic parameters during (i) and after (ii) assembling multilayer films has been exploited:

(i) Decreasing the charge density of weak polyelectrolytes, either by decreasing their ionization degree of by increasing charge screening by salts, typically favors coiled conformations (Scheme 1). In the case of LbL film buildups relying on electrostatically driven interactions, assembly conditions with smaller polyelectrolyte charge densities result in less crosslinks and more loops between the layers, leading to larger thickness increments at each adsorption step of weak polyelectrolytes compared to conditions where an extended conformation of the polyelectrolyte is favored (corresponding to higher charge density). Accordingly, the growth mechanism of a polydiallyldimethylammonium chloride (PDADMAC)/ALG multilayer changed from linear to exponential behavior when the assembly pH value was changed from pH 10 (where both polyelectrolytes are ionized) to pH 3 (where ALG is protonated) [START_REF] Dos Santos De Macedo | Effect of PH on the Complex Coacervation and on the Formation of Layers of Sodium Alginate and PDADMAC[END_REF]. It follows that the multilayer thickness, chemical composition, mechanical properties, permeability, and adhesion are largely determined by pH and ionic parameters during the LbL deposition process. Therefore, the swelling of PAH/PAA and PLL/HA multilayers in aqueous media increases when the charge density of their weak polyelectrolytes decreases, a behavior attributed to the reduced ionic crosslink density and to loopy chain conformations [START_REF] Tanchak | Swelling Dynamics of Multilayer Films of Weak Polyelectrolytes[END_REF][START_REF] Bütergerds | PH-Dependent Growth Laws and Viscoelastic Parameters of Poly-l-Lysine/Hyaluronic Acid Multilayers[END_REF]. The same phenomenon controls the hardness and elastic modulus of PAH/PAA multilayers [100], leading to lower values being obtained for PAH(pH 7.5)/PAA(pH 3.5) when both polyelectrolytes have a smaller charge density than for PAH(pH 6.5)/PAA(pH 6.5). Consequently, the assembly pH has been demonstrated to influence the drug transport mechanism in PAH/PAA multilayer films (Figure 3) [START_REF] Bataglioli | Interplay of the Assembly Conditions on Drug Transport Mechanisms in Polyelectrolyte Multilayer Films[END_REF]. In contrast, when the LbL film buildup relies on H-bonding, increasing the ionization rate of weak polyelectrolytes follows the opposite trend: for instance, the buildup of PAA/poly(vinylpyrrolidone) multilayers was hindered at pH values where PAA had a high charge density [START_REF] Yuan | Weak Polyelectrolyte-Based Multilayers via Layer-by-Layer Assembly: Approaches, Properties, and Applications[END_REF]101].

(ii) Post-assembly changes in pH and ionic parameters have been extensively studied as external stimuli to weak polyelectrolyte multilayers with respect to their response in thickness, porous structure, and encapsulation/release changes [START_REF] Yuan | Weak Polyelectrolyte-Based Multilayers via Layer-by-Layer Assembly: Approaches, Properties, and Applications[END_REF]102]. For LbL films relying on electrostatic interactions, decreasing the charge density of weak polyelectrolytes, either by post-assembly pH changes or by increasing the solution ionic strength, resulted in the swelling of the films [103] and eventually their dissolution. Accordingly, swelling coefficients of, respectively, up to 5-fold and 8-fold were found for PLL/HA multilayers subjected to ionic strength [104] and pH [105] stimuli. The swelling process was accompanied by structural changes in multilayer films, such as the emergence of holes in PLL/HA films when the NaCl concentration was changed from 0.15 M to 0.48 M [105] and the growth of pores in (PEI/PAH)/PAA films after the post-assembly pH was decreased to pH 2 [106]. The potential of such porous coatings was tested for designing slippery liquid-infused porous surfaces [106,107]. Concurrently, the modulation of the mass transport properties of weak polyelectrolyte multilayer films by post-assembly treatment has attracted much attention [108], leading to systems with tailored functions (Table 4), including ionic current rectification [109], enhanced Li + conductivity [110], and filtration [111]. Conversely, not only the ionic strength but also the nature and valence of the salt used as the post-assembly stimulus were found to modulate the properties of weak polyelectrolyte multilayers: exposing a PAH/PAA multilayer film to various concentrations and type of metal ions enabled changing their pore sizes from 54 nm to 1.63 µm. This behavior was ascribed to phase separation in the film induced by metal-ion coordination with PAH [112], and was used to trap silver ions in the film to selectively detect methylmercaptan gas concentrations as low as 20 ppb [113].

Colloidal Systems Based on LbL Multilayers of Weak Polyelectrolytes

The LbL film deposition process has been adapted to colloidal substrates, providing an alternative to polymer self-assembly for designing nanocarriers, including hollow capsules (Figure 4) [121]. This method requires a sacrificial template (such as melamine formaldehyde, polystyrene, poly(methacrylic acid), silica, calcium carbonates, hydrogel microspheres), which needs to be removed by calcination or etching, and can be used to develop biocompatible systems with encapsulated drugs. Nevertheless, such nanocarriers remain very efficient when applied to catalysis [122] With their high degree of functionality as well as their versatility, weak polyelectrolytes have been widely used to develop new tools for precision medicine in order to simultaneously address precision diagnosis and precision therapy. In this context, the use of polymer nanocarriers offers three topological regions which can allow functionalization: the inner cavity, the surrounding shell, and the external surface exposed to the microenvironment (Figure 4). Polyelectrolytes have consequently led to the development of nanocarriers for applications in imaging and for therapeutic purposes (Table 5). The ondemand release of cargo can be achieved by external or endogenous stimuli and provide a variety of ways to control the dosage, time, and location of release [132]. Temperature stands as a prominent stimulus, allowing changes in the hydration degree or layer organization which can be exploited for permeability changes and the release of the carrier [126]. Thermal responses can also be achieved by the use of inorganic nanoparticles such as gold [133], silver [134], and nanodiamond [135]. Under light irradiation, the local heating of nanoparticles can induce the rupture of the polymer shell or the modification of its permeability. This has been particularly demonstrated with polyelectrolytes such as PAH and poly(styrenesulfonate)(PSS) embedded with gold nanoparticles. This study demonstrated that, upon local heating by the gold nanoparticle, cargos retain their biological activities, although their diffusion within the cell is slightly decreased [136]. Ultrasounds can also be exploited as external stimuli to control the rupture of polyelectrolyte capsules. With their high degree of functionality as well as their versatility, weak polyelectrolytes have been widely used to develop new tools for precision medicine in order to simultaneously address precision diagnosis and precision therapy. In this context, the use of polymer nanocarriers offers three topological regions which can allow functionalization: the inner cavity, the surrounding shell, and the external surface exposed to the microenvironment (Figure 4). Polyelectrolytes have consequently led to the development of nanocarriers for applications in imaging and for therapeutic purposes (Table 5). The on-demand release of cargo can be achieved by external or endogenous stimuli and provide a variety of ways to control the dosage, time, and location of release [132]. Temperature stands as a prominent stimulus, allowing changes in the hydration degree or layer organization which can be exploited for permeability changes and the release of the carrier [126]. Thermal responses can also be achieved by the use of inorganic nanoparticles such as gold [133], silver [134], and nanodiamond [135]. Under light irradiation, the local heating of nanoparticles can induce the rupture of the polymer shell or the modification of its permeability. This has been particularly demonstrated with polyelectrolytes such as PAH and poly(styrenesulfonate)(PSS) embedded with gold nanoparticles. This study demonstrated that, upon local heating by the gold nanoparticle, cargos retain their biological activities, although their diffusion within the cell is slightly decreased [136]. Ultrasounds can also be exploited as external stimuli to control the rupture of polyelectrolyte capsules. Ultrasounds are commonly used in imaging and ablative therapy. In the latter case, the mechanical deformation induced causes the bursting of the capsules, thus releasing their payload [137]. Magnetic fields have also been used to trigger the release of polymer capsules [132]. With the combination of magnetic nanoparticles such as iron oxide and electromagnetic fields of various frequency and power, different mechanisms can be induced. At low frequencies, a non-heating process can preferably be used to preserve tissues as well as a bioactive payload such as enzymes or DNA [138]. Meanwhile, with higher-frequency magnetic fields, a high increase in the local temperature induces the destruction of cellular and subcellular structures [139].

Gels and Vectors Based on Weak Polyelectrolytes Complexes

Processing weak polyelectrolyte into gels and vectors has been a very active research field for the past few decades. A range of approaches yielding prominently biosourced single-polyelectrolyte gels have been proposed based on precipitation/coagulation through H-bonding, hydrophobic interactions, and crosslinking through reactions with

Gels and Vectors Based on Weak Polyelectrolytes Complexes

Processing weak polyelectrolyte into gels and vectors has been a very active research field for the past few decades. A range of approaches yielding prominently biosourced single-polyelectrolyte gels have been proposed based on precipitation/coagulation through H-bonding, hydrophobic interactions, and crosslinking through reactions with either ionic or covalent crosslinkers [152,153]. Although this research will not be covered

Gels and Vectors Based on Weak Polyelectrolytes Complexes

Processing weak polyelectrolyte into gels and vectors has been a very active research field for the past few decades. A range of approaches yielding prominently biosourced single-polyelectrolyte gels have been proposed based on precipitation/coagulation through H-bonding, hydrophobic interactions, and crosslinking through reactions with either ionic or covalent crosslinkers [152,153]. Although this research will not be covered in this review, recently published works are highlighted for further reading on this topic [154][155][156]. In contrast, processing polyelectrolyte complexes formed from the spontaneous

Gels and Vectors Based on Weak Polyelectrolytes Complexes

Processing weak polyelectrolyte into gels and vectors has been a very active research field for the past few decades. A range of approaches yielding prominently biosourced single-polyelectrolyte gels have been proposed based on precipitation/coagulation through H-bonding, hydrophobic interactions, and crosslinking through reactions with either ionic or covalent crosslinkers [152,153]. Although this research will not be covered in this review, recently published works are highlighted for further reading on this topic [154][155][156]. In contrast, processing polyelectrolyte complexes formed from the spontaneous entropy-driven complexation of polyanions and polycations [START_REF] Fu | Driving Forces for Oppositely Charged Polyion Association in Aqueous Solutions: Enthalpic, Entropic, but Not Electrostatic[END_REF] into gels and membranes

Gels and Vectors Based on Weak Polyelectrolytes Complexes

Processing weak polyelectrolyte into gels and vectors has been a very active research field for the past few decades. A range of approaches yielding prominently biosourced single-polyelectrolyte gels have been proposed based on precipitation/coagulation through H-bonding, hydrophobic interactions, and crosslinking through reactions with either ionic or covalent crosslinkers [152,153]. Although this research will not be covered in this review, recently published works are highlighted for further reading on this topic [154][155][156]. In contrast, processing polyelectrolyte complexes formed from the spontaneous entropy-driven complexation of polyanions and polycations [START_REF] Fu | Driving Forces for Oppositely Charged Polyion Association in Aqueous Solutions: Enthalpic, Entropic, but Not Electrostatic[END_REF] into gels and membranes is a method that has only been developed recently [START_REF] Durmaz | Weak Polyanion and Strong Polycation Complex Based Membranes: Linking Aqueous Phase Separation to Traditional Membrane Fabrication[END_REF]157]. This section will therefore

Gels and Vectors Based on Weak Polyelectrolytes Complexes

Processing weak polyelectrolyte into gels and vectors has been a very active research field for the past few decades. A range of approaches yielding prominently biosourced single-polyelectrolyte gels have been proposed based on precipitation/coagulation through H-bonding, hydrophobic interactions, and crosslinking through reactions with either ionic or covalent crosslinkers [152,153]. Although this research will not be covered in this review, recently published works are highlighted for further reading on this topic [154][155][156]. In contrast, processing polyelectrolyte complexes formed from the spontaneous entropy-driven complexation of polyanions and polycations [START_REF] Fu | Driving Forces for Oppositely Charged Polyion Association in Aqueous Solutions: Enthalpic, Entropic, but Not Electrostatic[END_REF] into gels and membranes is a method that has only been developed recently [START_REF] Durmaz | Weak Polyanion and Strong Polycation Complex Based Membranes: Linking Aqueous Phase Separation to Traditional Membrane Fabrication[END_REF]157]. This section will therefore

Gels and Vectors Based on Weak Polyelectrolytes Complexes

Processing weak polyelectrolyte into gels and vectors has been a very active research field for the past few decades. A range of approaches yielding prominently biosourced single-polyelectrolyte gels have been proposed based on precipitation/coagulation through H-bonding, hydrophobic interactions, and crosslinking through reactions with either ionic or covalent crosslinkers [152,153]. Although this research will not be covered in this review, recently published works are highlighted for further reading on this topic [154][155][156]. In contrast, processing polyelectrolyte complexes formed from the spontaneous entropy-driven complexation of polyanions and polycations [START_REF] Fu | Driving Forces for Oppositely Charged Polyion Association in Aqueous Solutions: Enthalpic, Entropic, but Not Electrostatic[END_REF] into gels and membranes is a method that has only been developed recently [START_REF] Durmaz | Weak Polyanion and Strong Polycation Complex Based Membranes: Linking Aqueous Phase Separation to Traditional Membrane Fabrication[END_REF]157]. This section will therefore address the existing approaches for processing weak polyelectrolyte complexes into gels and membranes before describing selected systems that are relevant for pharmaceutical vectorization.

Gels Based on Weak Polyelectrolyte Complexes

Polyelectrolyte complexation by the electrostatic association of oppositely charged polymers results in phase separation from the solution through the formation of either solid precipitates or liquid complex coacervates. Although fundamental studies to elucidate the exact nature and behavior of these different complexes are still ongoing [START_REF] Neitzel | Expanding the Structural Diversity of Polyelectrolyte Complexes and Polyzwitterions[END_REF][START_REF] Yang | Precision Doping of Polyelectrolyte Complexes: Insight on the Role of Ions[END_REF][START_REF] Digby | Salt Resistance as a Measure of the Strength of Polyelectrolyte Complexation[END_REF], many recent studies have focused on using polyelectrolyte complexes for applied materials, including vectors (described in Section 4.2) and gels. Adjusting the density of electrostatic crosslinks in polyelectrolyte complexes is critical to ensure their material processability. In that context, processable gels made of PDADMAC/PMAA complexes have been developed by screening polyelectrolytes' charge densities with high-ionic-strength solutions (e.g., 2.5 M NaCl) followed by compaction through ultracentrifugation [START_REF] Porcel | Compact Polyelectrolyte Complexes: "Saloplastic" Candidates for Biomaterials[END_REF]. The obtained gels are named "saloplastics" or "compacted complexes of polyelectrolytes (COPEC)" and correspond to the blending of polyelectrolyte chains at the molecular level, where charges are reversibly compensated either intrinsically between polyelectrolytes or extrinsically with counterions (Figure 5). This gel elaboration approach has been successfully applied to several other weak polyelectrolytes, including PAA, ALG, PAH, and CHI, yielding self-healing gels with applications as biomaterials and catalyst supports (Table 6). The compaction process by ultracentrifugation initially represented a bottleneck for the larger-scale production of COPECs, triggering the development of alternative synthesis approaches based on simple centrifugation [158], injection [159], and sedimentation [160]. The properties of the resulting saloplastics vary greatly with the charge density and balance of their polyelectrolyte components both during and after synthesis, typically adjusted by pH and ionic force parameters. It follows that COPECs containing weak polyelectrolytes are dynamic stimuli-responsive materials that enable adjusting a large range of properties, including mechanical properties, composition, porosity, and sorption/release ability (Figure 5). Conversely, we reported on a dramatic increase in the porosity of PAH/PMAA COPECS following Na + to Cu 2+ cation exchange by complexation in the gel, enabling the in situ synthesis of catalytic nanoparticles [START_REF] Sciortino | Saloplastics as Multiresponsive Ion Exchange Reservoirs and Catalyst Supports[END_REF]. The incorporation of biocompatible weak polyelectrolytes (e.g., CHI, ALG) and their cyclodextrin-grafted derivatives has attracted a great deal of attention in the field of biomaterial design [161][162][163].

Molecules 2022, 27, x FOR PEER REVIEW 13 of 32

developed by screening polyelectrolytes' charge densities with high-ionic-strength solutions (e.g., 2.5 M NaCl) followed by compaction through ultracentrifugation [START_REF] Porcel | Compact Polyelectrolyte Complexes: "Saloplastic" Candidates for Biomaterials[END_REF]. The obtained gels are named "saloplastics" or "compacted complexes of polyelectrolytes (COPEC)" and correspond to the blending of polyelectrolyte chains at the molecular level, where charges are reversibly compensated either intrinsically between polyelectrolytes or extrinsically with counterions (Figure 5). This gel elaboration approach has been successfully applied to several other weak polyelectrolytes, including PAA, ALG, PAH, and CHI, yielding self-healing gels with applications as biomaterials and catalyst supports (Table 6). The compaction process by ultracentrifugation initially represented a bottleneck for the larger-scale production of COPECs, triggering the development of alternative synthesis approaches based on simple centrifugation [158], injection [159], and sedimentation [160]. The properties of the resulting saloplastics vary greatly with the charge density and balance of their polyelectrolyte components both during and after synthesis, typically adjusted by pH and ionic force parameters. It follows that COPECs containing weak polyelectrolytes are dynamic stimuli-responsive materials that enable adjusting a large range of properties, including mechanical properties, composition, porosity, and sorption/release ability (Figure 5). Conversely, we reported on a dramatic increase in the porosity of PAH/PMAA COPECS following Na + to Cu 2+ cation exchange by complexation in the gel, enabling the in situ synthesis of catalytic nanoparticles [START_REF] Sciortino | Saloplastics as Multiresponsive Ion Exchange Reservoirs and Catalyst Supports[END_REF]. The incorporation of biocompatible weak polyelectrolytes (e.g., CHI, ALG) and their cyclodextrin-grafted derivatives has attracted a great deal of attention in the field of biomaterial design [161][162][163]. Polyelectrolyte complexes have also been processed into functional membranes by aqueous phase separation [START_REF] Durmaz | Weak Polyanion and Strong Polycation Complex Based Membranes: Linking Aqueous Phase Separation to Traditional Membrane Fabrication[END_REF]. Briefly, polyelectrolytes are first blended at the molecular level in an aqueous solution where the pH or ionic strength values do not allow their complexation, and the subsequent change in that parameter enables the precipitation of polyelectrolyte complex membranes [START_REF] Durmaz | Weak Polyanion and Strong Polycation Complex Based Membranes: Linking Aqueous Phase Separation to Traditional Membrane Fabrication[END_REF]. This approach provides control over membrane pore size and structure in ways analogous to traditional non-solvent-induced phase separation. The synthesis pathway based on pH stimulus was developed with mixtures of weak and strong polyelectrolytes, yielding membranes made from PEI/poly(styrenesulfonate)(PSS), PAH/PSS, and PDADMAC/PAA systems for use in nanofiltration and micropollutant removal [START_REF] Durmaz | Weak Polyanion and Strong Polycation Complex Based Membranes: Linking Aqueous Phase Separation to Traditional Membrane Fabrication[END_REF]165,166].

Weak Polyelectrolyte Complexes for Pharmaceutical Vectorization

The emergence of new diseases and strains of micro-organisms requires constant evolution and research in the field of drug delivery and nanomedicine. The most straightforward strategy adopted by the pharmaceutical industry has been the synthesis of new drugs capable of combating these pathologies. This, in turn, triggers the demand for appropriate drug carriers that can be effectively loaded with these drugs and protect them until they are administered and delivered. Although great progress has been made in the development of new drug carriers, including weak polyelectrolyte complex systems, in recent years [167][168][169], there are still systems such as water-insoluble drugs that these strategies fail to encapsulate efficiently [170].

Polyelectrolytes used for pharmaceutical research must meet the requirements of biocompatible polymer systems and be suitable for use as carriers of active substances. In this sense, the use of weak biosourced polyelectrolytes such as chitosan or charged chitosan derivatives such as glycol-chitosan or N-dodecylated chitosan as polycations and natural polysaccharides such as alginate, pectin, or carrageenan as polyanions has received much attention in the design of polyelectrolyte complexes for drug delivery due to their excellent bioavailability and biodegradability [171][172][173]. In addition to the difficulty of efficiently encapsulating actives with poor water solubility, other challenges in drug delivery include (i) the development of drug delivery systems that provide the sustained release of the drug within a desired therapeutic window to ensure efficacy; (ii) non-specificity, toxicity, and lack of localized administration strategies for certain treatments such as chemotherapeutics; (iii) scalability; and (iv) the development of harmonized regulatory guidelines for the manufacture of nanotechnology products that require contact with the human body (Figure 6). All the challenges cited above make the design of drug delivery systems much more complex than that of non-biological material release. As a result, the construction of efficient drug delivery carriers is usually achieved by assembling several components, each with its own role in the unified delivery function. An interesting way to achieve this is to use nanoarchitectonics approaches to develop biocompatible weak polyelectrolyte complexes formed in water with stimuli-responsive properties. The charges on weak polyelectrolytes are dynamic, causing polymer chains to adopt different equilibrium conformations even with relatively small changes to the surrounding environment [174] (Schemes 1 and 2). For instance, phosphonium polymer has been demonstrated to be able to control the physical and biological properties of sodium hyaluronate/phosphonium polyelectrolyte complexes [175]. The network swelling and therefore drug release rates of these systems can be controlled by varying the concentration of salt in the medium. Thus, while more hydrophilic molecules such as adenosine-5 -triphosphate can be released over 1-2 days, the sustained release of fluorescein and diclofenac over 60 days can be achieved, which is much longer than that previously reported for polyelectrolyte complexes [176][177][178]. On the other hand, only phosphonium polymers, including phenyl substituents, have shown a low cytotoxicity. Another example of improved control of drug release is in chitosan/alginate biocompatible pH-responsive polyelectrolyte complexes, which were developed as less invasive delivery systems for oral insulin administration [179]. This association of polyelectrolytes allows the delivery system to withstand prolonged contact with acidic gastric media and enzymes in the gastrointestinal tract, enhancing bioavailability by controlling insulin release in the intestinal tract. Furthermore, the examined polyelectrolyte complexes exhibit non-cytotoxicity against Caco2 cells.

Molecules 2022, 27, x FOR PEER REVIEW salt in the medium. Thus, while more hydrophilic molecules such as adenosine-5′phate can be released over 1-2 days, the sustained release of fluorescein and di over 60 days can be achieved, which is much longer than that previously repo polyelectrolyte complexes [176][177][178]. On the other hand, only phosphonium po including phenyl substituents, have shown a low cytotoxicity. Another exampl proved control of drug release is in chitosan/alginate biocompatible pH-responsi electrolyte complexes, which were developed as less invasive delivery systems insulin administration [179]. This association of polyelectrolytes allows the deliv tem to withstand prolonged contact with acidic gastric media and enzymes in the intestinal tract, enhancing bioavailability by controlling insulin release in the in tract. Furthermore, the examined polyelectrolyte complexes exhibit non-cyto against Caco2 cells. Molecules 2022, 27, x FOR PEER REVIEW salt in the medium. Thus, while more hydrophilic molecules such as adenosine-5′-tri phate can be released over 1-2 days, the sustained release of fluorescein and diclo over 60 days can be achieved, which is much longer than that previously reporte polyelectrolyte complexes [176][177][178]. On the other hand, only phosphonium poly including phenyl substituents, have shown a low cytotoxicity. Another example o proved control of drug release is in chitosan/alginate biocompatible pH-responsive electrolyte complexes, which were developed as less invasive delivery systems fo insulin administration [179]. This association of polyelectrolytes allows the deliver tem to withstand prolonged contact with acidic gastric media and enzymes in the g intestinal tract, enhancing bioavailability by controlling insulin release in the inte tract. Furthermore, the examined polyelectrolyte complexes exhibit non-cytoto against Caco2 cells. Nanoarchitectonics approaches to develop drug reservoirs with collective nan tem functionality have been used to address the clinical limitations of premature release and tumor non-specificity. For instance, a novel superparamagnetic chit Nanoarchitectonics approaches to develop drug reservoirs with collective nanosystem functionality have been used to address the clinical limitations of premature drug release and tumor non-specificity. For instance, a novel superparamagnetic chitosan-based nanometer-sized colloidal polyelectrolyte complex integrating the water-soluble polymeric prodrug poly(L-glutamic acid)-SN-38 (PGA-SN-38) was designed using a one-shot manufacturing process to efficiently deliver . The combination of these systems enhanced drug solubility and tumor-targeting accumulation, thereby improving the therapeutic efficacy against colorectal cancer in vivo (tumor suppression rates of up to 81%). Interestingly, although the prepared material exhibited controlled release at pH 7.4, a burst release of the drug was observed during the first 12 h, which was attributed to the dissociation of the PGA-SN-38 prodrug from the nanopolyelectrolyte complexes due to the partial instability of the chitosan-based nanocomplexes in phosphate-buffered saline medium. The standardization and scale-up of polyelectrolyte complexes obtained through bottom-up methodologies is still a great challenge and necessitates carrying out arduous experimentation, since it depends on multiple intrinsic and extrinsic variables [181]. A means to improve the main problems generated during polyelectrolytic complexation, such as obtaining large particle sizes and highly polydisperse systems, is to employ top-down methods instead [182]. However, top-down methods such as high-or ultra-high-pressure homogenization also have some disadvantages, such as the chemical degradation of the material by excessive energy applied during the disaggregation process [183]. Nevertheless, the great benefit of these techniques is that the conditions implemented can be easily reproduced and scaled-up to industrial level. Thus, the polyelectrolyte complexes developed under these methodologies are suitable for easy technology transfer. Finally, the opportunities offered by nanotechnology in the health sector are also accompanied by challenges in the regulation of these products. One example of these concerns is the modification of the physicochemical properties of nanomaterials, which can lead to altered toxicity, solubility, and bioavailability profiles [184][185][186]. In addition, evidence regarding the potential safety issues of synthetic polymers appears to be the main driver of research on the use of natural polysaccharides in the application of more recent responsive polyelectrolyte complexes as drug delivery systems. Furthermore, the existence of strong regional differences in the regulation of nanomedicines confirms the need for the harmonization of information requirements for nanospecific properties. Current efforts are directed towards gaining sufficient knowledge on the quality, safety, and efficacy of nanomaterials to support regulatory decisions and enable a smooth transition to clinical applications (Table 7).

