Philippe Auger
email: auger@supagro.inra.fr

Alain Migeon
email: migeon@supagro.inra.fr

THREE NEW SPECIES OF TETRANYCHIDAE (ACARI, PROSTIGMATA) FROM THE FRENCH ALPS (SOUTH-EASTERN FRANCE)

Keywords: Acari, Tetranychidae, Bryobia cinereae n. sp., Bryobia mercantourensis n. sp., Eotetranychus quercicola n. sp., Bryobia longisetis comb. nov., ATBI, France

.

INTRODUCTION

All Taxa Biodiversity Inventories (ATBIs) are promoted to increase the knowledge about the biodiversity of particular areas. Samplings are carried out to achieve a baseline biodiversity assessment of fauna and flora and to provide ecological information on the distribution, abundance and biology of the species recorded. Within the framework of the second ATBI Mercantour-Alpi marittime [START_REF] Biaggi | Creating a biodiversity inventory in protected areas to increase knowledge of their natural heritage and to improve land management -Eco Mont[END_REF], promoted by the European Distributed Institute of Taxonomy (EDIT), the Mercantour National Park and the Muséum National d'Histoire Naturelle (MNHN), that took place in the Mercantour National Park located in the French Alps, in south-east of France, we have collected three new species of tetranychid mites. Among the forty-four species of Tetranychidae recorded from France only 6 are endemic from this country (Mi-geon andDorkeld, 2006-2013). Four of them belong to the genus Bryobia Koch, 1836, one to the genus Schizonobia Womersley, 1940 and one to the genus Eoetranychus [START_REF] Oudemans | -Acarologische Aanteekeningen CVII[END_REF] In the present work we report the description of two new species of Bryobia and a new Eotetranychus. According to the examination of morphological key-characters, Bryobia longisetis [START_REF] Reck | -Genus Bryobia Koch (Tetranychidae) described on the data material from Georgia -Soobshcheniya Akad[END_REF] is a new combination provided for the taxon previously known as Pseudobryobia longisetis.

MATERIALS AND METHODS

Mites were collected directly from field samples in 70 % ethyl alcohol. Following clearing in lactic acid (50 %) for 24 to 48 hours they were mounted in Hoyer's medium. The specimens were examined using a Leica DMLB phase contrast microscope and illustrated with the aid of a camera lucida. Measurements were performed using the imaging software Perfect Image® (Clara Vision) coupled with Pro-gres® Capture Pro 2.6 software for image acquisition. The setal nomenclature used in the description follows [START_REF] Lindquist | External anatomy[END_REF]. Legs setal count is given in the order: coxa, trochanter, femur, genu, tibia and tarsus. Numbers of setae refer to tactile setae, solenidia are given in parentheses and alternative counts are given in brackets. All measurements are given in micrometers and correspond to the holotype followed, in parentheses, by minimum and maximum values from paratypes. Setae are measured from theirs bases to their tips.

TAXONOMY

Family Tetranychidae Donnadieu, 1875

Subfamily Bryobiinae [START_REF] Berlese | Acarotheca Italica -Firenze[END_REF] Tribe Bryobiini [START_REF] Reck | Some fundamentals of the classification of the tetranychid mites -Soobshcheniya Akad[END_REF] Genus Bryobia Koch, 1836 Bryobia Koch, 1836:8-9;Pritchard and Baker, 1955:14;Wainstein, 1960:94;Tuttle and Baker, 1968: 4;Meyer, 1974:13. Type-species: Bryobia praetiosa Koch. Reck, 1947, comb. nov. Bryobia longisetis Reck, 1947, Soobshcheniya Akademii Nauk Gruzinskoi SSR, 8: 655 Type-species: female, Georgia (Gruziya), from Salvia nemorosa and Salvia sp. (Labiatae). Pseudobryobia longisetis [START_REF] Reck | -Genus Bryobia Koch (Tetranychidae) described on the data material from Georgia -Soobshcheniya Akad[END_REF], new combination. Wainstein, 1960, Trudy Nauchno-Issled. Inst. Zashchita Rastenii Kazakh., 5: 113 Since the reinstatement of the genus Pseudobryobia by Livshits and Mitrofanov (1972) and by Baker and Tuttle (1972), the main diagnostic characters that are listed in the diagnosis of this genus are the following: i) prodorsum without anterior projections over gnathosoma, ii) hysterosomal dorsocentral setae in the normal longitudinal dorsal position (f 1 setae in normal position, more or less aligned with first 3 pairs, not marginal), iii) coxal setal formula: 2-2-1-1.

Bryobia longisetis

According to the literature compiled we came to the conclusion that this species should not belong to the genus Pseudobryobia. First, the absence of prodorsal lobe over the gnathosoma can be questioned. In its original description, [START_REF] Reck | -Genus Bryobia Koch (Tetranychidae) described on the data material from Georgia -Soobshcheniya Akad[END_REF] reported that the outer prodorsal lobes are small but inners are cone-shaped almost fully fused. In the drawings of this species by Bagdasarian (1957), [START_REF] Reck | A key to the tetranychoid mites -Tbilissi: Akad[END_REF], [START_REF] Wainstein | -Tetranychoid mites of Kazakhstan (with revision of the family) -Trudy Nauchno-Issled[END_REF] and Livshits and Mitrofanov (1966), inner and outer prodorsal lobes are similar to those previously described by [START_REF] Reck | -Genus Bryobia Koch (Tetranychidae) described on the data material from Georgia -Soobshcheniya Akad[END_REF]: outer lobes are actually reduced to small tubercles but inner ones are coalescent into a tall cone with a small incision at the apex. Second, the dorsal pattern observed in this species does not correspond to that typical of the genus. Members of the fourth pair of hysterosomal dorsocentral setae (f 1 ) are never more or less in line with other dorsocentral setae. In the drawings of Bagdasarian (1957) and [START_REF] Reck | A key to the tetranychoid mites -Tbilissi: Akad[END_REF], f 1 setae are clearly located in marginal position, close (but not contiguous) to f 2 . In [START_REF] Wainstein | -Tetranychoid mites of Kazakhstan (with revision of the family) -Trudy Nauchno-Issled[END_REF] and [START_REF] Livshits | The mites of the genus Bryobia C.L. Koch[END_REF], f 1 setae are almost in marginal position, they are not in the normal longitudinal dorsal position and the distance between them is superior to that between f 2 setae. Finally, the coxal chaetotaxy does not fit with that of species belonging to the genus Pseudobryobia. In the descriptions of this species given by [START_REF] Wainstein | -Tetranychoid mites of Kazakhstan (with revision of the family) -Trudy Nauchno-Issled[END_REF] and by [START_REF] Livshits | The mites of the genus Bryobia C.L. Koch[END_REF], only one setae is present on the coxa II (coxal formula 2-1-1-1). Thus it is different to that of the genus Pseudobryobia and corresponds to that observed in the genus Bryobia. Although we did not had an opportunity to examine the holotype (or types), given the morphological characters cited above we consider that this species belongs to the genus Bryobia. ,3A). Outer propodosomal lobes small, about 10 µm, more or less similar in length to dorsal tubercles; inner lobes smaller about 5 µm (measured from the bottom of the incision between the inner lobes). Propodosomal lobes with basal width about 67 µm, distance between v 1 setae insertions about 13 µm. Incision between median lobes shallow. First pair of propodosomal setae (v 1 ) less than half the size of the second pair (v 2 ). A horizontal line joining tip of v 1 setae located on the inner lobes crosses v 2 setae about their three-quarters. Dorsal body setae not spatulate, elongated, moderately stout, serrate, inserted on tubercles, subequal in length with the exception of v 1 setae far smaller (Figs. 1A,1B). Dorsocentral setae (c 1 , d 1 and e 1 ) shorter than distances between consecutive setae (length of holotype and variations of three paratypes): v 1 17 (14 -15); v 2 40 (38 -42) 47 (53 -55). Distances between setae: c 1c 1 52 (54 -55), d 1 -d 1 33 (31 -32), e 1 -e 1 17 (20 -24), c 1 -d 1 85 (87 -99), d 1 -e 1 60 (64 -66). Sacral setae (f 1 and f 2 ) in marginal position and contiguous. Dorsal surface wrinkled, on propodosoma irregular medially and mostly longitudinal laterally, transverse on hysterosoma, more or less arched in the distal part comprised between e 3 and h 1 setae. Area immediately anterior to h 1 setae with fine arched longitudinal reticulation.

Bryobia cinereae

Gnathosoma -Stylophore rounded, slightly indented (slightly emarginate anteriorly), longer than wide. Tibial claw of palpus bidentate (Fig 3B). Palptarsus slightly elongated, about 18.5 (19.2) long with six setae and one solenidion. Eupathidia ul'ζ, ul"ζ slightly shorter than suζ, solenidion short. Peritreme anastomosed distally in a relatively long and slender enlargement: length 33 (26 -28), width 8.5 (9 -10) (Fig. 3C).

Venter -Striation transverse between 1 st (1a) and 2 nd (3a) pairs of setae becoming irregularly longitudinal (broken medially) between 2 nd and 3 rd (4a) pairs of setae and transverse between 3 rd and aggenital (ag) pairs of setae. Area immediately anterior to genital flap with irregular longitudinal striation, V-shaped between ag setae (Fig. 3D). Sacculus of spermatheca oblong (shape variations due to mounting) (Fig. 3E). Three anal and two para-anal setae.

Legs -Length inferior to body length. µm long (length of holotype and variations of three paratypes, measured from trochanter to tarsus), . Length of segments of leg I as follows: trochanter 21 (22 -24), femur 99 (90 -96), genu 49 (49 -53), tibia 61 (62 -66), tarsus 75 (75 -79). Leg setal count as follows (Figs. 2A-D

): I 2 -1 -8 [9] -4 -8 [7] + (1) -17[16] + (2) + 2 duplexes; II 1 -1 -5 -4 -3 [5] -14[13] + (2) + 1 duplex; III 1 -1 -4[3] -2 [3] -4[5] -12[11] + 1 duplex; IV 1 -1 -4 [3]-2 -4 [3-5] -13[12-14] + (1).
Tarsus III associated setae serrate and approximate with solenidion forming duplex, the tactile member slightly longer and proximal (Fig. 2E); tarsus IV with solenidion well-separated from tactile, proximal, about one third the length of tactile (Fig. 2F). True claws uncinate, with one pair of tenent hairs, empodial pads each bearing two rows of tenent hairs (Fig. 2G). Etymology -The specific epithet cinereae refers to the species name of the host plant on which mites were collected.

Male: Unknown

Remarks -The combination of prodorsal lobes poorly developed and dorsal setae not spatulate but elongate with dorsocentral setae inferior in length to the distance between consecutive setae brings this species close to B. sarothamni Geijskes, (1939) Diagnosis -With four long setae present on the interior dorsal row of femur I this species belongs to the berlesei-group [START_REF] Eyndhoven | L'interprétation de Bryobia speciosa Berl. (non Koch) -Notulae ad Tetranychidas 4[END_REF][START_REF] Eyndhoven | -The berlesei-Group of the genus Bryobia Koch (Acari, Tetranychidae)[END_REF]. Empodial pad of leg I with a pair of tenent hairs others with two rows of tenent hairs, inner propodosomal lobes are well separated and more or less cone-shaped with large fused base, outer lobes smaller and cone shaped, dorsal setae inserted in small tubercles, spatulate with sacral and clunal setae slightly longer.

Description:

Female: Holotype 600 µm long (excluding gnathosoma, from the tip of v 1 to the tip of h 1 ), width 350 µm. Ten paratypes measured, 540 -595 µm long, width 310 -360 µm. Dorsum -Prodorsum with four pairs of setae and with developed anterior lobes (Figs. 4A,6A). Outer propodosomal lobes rather low, conical, not extending beyond medial of inner lobes. Medial projection well expanded, inner lobes well separated in their distal part with variable obvious incision, 13 (7 -13) µm in depth (measured from the bottom of the incision between the inner lobes).

A horizontal line joining tip of v 2 setae located on the outer lobes crosses v 1 setae about their base, v 1 about two-thirds the size of v 2 . Dorsal body setae spatulate, inserted on tubercles, subequal in length with the exception of v 1 setae far smaller, sacrals (f 1 , f 2 ) and clunals (h 1 ) somewhat longer (Figs. 4A,B). Gnathosoma -Stylophore longer than wide. Tibial claw of palpus bidentate. Palptarsus elongated, about 24 (20.5 -25) long with three tactile setae, three eupathidia and one solenidion (Fig. 6B). Eupathidia ul'ζ, ul"ζ slightly inferior to suζ in length, solenidion shorter. Peritreme anastomosed distally in a relatively long and slender enlargement (Fig. 6C): length 33 (31 -40), width 8 (6.5 -8.5).

Venter -Striation transverse between 1 st (1a) and 2 nd (3a) pairs of setae, absent (rare irregularly folds may be present) between 2 nd and 3 rd (4a), longitudinal between members of 4a setae and transverse between 3 rd and aggenital (ag) pairs of setae. Area immediately anterior to genital flap with irregular longitudinal striation. Sacculus of spermatheca oval shaped (Fig. 6D). Three anal and two para-anal setae present. IV 1 -1 -5 -6 -9 -14 + (1). Internal dorsal row on femur I with four long setae (from proximal to distal setae) and one normal setae: 43 (41 -47), 51 (47 -53), 41 (39 -46) and 42 (38 -41) µm in length. Tarsus III associated setae serrate and approximate with solenidion forming duplex, the tactile member longer and proximal (Fig. 5D) -length of solenidion 14 (12.5 -15), length of tactile 20 (16 -20); tarsus IV with solenidion wellseparated from tactile, short and proximal (Fig. 5E) -length of solenidion 9 (8 -9.5), distance between solenidion and tactile 6.5 (4.5 -6.5). True claws uncinate, claw and empodium I with one pair of tenent hairs, other claws with two pairs and other empodial pads each provided with two rows of tenent hairs (Figs. 5F,G). Deutonymph (Figs. 7A,B): Dorsum -Prodorsal lobes developed, conical in shape, inner lobes less separated as in female, prodorsal setae v 1 and v 2 spatulate and serrate, v 2 the largest almost twice the length of v 1 setae; a horizontal line joining the tips of v 2 setae also nearly passes the tips of v 1 setae. Dorsal body setae inserted on tubercles (stronger in posterior area), spatulate excepted the third pair of dorsolateral setae (e 3 ), sacrals (f 1 , f 2 ) and clunals (h 1 ), gradually longer, narrower and pectinate. Dorsocentral setae, c 1 , d 1 and e 1 , shorter than distances between consecutive setae. dorsal row on femur I with two long setae and one normal seta. Leg setal count as follows: I 2 -1 -8 -4 -9 + (1) -14 + (1) + 2 duplexes; II 1 -1 -6 [5] -4 -5 -11 + 1 duplex; III 1 -1 -2 -3 -5 -10 + (1); IV 1 -0 -2 -3 -5 -10.

True claws uncinate with one pair of tenent hairs, empodia provided with two rows of tenent hairs, empodial pad of empodium I shorter.

Protonymph (Figs. 7C,D): Dorsum -Prodorsal lobes weakly developed, tubercle like, v 1 very short, spatulate and serrate with spiky appearance, v 2 larger, spatulate and serrate. Other dorsal body setae spatulate with the exception of e 2 sub-spatulate and the following (e 3 , f 1 , f 2 and h 1 ) elongate, serrate and larger. Lengths of dorsal setae (variations of 4 protonymphs): v 1 7 -11; v 2 23 -28; sc 1 22.5 -25.5; sc 2 18.5 -20.5; c 1 18 -21; c 2 17 -18.5; c 3 16 -18.5; d 1 16 -18.5; d 2 20 -23; d 3 21 -31; e 1 18 -22; e 2 27 -31; e 3 33 -36; f 1 32 -36; f 2 34 -37; h 1 35 -37. Setae f 1 and f 2 in marginal position.

Legs -Length inferior to body length. Internal dorsal row on femur I with one long seta and one normal seta. Leg setal count as follows: I 2 -1 -3 -4 -5 + (1) -10 + (2) + 2 duplexes; II 1 -0 -3 -4 -5 -9 + 1 duplex; III 1 -0 -2 -2 -5 -8; IV 0 -0 -2 -2 -5 -6. True claws uncinate with one pair of tenent hairs, empodia with two rows of tenent hairs. Larvae (Figs. 7E,F): Dorsum -Prodorsal lobes absent, v 1 very short and rod like, v 2 long, serrate, inserted on small tubercles. Dorsal body setae elongate, serrate, setae e 3 to h 1 the largest. Lengths of dorsal setae (variations of 4 larvae): v 1 5 -7; v 2 23 -25; sc 1 18 -22; sc 2 18 -20; c 1 19 -24; c 2 17 -19; c 3 13 -16; d 1 19 -24; d 2 18 -22; d 3 24 -29; e 1 23 -28; e 2 25 -31; e 3 32 -36; f 1 35 -39; f 2 35 -40; h 1 35 -37. Seta f 1 in normal position.

Legs -Length inferior to body length. Internal dorsal row on femur I with one long seta and one normal seta. Leg setal count as follows: I 1 -0 -3 -4 -5 + (1) -7 + 1 duplex; II 0 -0 -3 -4 -5 -7 + 1 duplex; III 0 -0 -2 -2 -5 -6.

True claws uncinate with one pair of tenent hairs, empodia with two rows of tenent hairs.

Remarks -In addition to the four long setae present on the interior dorsal row of femur I, as this species bears one pair of tenent hairs on the empodium I, B. mercantourensis is close to B. provincialis [START_REF] