Brice Libert

Tonfack

Emmanuel Youmbi

Akoa Amougou

Anne Bernadac

Effect of Organic/Inorganic-Cation Balanced Fertilizers on Yield and Temporal Nutrient Allocation of Tomato Fruits under Andosol Soil Conditions in Sub-Saharan Africa

Keywords: Solanum lycopersicum, yield, nutrient accumulation, andosol, organic manure, cation balance

The impact of organic fertilizers on plant behaviour under andosol conditions is not well understood. A field experiment was conducted on a silty and slightly acidic andosol, very poor in phosphorous (3 mg kg - 1 of soil) and potassium (0.13 meq/100g of soil), with an imbalance Ca:Mg:K ratio of 74.0:25.0:0.7. The effect of integrated mineral fertilization (using N, P, K, Ca and Mg), dry poultry manure (organic), the association of the two (organo-mineral) fertilizers and non-integrated mineral fertilization has been studied on yield and nutrient allocation of two varieties of tomato (Solanum lycopersicum L. var. Roma VF and Tima). Yields of red tomatoes from organo-mineral fertilized plants (39.27-34.38 t ha -1 ) were three times higher than the yields from plants fertilized with non-integrated minerals (12.97-11.59 t ha -1 ). Allocation of macronutrients (P, K and Ca) was high in fruits from plants fertilized with organic, organomineral and integrated mineral fertilizers. Fruit calcium increased mostly at the fruit ripening stage, while phosphorus and potassium accumulated mainly before the fruit ripening stage. This trend was by far more pronounced under organo-mineral and organic fertilization. Overall, the kinetics of nutrient allocation indicate a clear positive impact of poultry manure and organo-mineral fertilizers in improving tomato production on andosol.

Introduction

A correct soil nutrient balance is essential for healthy growth and high crop productivity [START_REF] Tonfack | Impact of organic and inorganic fertilizers on tomato vigour, yield and fruit composition under tropical andosol soil conditions[END_REF]. Fruit production is directly associated to the allocation of nutrients in sink organs. Tomatoes are strong sink organs but their production remains challenging under tropical soils developed from volcanic materials. The western region of Cameroon is partially covered by volcanic materials. Its geological substratum is constituted of a thick layer of pyroclastites on which has developed a soil classified according to the world referential basis of resources in soil, as slender andosols (FAO-ISRIC, 2006) also known as andisols or volcanic ash soils. These soils are considered to have an average to high agricultural potential [START_REF] Bationo | African soils: Their productivity and profitability of fertilizer use[END_REF] and are widely cropped [START_REF] Sansoulet | Adsorption and transport of nitrate and potassium in an andosol under banana (Guadeloupe, French West Indies)[END_REF]. Despite the fact that yields remain low (about 18 tonnes of tomatoes per ha), tomato production has become a current practice as it contributes to poverty alleviation of smallholders' households by enhancing their income (World Vegetable Centre, 2007). Farmers are suffering from declining soil fertility and are complaining about weak responses of their soil fertility management. Investigations have shown that farmers mostly rely on a single option of conventional nutrient replenishment, without taking into account the soil mineral balance. This agricultural system enhances negative soil nutrient balance and weak response of non-integrated mineral fertilization [START_REF] Tonfack | Impact of organic and inorganic fertilizers on tomato vigour, yield and fruit composition under tropical andosol soil conditions[END_REF].

Actually, the challenge is to reach the agroclimatically potential yield of crops, using the best available technologies and farming practices to avoid abiotic stresses [START_REF] Tittonell | The diversity of rural livelihoods and their influence on soil fertility in agricultural systems of East Africa-A typology of smallholder farms[END_REF][START_REF] You | Exploiting strategic priorities for regional agricultural R&D investments in East and Central Africa[END_REF] and enhance nutrient accumulation in sink organs. Many scientists have pointed out the importance of organic fertilizers in highly degraded soils (Bationo and Buerker, 2001;[START_REF] Yamoah | Rotation effect on sorghum response to nitrogen fertilizer under different rainfall and temperature environments[END_REF] and concluded that their judicious use in agriculture, in condition of scarcity of existing resources is critical for maintaining or increasing production [START_REF] Keiichi | Evaluation of the utilization of heated sewage sludge for peri-urban horticulture production in the Sahel of West Africa[END_REF]. Poultry manure is a highly rich organic fertilizer that is easily available, cheaper, easier to apply and sustainable that could be a good strategy to sustain tomato production under tropical andosol soil conditions.

