Melih Argyro Zenetos

Ertan Çinar

Fabio Crocetta

Dani Golani

Antonietta Rosso

Gianna Servello

Noa Shenkar

Xavier Turon

Marc Verlaque

Melih Ertan Çinar

Uncertainties and validation of alien species catalogues: The Mediterranean as an example

Keywords:

à la diffusion de documents scientifiques de niveau recherche, publiés ou non, émanant des établissements d'enseignement et de recherche français ou étrangers, des laboratoires publics ou privés.

Introduction

Preparation of alien species (AS) checklists at larger scale and the development of relevant Web databases have been challenging tasks over the latest decades, with many funds and efforts being channeled in this direction from within the European Union (AquaNIS, DAISIE, EASIN, ESENIAS, NOBANIS). One major drawback in most of the aforementioned systems is the lack of long-term funding, which often results in a lack of updates which is, in turn, a prerequisite for delivering timely reliable information to environmental managers [START_REF] Lucy | INVASIVESNET towards an International Association for open knowledge on invasive alien Species[END_REF]. Nevertheless, the most serious problem is often the lack of expertise that ought to be engaged in such an endeavour. Species records in the existing data systems are often taken at face value without investigating their validity.

We acknowledge that no list at the national/regional scale is fully reliable unless it is the result of close collaboration of local experts. For instance, a list of AS in Greek waters that was first compiled in 2005 [START_REF] Pancucci-Papadopoulou | Update of marine alien species in Hellenic waters[END_REF], and then was updated in 2009 [START_REF] Zenetos | Aquatic alien species in Greece (2009): tracking sources, patterns and effects on the ecosystem[END_REF] and again in 2010 (Zenetos et al., 2011b), is today out of date and includes some inevitable errors. For molluscs alone, out of about 100 potential alien taxa that were examined, almost half of the records turned out to be based on misidentifications or incorrect distributional data (FC, AZ unpublished data). This phenomenon is accentuated when no experts are involved, or where checklists are only based on knowledge that is provided by scientists with broad taxonomic expertise.

Apart from the aforementioned species-specific knowledge, any checklist also requires continuous updating in the light of new information on: a) new arrivals or overlooked species, e.g. Bugula neritina (Linnaeus, 1758); b) range expansion of already known species resulting in change of their establishment success, e.g. the brown shrimp Penaeus aztecus Ives, 1891, which within six years invaded almost the entire Mediterranean since the first record from Turkey [START_REF] Deval | An unexpected find of the Western Atlantic shrimp, Farfantepenaeus aztecus (Ives, 1891) (Decapoda, Penaeidae) in Antalya Bay, Eastern Mediterranean Sea[END_REF], and whose status was switched from casual to invasive; c)changes in identification/nomenclature resulting from both traditional and molecular studies, e.g. the wellestablished and exploited prawn previously identified as Penaeus japonicus Spence Bate, 1888, and known in the Eastern Mediterranean since 1924 (Egypt: [START_REF] Balss | Zoological results of the Cambridge Expedition to the Suez Canal, 1924. XIV. Bericht über die Crustacea Decapoda (Natantia und Anomura)[END_REF], turned out to be Penaeus pulchricaudatus Stebbing, 1914[START_REF] Tsoi | Verification of the cryptic species Penaeus pulchricaudatus in the commercially important kuruma shrimp P. japonicus (Decapoda: Penaeidae) using molecular taxonomy[END_REF]. Moreover, some alien fish species that are well-established in the Mediterranean and widely reported in the past literature with specific binomial names turned out to be species that are novel to science: Etrumeus teres (DeKay, 1842) vs Etrumeus golanii [START_REF] Dibattista | Review of the round herrings of the genus Etrumeus (Clupeidae: Dussumieriinae) of Africa, with descriptions of two new species[END_REF]Saurida undosquamis (Richardson, 1848) vs Saurida lessepsianus [START_REF] Russell | Saurida lessepsianus a new species of lizardfish (Pisces: Synodontidae) from the Red Sea and Mediterranean Sea, with a key to Saurida species in the Red Sea[END_REF]Sillago sihama (Forsskål, 1775) vs Sillago suezensis [START_REF] Golani | Sillago suezensis, a new whiting from the northern Red Sea, and the status of Sillago erythraea Cuvier (Teleostei: Sillaginidae)[END_REF](see DiBattista et al., 2012;[START_REF] Russell | Saurida lessepsianus a new species of lizardfish (Pisces: Synodontidae) from the Red Sea and Mediterranean Sea, with a key to Saurida species in the Red Sea[END_REF][START_REF] Golani | CIESM e Atlas of Exotic Fishes e List[END_REF]; d) changes in alien or native status for cryptogenic species resulting from genetic and biogeographical studies, e.g. the alga Caulacanthus okamurae Yamada (see [START_REF] Verlaque | Macrophytes[END_REF], the polychaete Chaetozone corona Berkeley and Berkeley, 1941 (see Le [START_REF] Garrec | Chaetozone corona (Polychaeta, Cirratulidae) in the Bay of Biscay: a new alien species for rhe north-east Atlantic waters[END_REF]; e) changes in the introduction pathway resulting in the alteration of native/alien/cryptogenic status, e.g. the case of Aplysia dactylomela Rang, 1828, initially assumed to be ship transferred or unaided via Gibraltar [START_REF] Scuderi | Prima segnalazione di Aplysia dactylomela Rang, 1828 e probabile presenza di Syphonota geographica (Adams and Reeve, 1850) (Gastropoda: Opisthobranchia: Anaspidea) per le acque del Mediterraneo[END_REF], subsequently moved to the Lessepsian immigrant category (entering via the Suez Canal: [START_REF] Crocetta | The invasive spotted sea hare Aplysia dactylomela (Mollusca: Gastropoda: Aplysiidae)dnew records and spread pattern in the Mediterranean[END_REF], and was lately transferred to unknown category [START_REF] Valdes | The origin and dispersal pathway of the spotted sea hare Aplysia dactylomela (Mollusca: Opisthobranchia) in the Mediterranean Sea[END_REF].

Despite the aforementioned problems, several AS lists have been published in the past from the Mediterranean Sea as a whole, among which [START_REF] Galil | Alien species in the Mediterranean Seadwhich, when, where, why?[END_REF][START_REF] Galil | Taking stock: inventory of alien species in the Mediterranean Sea[END_REF], [START_REF] Nunes | Gateways to alien invasions in the European seas[END_REF], and [START_REF] Zenetos | Annotated list of alien marine species in the Mediterranean with emphasis on worst invasive species[END_REF][START_REF] Zenetos | Additions to the annotated list of marine alien biota in the Mediterranean with special emphasis on Foraminifera and Parasites[END_REF][START_REF] Huber | Compendium of Bivalves. A Full-color Guide to 3,300 of the World's Marine Bivalves[END_REF][START_REF] Davie | A review of Macrophthalmus sensu lato (Crustacea: Decapoda: Macrophtalmidae) from Australia, including two new species and new records[END_REF] are noteworthy. [START_REF] Galil | East is east and West is west? Management of marine bioinvasions in the Mediterranean Sea[END_REF] recently published an expansive article based on a critical review of historical data, highlighting how the global trend of increasing numbers of AS is magnified in the Mediterranean. Based on these data, realistic and practicable priority actions are proposed in order to reduce potential impediments that hinder the implementation of a mitigation programme of the biological invasions. This same work emphasises the need for accurate lists of AS and the urgency of reducing current geographical and taxonomical data gaps. However, the dataset that accompanies that work suffers itself from all of these problems, which can only be resolved with the assistance of active participation of taxonomic experts. The aim of the present work is to present an updated list of established AS in the Mediterranean, and to stress the need for collaboration with locals and the contribution of taxonomic experts to compile accurate lists of AS, which are a prerequisite for any mitigation action.

Methodology

As is well known to taxonomists and conservation biologists, taxonomic knowledge has widely increased in the last two to three decades due to the even wider use of combined approaches, and some species reported in the past literature (usually from 1700 to around 1980) have never actually lived in the area where they have been widely recorded in the past. Using as a starting point the most recent list of AS (that of [START_REF] Galil | East is east and West is west? Management of marine bioinvasions in the Mediterranean Sea[END_REF], we performed a critical update based on reviews by Mediterranean taxonomists on macroalgae, Mollusca, Polychaeta, Ascidiacea, Bryozoa and Fishes. An extensive literature survey constitutes the base of the present article. Published records of AS were sought in both indexed and non-indexed journals, as well as in the gray literature, since most of the historical journals are non-indexed and, particularly, faunal records are still being published in non-indexed journals, thus allowing only manual searching. Nomenclature of species, first record and establishment success were all checked by local experts.

The updates of the present work are as follows:

List of species . Some species that are listed are not alien and others have been excluded as they fall within one of the following criteria: i) species with unresolved taxonomic status (species complex or morphologically similar taxa) which may include native species; ii) records lacking proper scientific documentation; iii) cryptogenic species sensu [START_REF] Carlton | Biological invasions and cryptogenic species[END_REF]; iv) non-living records (i.e. loose valves, empty shells) unless known from the invaded area from a wide number of records/material; v) non-established spe-cies (AS, whose populations are known to be extinct or possibly not yet established, if the record is recent, i.e. within the past decade).

Taxonomic/nomenclatural issues. All species names were checked following the World Register of Marine Species (WoRMS Editorial Board, 2016). Discrepancies were reported to the WoRMS editors.

Establishment success . The terminology used varies among in-vasion biologists. For species reported once or twice on the basis of single individuals, we retain the term 'casual'. If there are many records, spread over time and place, it is assumed that the species are established, even though there may be no evidence of reproduction.

Year and country of the first record. For unpublished data, we searched museum collections and contacted the authors of the first report when possible. Dates of personal observations were also considered. We refer to the first recipient country according to the first collection/observation date, not according to reporting date.

Trends in introduction of new AS. We used the compiled data to ascertain the rate of new introductions, presented as a yearly rate (average per decade) since 1950. For the last six years (2011e2016) of the analysis, the number of recorded species is an underestimate. This is due to the time lag between the sampling of the species and the publication of the data, which can take several years. To better demonstrate the introduction rate, we also used the collection dates of non-established species (casual records) which have been reported after 1950.

Results and discussion

List of species

Our updated list of established aliens in the Mediterranean, includes species that i) are not included in the lists published by [START_REF] Galil | Alien species in the Mediterranean Seadwhich, when, where, why?[END_REF][START_REF] Galil | Taking stock: inventory of alien species in the Mediterranean Sea[END_REF], Zenetos et al. (2010[START_REF] Zenetos | Alien species in the Mediterranean Sea by 2012. A contribution to the application of European Union's Marine strategy framework directive (MSFD). Part 2. Introduction trends and pathways[END_REF], [START_REF] Nunes | Gateways to alien invasions in the European seas[END_REF], [START_REF] Galil | East is east and West is west? Management of marine bioinvasions in the Mediterranean Sea[END_REF]; ii) were reported after these inventories, and iii) species first recorded a long time ago, which were formerly considered native and whose alien status has been recently proven in the body of literature.

Seventy four of the reported species should be excluded, 37 as not true aliens, 36 as not established. Another 72 established species are added to the list of [START_REF] Galil | East is east and West is west? Management of marine bioinvasions in the Mediterranean Sea[END_REF]. These are: macroalgae (16 species), Mollusca (3), Polychaeta (28), Crustacea (4), Bryozoa (4), Ascidiacea (9), Cnidaria (3), Turbellaria (1); Sipuncula (1), and fishes (3). The total number of established AS in the Mediterranean is 613.

3.1.1. Species that should be excluded (misidentifications, unconfirmed, cryptogenic or non-established species)

According to the exclusion criteria of [START_REF] Galil | East is east and West is west? Management of marine bioinvasions in the Mediterranean Sea[END_REF], 37 species (6 macroalgae, 19 Mollusca, 7 Polychaeta, 1 Crustacea, 1 Bryozoa, 1 Cnidaria) which were reported as established should be removed from the Mediterranean aliens list of established species, either because their identification has been revised or because they are cryptogenic or questionable or based on empty shells (molluscan species). A further 36 species (9 macroalgae, 14 Mollusca, 2 Polychaeta,3 Crustacea,1 Ascidiacea,1 Echinodermata,7 Fish), should be removed from the list as the reports on them are based on casual records. We provide here a detailed explanation for each taxonomic/ecofunctional group. For each group we provide in brackets the number of the species that should be removed as not true aliens and the number of species whose reports are based on casual reports. 3.1.1.1. Macroalgae (6 þ 9). Five species namely Antithamnionella boergesenii (Cormaci & G.Furnari) Athanasiadis, Antithamnionella elegans (Berthold) J.H.Price & D.M.John, Antithamnionella spirographidis (Schiffner) E.M.Wollaston, A. ternifolia (J.D.Hooker & Harvey) Lyle and Caulerpa chemnitzia (Esper) J.V.Lamouroux are classified as debatable (cryptogenic) in [START_REF] Verlaque | Macrophytes[END_REF] and as such should be provisionally removed from the aliens' list of the Mediterranean Sea. Chondria collinsiana M.A.Howe, is a probable misidentification of Chondria curvilineata F.S. Collins & Hervey.

Acrochaetium robustum Børgesen, A. spathoglossi Børgesen, A. subseriatum Børgesen, Ascophyllum nodosum (Linnaeus) Le Jolis, Derbesia boergesenii (Iyengar & Ramanathan) Mayhoub, Fucus spiralis Linnaeus, Grateloupia patens (Okamura) S.Kawaguchi & H.W.Wang, Hypnea flagelliformis Greville ex J.Agardh, and Rhodymenia erythraea Zanardini, which are only known from the original records (see [START_REF] Verlaque | Macrophytes[END_REF], must also be excluded from the list. 3.1.1.2. Mollusca (19 þ 14). Records of Alectryonella crenulifera (Sowerby G.B. II, 1871) and Alectryonella plicatula (Gmelin, 1791) by Mienis et al. (2012a, b) were contemporarily confuted by Crocetta et al. (2015a) and [START_REF] Huber | Compendium of Bivalves 2. A Full-color Guide to the Remaining Seven Families[END_REF]. Based on molecular analysis of Mediterranean specimens, Crocetta et al. (2015a) suggested that both species belong to Dendostrea cf. folium (Linnaeus, 1758) while, based on shell morphology, [START_REF] Huber | Compendium of Bivalves 2. A Full-color Guide to the Remaining Seven Families[END_REF] suggested that they belong to Dendostrea rosacea (Deshayes, 1836). Mediterranean specimens originally ascribed to Murchisonella columna (Hedley, 1907) were recently described as Murchisonella mediterranea Pen ~as and Rolan, 2013, a Mediterranean endemic species (see Pen ~as and Rolan, 2013). The record of Cantharus tranquebaricus (Gmelin, 1791) was based on three empty shells from the Mediterranean coasts of Israel [START_REF] Mienis | Cantharus tranquebaricus first records of another Indian Ocean species in the Eastern Mediterranean[END_REF]. However, this record was subsequently amended by the same author [START_REF] Mienis | Marine molluscs from the Eastern Mediterranean 26. The first finds of Thais sacellum (Gmelin, 1791) in Israel[END_REF], who stated that the record was presumably based on three mislabelled specimens from India, and that the species should have been excluded from AS lists. The presence of Spondylus groschi Lamprell and Kilburn, 1995 and Spondylus multisetosus Reeve, 1856 is disputed due to the confusion with their congeneric S. spinosus Schreibers, 1793 [START_REF] Zenetos | CIESM Atlas of Exotic Species in the Mediterranean[END_REF]. Therefore, they are labelled as "questionable" species until their systematics is revised. The presence of Centrocardita akabana (Sturany, 1899) in the Mediterranean is dubious. Juvenile specimens of the eastern Atlantic-Mediterranean taxon Cardites antiquatus (Linnaeus, 1758) may be easily confused this alien taxon. Therefore, a DNA barcoding approach is required in order to support the presence of C. akabana in the Mediterranean. Dendrodoris fumata (Rüppell and Leuckart, 1830) was recorded from Israel [START_REF] Barash | Further additions to the knowledge of Indo-Pacific Mollusca in the Mediterranean Sea[END_REF], Turkey (Çevik et al., 2012) and Italy [START_REF] Trainito | Nudibranchi del Mediterraneo. 2 a edizione, riveduta ed ampliata[END_REF]. However, published identifications were never confirmed by molecular and/or anatomical data, and therefore remain all highly doubtful. The past presence of Zygochlamys patagonica (King, 1832) in the Mediterranean is now considered as very doubtful, as [START_REF] Mannino | An updated overview of the marine alien and cryptogenic species from the Egadi Islands Marine Protected Area (Italy)[END_REF] have excluded the records from the Alboran Sea and Italy since it is not sure if all Mediterranean records were based on discards in the field of dead specimens imported with shrimps (some presumably even with soft parts), or if specimens obtained 'from fishermen' may refer to trawlings from the Atlantic by fleets based only in the Mediterranean Sea.

In addition, although molluscan records based only on empty shells were included in [START_REF] Zenetos | CIESM Atlas of Exotic Species in the Mediterranean[END_REF] and subsequent lists, in accordance with [START_REF] Marchini | Recommendations on standardizing lists of marine alien species: lessons from the Mediterranean Sea[END_REF] they should be removed from all Mediterranean alien inventories, unless records are based on a wide number of specimens. In particular, we here refer to: ✓ Antigona lamellaris Schumacher, 1817: based on a single shell from Turkey [START_REF] Engl | New migrant species from southeast Turkey Psammotreta praerupta (Salisbury, 1934) and Antigona lamellaris Schumacher[END_REF]; ✓ Canarium mutabile (Swainson, 1821): reported from Israel [START_REF] Mienis | Marine molluscs from the Eastern Mediterranean 5. first finds Canarium mutabilis[END_REF] on the basis of three empty shells; ✓ Cerithium litteratum (Born, 1778): only known by a single empty shell found in Greece in 1978 (Garilli and Vardala-Thedorou, 2005); ✓ Cerithium nesioticum Pilsbry and Vanatta, 1906: only known from empty shells from Israel [START_REF] Mienis | Cerithium nesioticum Pilsbry & Vanatta, 1906. Another Indo-Pacific species from the Mediterranean coast of Israel[END_REF] and Cyprus [START_REF] Bogi | La malacofauna dell' Isola di Cipro[END_REF]; ✓ Ergalatax contracta (Reeve, 1846): only known from empty shells [START_REF] Mienis | New data concerning the presence of Indo-Pacific molluscs in the Mediterranean Sea with emphasis on Israel[END_REF]; ✓ Haliotis rugosa pustulata Reeve, 1846: a few shells only in Israel and Libya (see [START_REF] Zenetos | CIESM Atlas of Exotic Species in the Mediterranean[END_REF]; ✓ Monetaria annulus (Linnaeus, 1758): [START_REF] Mienis | New data concerning the presence of Indo-Pacific molluscs in the Mediterranean Sea with emphasis on Israel[END_REF] [START_REF] Mienis | A record of Natica gualteriana, an Indo-Pacific species, from the Mediterranean coast of Israel[END_REF][START_REF] Mienis | A second record of Notocochlis gualteriana from the Mediterranean coast of Israel[END_REF]; ✓ Rissoina ambigua (Gould, 1849): a microspecies, whose records are based on few empty shells found in Turkey in 2003 [START_REF] Mienis | New data concerning the presence of Indo-Pacific molluscs in the Mediterranean Sea with emphasis on Israel[END_REF]; ✓ Trapezium oblongum (Linnaeus, 1758): only known by a single valve [START_REF] Mienis | A record of the indo-pacific species Trapezium oblongum from the Mediterranean coast of Israel[END_REF]. Its real occurrence in the Mediterranean Sea was considered doubtful by [START_REF] Mienis | A record of the indo-pacific species Trapezium oblongum from the Mediterranean coast of Israel[END_REF] himself. Finally, the following species are not established (species recorded once or twice): ✓ Acanthopleura sp.: few specimens from Tripoli (Lybia) and Zarzis (Tunisia), where the species is considered as casual (see [START_REF] Zaouali | Contribution a ' l'etudes peuplements actuels des substrats solides de l'etage mediolittoral de la Mediterranee meridionale[END_REF][START_REF] Crocetta | Biogeographical homogeneity in the eastern Mediterranean Sea e iii. New records and a state of the art of Polyplacophora, Scaphopoda and Cephalopoda (Mollusca) from Lebanon[END_REF][START_REF] Ounifi-Ben Amor | Update of alien fauna and new records from Tunisian marine waters[END_REF]; ✓ Acar plicata (Dillwyn, 1817): only known from a single record in Israel [START_REF] Barash | Further additions to the knowledge of Indo-Pacific Mollusca in the Mediterranean Sea[END_REF], as the additional record from Greece should be considered as questionable (see [START_REF] Corsini-Foka | Inventory of alien and cryptogenic species of the Dodecanese (Aegean Sea, Greece): Collaboration through COST action training school[END_REF]; ✓ Atactodea striata (Gmelin, 1791): only known from two single records - [START_REF] Barash | Additions to the knowledge of Indo-Pacific Mollusca in the Mediterranean[END_REF] and [START_REF] Cachia | The Marine Mollusca of the Maltese Islands. Part Four. The Classes: Caudofoveata, Solenogastres, Bivalvia, Scaphopoda & Cephalopoda[END_REF] [START_REF] Barash | Fauna Palaestina: Mollusca I. Annotated List of Mediterranean Molluscs of Israel and Sinai[END_REF]; ✓ Cuthona perca (Er. Marcus, 1958): only known from Italy on the basis of an old record of a single specimen [START_REF] Perrone | Una specie di Nudibranchi del genere Cuthona Alder & Hancock, 1855, nuova per il Mediterraneo: Cuthona perca Marcus, 1958 (Opisthobranchia: Nudibranchia)[END_REF]; ✓ Halgerda willeyi Eliot, 1904: only known from Croatia on the basis of an old record of a single specimen [START_REF] Turk | The opistobranch mollusks (Cephalaspidea, Saccoglossa, Notaspidea, Anaspidea and Nudibranchia) of the Adriatic Sea with special reference to the Slovenian coast[END_REF]; ✓ Lodderia novemcarinata (Melvill, 1906): only known from 5 specimens from a single locality (O € ztürk et al., 2015); ✓ Mercenaria mercenaria (Linnaeus, 1758): the Northern quahog cannot be classified as abundant, and not even as established.

Attempts were made to introduce the clam to the Etang de Thau (France), while the putative records from Italy were confuted early after [START_REF] Zenetos | CIESM Atlas of Exotic Species in the Mediterranean[END_REF], followed by the first concrete record of few specimens (see [START_REF] Turolla | Considerazioni sul ritrovamento di esemplari adulti di Mercenaria mercenaria (Linnaeus, 1758) in due lagune del Delta del Po[END_REF]. No populations have successfully been established anywhere in the Mediterranean;

✓ Pinctada margaritifera (Linnaeus, 1758): its potential introduction in Italy has been recently confuted (see discussions in [START_REF] Crocetta | Alien Mollusca along the Calabrian shores of the Messina Strait area and a review of their distribution in the Italian Seas[END_REF], so that it is only known on the basis of a single remaining record from Egypt [START_REF] Zenetos | CIESM Atlas of Exotic Species in the Mediterranean[END_REF]; ✓ Plocamopherus tilesii Bergh, 1877: only known from Turkey on the basis of a single specimen that was collected in 2009 [START_REF] Yokes | Alien Opisthobranchs from Turkish coasts: first record of Plocamopherus tilesii Bergh, 1877 from the Mediterranean[END_REF]; ✓ Spondylus nicobaricus Schreibers, 1793: only known from Israel on the basis of an old record of a single specimen [START_REF] Zenetos | CIESM Atlas of Exotic Species in the Mediterranean[END_REF]; ✓ Stomatella impertusa (Burrow, 1815): known from a single record from Turkey (see [START_REF] Zenetos | CIESM Atlas of Exotic Species in the Mediterranean[END_REF], and a possible, but still doubtful, record from Malta (see Arias et al., 2013b), where the species was not found again despite careful researches (FC, unpublished data); ✓ Teredothyra dominicensis (Bartsch, 1921): specimens from Turkey were predominantly acquired from a shipwreck off the coast of Kas. The Greek record should not be considered, as a single specimen was found on a piece of driftwood that was recovered off the coast of Fourni Island (Luisa Borges, pers. Comm.). Following [START_REF] Marchini | Recommendations on standardizing lists of marine alien species: lessons from the Mediterranean Sea[END_REF], the aforementioned records do not qualify for a successful establishment. 3.1.1.3. Polychaeta (7 þ 2). The following species must be omitted: Kinberg, 1865: most probably it could be E. vittata (see discussion in [START_REF] Ben-Eliahu | Errant polychaete cryptofauna (excluding Syllidae and Nereidae) from rims of similar intertidal vermetid reefs on the Mediterranean coast of Israel and in the Gulf of Elat[END_REF]; ✓ Hydroides dianthus (Verrill, 1873): according to a recent molecular study [START_REF] Sun | A global invader or a complex of regionally distributed species? Clarifying the status of an invasive calcareous tubeworm Hydroides dianthus (Verrill, 1873) (Polychaeta: Serpulidae) using DNA barcoding[END_REF], the global invader H. dianthus seems to be originated from the Mediterranean Sea. However, it was emphasised that specimens of Hydroides cf. dianthus in the Black Sea could have been introduced to the region from the West Coast of the USA; ✓ Linopherus incarunculata (Peters, 1854): this species has not been reported from the Mediterranean Sea. The only species reported was L. acarunculatus [START_REF] Monro | A note on a collection of Polychaeta from the eastern Mediterranean with the description of a new species[END_REF] that was later proved to be a misidentification of L. canariensis Langerhans, 1881 (see Çinar, 2009); ✓ Marphysa disjuncta Hartman, 1961: the species was re-identified as Marphysa cinari Kurt-Sahin 2014, a Mediterranean endemic species (Kurt Sahin, 2014); ✓ Neanthes willeyi (Day, 1934[START_REF] Fauvel | Contribution a la faune des Annelides Polyche 'tes des co ^tes d'Israel[END_REF] reported that N. willeyi was found in Port Said (as Nereis capensis), quoting his 1927 paper [START_REF] Fauvel | Rapport sur les Annelides Polyche 'tes Errantes[END_REF]. Indeed [START_REF] Fauvel | Rapport sur les Annelides Polyche 'tes Errantes[END_REF] found this species at one station in the Suez Canal just 5 km away from Port Said. [START_REF] Ben-Eliahu | Nereididae of the Suez CanalePotential Lessepsian migrants?[END_REF] considered this species as AS in the Mediterranean, based on the expression made by [START_REF] Fauvel | Contribution a la faune des Annelides Polyche 'tes des co ^tes d'Israel[END_REF].

✓ Eunice indica
As there is no true record of this species in the Mediterranean, this species should be excluded from the list; ✓ Onuphis eremita oculata Hartman, 1951: the record was reidentified as Onuphis eremita Audouin and Milne Edwards, 1833. [START_REF] Arias | Hidden diversity within the polychaete Onuphis eremita sensu lato (Annelida: Onuphidae)dredescription of O. eremita Audouin & Milne-Edwards, 1833 and reinstatement of Onuphis pancerii Clapare 'de, 1868[END_REF] stated 'Since the original description of O. eremita indicates the presence of eyespots, as well as that they are present in the neotype in most of the studied specimen, it is very likely that the subspecies reported by Çinar ( 2009) is actually O. eremita and hence is a Mediterranean native species'; ✓ Laonome elegans Gravier, 1906: it is a questionable species (see Ben-Eliahu, 1972, p. 232) In addition, the following species are not established (species recorded once or twice):

✓ Perinereis nuntia (Lamarck, 1818): the species was regarded as casual (Zenetos et al., 2010) as it was only reported from Port Said (Egypt) in 1924 by [START_REF] Fauvel | Rapport sur les Annelides Polyche 'tes Errantes[END_REF]. There is no subsequent record of this species in the Mediterranean; ✓ Branchiomma boholense (Grube, 1878): besides the record by [START_REF] Knight-Jones | Sabelliform polychaetes, mostly from Turkey's Aegean coast[END_REF], all other reports are questionable. The reports from the western Mediterranean were proved to be B. bairdi (McIntosh, 1885) (see [START_REF] Çinar | Alien polychaete species (Annelida: Polychaeta) on the southern coast of Turkey (Levantine Sea, eastern Mediterranean), with 13 new records for the Mediterranean Sea[END_REF][START_REF] Giangrande | Sabellidae (Annelida) from the Faro coastal lake (Messina, Ionian Sea), with the first record of the invasive species Branchiomma bairdi along the Italian coast[END_REF], as well as the reports from Cyprus (see Çinar, 2005).

3.1.1.4. Crustacea (1 þ 3).
The following species must be omitted:

✓ Nothobomolochus fradei Marque 's, 1965: reported as parasite on Herklotsichthys punctatus (Rüppell, 1837), which was caught in Egyptian waters off the Alexandrian coast in 2008 (El-Rashidy andBoxshall, 2009, 2010). It cannot be confirmed as an alien because of its natural distribution [which includes both the Arabian Gulf and the Eastern Atlantic coast (Gulf of Guinea)]. This widespread distribution indicates that it could be native in the Mediterranean, where it was also found on the native fish Sardina pilchardus (Walbaum, 1792) (G. Boxshall, pers. comm.); ✓ Bemlos leptocheirus (Walker, 1909): only known from a single record from Port Said [START_REF] Schellenberg | Report on the Amphipoda[END_REF] [START_REF] Monniot | Ascidies littorals de Guadelope. II. Phlebobranches[END_REF][START_REF] Monniot | Discussion sur la valeur de deux genres d'ascidies a ' propos d'espe 'ces nouvelles en mediterranee[END_REF]. It is different from Perophora viridis Verrill, 1871. Common synonyms are Perophora bermudensis Berrill, 1932 andPerophora formosana (Oka, 1931) [START_REF] Monniot | Discussion sur la valeur de deux genres d'ascidies a ' propos d'espe 'ces nouvelles en mediterranee[END_REF][START_REF] Kott | The Australian Ascidiacea. Part 1, Phlebobranchia and Stolidobranchia[END_REF]. If we accept these synonymies, then P. multiclathrata occurs in the Indo-Pacific, and on both sides of the tropical Atlantic. The Mediterranean reports, however, concern only two findings in the same locality (F. Monniot, pers. comm.), thus the species cannot be considered as presently established. Zibrowius, 2007[START_REF] Harmelin | High xenodiversity versus low native diversity in the south-eastern Mediterranean: bryozoans from the coastal zone of Lebanon[END_REF] considered this species as cryptogenic.

3.1.1.6. Bryozoa (1 þ 0). Schizoretepora hassi Harmelin, Bitar &
3.1.1.7. Echinodermata (0 þ 1). Ophiocoma scolopendrina (Lamarck, 1816): record based on a single specimen [START_REF] Zaouali | Contribution a ' l'etudes peuplements actuels des substrats solides de l'etage mediolittoral de la Mediterranee meridionale[END_REF].

3.1.1.8. Cnidaria/Anthozoa (1 þ 0). The colonial scleractinian Oculina patagonica de Angelis, 1908, was described from fossil material in South America. It is assumed to have been introduced in anthropogenic times into the Mediterranean, but as "nothing is known on the actual range of the species" [START_REF] Zibrowius | Oculina patagonica, Scleractiniaire hermatypique introduit en Mediterranee[END_REF]. However, genetic, historic, demographic, and fossil evidence suggests that O. patagonica has not been anthropogenically introduced into the Mediterranean from the western North Atlantic. Mediterranean and Atlantic populations are genetically distinct and appear to have begun diverging about 5 Mya [START_REF] Leydet | The invasive coral Oculina patagonica has not been recently introduced to the Mediterranean from the western Atlantic[END_REF].

Results support the hypothesis that Oculina has had a long history in the eastern Atlantic, but remained undetected until it recently began expanding to invasive numbers in the Mediterranean, likely in response to environmental changes [START_REF] Leydet | The invasive coral Oculina patagonica has not been recently introduced to the Mediterranean from the western Atlantic[END_REF].

3.1.1.9. Fish (0 þ 7). Seven species must be omitted as not established:

✓ Sciaenops ocellatus (Linnaeus, 1766): aquaculture escapee. Only two specimens have been collected to date. This species does not reproduce in the wild in the Mediterranean [START_REF] Golani | Records of Paralichthys lethostigma and Sciaenops ocellatus in the Mediterranean and Chana micropeltes in Lake Kinneret (Sea of Galilee), Israel[END_REF]; ✓ Chanos chanos (Forsskål, 1775) [START_REF] Sami | First record of Pampus argenteus (Euphrasen, 1788) (Osteichthyes: Stromateidae) in Tunisian coast (Mediterranean sea)[END_REF] with no follow up, cannot account for establishment of the species; ✓ Tridentiger trigonocephalus (Gill, 1859): only one specimen has been collected. However, the people that collected the specimen claimed that they saw several specimens in the same location that looked similar to this species (DG, unpublished). With the lack of concrete evidence, the species is classified as nonestablished; ✓ Abudefduf vaigiensis (Quoy and Gaimard, 1825): the species that occurs in the Levant is Abudefduf saxatilis (see: [START_REF] Tsadok | On the occurrence and identification of Abudefduf saxatilis (Linnaeus, 1758) in the easternmost Mediterranean Sea[END_REF], therefore, it obviously did not arrive via the Suez Canal and it is not rare. However, recently [START_REF] Vella | The first record of the lowfin chub Kyphosus[END_REF] claim that in their genetic study they found that both A. vaigiensis and A. saxatilis co-exist in Malta.