Block Copolymer Systems Based on Weak Polyelectrolytes

Block copolymers (BCPs) based on weak polyelectrolytes combine a stimuli-respon sive nature with native self-assembling properties, positioning them as an interesting clas of polymeric nano-units for use in structured materials. The incorporation of weak poly ion blocks in BCP facilitates copolymer synthesis and characterization while leading to weak bonding interactions (i) that facilitate the reversible conformation of the self-assem bled nanostructures with reversible complexation abilities (ii) towards drugs, metalli

Block Copolymer Systems Based on Weak Polyelectrolytes

Block copolymers (BCPs) based on weak polyelectrolytes combine a stimuli-respon sive nature with native self-assembling properties, positioning them as an interesting clas of polymeric nano-units for use in structured materials. The incorporation of weak poly ion blocks in BCP facilitates copolymer synthesis and characterization while leading to weak bonding interactions (i) that facilitate the reversible conformation of the self-assem bled nanostructures with reversible complexation abilities (ii) towards drugs, metallic

Block Copolymer Systems Based on Weak Polyelectrolytes

Block copolymers (BCPs) based on weak polyelectrolytes combine a stimuli-respon sive nature with native self-assembling properties, positioning them as an interesting clas of polymeric nano-units for use in structured materials. The incorporation of weak poly ion blocks in BCP facilitates copolymer synthesis and characterization while leading to weak bonding interactions (i) that facilitate the reversible conformation of the self-assem bled nanostructures with reversible complexation abilities (ii) towards drugs, metallic Vectors for protein, peptide, and gene delivery [198,199] Carboxymethyl starch ALG Drug delivery Mucoadhesive [191,192] Pectin Mucoadhesive, i.e., nasal inserts, ocular delivery Oral drug delivery [193] [194] [195] Carrageenan Drug delivery Selective protein adsorption [196] [197] HA Vectors for protein, peptide, and gene delivery [198,199] Carboxymethyl starch Oral drug delivery Protein carrier

Block Copolymer Systems Based on Weak Polyelectrolytes

Block copolymers (BCPs) based on weak polyelectrolytes combine a stimuli-respon sive nature with native self-assembling properties, positioning them as an interesting clas of polymeric nano-units for use in structured materials. The incorporation of weak poly ion blocks in BCP facilitates copolymer synthesis and characterization while leading to weak bonding interactions (i) that facilitate the reversible conformation of the self-assem bled nanostructures with reversible complexation abilities (ii) towards drugs, metalli

Block Copolymer Systems Based on Weak Polyelectrolytes

Block copolymers (BCPs) based on weak polyelectrolytes combine a stimuli-respon sive nature with native self-assembling properties, positioning them as an interesting class of polymeric nano-units for use in structured materials. The incorporation of weak poly ion blocks in BCP facilitates copolymer synthesis and characterization while leading to weak bonding interactions (i) that facilitate the reversible conformation of the self-assem bled nanostructures with reversible complexation abilities (ii) towards drugs, metallic

Block Copolymer Systems Based on Weak Polyelectrolytes

Block copolymers (BCPs) based on weak polyelectrolytes combine a stimuli-responsive nature with native self-assembling properties, positioning them as an interesting class of polymeric nano-units for use in structured materials. The incorporation of weak polyion blocks in BCP facilitates copolymer synthesis and characterization while leading to weak bonding interactions (i) that facilitate the reversible conformation of the self-assembled nanostructures with reversible complexation abilities (ii) towards drugs, metallic ions, and other types of cargo. Since the charge density on weak polyelectrolyte is pH-dependent, it also enables the dissolution of hydrophobic/ionic BCPs in polar organic solvents [204,205]. Emerging applications include agents that can be used to stabilize and/or deliver dugs, peptides, and small molecules; lubricants; colloids; the patterning of nanostructures; the synthesis of stimuli-responsive capsules; and filtration membranes [206][207][208][209]. Chemically, BCPs are formed by the covalent linkage of two or more distinct monomer units grouped in discrete blocks along the polymeric chain [210]. Due to the rapid progress made in polymer synthetic strategies and techniques such as controlled polymerization along with facile post-polymerization functionalization, BCPs with well-defined molecular weights and macromolecular architectures can be synthesized [211]. The exceptional compositional and molecular structural versatility of BCPs has facilitated the tremendous growth and application of new synthetic routes, enabling high levels of architectural complexity to be achieved. Examples of weak PE-based BCPs are given in Table 8. This section will highlight the scope of BCP-based PEs and their applications.

Directed Self-Assembly of BCPs for Nanopatterning

BCP self-assembly is a versatile process where the incompatible behavior of the constituent blocks leads to the formation of microstructures with a plethora of potential applications [211][212][213]. In this context, the directed self-assembly of BCPs has revolutionized the modern nanoelectronics industry. It has facilitated long-ordered structures, the transfer of patterns, and the design of low-resolution high-density nanostructures for high-level computing applications (Figure 7). There are some major drawbacks of current nanofabrication techniques, such as photolithography (limited in feature size), electronbeam lithography (low throughput), and EUV lithography (high development costs) [210]. Owing to these challenges, a large volume of research is dedicated to the development of cost-effective nanofabrication technology. In this case, the BCP soft lithography method offers a very simple, scalable, cost-effective platform for use in nanoscale fabrication, where feature sizes and geometries can be tuned via the chain length and volume fraction of BCPs. BCPs can self-assemble into varied different nanostructures through microphase separation, which is driven by the enthalpy of the demixing of the constituent components of the BCPs, while the process is constrained by the chemical connectivity of the blocks [214]. BCPs can be tuned to self-assemble into desired nanostructured morphologies such as spheres, cylinders, lamellae, and gyroids by adjusting the volume fraction (f ) of the constituent blocks [212,214]. In addition, chemical heterogeneity between the BCP blocks allows the selective complexation of guest species (e.g., metal ions), thereby creating patterned etch masks that can be transferred into functional materials and surfaces (Figure 7

Membranes from Weak Polyelectrolytes BCP for Filtration

Global population growth, climate change and human activities have degraded the quality of fresh water to critical levels through the discharge of chemicals into ground and surface waters. For filtration BCP based membrane technology has gained great significance utilizing nanoscale features as the pores of the membrane granting hydraulic permeability and separation selectivity. The self-assembled nanostructure of BCPs in the equilibrium state offer their (1) utility in the fabrication of high-performance separation membranes, (2) limitless chemical compositions, and (3) scalable nanomanufacturing processes. Manipulation of the nanostructure of BCP membranes provides wide range of limits of size-selection, ability to tailor the surface chemistry of BCP membranes for different water reuse. BCP membranes rely on the swelling of blocks of copolymer to narrow the pore size into the nanofiltration regime. In that context, the response of weak polyelectrolyte blocks (e.g., PAA, P2VP) to salt and pH stimuli via conformational changes that changes the pore geometry is critical (Scheme 1 and Figure 9