The aim of the present study was to investigate the pattern of mineral and carbohydrate allocation in tomatoes under an alternative method of organic fertilization as well as integrated inorganic fertilization management on an andosol soil condition.

Material and Methods

Culture Conditions and Experimental Design

An open field experiment was carried out at the research station of the Institute of Agricultural Research for Development (IRAD), Foumbot (10° 36'-10° 37' E, 5° 29'-5° 30' N, altitude 1100 m) Cameroon.

The seeds of two tomato varieties (Solanum lycopersicum L., cv. Roma V and Tima adapted to tropical conditions, with a medium harvest homogeneity and potential yield of 50-60 t ha -1 ) produced by Technisem (Savigny -sur -Orge, France), were sown on September 21 st . Forty-daysold seedlings were transplanted in three rows (four plants per row) at 1.0 m distance between rows and 0.5 m distance within rows, into the 6.0 m 2 plots. The experimental design was a split-plot with the varieties as main plots and five different fertilizers as subplots. Each treatment had four replicates. Climatic conditions prevailing during the experimental period were registered (Table 1). In the absence of rain, plants received water by capillary rise of water from irrigation. Plants were treated twice against insects and fungi with endosulfan and mancozeb respectively.

Soil and Manure Analysis

In order to elaborate fertilization plans, soil and manure were analyzed. The andosol soil sample was collected at 15 cm depth in five different places all over the 600 m 2 experimental garden, mixed and analyzed for texture and chemical characteristics. Soils were air-dried and ground to pass through a 2 mm sieve. Soil pH in water was determined in a 1:2.5 (w/v) soil:water suspension. Organic C was determined by chromic acid digestion and spectrophotometric analysis [START_REF] Heanes | Determination of organic C in soils by an improved chromic acid digestion and spectrophotometric procedure[END_REF]. Total N was determined from a wet acid digest [START_REF] Buondonno | Comparing tests for soil fertility. The hydrogen peroxide/sulfuric acid treatment as an alternative to the copper/selenium catalyzed digestion process for routine determination of soil nitrogen-Kjeldahl[END_REF] by colorimetric analysis [START_REF] Anderson | Tropical soil biology and fertility: A handbook of methods[END_REF]. Exchangeable Ca, Mg, K and Na were extracted using the Mehlich procedure and determined by atomic absorption spectrophotometry. Available P was extracted by the Bray-1 procedure and analyzed using the molybdate blue procedure described by [START_REF] Murphy | A modified single solution method for determination of phosphate in natural waters[END_REF]. Seven months old poultry manure was collected from a local farmer and analyzed for chemical characteristics as follows: cations (Ca, Mg and K) were extracted by dry ashing in a muffle furnace at 500°C, diluted using aqua regia (acid mix of HCl/HNO 3 ) and analyzed using an atomic absorption spectrophotometer. Phosphorus was extracted by dry ashing and analyzed by colorimetry [START_REF] Murphy | A modified single solution method for determination of phosphate in natural waters[END_REF]. Data are reported as a percentage of dry matter. Total N was determined from a wet acid digest [START_REF] Buondonno | Comparing tests for soil fertility. The hydrogen peroxide/sulfuric acid treatment as an alternative to the copper/selenium catalyzed digestion process for routine determination of soil nitrogen-Kjeldahl[END_REF] by colorimetric analysis [START_REF] Anderson | Tropical soil biology and fertility: A handbook of methods[END_REF].The soil was loamy sand and slightly acidic. Soil apparent density was also very low, revealing a very porous soil and high organic matter content. Organic matter and nitrogen content were high, with a good potential for nitrogen mineralization. On the other hand, available phosphorous content was particularly low (3 mg P per kg of soil) and the soil was also very poor in potassium (0.13 meq/100g of soil). Exchangeable cation values revealed that the soil was profoundly unbalanced with excess of magnesium and insufficient levels of potassium [(Ca:Mg:K) = (74.0:25.0:0.7)]. Poultry manure was slightly alkaline and rich in macronutrients with very high content of cations. The [C:N] ratio was 9.3, revealing high nitrogen content and a good capacity for mineralization (Table 2).

Fertilization Treatments

Five different treatments were applied:

-F0: unfertilized soil;

-F1: integrated mineral fertilizer (IMF) providing 94 g N, 4 g P 2 O 5 , 20.1 g K 2 O, 12.5 g CaO and 1.6 g MgO per plant. These different mineral doses were calculated with respect to tomato plants' demand for a maximal fruit output of 50-60 t ha -1 in tropical region [START_REF] Caburet | Les légumes : la tomate[END_REF]. This contributed to a soil final Ca:Mg:K ratio of 75.2:18.0:5.8. This fertilizer was fractionated in three applications: 50% N, 80% K 2 O, 100% P 2 O 5 , 100% MgO and 84% CaO were applied 1 week before transplanting; then 25% N, 10% K 2 O and 8% CaO were applied 2 and 4 weeks after transplanting;

-F2: poultry manure as organic fertilizer (OF) was applied 2 weeks before transplanting at 2 kg plant -1 and completely mixed with soil, contributing to a Ca:Mg:K ratio of 68:24:7; -F3: organo-mineral fertilizer (OMF) consisted of the F1 and F2 on the same plots;

-F4: non-integrated mineral fertilizer (NMF) providing 6 g N, 3 g P 2 O 5 and 3 g K 2 O per plant was applied 1 week after transplanting and completed 3 weeks later with 2.5 g N plant -1 as practiced by local farmers. The values are means of five soil samples randomly taken from the experimental field and three measurements of seven months old poultry manure.

Fruit Sampling, Sugar and Mineral Analysis

Sampling took place on a daily basis and was performed randomly from the third trusses of four plants. Fruits were tagged at setting (diameter 2 mm). Tagged fruits were collected at immature green (IMG), mature green (MG) and red ripe (RR). These stages corresponded to 45-50, 60-65 and 85-90 days post transplanting. Fruits were transported to the laboratory in refrigerated containers. At each developmental stage, four fruits were collected on the third truss of four different plants of the same plot, with three replications. The twelve fruits were used to determine fresh weight and then, nine of them were oven-dried at 65°C until constant weight was aquired. The dry samples were used to determine dry matter and three of them were homogenized and used for the determination of macronutrient (P, K and Ca) contents. Macronutrients were measured after dry ashing at 550°C by flame emission (K) or atomic absorption (Ca) spectrophotometry [START_REF] Hanlon | Determination of Potassium, Calcium, and Magnesium in Plants by Atomic Absorption Techniques[END_REF]. P was determined using vanado molybdate colorimetry. Three fresh fruits were freezed at -20°C until used for sugar analysis. Fruits were ground, and centrifugated for 10 mn at 3500 rpm for juice extraction. Glucose and fructose concentrations were analysed using the EnzyPlus Sucrose/D-glucose/D-fructose enzymatic analysis method [START_REF] Bergmeyer | Methods of enzymatic analysis[END_REF].

Fruits were collected at turning to red ripe stages for yield determination.

Statistical Analysis of the Data

Data were subjected to one-way and two-way ANOVA to determine significant differences between fertilization treatments and varieties, and interaction between fertilization and variety. The Student Newman-Keuls test at the 0.05% significance level was used to calculate Least Significant Differences (LSD).

Results

Temporal Fruit Mean Weight and Dry Matter Content

Generally, fruit mean weight increased between IMG and RR stages in all plots (Table 3). From the IMG to the MG stage, the fruit mean weight multiplied 2 to 3 times while between the MG and the RR stages, fruit mean weights increased slightly. Greater differences between fertilization treatments were found at IMG (p< 0.0001) and MG stages (p<0.0001). At MG, greater fruit mean weights were observed on plants (var. 'Roma VF' and 'Tima') fertilized with OF (51.43 and 70.45 g), OMF (50.84 and 64.19 g) and IMF (35.93 and 53.01 g). At RR stage, there was a significant difference between fertilization treatments for fruit mean weight, and the higher mean weights were observed on variety "Tima" (60.67-92.59 g).

Fruit dry matter was higher at IMG stage (5.66-7.46%) and decreased at MG (3.99-5.44%) and RR stages (3.36-5.03%) (Table 3). At IMG stage, fruit dry matter was significantly different (p= 0.049) between the fertilizers, while at MG stage, the difference was observed for interaction varietyfertilizer (p= 0.039). At RR stage, there was no difference between treatments.

Yield

In terms of the number of fruits per plant and total yield results, the differences were highly significant between fertilizers (p<0.0001) (Figure 1). The number of tomatoes 'Roma VF' per plant was also highly superior to that of 'Tima'. As compared to NMF, OF and OMF highly improved the number of fruits per plant and the yield of the two varieties. The rates of increase of the number of fruit per plant in organic plots were 159% and 127% when compared to NMF, for Roma VF and Tima respectively. In OMF plots, the rates of increase of the number of fruits per plant were 176% and 191% compared to NMF for Roma VF and Tima respectively. The rate of increase of the yield by IMF was 112% and 39% respectively for the varieties Roma VF and Tima, compared to NMF. The yields obtained with OF (34.6-33.5 t ha - 1 ) were not statistically different from that obtained with OMF (39.2-34.4 t ha -1 ). Compared to NMF, OF induced increase yield rates of 168% and 189% in tomatoes Roma VF that of Tima respectively.

Temporal Accumulation of Nutrients in Fruit

Phosphorus accumulation increased during tomato fruits' development, but the increase depended on variety and fertilizer (Figure 2). At all stages of tomato fruits' development, P was more accumulated in tomato fruits cultivated with OF and OMF. During the early phase of tomato development, plants fertilized with OF and OMF accumulated significantly more phosphorous than other fertilizers, reaching 300-342 mg.plant -1 for Roma VF and 227-254 mg.plant -1 for Tima at IMG stage. At RR stage, the total quantity of P accumulated in Tima fruits increased more than three times when plants were fertilized with OF, reaching a value of 737.87 mg.plant -1 .

The time course of K accumulation in tomato fruits depended on varieties and fertilization (Figure 2). Plants fertilized with OF and OMF each accumulated more K than plants submitted to other fertilizers. K accumulation in the fruits of Roma VF was higher during fruit maturation. At MG stage, tomato plants fertilized with OF and OMF accumulated 4963 and 5168 mg of K respectively in their fruits. At RR stage, the quantity of K accumulated (4776 and 6133 mg plant -1 ) was not different from that accumulated at MG stage. In var. Tima, K accumulation increased continuously throughout the period of fruit development. In plots fertilized with IMF, OF and OMF, plants respectively accumulated 1771, 4232 and 4608 mg K plant -1 . At RR stage, these values increased to 2682, 6259 and 5274 mg plant -1 respectively. For all fertilization treatments, Ca accumulation in tomato fruits was higher during the last 25 days of fruit development (Figure 2). Tomato plants accumulated more Ca when fertilized with OF and OMF. At MG stage, plants fertilized with OF and OMF accumulated 179 mg of Ca plant -1 for Roma VF and 185 mg of Ca plant -1 for Tima. At RR stage, Ca accumulated by plants fertilized with OF and OMF increased to 248 mg of Ca plant -1 for Roma FV and 392 mg of Ca plant -1 for Tima. The kinetic of Ca accumulation showed a rise during the last 25 days of fruit development. IMG: immature green; MG: mature green; RR: red ripe.

Roma VF

Temporal Fruit Sugar Contents

The concentrations of glucose and fructose in tomatoes increased significantly during fruit development in all varieties (Figure 3). Glucose and fructose concentrations increased most between the MG and the RR stages. Fertilizations induced a slight increase of fruit glucose and fructose concentrations at all stages of fruits development and the tomatoes produced with OMF had the highest glucose and fructose concentrations at RR stage. However, tomatoes produced with OF had their sugar content slightly inferior to that produced with OMF, but superior to that produced with IMF. Glucose concentration in fresh fruits harvested on plots fertilized with OMF increased from 14.03 to 16.33 mg g -1 FW for Roma V and from 13.71 to 15.41 mg g -1 FW for Tima while fructose concentrations moved from 12.73 to 17.69 mg g -1 FW for Roma VF and from 11.85 to 17.07 mg g -1 FW for Tima.

Discussion

In this experiment, the kinetics of tomato fruits nutrient accumulation varied as a function of variety and fertilization under an andosol soil condition in sub-Saharan Africa. At early stages of fruit development, there were highly significant effects of fertilization and variety on fruit mean weight and dry matter but there were no significant effect of fertilizer on fruit mean weight and fruit dry matter content at RR stage. Thus fertilization affected the speed of fruit loading, but not the final fruit mean weight and dry matter content. Tomato fruit mean weight increased from the tender stage to the mature stage and during the same time, fruit dry matter content decreased. Tomato fruit possesses strong sink capacities and its growth is largely determined by the import of water, nutrients and assimilates brought from other parts of the plant ( [START_REF] Van-Leperen | Distribution of xylem hydraulic resistance in fruiting truss of tomato influenced by water stress[END_REF]. Tomato yield was significantly different between fertilization treatments but not between varieties. These differences in yield may not be induced by fruit mean weight or the quantity of nutrients accumulated in each fruit at the red ripe stage, but by the total quantity of nutrients accumulated in the fruits by the whole plant. This observation is true as the number of fruits carried per plant was highly different between fertilizers and between varieties. The number of fruits per plant was significantly higher on variety Roma VF, but the mean weight of variety Tima was significantly higher at RR stage, hence a similar yield between the 2 varieties. These varieties are equally adapted to andosol soil conditions as attested by the seed producer. OF and OMF gave significantly higher yields, indicating that organic fertilizer offered a better balanced nutrient composition in the soil.

The correct balance of mineral nutrients is essential for healthy growth and high productivity in tomato plants [START_REF] Malone | Mobilization of calcium in glasshouse tomato plants by localized scorching[END_REF][START_REF] Tonfack | Impact of organic and inorganic fertilizers on tomato vigour, yield and fruit composition under tropical andosol soil conditions[END_REF]. Under andosol soil condition in sub-Saharan Africa, OF and OMF might improve nutrient availability to tomato plants. It is known that OF improves mineralization-immobilization patterns, is an energy source for microbial activities, is a precursor to soil organic matter and reduces phosphorus sorption of the soil [START_REF] Palm | Combined use of organic and inorganic nutrient sources for soil fertility maintenance and replenishment[END_REF]. These conditions favour tomato plant vigour and setting and may influence the speed of fruit nutrient accumulation.

In this experiment, P, K and Ca accumulation pattern varied, depending on the fertilization treatment and the tomato variety. Minerals were less accumulated by tomato plants fertilized with NMF and control, while IMF, OF and OMF remarkably enhanced mineral allocation. The pattern of Ca allocation was similar to that mentioned by [START_REF] Tonfack | Impact of organic and inorganic fertilizers on tomato vigour, yield and fruit composition under tropical andosol soil conditions[END_REF] who worked with other varieties, and found that the rate of Ca absorption was maximal during the phase of rapid cell expansion, and Ca accumulation does not increase as much as the rate of fruit growth during the same phase. Thus, fruit Ca accumulation can reach its maximal value after the phase of rapid cell expansion.

Mineral accumulation in tomato fruits from plants fertilized with IMF was lower than that of plants fertilized with poultry manure. This may be due to the multiple roles of organic manures. In fact, organic manure proves to be very satisfactory for the nutritional needs of the horticultural crops in sub-Saharan Africa [START_REF] Keiichi | Evaluation of the utilization of heated sewage sludge for peri-urban horticulture production in the Sahel of West Africa[END_REF]. Under tropical andosol soil condition, poultry manure applied one month before transplanting not only allows keeping soil fertility, but also improves soil structure and availability of mineral elements. Organic phosphorus in poultry manure strongly integrates the pool of soil steady organic matter and organic colloids prevent soluble phosphates from linking with soluble Fe and Al in acidic soils. Moreover, fulvic acids from poultry manure might have significant carboxyl and hydroxyl phenolic contents that form cation complexes to greater level and therefore increase phosphorus availability to plant. [START_REF] Ho | A cellular hypothesis for the induction of blossom-end rot in tomato fruit[END_REF] said that organic fertilizer optimizes Ca uptake by optimizing the soil mineral composition, avoiding excessive K and Mg concentrations, maintains adequate Ca concentration and prevents extreme root temperature and drying in roots environment.

It can be stated that in condition of excessive Mg and poor K and P content, poultry manure restores exchangeable cation balance.

K is transported in the plant readily in the phloem while Ca appears to be virtually immobile in the phloem [START_REF] Malone | Mobilization of calcium in glasshouse tomato plants by localized scorching[END_REF] and is transported through the plant almost entirely via the xylem [START_REF] Adams | Effects of environment on the uptake and distribution of calcium in tomato and on the incidence of blossom-end rot[END_REF]. It is also thought that in tomato plant, water import via the xylem almost completely ceases approximately 25 days after anthesis [START_REF] Ho | An analysis of the accumulation of water and dry matter in tomato fruit[END_REF]. The restriction of water transport via the xylem may almost limit the import of calcium to the fruit ( [START_REF] Van-Leperen | Distribution of xylem hydraulic resistance in fruiting truss of tomato influenced by water stress[END_REF]. Results in the present experiment indicate no limiting calcium accumulation in the later phase of fruit maturation. The absence of limiting calcium accumulation may be explained by the relatively high temperature (>30°C) in the experimental area. Temperature has a considerable effect on tomato fruit maturation [START_REF] Darawsheh | Season-dependent fruit loading: Effect on dry mass, water and nitrogen allocation in tomato plants[END_REF] since high temperature increases absorption of water and essential element ions, probably due to a reduced resistance in water translocation.

Carbohydrate content greatly increased at the later stage of fruit development. This may be another effect of high temperature since in conditions of high temperature, plant respiration increases as a consequence of the temperature sensitivity of the enzymatically catalyzed reactions involved in respiration and of the increased ATP requirements. This induces an increase in metabolic reactions such as sucrose decomposition by fructokinase and an increase in energy demands by the plant to support the increased rate of biosynthesis, transport and protein turnover that occur at high temperature.

Conclusion

Accumulation of nutrients in tomatoes is affected by organic and inorganic fertilizers under andosol of sub-Saharan Africa. For integrated mineral fertilizer, cation ratio should be balanced and by fractionating and applying all essential elements during the entire period of plant growth and fruit development, one could improve the fruit mineral accumulation in tropical andosol. Since poultry manure is cheaper, widely available and easier to apply, the results of the present study clearly indicate that if OF in general and poultry manure in particular is widely adopted by local farmers and applied in andosol, tomato productions and fruit quality will be boosted.

Figure 1 :

 1 Figure 1: Number of fruits per plant and total yield of tomatoes collected at mature green or turning. Grey bars represent tomato yields while white triangles represent the number of fruits per plant. *, †: Values were significantly different to Non-integrated mineral value at 0.05 and 0.01 probability levels. Bars bearing different letters are significantly different by LSD (Student Newman-Keuls test at p<0.05).

Figure 2 :

 2 Figure 2: Macronutrient accumulation in tomatoes at different stages of fruit maturity. IMG: immature green; MG: mature green; RR: red ripe. *, †, ‡: At each stage of fruit maturity, value of the treatment was significantly different to Non-integrated mineral value at 0.05, 0.01 and 0.001 probability levels.

Figure 3 :

 3 Figure 3: Time-course variation of glucose and fructose concentrations in tomatoes.

Table 1

 1 Climatic conditions prevailing on the experimental field, during the experiment

	Month Total	Temperature (°C)	Total
		precipita-			sunlight
		tions	Minimal Maximal	(hours)
		(mm)
	Sep	292.5	15.0	27.0	119.4
	Oct	316.5	15.0	27.2	98.6
	Nov	6.7	14.1	30.9	121.0
	Dec	0.0	12.3	30.1	195.0
	Jan	46.0	14.8	29.6	188.0
	2006

Source: Meteorology section, Institute of Agronomic Research and Development of

Foumbot-Cameroon, 2006.

Table 2

 2 Properties of poultry manure and andosol

	ANDOSOL			POULTRY MANURE
	Parameters	Units	Values	Parameters	Units	Values
	Density	Kg/dm 3	0.74	pH-H 2 O	units	8.77
	Sand	%	50.68	Organic C	%	22.17
	Clay	%	20.32	Total N	%	2.379
	Loam	%	29	C/N	%	9.319
	pH-H 2 O Organic matter	units %	6.24 9.04	Ca 2+ K +	% %	1.26 3.223
	Total C	%	5.02	Na +	%	0.365
	N	%	0.4	Mg 2+	%	0.527
	C/N	/	12.66	P	%	2.532
	Ca 2+	meq/100g	14.66	DW	%	74.89
	K +	meq/100g	0.13
	Na +	meq/100g	1.51
	Mg 2+	meq/100g	4.93
	Bray P	ppm	3.05
	SEB	(meq/100g)	21.23

Table 3

 3 Fruit mean weight and dry matter content of tomatoes at the different developmental stages

	Treatments	Fruit mean weight (g)		Dry matter (%)
	Variety	Fertilizer	IMG	MG	RR	IMG	MG	RR
	Roma VF control	4.83 a	19.69 a	43.21 a	5.66 a	5.44	3.71
		Integrated mineral 18.16 bc	35.93 b	50.81 a	6.08 ab	4.11	4.44
		Organic	17.83 bc	51.43 c	51.96 a	6.73 ab	4.25	3.91
		Organic-mineral	19.08 c	50.84 c	51.38 a	6.14 ab	4.80	4.34
		Non-integrated	13.40 bc	37.90 b	47.04 a	7.46 b	4.71	5.03
		mineral
	Tima	control	10.75 b	41.23 b	63.37 b	6.21 ab	4.55	4.38
		Integrated mineral 16.09 bc	53.01 c	68.27 b	6.86 ab	5.38	3.36
		Organic	27.16 d	70.45 e	92.59 c	6.88 ab	3.99	4.21
		Organic-mineral	23.10 d	64.19 de	60.67 b	6.68 ab	4.14	4.10
		Non-integrated	21.05 cd	58.25	84.97 c	6.46 ab	4.01	4.32
		mineral
		Interaction	* (= 0.030)	ns (= 0.919)	* (=0.039)	ns (=0.127)	* (=0.021)	ns (=0.328)
		Variety	‡ (<0.0001)	‡ (<0.0001)	‡ (<0.0001)	ns (=0.359)	ns (=0.239)	ns (=0.468)
		Fertilizer	‡ (<0.0001)	‡ (<0.0001)	ns (=0.06)	*(=0.049)	ns (=0.101)	ns (=0.503)
	Means within the column followed by different letter differed significantly by LSD (Student Newman-Keuls test at
	p<0.05);

*, †, ‡: significant at 0.05, 0.01 and 0.001 probability level; ns: not significant; IMG: immature green; MG: mature green; RR: red ripe.

Acknowledgments

Authors thank SCAC (Cameroon-France cooperation) for its financial help during fruits analysis. Special thank to Professors Mondher BOUZAYEN and Jean-Claude PECH, of UMR 990, INP-ENSAT/INRA of Toulouse, France for their help. Authors also thank M. Thomas MEKONTCHOU and Martin NGUEGUIM for their assistance during the field work at the Institute of Agricultural Research for Development of Foumbot.