Additional species

Annex 1 includes 72 established aliens in the Mediterranean that are missing in [START_REF] Galil | East is east and West is west? Management of marine bioinvasions in the Mediterranean Sea[END_REF]. Among the taxa, most of the macroalgae are included in the recent CIESM atlas [START_REF] Verlaque | Macrophytes[END_REF], while 4 species, namely Monosporus indicus Børgesen, Dictyota cyanoloma Tronholm, De Clerck, Gomez Garreta & Rull Lluch, Cutleria multifida (Turner) Greville and Halimeda incrassata (J. Ellis) J. V. Lamouroux were reported in 2016 [START_REF] Hoffman | Tetrasporangial plants of Monosporus indicus (Ceramiales, Rhodophyta): a new alien in the Mediterranean Sea[END_REF][START_REF] Aragay | Phenological and molecular studies on the introduced seaweed Dictyota cyanoloma (Dictyotales, Phaeophyceae) along the Mediterranean coast of the Iberian Peninsula[END_REF][START_REF] Kawai | Phylogeographic analysis of the brown alga Cutleria multifida (Tilopteridales, Phaeophyceae) suggests a complicated introduction history[END_REF][START_REF] Alos | Fast-spreading green beds of recently introduced Halimeda incrassata invade Mallorca island (NW Mediterranean Sea)[END_REF]. Previously considered as debatable [START_REF] Verlaque | Macrophytes[END_REF], the introduction of the Red Sea species Palisada mariserubri (K.W. Nam & Saito) K.W. Nam in the Mediterranean has been confirmed by reports from Greece [START_REF] Tsiamis | New Mediterranean biodiversity records (July 2015)[END_REF] and Tunisia [START_REF] Boudouresque | The Red Sea macroalga Palisada maris-rubri (Rhodobionta, Archaeplastida): first record in Tunisia[END_REF].

The molluscan aliens are adequately covered. Exceptions are: i) Indothais sacellum (Gmelin, 1791), a species first recorded in 2000 as very common in the Beyrouth Harbour, Lebanon [START_REF] Zibrowius | Invertebres marins exotiques sur la co ^te du Liban[END_REF]. The species is also spreading in other Mediterranean countries, such as Israel, where it was found in at least in two localities [START_REF] Mienis | Marine molluscs from the Eastern Mediterranean 26. The first finds of Thais sacellum (Gmelin, 1791) in Israel[END_REF]; ii) Fossarus eutorniscus Melvill, 1918, a taxon that was first reported in the Mediterranean Sea as Parviturbo dibellai [START_REF] Buzzurro | I molluschi lessepsiani di Tasucu (Turchia sudorientale): descrizione di Parviturbo dibellai n. sp. (Gastropoda: Trochoidea: Skeneidae)[END_REF] on the basis of a wide number of shells from Israel and Turkey [START_REF] Buzzurro | I molluschi lessepsiani di Tasucu (Turchia sudorientale): descrizione di Parviturbo dibellai n. sp. (Gastropoda: Trochoidea: Skeneidae)[END_REF], and subsequently assigned to the Lessepsian species F. eutorniscus [START_REF] Rubio | Revision of the genera Parviturbo vaigiensis (Quoy & Gaimard, 1825) from Malta[END_REF]. In addition, [START_REF] Galil | East is east and West is west? Management of marine bioinvasions in the Mediterranean Sea[END_REF] excluded "species with unresolved taxonomic status (species complex or morphologically similar taxa)". This approach presumably led to the exclusion of Dendostrea cf. folium from the species list, although this taxon is clearly an AS in the Mediterranean Sea and only its specific attribution is problematic due to the absence of molecular studies that aim to solve the Indo-Pacific Dendostrea complex of species (Crocetta et al., 2015a).

The majority of polychaetes are included in the reviews by Zenetos et al. (2010[START_REF] Zenetos | Alien species in the Mediterranean Sea by 2012. A contribution to the application of European Union's Marine strategy framework directive (MSFD). Part 2. Introduction trends and pathways[END_REF] and Çinar (2013). The most recently introduced species is Diopatra marocensis Paxton, Fadlaoui & Lechapt, 1995, which was reported from different localities along the Levant and Aegean coasts of Turkey (Çinar et al., 2014a) and might have been introduced to the area via the ballast water of ships. Furthermore, worth mentioning are: ✓ Prionospio pulchra Imajima 1990: first reported from the Greek coast of the Aegean Sea by [START_REF] Pancucci-Papadopoulou | Update of marine alien species in Hellenic waters[END_REF] and subsequently reported from Levantine, Sea of Marmara and Black Sea coasts by [START_REF] Dagli | Species of the subgenus Minuspio (Polychaeta: Spionidae: Prionospio) from the southern coast of Turkey (Levantine Sea, eastern Mediterranean), with the description of two new species[END_REF]. However, the specimens that were collected on the Italian coast of the Tyrrhenian Sea in 1982 and identified as P. cirrifera Wiren, 1883 by [START_REF] Giangrande | Distribuzione dei Policheti nei fondi mobili della Rada di Augusta (Sicilia). Boll[END_REF] were re-identified as P. pulchra [START_REF] Dagli | Species of the subgenus Minuspio (Polychaeta: Spionidae: Prionospio) from the southern coast of Turkey (Levantine Sea, eastern Mediterranean), with the description of two new species[END_REF]; ✓ Timarete punctata (Grube, 1859), which was possibly confused with T. semicincta (Ehlers, 1905) identified from the Lebanon coast in 1965 [START_REF] Laubier | Sur quelques Annelides Polyche 'tes de la region de Beyrouth[END_REF], occurred abundantly in harbour environments on the southern coast of Turkey (Çinar, 2007); ✓ Chaetozone corona Berkeley and Berkeley, 1941: the species was initially reported as cryptogenic in the Mediterranean Sea [START_REF] Çinar | The presence of Chaetozone corona (Polychaeta: Cirratulidae) in the Mediterranean Sea: an alien or a native species? Cah[END_REF], but it was later reported from the eastern Atlantic coast as an AS that was introduced by shipping from the East Coast of the USA (Le [START_REF] Garrec | Chaetozone corona (Polychaeta, Cirratulidae) in the Bay of Biscay: a new alien species for rhe north-east Atlantic waters[END_REF]. Therefore, the species can be also considered as an established alien species in the Mediterranean Sea; ✓ Loimia medusa (Savigny, 1818) was mentioned as questionable in Zenetos et al. (2010). However, there is growing evidence that this species occurs in the eastern Mediterranean and can be regarded as an established AS [START_REF] Dorgham | The role of alien polychaetes along the Alexandria Coast, Egypt[END_REF]MEÇ pers. observ.). The first record of this species was given by [START_REF] Rullier | Les Annelides polyche 'tes du Bosphore, de la Mer de Marmara et de la Mer Noire, en relation avec celles de la Mediterranee[END_REF] in the Sea of Marmara. In the Mediterranean basin, L. medusa was included in species lists from Greece (collection date: 1976) [START_REF] Bogdanos | The macrozoobenthos of an Aegean embayment[END_REF] and Italy (collection date: 1987) [START_REF] Albertelli | Macrobenthic assemblages in Pelagie Islands and Pantelleria (Ionian Sea, Mediterranean)[END_REF]; ✓ Eurythoe complanata (Pallas, 1766): whose first report from the Mediterranean Sea (Israeli coast) was given by [START_REF] Monro | A note on a collection of Polychaeta from the eastern Mediterranean with the description of a new species[END_REF]. The species was subsequently reported from different parts of the Mediterranean Sea, including Gibraltar (see Arias et al., 2013a). As Çinar (2008) postulated, unless the taxonomic status of the Eurythoe species, E. syriaca Kinberg, 1857 and E. hedenborgi Kinberg, 1857, which were originally described from the eastern Mediterranean by [START_REF] Kinberg | Nya sla €gten och arter af Annelider. O € fversigt af Ko €niglich Vetenskapsakademiens fo €rhandlingar[END_REF] and subsequently considered as a junior synonyms of E. complanata or indeterminable by different authors, are solved, it is hard to determine the status and first arrival time of E. complanata in the Mediterranean Sea. However, as types of E. syriaca and E. hedenborgi are presumably lost and their original descriptions were too short and without figures, we should assume, for the time being, that the taxonomic status of these species are nomen dubium. Therefore, we can conclude that E. complanata occurs in the region and is an established alien species; ✓ Eurythoe laevisetis Fauvel, 1914 was first reported from the Chafarinas Island (Alboran Sea) and Malta in the Mediterranean Sea, and was considered as an established alien species (Arias et al., 2013a). However, as this species was originally described from the eastern Atlantic (Gulf of Guinea) and known also from the Canary Island (Arias et al., 2013a), this species might have entered into the Mediterranean by the process of range expansion rather than by human assistance. However, there is a gap in the distribution of the species within the Mediterranean, which is absent in the westernmost part. Therefore, this species should be kept in the list as an established alien species until more data about its distributional pattern in the western Mediterranean are accumulated that would enable us to realise the pathway through which it has become established in Maltese waters; natural dispersal via Gibraltar or shipping? Crustacea are also successfully covered. In addition to the two Cumacean species Eocuma rosae Corbera and Galil, 2007 and Eocuma sarsii (Kossmann), 1880 that are reported as established in [START_REF] Corbera | Cumacean assemblages on the Levantine shelf (Mediterranean Sea)espatiotemporal trends between 2005 and 2012[END_REF], the copepod Parvocalanus crassirostris (Dahl F., 1894), an old record from Egypt [START_REF] Gurney | Report on the Crustacea. Copepoda (littoral and semiparasitic). Zoological results of the Cambridge expedition to the Suez Canal[END_REF], which was recently found in the Adriatic [START_REF] Vidjak | First record of small tropical calanoid copepod Parvocalanus crassirostris (Copepoda, Calanoida, Paracalanidae) in the Adriatic Sea[END_REF] is missing. The amphipod Linguimaera caesaris Krapp-Schickel, 2003 has been mistakenly deleted from earlier lists and reported as Hamimaera hamigera (Haswell, 1879) (Zenetos et al., 2011a).

With regards to alien ascidians the list is enriched with the addition of nine species:

✓ Aplidium accarense (Millar, 1953) [START_REF] Monniot | Microcosmus anchylodeirus (Ascidiacea, Pyuridae) introduced in the Mediterranean Sea[END_REF]. The donor region and the introduction pathway of this western-Atlantic species are unknown; ✓ Polyclinum constellatum Savigny, 1816: Halim and[START_REF] Halim | Aliens in Egyptian waters. A checklist of ascidians of the Suez Canal and the adjacent Mediterranean waters. Egypt[END_REF] report this species from the area of Port Said; ✓ Styela clava Herdman, 1881: although the first record in Egypt [START_REF] Ghobashy | Ascidians in Egyptian waters[END_REF] is unsubstantiated (insufficient description), the species was reported from France [START_REF] Davis | First record of Styela clava (Tunicata, Ascidiacea) in the Mediterranean region[END_REF]; first seen in 2004), and now is spreading into the Mediterranean (Çinar, 2016).

Four Bryozoa species should be added. The vast majority of them were reported in Lebanon, after the inventories of [START_REF] Zenetos | Annotated list of alien marine species in the Mediterranean with emphasis on worst invasive species[END_REF][START_REF] Zenetos | Alien species in the Mediterranean Sea by 2012. A contribution to the application of European Union's Marine strategy framework directive (MSFD). Part 2. Introduction trends and pathways[END_REF]. To be more precise, [START_REF] Harmelin | Alien bryozoans in the eastern Mediterranean Seadnew records from the coast of Lebanon[END_REF] and [START_REF] Harmelin | High xenodiversity versus low native diversity in the south-eastern Mediterranean: bryozoans from the coastal zone of Lebanon[END_REF], based on collections made some years earlier, reported 27 AS from Lebanon. Among them, two are cryptogenic and others such as Thalamoporella rozieri (Audouin, 1826), and Celleporaria vermiformis (Waters, 1909) cannot be included in the present inventory because they were recorded from a single site. In contrast, other species seem to be widely distributed in the Levantine Sea as they have also been recorded along the coasts of Israel by [START_REF] Sokolover | Bryozoa from the Mediterraneancoast of Israel[END_REF].

✓ Celleporina bitari [START_REF] Harmelin | Alien bryozoans in the eastern Mediterranean Seadnew records from the coast of Lebanon[END_REF] this species has been recorded in samples that were collected in 2002 and 2003 in Lebanon. Also known from the Red Sea, C. bitari has been considered by [START_REF] Harmelin | Alien bryozoans in the eastern Mediterranean Seadnew records from the coast of Lebanon[END_REF] as introduced into the Mediterranean via Suez either through shipping or colonising successive relays along the Canal; ✓ Celleporaria cf. sherryae Winston, 2005: this species has been reported from Lebanon by [START_REF] Harmelin | Alien bryozoans in the eastern Mediterranean Seadnew records from the coast of Lebanon[END_REF] and is considered as an alien bryozoan in the Mediterranean by [START_REF] Harmelin | High xenodiversity versus low native diversity in the south-eastern Mediterranean: bryozoans from the coastal zone of Lebanon[END_REF] who postulate its introduction through Gibraltar via long-distance vessels; ✓ Microporella genisii (Audouin, 1826): this species has been reported from the coasts of Lebanon, predominantly in natural habitats on various substrata and in a wide depth range (5e40 m); ✓ Mucropetraliella thenardii (Audouin, 1826): this species has been reported from the coast of Lebanon [START_REF] Harmelin | Alien bryozoans in the eastern Mediterranean Seadnew records from the coast of Lebanon[END_REF][START_REF] Harmelin | High xenodiversity versus low native diversity in the south-eastern Mediterranean: bryozoans from the coastal zone of Lebanon[END_REF] and from some localities along the coasts of Israel by [START_REF] Sokolover | Bryozoa from the Mediterraneancoast of Israel[END_REF].

With regards Cnidaria, molecular analyses of the widely distributed jellyfish Aurelia, have revealed the presence of Aurelia coerulea von Lendenfeld, 1884 and Aurelia solida Browne, 1905, as established AS in the Mediterranean Sea [START_REF] Scorrano | Unmasking Aurelia species in the Mediterranean Sea: an integrative morphometric and molecular approach[END_REF]. The firework anemone Pachycerianthus multiplicatus Carlgren, 1912 was reported from I _ skenderun Bay based on material collected in 2009 and might have been introduced to the area by ballast waters of vessels (Çinar et al., 2014b).

The flatworm Maritigrella fuscopunctata (Prudhoe, 1978) was reported from Malta in 2015 but it was known from Israel and Lebanon since 2014 (Portelli et al. in Crocetta et al., 2015a,b).

The sipunculan Aspidosiphon elegans (Chamisso and Eysenhardt, 1821) that was reported by Wesenberg-Lund, 1957, appears to be established in the Levantine Sea (Turkey: [START_REF] Açik | Sipuncula from the southern coast of Turkey (eastern Mediterranean), with a new report for the Mediterranean Sea[END_REF].

Finally in this revised list, three fish species are added: [START_REF] Fricke | First record of the Indian anchovy Stolephorus indicus (van Hasselt, 1823) (Clupeiformes: Engraulidae) in the Mediterranean Sea[END_REF].

✓
The information gaps, the lack of previous records, as well as difficulties in taxonomic identification and general confusion in species global biogeography and species level biodiversity makes it difficult for some groups to decide on categorising them as alien or cryptogenic. This is a common problem in alien marine species which will be only solved with molecular studies. Here are some examples of established species that are presently not included in our list as there is not enough information on taxonomic status, introduction vector, cryptic diversity, etc. These examples are best labelled as cryptogenic species until more information is available.

The ascidian Clavelina lepadiformis (Müller, 1776). [START_REF] Turon | Characterising invasion processes with genetic data: an Atlantic clade of Clavelina lepadiformis (Ascidiacea) introduced into Mediterranean harbours[END_REF] show that the form living inside ports in the Mediterranean is likely introduced from the Atlantic. But, at present, although the forms living inside and outside of ports are considered as two clades of a single species, no formal taxonomic distinction has been made. The earliest record that specifically mentioned C. lepadiformis inside ports in the Mediterraenan is Della Valle (1877) in Naples, and the introduction vector is likely via vessels.

The ascidian Perophora viridis Verrill, 1871 is common in the West Atlantic Ocean, where it was described. The species is present also in the Pacific Ocean. In the Mediterranean P. viridis was signaled in 1921 by Neppi (under the synonym P. dellavallei) in Naples, then by [START_REF] Brunetti | Ascidians of the Laguna Veneta II. Distribution and ecological observations[END_REF] in Venice, and by [START_REF] Monniot | Discussion sur la valeur de deux genres d'ascidies a ' propos d'espe 'ces nouvelles en mediterranee[END_REF] in Corsica. It is abundant in the Iberian Mediterranean [START_REF] Turon | Estudio de las ascidias de las costas de Catalun ~a e Islas Baleares[END_REF][START_REF] Ramos-Espla | Ascidias litorales del Mediterraneo Iberico. Faunística, ecología y biogeografía[END_REF]. Although it is sometimes labelled as alien, we consider P. viridis as cryptogenic. This species may be abundant in the Atlanto-Mediterranean area but has been confused with Perophora listeri Wiegman, 1835. Nowadays, although P. listeri and P viridis are accepted as different species, it is impossible to disentangle to which species old reports are referring to. It seems difficult to consider it to be introduced if it is abundant and widely distributed in non-artificial habitats.

The golden star tunicate Botryllus schlosseri (Pallas, 1766) is distributed worldwide and has a marked polymorphism in chromatic patterns and colony shapes. As in other widely distributed and polymorphic species, it has been recently shown that the taxon B. schlosseri is a complex of five genetically differentiated species [START_REF] Lopez-Legentil | Genetic structure of the star sea squirt, Botryllus schlosseri, introduced in southern European harbours[END_REF][START_REF] Bock | Multilocus genetic analyses differentiate between widespread and spatially restricted cryptic species in a model ascidian[END_REF], and all of them present in the Mediterranean. The most widespread of them is the so-called Clade A, which in turn is subject to ongoing speciation processes [START_REF] Griggio | Ascidian mitogenomics: comparison of evolutionary rates in closely related taxa provides evidence of ongoing speciation events[END_REF]. It is impossible, in retrospect, to assign previous reports of the species complex to any of its true genetic species. In addition, it is not clear which is the native area of the invasive Clade A [START_REF] Lejeusne | Comparative phylogeography of two colonial ascidians reveals contrasting invasion histories in North America[END_REF][START_REF] Ruit Nydam | Origin and dispersal history of two colonial ascidian clades in the Botryllus schlosseri species complex[END_REF][START_REF] Reem | Phylogenetics, biogeography and population genetics of the ascidian Botryllus schlosseri in the Mediterranean Sea and beyond[END_REF]. It is therefore advisable to leave the status of B.schlosseri in the Mediterranean as cryptogenic, for the time being.

The bryozoan Bugula neritina (Linnaeus, 1758) has belonged to the Mediterranean fauna for a very long time. Recent molecular data [START_REF] Fehlauer-Ale | Cryptic species in the cosmopolitan Bugula neritina complex (Bryozoa, Cheilostomata)[END_REF] have shown that B. neritina is a complex of three cryptic species, but unfortunately Mediterranean populations were not considered in that study [START_REF] Harmelin | High xenodiversity versus low native diversity in the south-eastern Mediterranean: bryozoans from the coastal zone of Lebanon[END_REF].

The bryozoan Scorpiodinipora costulata (Canu and Bassler, 1929). Considering the broad allopatric distribution of this morphotype across the oceans and the low capacity of dispersal of species with short-lived larvae, it is likely that this material includes several sibling species.

The bryozoan Microporella harmeri Hayward, 1988. It is reported from the coast of Lebanon [START_REF] Harmelin | Smittinidae (Bryozoa, Cheilostomata) from coastal habitats of Lebanon (Mediterranean sea), including new and nonindigenous species[END_REF][START_REF] Harmelin | High xenodiversity versus low native diversity in the south-eastern Mediterranean: bryozoans from the coastal zone of Lebanon[END_REF] and Israel [START_REF] Sokolover | Bryozoa from the Mediterraneancoast of Israel[END_REF]. The species is distributed from the Atlantic to the Red Sea and the Indian Ocean, with populations from the different areas showing a series of slightly different "morphs" for several characters. Therefore, although it is listed in [START_REF] Zenetos | Alien species in the Mediterranean Sea by 2012. A contribution to the application of European Union's Marine strategy framework directive (MSFD). Part 2. Introduction trends and pathways[END_REF] and appears to be established, it is very difficult to say with certainty whether it belongs to a complex of cryptogenic species.

Abudefduf saxatilis (Linnaeus, 1758) is an Atlantic fish that is established in the Mediterraneanea Sea, but the pathway of its introduction remains debatable. The first record of this species in the Mediterranean is from the Gulf of Naples [START_REF] Tardent | Capture d'un Abudefduf saxatilis vaigiensis Q. und G. (Pisces, Pomacentridae) dans le Golfe de Naples[END_REF] and the second record (as A. vaigiensis) is from the Levantine basin [START_REF] Goren | First record of the Indo-Pacific, coral reef fish Abudefduf vaigiensis (Quoy & Gaimard, 1825) in the Levant[END_REF]. These facts indicate that the species has increased its range from West to East, after penetration via Gibraltar. Yet, the situation concerning Abudefduf spp. in the Mediterranean is complicated. Both A. saxatilis and A. vaigensis have often been found in places with shipping activities (see for example Malta and Genoa) or aquarium releases are likely (see Palamos in Spain, where further aquarium releases have been recently detected). Mediterranean findings of these species are separated by large distances and no clear geographical pattern can be highlighted. The small size of Abudefduf spp. would also fit with the idea of direct human transportation (E. Azzuro, pers. Comm.). However, as natural range expansion cannot be excluded, we have not included A. saxatilis in our list.

Kyphosus spp.: two, perhaps three, Kyphosus species-K. bigibbus Lacepe 'de, 1801, K. sectatrix (Linnaeus, 1758) and K. vaigiensis (Quoy and Gaimard, 1825) have been occasionally recorded in the Mediterranean. These species occur both in the Atlantic and Indo-Pacific regions, but it is likely that they entered the Mediterranean through the Strait of Gibraltar. However, it is unclear whether they have established reproductive native populations in the Mediterranean [START_REF] Mannino | An additional record of Kyphosus vaigiensis (Quoy & Gaimard, 1825) (Osteichthyes, Kyphosidae) from Sicily clarifies the confused situation of the Mediterranean kyphosids[END_REF].

TAXONOMIC/NOMENCLATURAL issues

Nomenclatural discrepancies were noted in 26 cases (Table 1). Following a major revision of Bivalvia [START_REF] Huber | Compendium of Bivalves 2. A Full-color Guide to the Remaining Seven Families[END_REF], Mollusca is the taxonomic group with the most nomenclatural changes. For example, two Timoclea species are known as Mediterranean immigrants: Timoclea djiboutiensis (F. P. Jousseaume, 1894), so far only known from Tunisia, and Timoclea roemeriana (Issel, 1869) only known from the Eastern Mediterranean, but not Timoclea marica (Linnaeus, 1758) that does not even occur in the Red Sea [START_REF] Huber | Compendium of Bivalves 2. A Full-color Guide to the Remaining Seven Families[END_REF].

The following species are alien species but have doubtful taxonomic entities: Amphioctopus cf. aegina (Gray, 1849), Acanthopleura sp.; Botrytella "cf. parva". C. brunnea (Hincks, 1884) found in 2004 in Turkey (as reported in [START_REF] Galil | East is east and West is west? Management of marine bioinvasions in the Mediterranean Sea[END_REF] is probably Celleporaria sp.

aff. brunnea and was found in Lebanon [START_REF] Harmelin | Alien bryozoans in the eastern Mediterranean Seadnew records from the coast of Lebanon[END_REF].

Based on additional morphological and a phylogenetic data, Pelagia benovici Piraino, Aglieri, Scorrano & Boero, 2014, a jellyfish recently described from the Northern Adriatic, has been assigned to the genus Mawia [renamed as Mawia benovici (Piraino, Aglieri, Scorrano and Boero, 2014) [START_REF] Avian | Redescription of Pelagia benovici into a new jellyfish genus, Mawia, gen. nov., and its phylogenetic position within Pelagiidae (Cnidaria: Scyphozoa: Semaeostomeae)[END_REF]].

Changes in establishment success

The terminology "Common, Local, Abundant, Rare" that is used to describe the establishment success may be misleading. Some species, which are reported as local, are in fact well established and widespread in the Mediterranean, or they may be local, yet abundant. Often, introduced ascidians have been reported from a few places, but in these places they can be tremendously abundant. In our list, we consider the establishment success not only in the country where they were first detected, but throughout the Mediterranean. Several examples of noted discrepancies are discussed here. Among macrophytes, Lithophyllum yessoense Foslie is well established and common in the Thau Lagoon and Sargassum muticum (Yendo) Fensholt can be classified as common/abundant (invasive) in several Mediterranean lagoons (MV, pers. observ.). Scytosiphon dotyi M.J.Wynne is established in Italy (Northern Adriatic); France, Spain and Turkey [START_REF] Verlaque | Macrophytes[END_REF]. Codium fragile subsp. fragile (Suringar) Hariot is established and abundant (localy invasive) in the Mediterranean [START_REF] Verlaque | Macrophytes[END_REF].

The bivalve Septifer cumingii Recluz, 1849, the bryozoan Arbopercula tenella (Hincks, 1880), and the echinoderm Amphiodia (Amphispina) obtecta Mortensen, 1940 should be all listed as common.

Among polychaetes, Pseudonereis anomala Gravier, 1900 is an invasive AS, widely distributed in the Mediterranean Sea, from all Levant countries to Izmir and Sicily [START_REF] Çinar | Lessepsian migrants expanding their distributional ranges; Pseudonereis anomala (Polychaeta: Nereididae) in Izmir Bay (Aegean Sea)[END_REF]; ) in shallow-water benthic habitats along the Levantine coasts and presumably is in competition with the native neredid species, such as Perinereis cultrifera (Grube, 1840) and Platynereis dumerilii (Audouin and Milne Edwards, 1834) [START_REF] Çinar | A preliminary study on population characteristics of the Lessepsian species, Pseudonereis anomala (Polychaeta: Nereididae), in Iskenderun Bay (Levantine Sea, eastern Mediterranean)[END_REF]. Branchiomma bairdi is a common and abundant AS in the Eastern and Western Mediterranean Sea, reaching up to 50 ind.m -2 in the harbour environment (Arias et al., 2013b) and coastal lakes [START_REF] Giangrande | Sabellidae (Annelida) from the Faro coastal lake (Messina, Ionian Sea), with the first record of the invasive species Branchiomma bairdi along the Italian coast[END_REF]. Among ascidians, Didemnum vexillum Kott 2002 was first reported in the Venice Lagoon [START_REF] Tagliapietra | First record of the colonial ascidian Didemnum vexillum Kott, 2002 in the Mediterranean: lagoon of Venice (Italy)[END_REF] and later in the Ebro Delta (Spain, Ordon ~ez et al., 2015). In these localities the species was highly abundant and has become a pest on bivalve cultures. It can be considered a species in expansion in the Mediterranean, a worrisome perspective given the harmful effects of this species in other areas (Lambert, 2009, and references therein). Herdmania momus [START_REF] Savigny | Memories sur les animaux sans ve 'rtebres[END_REF] should be also listed as common [START_REF] Shenkar | The solitary ascidian Herdmania momus: native (Red Sea)versus non-indigenous (Mediterranean) populations[END_REF].

Among Cnidaria, Sertularia marginata (Kirchenpauer, 1864) seems to be widespread (Egypt, Israel, Lebanon, Syria, Turkey, Greece, and Morocco, see [START_REF] Gonzalez-Duarte | Sertularia marginata (Cnidaria: Hydrozoa) in the Mediterranean: an alien species in expansion?[END_REF].

Among fishes, Terapon puta Cuvier, 1829 and Pomadasys stridens (Forsskål, 1775) have become prevalent on the Israeli coast in the last few years (Golani, unpublished data). Moreover, Decapterus russelli (Rüppell, 1830), Pelates quadrilineatus (Bloch, 1790), Torquigener flavimaculosus Hardy and Randall, 1983, Hippocampus fuscus Rüppell, 1838 and Ostorhinchus fasciatus (White, 1790) are common and widespread in the Levantine basin (see EASIN). Pterois miles (Bennett, 1828), not only is non-rare but has become invasive in the Levantine basin (Lebanon, Turkey, Cyprus, and Greece: Crocetta et al., 2015b;[START_REF] Kletou | A lionfish (Pterois miles) invasion has begun in the Mediterranean Sea[END_REF][START_REF] Dailianis | New Mediterranean biodiversity records (July 2016)[END_REF].

To avoid misconception in defining the degree of establishment of a species, it is suggested to use the terminology presented in [START_REF] Blackburn | A proposed unified framework for biological invasions[END_REF]. This hierarchical classification allows expression of the range of establishments from a casual record through to full naturalisation.

First observation details

Year and country of first record

Most of the disagreements on the first recipient country coincide with disagreement on the year of first sighting. Thus, out of 53 corrections that were spotted in the first recipient country, 48 are also attributed to deviation in the introduction date. Most discrepancies are due to quoting the publication date, and not looking for the exact collection date in the papers (i.e. [START_REF] Moazzo | Mollusques testaces marins du Canal de Suez[END_REF]/or not carefully reading the paper e.g. for Bryozoans in [START_REF] Harmelin | Smittinidae (Bryozoa, Cheilostomata) from coastal habitats of Lebanon (Mediterranean sea), including new and nonindigenous species[END_REF]. Some discrepancies are due to the fact that they are based on the first publication, while the first collection date was reported later. This applies for instance to the decapod Actaea savignii (H. Milne Edwards, 1834) first found in 2006 in Lebanon (Crocetta and Bariche in Crocetta et al., 2015b), but not in 2010 in Israel [START_REF] Karhan | First Mediterranean record of Actaea savignii (H. Milne Edwards, 1834) (Crustacea: Decapoda: Brachyura: Xanthidae), an additional Erythraean alien crab[END_REF]; The macroalgae Cladophora patentiramea (Montagne) Kützing was first found in 1991 in Cyprus, Lomentaria hakodatensis Yendo in 1978 in France, and Uronema marinum Womersley in 1989 in France [START_REF] Verlaque | Macrophytes[END_REF]. Another sound case is that of the invasive macroalga Caulerpa cylindracea Sonder. The correct year and country of first observation is 1985 in Tunisia and not 1990 in Libya [START_REF] Hamza | New mentions of Caulerpa racemosa (Forsskål) J. Agardh in the Gabe 's Gulf (Tunisia)[END_REF][START_REF] Sghaier | Review of alien marine macrophytes in Tunisia[END_REF]. In addition to such inaccurate accounts, a plethora of missing resources was noted from national lists and databases (e.g. MAMIAS, ELNAIS). For example, many records in ELNAIS (Zenetos et al., 2015) were ignored. As an example the bivalve molluscs Pseudochama corbierei (Jonas, 1846) has been included in ELNAIS with its source and introduction date. However, the introduction date was ignored. The following cases are noteworthy for crustaceans: ✓ Amphibalanus reticulatus (Utinomi, 1967): was first sighted in 2003 in Israel. The French record in 1977 was not in the wild, but relates to specimens found on the keel of a ship that sailed from the Indian Ocean to Toulon Harbour (France) through the Red Sea; ✓ Plagusia squamosa (Herbst, 1790): was first found in 1968 in Lebanon. The 1873 record of this species in France was from a ship hull. Traustedt 1883 mentions it in the Naples Gulf with the name Styela plicata (but, he lists Styela gyrosa of Heller as a synonym).

Finally, a few date changes are attributed to direct communication with the authors. Among them. Monotygma fulva (A. Adams, 1853) was corrected according to pers. comm by J.J.Van Aartsen; Septifer cumingii Recluz, 1849, according to pers. comm. by S. Albayrak.

The list published by [START_REF] Galil | East is east and West is west? Management of marine bioinvasions in the Mediterranean Sea[END_REF] includes 124 mistakes out of 614 records (20%) in first country and/or collection date, 9 of which refer to non-established (NE) species (Table 2). Details per taxonomic group are in Table 2. Most discrepancies were noticed in Mollusca (36 established, 3 non established) and Macroalgae (27 establishedþ2 non established), followed by Crustacea (13 estab-lishedþ1 non established); Fish (12 establishedþ2 non established), and Polychaeta (13), Ascidiacea (4 establishedþ1 non established), Bryozoa (3), other invertebrates (7). Consequently, it is inevitable to question the accuracy of data that the authors claim and the conclusions derived from those data.

Trends in introduction

The trend in new AS is used as a proxy of the trends of the invasive AS (IAS) in marine environments. Any increasing trend in the presence and abundance of AS in a given ecosystem, independently of their real impact, should be qualified as negative, whereas negative trends or stable situations, even if the environmental status cannot be defined as positive could be considered, at least, as acceptable. Any definition of Good Environmental Status (GES) in reference to descriptor 2 should be linked to the achievement of the GES in the biodiversity descriptors, in such a way that the environmental status in relation to AS would be defined as negative, if it is also negative for these other descriptors, and vice-versa (EEA, 2015). In our study, trend in new AS introductions was calculated on a decadal basis. We considered the decades of first introductions because the first observations have been mostly accidental.

Recording a new AS requires either targeted research or the collaboration of an observer with a taxonomist. Consequently, the first sighting of an AS in a region (date and place) has often nothing to do with the true date and the place of its first introduction. Therefore, the exact date of the first observation is of low interest in setting the post-introduction dynamics, because the decade is usually sufficient.

By 1950, it is estimated that 145 AS had been introduced ino the Mediterranean, 127 of which are established today. Fig. 1 demonstrates an increase in the rate of introductions after 1950 (sum of established and non-established species), which climbed to 17 species per year in the 2001e2010 period and decreased after 2010. This latter could be partly attributed to a time lapse between the observation of the latest finding and publication. At subregional level, in 2015 it was estimated that the rate of new introductions of AS during the period 2001e2010 was highest in the Aegean-Levantine Sea (~17 species per year), followed by the Ionian Sea and the Central Mediterranean Sea (~8 species per year), and the Western Mediterranean Sea (with ~7 species per year) (EEA, 2015).

The same conclusion has been reached in a series of works by [START_REF] Zenetos | Annotated list of alien marine species in the Mediterranean with emphasis on worst invasive species[END_REF][START_REF] Zenetos | Additions to the annotated list of marine alien biota in the Mediterranean with special emphasis on Foraminifera and Parasites[END_REF]Zenetos et al. ( , 2010[START_REF] Zenetos | Alien species in the Mediterranean Sea by 2012. A contribution to the application of European Union's Marine strategy framework directive (MSFD). Part 2. Introduction trends and pathways[END_REF], each revising and updating the previous data, respectively. When only the established species are considered, the peak of ~11 species per year accounts for the 1991e2000 period and falls to ~5 species per year after 2010. On the contrary, after 2000 the number of casual records appears to be steady (6 species per yr). It is expected that a number of the newly discovered species in the 2001e2010 period will establish viable populations, leading to an increase of the established AS. However, a negative trend is obvious after 2010. A similar trend was illustrated, but not discussed, by [START_REF] Galil | East is east and West is west? Management of marine bioinvasions in the Mediterranean Sea[END_REF].

Is this negative trend in the established species a positive sign for the biodiversity of the Mediterranean? It could be. On the other hand, the increase in the previous decades could be the result of intensive scientific effort developed following the invasion of the much mediatized "killer alga" Caulerpa taxifolia (Vahl) C. Agardh in the 1990s.

3.4.2.1. Uncertainties. The uncertainties behind datasets on AS reflect the challenge of adequately discovering and reporting new species and mapping their distribution, but also of compiling data at a national/regional level.

Lists of invasive AS (IAS) are essential for preventing, controlling, and reporting on the state of biological invasions. However, these lists suffer from a range of errors, with serious consequences for their use in science, policy, and management [START_REF] Mcgeoch | Uncertainty in invasive alien species listing[END_REF]. No checklist can be accurate by the time it is published. New data come to light and there are continuous changes on species nomenclature, likely date and pathway of introduction, origin, establishment success, and distribution of species. Consequently, every list could be called a 'provisional list'.

The list for Mediterranean AS is still an underestimate, for a number of reasons:

a) The present list does not include unicellular organisms. The diversity of marine microalgae is scarcely known in wide areas of the Mediterranean Sea. This makes it difficult to determine whether a suspected microalgal invader was already present as part of the rare, hidden and un-sampled phytoplankton. Therefore, phytoplankton has not been included in the analysis of the indicator; b) Miscategorising AS species as native is not rare. The Suez Canal is relatively recent, but entries by vessels through Gibraltar have been occurring long before marine biologists started inventorying AS. Many pseudoindigenous species [START_REF] Carlton | Deep invasion ecology and the assembly of communities in historical time[END_REF] occur in the Mediterranean, precisely because many old taxonomic works originated in the Mediterranean, and what was there at the time of these initial inventories was assumed to be native, even if many species were described from ports and areas that were typical of introductions; c) There are many records of new sightings that refer to species which are presently reported as cryptogenic. Phylogenetic studies have revealed that some of them are true aliens. Examples are reported in the section for 'Additional species'. Further studies will shed some light on the rest;

d) The alien status is still pending for many species that are presently categorised as 'questionable' (see Zenetos et al., 2010).

Inventories of the regional Online information systems (e.g. EASIN, MAMIAS) are difficult to keep up-to-date. Comparison of the present work with previous work [START_REF] Zenetos | Alien species in the Mediterranean Sea by 2012. A contribution to the application of European Union's Marine strategy framework directive (MSFD). Part 2. Introduction trends and pathways[END_REF] revealed an increase of approximately 28% in established AS during the previous 5 years [>600 species vs. approximately 500 established species that were reported in [START_REF] Zenetos | Alien species in the Mediterranean Sea by 2012. A contribution to the application of European Union's Marine strategy framework directive (MSFD). Part 2. Introduction trends and pathways[END_REF]].

With regards to the latest published list (Zenetos et al., 2010[START_REF] Zenetos | Alien species in the Mediterranean Sea by 2012. A contribution to the application of European Union's Marine strategy framework directive (MSFD). Part 2. Introduction trends and pathways[END_REF][START_REF] Galil | East is east and West is west? Management of marine bioinvasions in the Mediterranean Sea[END_REF], the difference in the number of established AS per taxonomic groups is demonstrated in Fig. 2. The phyla with the highest increase since 2012 are the Bryozoa (108% increase), followed by Ascidiacea (100%) and Polychaeta (41%). In the groups treated in the CIESM atlases [START_REF] Galil | CIESM Atlas of exotic species in the Mediterranean[END_REF][START_REF] Golani | CIESM e Atlas of Exotic Fishes e List[END_REF][START_REF] Verlaque | Macrophytes[END_REF][START_REF] Zenetos | CIESM Atlas of Exotic Species in the Mediterranean[END_REF]) there seems to be a steady increase of approximately 20%. Considering updates of the [START_REF] Galil | East is east and West is west? Management of marine bioinvasions in the Mediterranean Sea[END_REF] per taxonomic group, in decreasing order, they are major in Ascidiacea (58% of taxa), Polychaeta (57%), Macroalgae (49%), Mollusca (45%), Bryozoa (36%), Crustacea (20%), and these are values that reflect both the taxonomic expertise of the research group and their knowledge of the local biodiversity. It should be noted here that the phylum mollusca might need a further update. Some small sized gastropods species such as Voorwindia tiberiana (Issel, 1869), Iolaea neofelixoides (Nomura, 1936) and Aliculastrum cylindricum (Helbling, 1779) that noted as rare or unknown in [START_REF] Galil | East is east and West is west? Management of marine bioinvasions in the Mediterranean Sea[END_REF], are not discussed here because their true distribution remains unknown. Following [START_REF] Mienis | New data concerning the presence of Indo-Pacific molluscs in the Mediterranean Sea with emphasis on Israel[END_REF] they are provisionally treated as established. Other taxomonic groups such as Cnidaria also need a further update.

Inconsistencies are also observed in comparing the present list with what is archived in EASIN v.5.7, Latest Update: 15/11/2016. This is inevitable due to changes that came to light after November 2016 and not only, but we are confident that EASIN and INVASI-VESNET can play a major role in the future revision/update of the present list, which can currently serve for assessing indicators that are necessary for policy, and management of AS in the Mediterranean.

We fully agree with [START_REF] Galil | East is east and West is west? Management of marine bioinvasions in the Mediterranean Sea[END_REF] that current geographical, taxonomical and impact data gaps can be reduced only by instituting harmonised standards and methodologies for monitoring NIS populations in all countries bordering the Mediterranean Sea. However, their list is another 'provisional list' in which approximately 38% of the species need revision in one of the reported fields (Nomenclature, Year of first record, First recipient country, Population status), while a significant number (>10%) of species are missing. Of the 72 added species, only 12 (4 macroalgae, 1 polychaete, 4 ascidians, 1 bryozoan, 2 cnidarians) were reported for the first time, or confirmed by genetic phylogeographic studies to be introduced, in 2016.

The present list reflects a team effort of regional experts in order to compile the most reliable source of information regarding the current status of invasive species in the Mediterranean. As professionals in this field, all authors of the present paper recognise the fact that even the current list may contain errors due to the dynamic nature of marine invasions, and the addition of new tools enabling accurate taxonomic identifications.

✓

  Symplegma brakenhielmi (Michaelsen, 1904): should be 1975 according to the museum samples at TAU, or 1999 according to Izquierdo-Mun ~oz et al. (2009); ✓ Styela plicata (Lesueur, 1823): Heller 1877 as Styela gyrosa.

Fig. 2 .

 2 Fig. 2. Comparative number of AS in the recent body of literature.

  : recently found in Spain and Italy, and is likely introduced by vessels (Lopez-[START_REF] Lopez-Legentil | Harbor networks as introduction gateways: contrasting patterns of native and introduced ascidians[END_REF]; ✓ Ascidiella aspersa (Müller, 1776): described from Norway A. aspersa is a widespread species that is introduced into many parts of the world. It can be safely assigned an introduced status in the Mediterranean[START_REF] Nishikawa | Molecular and morphological discrimination between an invasive ascidian, Ascidiella aspersa, and its congener A. scabra (Urochordata: Ascidiacea)[END_REF][START_REF] Lopez-Legentil | Harbor networks as introduction gateways: contrasting patterns of native and introduced ascidians[END_REF]. Its vector is most likely vessels, as the species occurs in harbours. The oldest Mediterranean report is that of Delle Chiaje (1828) (as Phallusia mamillaris, according to[START_REF] Traustedt | Die einfachen Ascidien (Ascidiae simplices) des Golfes von Neapel[END_REF] from the Gulf of Naples; ✓ Botrylloides nigrum (Herdman, 1886): reported by Pere 's 1958 from the coast of Israel (see also[START_REF] Sheets | Investigating the widespread introduction of a tropical marine fouling species[END_REF] for a recent report in Israel); ✓ Ciona robusta Hoshino and Tokioka, 1967: the cosmopolitan taxon Ciona intestinalis has been recently shown to comprise several cryptic species, of which the most widespread are the so-called Ciona intestinalis type A and type B[START_REF] Zhan | Invasion genetics of the Ciona intestinalis species complex: from regional endemism to global homogeneity[END_REF].Recent studies[START_REF] Brunetti | Morphological evidence that the molecularly determined Ciona intestinalis type A and type B are different species: Ciona robusta and Ciona intestinalis[END_REF][START_REF] Pennati | Morphological differences between larvae of the Ciona intestinalis species complex: hints for a valid taxonomic definition of distinct species[END_REF] have shown that Ciona intestinalis type A is in fact C. robusta. Genetic data (Bouchemousse et al., 2016) support the introduced status of C. robusta in Europe, based on low genetic diversity as compared with the presumed native area in the NW Pacific. Nowadays, C. robusta can be safely assigned an introduced status in the Mediterranean, being reported there since the 19th Century (Savigny, 1816; as Phallusia intestinalis), most often under the name C. intestinalis; ✓ Clavelina oblonga Herdman, 1880: this is a true introduced species that has been mistakenly described as a new species Clavelina phlegraea Salfi, 1929 in the Mediterranean and is found in Italy, Corsica, and Spain (Ordon ~ez et al., 2016); ✓ Diplosoma listerianum (Milne Edwards, 1841): often cited as cryptogenic, it can be considered as introduced in the Mediterranean. It is a species complex, but the Mediterranean clade is native to the Atlantic shores (Perez-Portela et al., 2013). Della Valle (1877) first reported this species (as Pseudodidemnum listerianum Milne-Edwards, 1841) from the Gulf of Naples; ✓ Microcosmus anchylodeirus Traustedt, 1883: collected in Corsica over a period of at least 12 years

Table 1

 1 Species whose nomenclature has been recently revised.

	Taxon	Species in Galil et al., 2016	Valid name	Source/comment
	Ochrophyta/	Botrytella parva (Takamatsu) H.S.Kim	Botrytella "cf. parva"	It is likely to be B. parva but the identification requires
	Phaeophyceae			a genetic confirmation
	Mollusca	Crassostrea gigas (Thunberg, 1793)	Magallana gigas (Thunberg, 1793)	WoRMS
	Mollusca	Paphia textile (Gmelin, 1791)	Paratapes textilis (Gmelin, 1791)	Huber, 2015
	Mollusca	Gafrarium pectinatum (Linnaeus, 1758)	Gafrarium savignyi (Jonas, 1846)	Huber, 2010
	Mollusca	Tellina valtonis Hanley, 1844	Nitidotellina unifasciata (G.B. Sowerby II, 1867) Huber, 2015
	Mollusca	Acanthopleura gemmata (Blainville, 1825)	Acanthopleura sp.	Crocetta et al., 2014
	Mollusca	Circenita callipyga (Born, 1778)	Circenita varia (Forsskål in Niebuhr 1775)	Huber, 2015
	Mollusca	Mytilopsis sallei (Recluz, 1849)	Mytilopsis adamsi J. P. E. Morrison, 1946	Huber, 2015
	Mollusca	Timoclea marica (Linnaeus, 1758)	Timoclea roemeriana (Issel, 1869)	Huber, 2015
	Mollusca	Elysia grandifolia Kelaart, 1858	Elysia ornata (Swainson, 1840)	Jensen, 2015
	Mollusca	Amphioctopus aegina (Gray, 1849)	Amphioctopus cf. aegina (Gray, 1849)	Crocetta et al., 2014
	Polychaeta	Pherusa saldanha Day, 1961	Stylarioides grubei Salazar-Vallejo, 2011	Misidentification Zenetos et al., 2012
	Crustacea	Macrophthalmus (Macrophthalmus) graeffei A.	Macrophthalmus indicus Davie, 2012	Davie, 2012
		Milne-Edwards, 1873
	Crustacea	Gonioinfradens paucidentatus (A. Milne-	Charybdis (Gonioinfradens) paucidentatus (A.	WoRMS
		Edwards, 1861)	Milne-Edwards, 1861)
	Bryozoa	Celleporaria brunnea (Hincks, 1884)	Celleporaria sp. aff. brunnea	Harmelin, 2014
	Bryozoa	Electra tenella (Hincks, 1880)	Arbopercula tenella (Hincks, 1880)	Rosso and Di Martino, 2016
	Fish	Apogon queketti Gilchrist, 1903	Jaydia queketti (Gilchrist, 1903)	Golani et al., 2016 (CIESM)
	Fish	Apogon smithi (Kotthaus, 1970)	Jaydia smithi Kotthaus, 1970	Golani et al., 2016 (CIESM)
	Fish	Atherinomorus lacunosus (Forster, 1801)	Atherinomorus forskali (Rüppell, 1838)	Golani et al., 2016 (CIESM)
	Fish	Lagocephalus spadiceus (Richardson, 1845)	Lagocephalus guentheri Miranda Ribeiro, 1915 Misidentification: Matsuura et al., 2011
	Fish	Pempheris vanicolensis Cuvier, 1831	Pempheris rhomboidea Kossmann and Ra €uber, Misidentification: Azzurro et al., 2015
			1877
	Fish	Oxyurichthys papuensis (Valenciennes, 1837)	Oxyurichthys petersi (Klunzinger, 1871)	Golani et al., 2016 (CIESM)
	Fish	Bregmaceros atlanticus Goode and Bean, 1886 Bregmaceros nectabanus Whitley 1941	Misidentification: Harold and Golani, 2016
	Cnidaria/	Sertularia thecocarpa (Jarvis, 1922)	Sertularia tongensis (Stechow, 1919)	WoRMS
	Hydrozoa
	Cnidaria/	Pelagia benovici Piraino, Aglieri, Scorrano &	Mawia benovici (Avian, Ramsak, Tirelli,	Avian et al., 2016
	Scyphozoa	Boero, 2014	D'Ambra and Malej, 2016)
	Ctenophora	Beroe ovata Bruguie 're, 1789	Beroe ovata sensu Mayer	WoRMS

Table 2

 2 Discrepancies in first collection/observation date and country. The revised data are in bold. The non-established species are in gray-colour shading.

	Macroalgae	Acrothamnion preissii (Sonder) E.M.Wollaston	1966e69	Italy
	macroalgae	Asparagopsis taxiformis [lineage 2] (Delile) Trevisan de Saint-Leon	1993	Spain
	macroalgae	Bonnemaisonia hamifera Hariot	1909	Tunisia
	macroalgae	Caulerpa cylindracea Sonder	1985	Tunisia
	macroalgae	Chondria curvilineata F.S.Collins & Hervey	1980	Greece
	macroalgae	Cladophora patentiramea (Montagne) Kützing	1991	Cyprus
	macroalgae	Codium fragile subsp. fragile (Suringar) Hariot	1946	France
	macroalgae	Codium taylorii P.C.Silva	1955	Israel
	macroalgae	Colaconema codicola (Børgesen) H.Stegenga, J.J.Bolton & R.J.Anderson	1952	France
	macroalgae	Derbesia rhizophora Yamada	1984	France
	macroalgae	Derbesia boergesenii (Iyengar & Ramanathan) Mayhoub	1972	Syria
	macroalgae	Fucus spiralis Linnaeus	1987	France
	macroalgae	Galaxaura rugosa (J.Ellis & Solander) J.V.Lamouroux	1990	Syria
	macroalgae	Hypnea anastomosans Papenfuss, Lipkin & P.C.Silva	1972	Israel
	macroalgae	Hypnea cornuta (Kützing) J.Agardh	1894	Greece
	macroalgae	Hypnea valentiae (Turner) Montagne	1996	France
	macroalgae	Laurencia okamurae Yamada	1984	France
	macroalgae	Leathesia marina (Lyngbye) Decaisne	1905	France
	macroalgae	Lomentaria hakodatensis Yendo	1978	France
	macroalgae	Plocamium secundatum (Kützing) Kützing	1976	Spain
	macroalgae	Polysiphonia morrowii Harvey	1997	France
	macroalgae	Pterosiphonia tanakae S.Uwai & M.Masuda	1993	France
	macroalgae	Sarconema filiforme (Sonder) Kylin	1944	Israel
	macroalgae	Sarconema scinaioides Børgesen	1945	Israel
	macroalgae	Scytosiphon dotyi M.J.Wynne	1960e77	Italy
	macroalgae	Spatoglossum variabile Figari & De Notaris	1944	Egypt
	macroalgae	Sphaerotrichia firma (E.S.Gepp) A.D.Zinova	1970	Turkey
	macroalgae	Ulva ohnoi M.Hiraoka & S.Shimada	2011	Italy
	macroalgae	Uronema marinum Womersley	1989	France
	Mollusca	Acteocina mucronata (Philippi, 1849)	1986	Turkey/Lebanon
	Mollusca	Amathina tricarinata (Linnaeus, 1767)	2000	Turkey/Lebanon
	Mollusca	Anadara kagoshimensis (Tokunaga, 1906)	1965e69	Italy
	Mollusca	Arcuatula perfragilis (Dunker, 1857)	1934	Egypt
	Mollusca	Bursatella leachii Blainville, 1817	1940	Israel
	Mollusca	Cerithidium perparvulum (Watson, 1886)	1992	Turkey/Cyprus
	Mollusca	Chama asperella Lamarck, 1819	1993	Turkey
	Mollusca	Conus fumigatus Hwass in Brugui e ' re, 1792	1974e 76	Libya
	Mollusca	Magallana gigas (Thunberg, 1793)	1905	Italy
	Mollusca	Cycloscala hyalina (G. B. Sowerby II, 1844)	1992	Cyprus
	Mollusca	Diodora ruppellii (G. B. Sowerby I, 1835)	1939	Israel
	Mollusca	Favorinus ghanensis Edmunds, 1968	2003	Tunisia
	Mollusca	Finella pupoides A. Adams, 1860	1958	Israel/Turkey
	Mollusca	Fulvia australis (G. B. Sowerby II, 1834)	1937	Israel
	Mollusca	Fulvia fragilis (Forsskål in Niebuhr, 1775)	1934	Egypt
	Mollusca	Godiva quadricolor (Barnard, 1927)	1985e86	Italy
	Mollusca	Goniobranchus annulatus (Eliot, 1904)	2000	Lebanon
	Mollusca	Mactra olorina Philippi, 1846	1883	Egypt
	Mollusca	Marginella glabella (Linnaeus, 1758)	2009	Spain
	Mollusca	Modiolus auriculatus (Krauss, 1848)	1934	Israel
	Mollusca	Monotygma fulva (A. Adams, 1853)	2000	Turkey
	Mollusca	Mya arenaria Linnaeus, 1758	1984	Greece
	Mollusca	Notocochlis gualteriana (R ecluz, 1844)	1961	Israel
	Mollusca	Oscilla galilae Bogi, Karhan and Yokes ¸ , 2012	1992e95	Turkey
	Mollusca	Mytilopsis adamsi J. P. E. Morrison, 1946	2002	Egypt
	Mollusca	Paratapes textile (J. F. Gmelin 1791)	1948	Israel
	Mollusca	Petricola fabagella Lamarck, 1818	1905	Egypt
	Mollusca	Petricolaria pholadiformis (Lamarck, 1818)	1985	Greece
	Mollusca	Pseudochama corbierei (Jonas, 1846)	1938	Greece
	Mollusca	Pseudominolia nedyma (Melvill, 1897)	1959	Israel
	Mollusca	Pseudorhaphitoma iodolabiata (Hornung and Mermod, 1929)	2010	Israel/Turkey
	Mollusca	Sabia conica (Schumacher, 1817)	1954	Israel
	Mollusca	Septifer cumingii Recluz, 1849	1999	Turkey
	Mollusca	Siphonaria crenata Blainville, 1827	1934	Egypt
	Mollusca	Spondylus spinosus Schreibers, 1793	1987e88	Israel
	Mollusca	Syphonota geographica (A. Adams and Reeve, 1850)	1999	Italy
	Mollusca	Syrnola lendix (A. Adams, 1863)	1988e89	Turkey
	Mollusca	Tremoctopus gracilis (Eydoux and Souleyet, 1852)	1937	Croatia
	Mollusca	Trochus erithreus Brocchi, 1821	1968	Israel
	Polychaeta	Ceratonereis mirabilis Kinberg, 1865	1969	Egypt
	Polychaeta	Eusyllis kupfferi Langerhans, 1879	1998	Cyprus
	Polychaeta	Glycinde bonhourei Gravier, 1904	1937	Israel
	Polychaeta	Hesionura serrata (Hartmann-Schro €der, 1960)	1978	Spain
	Polychaeta	Leodice antennata Savigny in Lamarck, 1818	1975e81	Italy
	Polychaeta	Leonnates persicus Wesenberg-Lund, 1949	1968	Egypt
	Polychaeta	Linopherus canariensis Langerhans, 1881	1965	Lebanon

Fig. 1. Number of new AS per year at 10-yr intervals.

Acknowledgements

We would like to thank Dr. F. Monniot for providing us with first record dates and details for ascidians in her collection and Dr. Serhat Albayrak and J. Van Aartsen for first records for mollusca in his collection.The following colleagues provided details and valuable comments on the species: Dr. Geoff. A. Boxshall on the native status of Nothobomolochus fradei, Dr. Ernesto Azzuro on the status of Abudefduf spp, Dr. Maria Jesús Uriz concerning introduced sponge species and, Dr Luisa Borges for details on the occurrence of Teredothyra dominicensis.

This work was initiated during the DEVOTES (DEVelopment Of innovative Tools for understanding marine biodiversity and assessing good Environmental Status) project, funded by the European Union under the 7th Framework Program (grant agreement no. 30839). The work of FC and AZ was partly funded by The East and South European Network for Invasive Alien Species -a tool to support the management of alien species in Bulgaria (ESENIAS-TOOLS) (European Economic Area funded -Contract No. D-33-51/ 30.06.2015). GS and FC were supported by STSM contracts in the framework of Alien Challenge COST Action (TD1209) funded by the European Commission.

Appendix A. Supplementary data

Supplementary data related to this article can be found at http:// dx.doi.org/10.1016/j.ecss.2017.03.031.