Membranes from Weak Polyelectrolytes BCP for Filtration

Global population growth, climate change and human activities have degraded the quality of fresh water to critical levels through the discharge of chemicals into ground and surface waters. For filtration BCP based membrane technology has gained great signifi cance utilizing nanoscale features as the pores of the membrane granting hydraulic per meability and separation selectivity. The self-assembled nanostructure of BCPs in the equilibrium state offer their (1) utility in the fabrication of high-performance separation membranes, (2) limitless chemical compositions, and (3) scalable nanomanufacturing pro cesses. Manipulation of the nanostructure of BCP membranes provides wide range of lim its of size-selection, ability to tailor the surface chemistry of BCP membranes for differen water reuse. BCP membranes rely on the swelling of blocks of copolymer to narrow the pore size into the nanofiltration regime. In that context, the response of weak polyelectro lyte blocks (e.g., PAA, P2VP) to salt and pH stimuli via conformational changes tha changes the pore geometry is critical (Scheme 1 and Figure 9) [207]. BCP membranes in the nanofiltration regime have also been obtained through the blending of different BCPs such A-B/A-C type blocks. For example, the combination o PS-P4VP and PS-PAA BCPs allows the formation of smaller pore sizes due to the hydro gen bonding occurring between PAA and P4VP blocks of the two blends. These complexes BCP membranes in the nanofiltration regime have also been obtained through the blending of different BCPs such A-B/A-C type blocks. For example, the combination of PS-P4VP and PS-PAA BCPs allows the formation of smaller pore sizes due to the hydrogen bonding occurring between PAA and P4VP blocks of the two blends. These complexes drive the morphological shift towards densely packed spherical nanostructures that form pores with smaller dimensions [233]. Another relatively easy process used to fabricate membranes is non-solvent-induced phase separation (NIPS) [234], where a concentrated BCP solution is casted on a polished surface and subsequently immersed in a non-solvent, leading to phase separation. In the case of BCPs, this process results in solvent evaporation and microphase separation, yielding unsymmetrical membrane where a mesoporous skin layer is supported by a macroporous bulk structure. NIPS has been employed in membrane preparation from PS-b-P4VP [235] and polystyrene-block-poly(N,N-dimethylaminoethyl methacrylate) (PS-b-PDMAEMA) BCPs [236]. In the latter example, the PDMAEMA block in the membrane is pH-and temperature-responsive, modulating the pore size in the range of 20-80 nm because of swelling/de-swelling. In PS-b-P4VP-based membranes, the P4VP segment is deprotonated at higher pH values and collapsed onto the pore walls, yielding a higher water permeability and larger pore sizes. Moreover, in PS-b-P4VP, the complexation of the P4VP segment with metal ions in the casting solution provides control over the pore formation [237].

Closing Remarks

From surface platforms to colloids and gels, weak polyelectrolytes have enabled the development of a wide range of functional materials owing to their intrinsic response to stimuli, including solvents, temperature, pH, and salt. This review paper is focused on the latter two stimuli, as these simple parameters are able to induce several responses in weak polyelectrolyte chains at the molecular level, including changes in their ionization rate, charge density, H-bonding ability, and conformation. In turn, these changes are transmitted to the supra-molecular level, enabling nano-systems to respond in precisely predetermined manners, including by changing their stability, their structure, and their physical properties (adhesion, solubility, physisorption, mechanical properties, etc.). Furthermore, the chemical variety of weak polyelectrolytes, encompassing natural and synthetic polymers with a range of available chemical functions and ionization constants (pKa and pKaH), enables the creation of varied biocompatible materials and vectors that hold promise for use in future biomedicine and pharmaceutical applications. Given this background and considering that weak polyelectrolytes can rely on external stimuli as both assembly and post-assembly triggers, it is not surprising that they stand as promising tools for the nanoarchitectonics of organic and hybrid systems. This dual role enables weak polyelectrolytes to act not only as functional nano-units conferring "smart" stimuli-responsiveness to materials but also as structure-directing agents. In this respect, prominent future developments can be anticipated in at least two research directions. First, pharmaceutically relevant systems based on biocompatible weak polyelectrolytes will gain improved precision and (multi)functionality for target drug delivery. Concurrently, nanostructured systems, including those based on BCP, will enable the confinement of weak polyacid/polybase nanodomains and pores to support the emergence of next-generation membranes and electrodes for use in energy and environment sustainability devices.

Closing Remarks

From surface platforms to colloids and gels, weak polyelectrolytes have enabled the development of a wide range of functional materials owing to their intrinsic response to stimuli, including solvents, temperature, pH, and salt. This review paper is focused on the latter two stimuli, as these simple parameters are able to induce several responses in weak polyelectrolyte chains at the molecular level, including changes in their ionization rate, charge density, H-bonding ability, and conformation. In turn, these changes are transmitted to the supra-molecular level, enabling nano-systems to respond in precisely predetermined manners, including by changing their stability, their structure, and their physical properties (adhesion, solubility, physisorption, mechanical properties, etc.). Furthermore, the chemical variety of weak polyelectrolytes, encompassing natural and synthetic polymers with a range of available chemical functions and ionization constants (pKa and pKaH), enables the creation of varied biocompatible materials and vectors that hold promise for use in future biomedicine and pharmaceutical applications. Given this background and considering that weak polyelectrolytes can rely on external stimuli as both assembly and post-assembly triggers, it is not surprising that they stand as promising tools for the nanoarchitectonics of organic and hybrid systems. This dual role enables weak polyelectrolytes to act not only as functional nano-units conferring "smart" stimuli-responsiveness to materials but also as structure-directing agents. In this respect, prominent future developments can be anticipated in at least two research directions. First, pharmaceutically relevant systems based on biocompatible weak polyelectrolytes will gain improved precision and (multi)functionality for target drug delivery. Concurrently, nanostructured systems, including those based on BCP, will enable the confinement of weak polyacid/polybase nanodomains and pores to support the emergence of next-generation membranes and electrodes for use in energy and environment sustainability devices.

Encapsulation Drug delivery

[247]

Closing Remarks

From surface platforms to colloids and gels, weak polyelectrolytes have enabled the development of a wide range of functional materials owing to their intrinsic response to stimuli, including solvents, temperature, pH, and salt. This review paper is focused on the latter two stimuli, as these simple parameters are able to induce several responses in weak polyelectrolyte chains at the molecular level, including changes in their ionization rate, charge density, H-bonding ability, and conformation. In turn, these changes are transmitted to the supra-molecular level, enabling nano-systems to respond in precisely predetermined manners, including by changing their stability, their structure, and their physical properties (adhesion, solubility, physisorption, mechanical properties, etc.). Furthermore, the chemical variety of weak polyelectrolytes, encompassing natural and synthetic polymers with a range of available chemical functions and ionization constants (pKa and pKaH), enables the creation of varied biocompatible materials and vectors that hold promise for use in future biomedicine and pharmaceutical applications. Given this background and considering that weak polyelectrolytes can rely on external stimuli as both assembly and post-assembly triggers, it is not surprising that they stand as promising tools for the nanoarchitectonics of organic and hybrid systems. This dual role enables weak polyelectrolytes to act not only as functional nano-units conferring "smart" stimuli-responsiveness to materials but also as structure-directing agents. In this respect, prominent future developments can be anticipated in at least two research directions. First, pharmaceutically relevant systems based on biocompatible weak polyelectrolytes will gain improved precision and (multi)functionality for target drug delivery. Concurrently, nanostructured systems, including those based on BCP, will enable the confinement of weak polyacid/polybase nanodomains and pores to support the emergence of next-generation membranes and electrodes for use in energy and environment sustainability devices.

Scheme 1 .

 1 Scheme 1. Responses of weak polyelectrolytes to stimuli. Schematic depiction of the molecular and conformational responses of weak polyelectrolytes bearing either weak acid (AH) or basic (B) moieties with respect to pH and salt stimuli.

Figure 1 .

 1 Figure 1. Weak polyelectrolyte as a multipotent tool for stimuli-responsive systems. Depiction of the different usages of weak polyelectrolytes for supporting the nanoarchitectonic design of functional materials addressing stimuli-responsive applications.

Figure 1 .

 1 Figure 1. Weak polyelectrolyte as a multipotent tool for stimuli-responsive systems. Depiction of the different usages of weak polyelectrolytes for supporting the nanoarchitectonic design of functional materials addressing stimuli-responsive applications.

Figure 2 .

 2 Figure 2. Film electrodeposition.Main approaches for electrochemically induced film formatio include electropolymerization, electrochemically induced precipitation, and coupling. The resultin surface coatings containing weak polyelectrolytes combine pH responsiveness (A) with eventua self-organization (B) and combination with inorganic units (C). Figures (A)[START_REF] Rydzek | Morphogen-Driven Self-Construction of Covalent Films Built from Polyelectrolytes and Homobifunctional Spacers: Buildup and PH Response[END_REF], (B)[START_REF] Song | Electrochemical Self-Assembly of Nano-Polyaniline Film by Forced Convection and Its Capacitive Performance[END_REF], and (C[START_REF] Sciortino | Electro-Click Construction of Hybrid Nanocapsule Films with Triggered Delivery Properties[END_REF] are reproduced with permission from the original articles.

Figure 2 .

 2 Figure 2. Film electrodeposition. Main approaches for electrochemically induced film formation include electropolymerization, electrochemically induced precipitation, and coupling. The resulting surface coatings containing weak polyelectrolytes combine pH responsiveness (A) with eventual self-organization (B) and combination with inorganic units (C). Figures (A)[START_REF] Rydzek | Morphogen-Driven Self-Construction of Covalent Films Built from Polyelectrolytes and Homobifunctional Spacers: Buildup and PH Response[END_REF], (B)[START_REF] Song | Electrochemical Self-Assembly of Nano-Polyaniline Film by Forced Convection and Its Capacitive Performance[END_REF], and (C)[START_REF] Sciortino | Electro-Click Construction of Hybrid Nanocapsule Films with Triggered Delivery Properties[END_REF] are reproduced with permission from the original articles.

Molecules 2022 ,Figure 3 .

 20223 Figure 3. LbL film buildup. (A) Depiction of the layer-by-layer film assembly str polyelectrolytes. Sequential adsorption of oppositely charged species on a substrate control of the film thickness and composition. (B) The resulting surface coatings polyelectrolytes can embed drugs either during or post-assembly through nontions. Figures (A)[START_REF] Decher | Fuzzy Nanoassemblies: Toward Layered Polymeric Multicomposites[END_REF] and (B)[START_REF] Bataglioli | Interplay of the Assembly Conditions on Drug Transport Mechanisms in Polyelectrolyte Multilayer Films[END_REF] are reproduced with permission from the origin

Figure 3 .

 3 Figure 3. LbL film buildup. (A) Depiction of the layer-by-layer film assembly strategy applied to polyelectrolytes. Sequential adsorption of oppositely charged species on a substrate enables the fine control of the film thickness and composition. (B) The resulting surface coatings containing weak polyelectrolytes can embed drugs either during or post-assembly through non-covalent interactions.Figures (A)[START_REF] Decher | Fuzzy Nanoassemblies: Toward Layered Polymeric Multicomposites[END_REF] and (B)[START_REF] Bataglioli | Interplay of the Assembly Conditions on Drug Transport Mechanisms in Polyelectrolyte Multilayer Films[END_REF] are reproduced with permission from the original articles.

  and energy storage[123] or as antioxidants[124]. Recent development has gradually evolved from prominently using synthetic strong polyelectrolytes to weaker ones, including using PAH, PEI, PLL, and poly(N-isopropylacrylamide) (PNIPAM) as polycations, as well as PMAA and PAA as polyanions. The corresponding colloidal systems benefited from the better control and fine tuning of intermolecular forces by pH modulation [125], ionic strength [126], and temperature [127]. Biosourced charged species such as dextran [128], CHI [129], bovine serum albumin [130], and DNA have also been used as functional units to encapsulate and deliver genetic cargo [128] and even co-deliver a drug [131]. Molecules 2022, 27, x FOR PEER REVIEW 11 of 32 oxidants [124]. Recent development has gradually evolved from prominently using synthetic strong polyelectrolytes to weaker ones, including using PAH, PEI, PLL, and poly(Nisopropylacrylamide) (PNIPAM) as polycations, as well as PMAA and PAA as polyanions. The corresponding colloidal systems benefited from the better control and fine tuning of intermolecular forces by pH modulation [125], ionic strength [126], and temperature [127]. Biosourced charged species such as dextran [128], CHI [129], bovine serum albumin [130], and DNA have also been used as functional units to encapsulate and deliver genetic cargo [128] and even co-deliver a drug [131].

Figure 4 .

 4 Figure 4. LbL elaboration of capsules. Schematic representation of the different synthesis pathways available to design weak polyelectrolyte multilayer capsules.

Figure 4 .

 4 Figure 4. LbL elaboration of capsules. Schematic representation of the different synthesis pathways available to design weak polyelectrolyte multilayer capsules.

  sules[132]. With the combination of magnetic nanoparticles such as iron oxide and electromagnetic fields of various frequency and power, different mechanisms can be induced. At low frequencies, a non-heating process can preferably be used to preserve tissues as well as a bioactive payload such as enzymes or DNA[138]. Meanwhile, with higher-frequency magnetic fields, a high increase in the local temperature induces the destruction of cellular and subcellular structures[139].

  ]. With the combination of magnetic nanoparticles such as iron oxide and electromagnetic fields of various frequency and power, different mechanisms can be induced. At low frequencies, a non-heating process can preferably be used to preserve tissues as well as a bioactive payload such as enzymes or DNA[138]. Meanwhile, with higher-frequency magnetic fields, a high increase in the local temperature induces the destruction of cellular and subcellular structures[139].

  ]. With the combination of magnetic nanoparticles such as iron oxide and electromagnetic fields of various frequency and power, different mechanisms can be induced. At low frequencies, a non-heating process can preferably be used to preserve tissues as well as a bioactive payload such as enzymes or DNA[138]. Meanwhile, with higher-frequency magnetic fields, a high increase in the local temperature induces the destruction of cellular and subcellular structures[139].

  ]. With the combination of magnetic nanoparticles such as iron oxide and electromagnetic fields of various frequency and power, different mechanisms can be induced. At low frequencies, a non-heating process can preferably be used to preserve tissues as well as a bioactive payload such as enzymes or DNA[138]. Meanwhile, with higher-frequency magnetic fields, a high increase in the local temperature induces the destruction of cellular and subcellular structures[139].

  ]. With the combination of magnetic nanoparticles such as iron oxide and electromagnetic fields of various frequency and power, different mechanisms can be induced. At low frequencies, a non-heating process can preferably be used to preserve tissues as well as a bioactive payload such as enzymes or DNA[138]. Meanwhile, with higher-frequency magnetic fields, a high increase in the local temperature induces the destruction of cellular and subcellular structures[139].

  ]. With the combination of magnetic nanoparticles such as iron oxide and electromagnetic fields of various frequency and power, different mechanisms can be induced. At low frequencies, a non-heating process can preferably be used to preserve tissues as well as a bioactive payload such as enzymes or DNA[138]. Meanwhile, with higher-frequency magnetic fields, a high increase in the local temperature induces the destruction of cellular and subcellular structures[139].

Figure 5 .

 5 Figure 5. Pharmaceutical requirements. Schematic representation of the different possible mechanisms induced by pH, ion concentration, and ion valency changes in a PAH/PMAA saloplastic. Reproduced with permission from the original article [8].

Figure 5 .

 5 Figure 5. Pharmaceutical requirements. Schematic representation of the different possible mechanisms induced by pH, ion concentration, and ion valency changes in a PAH/PMAA saloplastic.Reproduced with permission from the original article[START_REF] Sciortino | Saloplastics as Multiresponsive Ion Exchange Reservoirs and Catalyst Supports[END_REF].

Figure 6 .

 6 Figure 6. Pharmaceutical requirements. Examples of current challenges in drug delivery.

Figure 6 .

 6 Figure 6. Pharmaceutical requirements. Examples of current challenges in drug delivery.

Figure 6 .

 6 Figure 6. Pharmaceutical requirements. Examples of current challenges in drug delivery.

Scheme 2 .

 2 Scheme 2. Weak polyelectrolyte complexes for pharmaceutical vectorization. A schematic diagram of different stimuli and possible responses triggering drug delivery in weak polyelectrolyte complexes.

  ) [213,215]. Accordingly, systems based on BCP containing weak polyelectrolyte blocks such as poly(styrene-b-4vinylpyridene) (PS-b-P4VP) [216] and PS-b-P2VP [217] can be used to produce well-defined nanostructures with long-range ordered morphologies. Upon the infiltration of weak polyion block domains with metal ions (Ni + , Al 3+ and Cr 2+ ), well-defined metal-oxide nanodots and/or nanowires were obtained and used to define etch masks for pattern transfer [217]. For instance, we recently reported the use of a high-contrast etch mask for pattern transfer into silicon substrates through ICP/RIE etching for the development of vertically coupled plasmonic arrays for use in surface enhanced Raman scattering [213], anti-reflective surfaces, and photocatalysts [218]. Molecules 2022, 27, x FOR PEER REVIEW of a high-contrast etch mask for pattern transfer into silicon substrates through etching for the development of vertically coupled plasmonic arrays for use in sur hanced Raman scattering [213], anti-reflective surfaces, and photocatalysts [218].

Figure 7 .

 7 Figure 7. Self-assembled films and membranes from BCP. Schematic representation of terned systems enabled by BCP phase separation [219].

Figure 7 .

 7 Figure 7. Self-assembled films and membranes from BCP. Schematic representation of nanopatterned systems enabled by BCP phase separation [219].

5. 2 .

 2 Colloidal Systems from Weak Polyelectrolytes BCP for Drug Delivery BCP-containing polyelectrolytes blocks have been used to design nanometer-to micrometer-sized vectors for use in drug delivery and encapsulation applications through manipulating the physicochemical properties of shells to adjust their permeability (Figure 8) [220]. Stimuli such as pH and ionic strength changes create the opportunity to easily control drug-loaded vectors in open and closed states [221-223].

Figure 7 .

 7 Figure 7. Self-assembled films and membranes from BCP. Schematic terned systems enabled by BCP phase separation [219].

5. 2 .

 2 Colloidal Systems from Weak Polyelectrolytes BCP for Drug Deliv BCP-containing polyelectrolytes blocks have been used to d crometer-sized vectors for use in drug delivery and encapsulati manipulating the physicochemical properties of shells to adjust th 8) [220]. Stimuli such as pH and ionic strength changes create t control drug-loaded vectors in open and closed states [221-223].

Figure 8 .

 8 Figure 8. Self-assembled colloids from BCP. Schematic representation enabled by BCP for use in drug delivery applications. Reproduced with nal article [224].

Figure 8 .

 8 Figure 8. Self-assembled colloids from BCP. Schematic representation of nanostructured colloids enabled by BCP for use in drug delivery applications. Reproduced with permission from the original article [224]. Weak polyelectrolyte-based BCPs with constituent blocks such as carboxylic acids, phosphoric acid, and amines show different ionization degrees depending on the pH of the surrounding environment [225]. This leads to conformational changes due to different interactions among polymeric chains and also to the formation of hydrogen bonds. The solubility conditions and relative size of the blocks determine whether they will self-assemble into micelles, vesicles, or a combination of both [226]. When BCPs combine weak polyion blocks with hydrophobic blocks (e.g., PS-b-P2VP and poly(styrene-b-acrylic acid) (PS-b-PAA)), they form core-shell micelles in water with a hydrophobic core and polyelectrolyte corona [224]. Such systems enable solubilization of hydrophobic molecules into their core, while the external corona serves stabilizes vectors in aqueous media and helps binding to oppositely charged molecules, e.g., proteins. Conversely, changing the surface charge density of such micelles by pH-induced protonation of weak polyelectrolyte blocks controlled the stability or aggregation behavior of PS-b-P2VP-b-PEO [227], PB-b-P2VP-b-PMAA [228], and PCL-b-PEO-b-P2VP)[229] micelles. In contrast, when BCPs combine weak polyion blocks with hydrophobic blocks, they can micellize on complexation of their polyelectrolyte block with oppositely charged partners (polycations, drugs, multivalent cations) as reported for poly(ethylene glycol-b-2-(dimethylamino)ethylmethacrylate) (PEG-b-PDMAEMA), poly(acrylic acid-b-ethylene oxide) (PAA-b-PEO) and PNIPAM-b-PAA [230,231]. Such micelles recently found applications as drug vectors and sol-gel structure directing agents with pH-triggered stability [232].

  ) [207]. cations) as reported for poly(ethylene glycol-b-2-(dimethylamino)ethylmethacrylate (PEG-b-PDMAEMA), poly(acrylic acid-b-ethylene oxide) (PAA-b-PEO) and PNIPAM-b PAA [230,231]. Such micelles recently found applications as drug vectors and sol-ge structure directing agents with pH-triggered stability [232].

Figure 9 .

 9 Figure 9. Self-assembled membranes from BCP. Schematic representation of nanostructured mem branes enabled by BCP and their stimuli-responsive adaptation for filtration membranes. Mi crophase separation of PS-b-P4VP yields pores of P4VP that can be post-functionalized by 1,4-diio dobutane (DIB) and methyl iodide (MeI), controlling their hydrophilicity. Reproduced with permis sion from the original article [207].

Figure 9 .

 9 Figure 9. Self-assembled membranes from BCP. Schematic representation of nanostructured membranes enabled by BCP and their stimuli-responsive adaptation for filtration membranes. Microphase separation of PS-b-P4VP yields pores of P4VP that can be post-functionalized by 1,4-diiodobutane (DIB) and methyl iodide (MeI), controlling their hydrophilicity. Reproduced with permission from the original article [207].

Table 2 .

 2 Typical brushes based on weak polyelectrolytes and emerging applications.

	Target Application	Brushes Type	Reference
	Reversible adhesion	PDMAEMA/PMAA poly(N,N-dimethylacrylamide)/PAA	[51] [50]
	Bio-adhesion	PMAA PAA	[54] [55]
		PAA/PMAA	[48]
	Cargo immobilization/release	PDMAEMA	[56]
		PDMAEMA/PAA	[52]
	Antifouling	P4VP PMAA	[57] [58]

Table 3 .

 3 Typical electrodeposited systems based on weak polyelectrolytes and emerging applications.

	Electrodeposition Principle	Polyelectrolyte Type and Typical Conditions (vs. Ag/AgCl)	Applications	Reference
	Electrocoupling by click
	reaction

P AA or PAH grafted with alkyne and azide (-0.3 V, 0.6 mM CuSO 4 , H 2 O)

Table 4 .

 4 Typical LbL multilayer films based on weak polyelectrolytes and emerging applications.

	Target Application	Multilayer Type	References
		PAH/PAA	[95]
	Cargo encapsulation and release	PEI/PAA	[114]
		CHI/HA and PLL/PGA	[115]
	Tunable bio-interface	PAH/PAA CHI/PGA	[116] [117]
	Slippery liquid-infused porous surface	(PEI/PAH)/PAA PEI/Nafion	[106] [107]
	Methylmercaptan gas sensor	(PAH-Ag + )/PAA	[113]
	Urea sensor	PEI/Urease/reduced graphene oxide	[118]
	Ionic conductivity	PDADMA/PAA	[110]
	Ionic current rectification	PLL/PAA and PEI/PAA	[109]
	Ion selective ultrafiltration	CHI/Chondroitin sulfate	[111]
	Micropollutant filtration	PAH/PAA	[119]
	Solvent resistant nanofiltration membrane	PAH/PAA	[120]

Table 5 .

 5 Recently reported weak polyelectrolytes used in the development of multilayered nanocarriers and emerging applications.

	Molecules 2022, 27, x FOR PEER REVIEW			12 of 33
	Applications	Weak Polyelectrolyte Used	Chemical Structures	References
	PAH

Table 5 .

 5 Recently reported weak polyelectrolytes used in the development of multilayered nanocarriers and emerging applications.

	Applications	Weak Polyelectrolyte Used	Chemical Structures	References
	PAH		Drug delivery, imaging	[140,141]
	PEI		Drug delivery, gene delivery	[142,143]
	PMAA and PAA		Drug delivery, cancer therapy	[128,134,144]
	Poly(N-isopropylacrylamide) (PNIPAM)		Drug delivery	[144]
	Poly-L-Arginine		Hyperthermia, imaging, drug deliv-ery, gene silencing/editing	[145-150]
	PLL		Hyperthermia, imaging	[139,151]

Table 5 .

 5 Recently reported weak polyelectrolytes used in the development of multilayered nanocarriers and emerging applications.

	Applications	Weak Polyelectrolyte Used	Chemical Structures	References
	PAH		Drug delivery, imaging	[140,141]
	PEI		Drug delivery, gene delivery	[142,143]
	PMAA and PAA		Drug delivery, cancer therapy	[128,134,144]
	Poly(N-isopropylacrylamide) (PNIPAM)		Drug delivery	[144]
	Poly-L-Arginine		Hyperthermia, imaging, drug deliv-ery, gene silencing/editing	[145-150]
	PLL		Hyperthermia, imaging	[139,151]

Table 5 .

 5 Recently reported weak polyelectrolytes used in the development of multilayered nanocarriers and emerging applications.

	Applications	Weak Polyelectrolyte Used	Chemical Structures	References
	PAH		Drug delivery, imaging	[140,141]
	PEI		Drug delivery, gene delivery	[142,143]
	PMAA and PAA		Drug delivery, cancer therapy	[128,134,144]
	Poly(N-isopropylacrylamide) (PNIPAM)		Drug delivery	[144]
	Poly-L-Arginine		Hyperthermia, imaging, drug deliv-ery, gene silencing/editing	[145-150]
	PLL		Hyperthermia, imaging	[139,151]

Table 5 .

 5 Recently reported weak polyelectrolytes used in the development of multilayered nanocarriers and emerging applications.

	Applications	Weak Polyelectrolyte Used	Chemical Structures	References
	PAH		Drug delivery, imaging	[140,141]
	PEI		Drug delivery, gene delivery	[142,143]
	PMAA and PAA		Drug delivery, cancer therapy	[128,134,144]
	Poly(N-isopropylacrylamide) (PNIPAM)		Drug delivery	[144]
	Poly-L-Arginine		Hyperthermia, imaging, drug deliv-ery, gene silencing/editing	[145-150]
	PLL		Hyperthermia, imaging	[139,151]

Table 5 .

 5 Recently reported weak polyelectrolytes used in the development of multilayered nanocarriers and emerging applications.

	Applications	Weak Polyelectrolyte Used	Chemical Structures	References
	PAH		Drug delivery, imaging	[140,141]
	PEI		Drug delivery, gene delivery	[142,143]
	PMAA and PAA		Drug delivery, cancer therapy	[128,134,144]
	Poly(N-isopropylacrylamide) (PNIPAM)		Drug delivery	[144]
	Poly-L-Arginine		Hyperthermia, imaging, drug deliv-ery, gene silencing/editing	[145-150]
	PLL		Hyperthermia, imaging	[139,151]

Table 5 .

 5 Recently reported weak polyelectrolytes used in the development of multilayered nanocarriers and emerging applications.

	Applications	Weak Polyelectrolyte Used	Chemical Structures	References
	PAH		Drug delivery, imaging	[140,141]
	PEI		Drug delivery, gene delivery	[142,143]
	PMAA and PAA		Drug delivery, cancer therapy	[128,134,144]
	Poly(N-isopropylacrylamide) (PNIPAM)		Drug delivery	[144]
	Poly-L-Arginine		Hyperthermia, imaging, drug deliv-ery, gene silencing/editing	[145-150]
	PLL		Hyperthermia, imaging	[139,151]

Table 6 .

 6 Recently

	Polyelectrolyte Complex	Processing	Target Applications	References
	PAH/PAA	Compacted by ultracentrifu-	Catalysis support	[164]

reported weak polyelectrolytes complexes processed into gels and emerging applications.

Table 6 .

 6 Recently reported weak polyelectrolytes complexes processed into gels and emerging applications.

	Polyelectrolyte Complex	Processing	Target Applications	References
	PAH/PAA	Compacted by ultracentrifugation	Catalysis support	[164]
	CHI/ALG			[162]
	β-Cyclodextrin-CHI/ALG	Compacted by ultracentrifugation	Biomaterials and biomedical applications	[163]
	poly[triethyl(4-vinylbenzyl)ammonium/ALG			[161]
	PAH/PMAA	Compacted by centrifugation	Sorption of transition metal ions Catalysis support	[158] [8]
	Poly(vinyl alcohol)/PAA	Injection	Adhesives	[159]
	CHI/ALG	Sedimentation	Tissue engineering	[160]
	PEI/PSS			[165]
	PDADMAC/PAA	Aqueous phase Separation	Filtration membranes	[13]
	PAH/PSS			[166]

Table 7 .

 7 Recently reported weak polyelectrolytes used in the design of polyelectrolyte complexes for drug delivery and biomedical research applications.

	Molecules 2022, 27, x FOR PEER REVIEW			17 of 33
	Polyelectrolyte	Chemical Structure	Applications	References
	CHI

Table 7 .

 7 Recently reported weak polyelectrolytes used in the design of polyelectrolyte complexes for drug delivery and biomedical research applications.

	Molecules 2022, 27, x FOR PEER REVIEW				17 of 33
	Polyelectrolyte	Chemical Structure	Applications	References
			Oral drug delivery Mucosal delivery Oral drug delivery Mucosal delivery	[179] [187]	[179] [187]
	CHI	R = H or COCH3 R = H or COCH 3	Gene delivery Cancer therapy Anti HVI therapy Gene delivery Cancer therapy Anti HVI therapy	[188] [189] [190]	[188] [189] [190]
	ALG
	ALG		Drug delivery Mucoadhesive	[191,192]

Pectin

Mucoadhesive, i.e., nasal inserts, ocular delivery Oral drug delivery

[193] [194] [195]

Table 7 .

 7 Recently reported weak polyelectrolytes used in the design of polyelectrolyte complexes for drug delivery and biomedical research applications.

	Polyelectrolyte	Chemical Structure	Applications	References
			Oral drug delivery	[179]
			Mucosal delivery	[187]
	CHI		Gene delivery	[188]
			Cancer therapy	[189]
		R = H or COCH3	Anti HVI therapy	[190]
	ALG		Drug delivery Mucoadhesive Drug delivery Mucoadhesive	[191,192] [191,192]

Pectin

Mucoadhesive, i.e., nasal inserts, ocular delivery Oral drug delivery

[193] [194] [195]

Drug delivery

[196]

Table 7 .

 7 Cont.

	CHI		Gene delivery Cancer therapy	[188] [189]
		R = H or COCH3	Anti HVI therapy	[190]
	ALG		Drug delivery Mucoadhesive	[191,192]
	Polyelectrolyte	Chemical Structure	Applications	References
			Mucoadhesive, i.e., nasal in-	[193]
	Pectin Pectin		serts, ocular delivery	[194]
			Oral drug delivery	[195]
	Carrageenan		Drug delivery Selective protein adsorption	[196] [197]
	HA		Vectors for protein, peptide, and gene delivery	[198,199]
	Carboxymethyl starch		Oral drug delivery Protein carrier	[200] [201]
	PGA		Drug delivery Biomolecule carrier	[202] [203]

Data Availability Statement: Not applicable.

Acknowledgments: G.R thanks Hauru for fruitful discussions.

Table 8. Recently reported BCPs containing a weak polyelectrolyte block and their applications.

BCP Polyelectrolyte Chemical Structure Applications References

Poly(styrene)-b-poly(4-vinylpyridine) PS-b-P4VP

noethyl methacrylate) (PS-b-PDMAEMA) BCPs [236]. In the latter example, the PDMAEMA block in the membrane is pH-and temperature-responsive, modulating the pore size in the range of 20-80 nm because of swelling/de-swelling. In PS-b-P4VP-based membranes, the P4VP segment is deprotonated at higher pH values and collapsed onto the pore walls, yielding a higher water permeability and larger pore sizes. Moreover, in PS-b-P4VP, the complexation of the P4VP segment with metal ions in the casting solution provides control over the pore formation [237]. PDMAEMA block in the membrane is pH-and temperature-responsive, modulating the pore size in the range of 20-80 nm because of swelling/de-swelling. In PS-b-P4VP-based membranes, the P4VP segment is deprotonated at higher pH values and collapsed onto the pore walls, yielding a higher water permeability and larger pore sizes. Moreover, in PS-b-P4VP, the complexation of the P4VP segment with metal ions in the casting solution provides control over the pore formation [237].

BCP Polyelectrolyte Chemical Structure Applications

Author Contributions: This review was elaborated from contributions of all co-authors. All authors have read and agreed to the published version of the manuscript.

Funding: This research received no external funding.

Institutional Review Board Statement: Not applicable.

Informed Consent Statement: Not applicable.

Conflicts of Interest:

The authors declare no conflict of interest.

Abbreviations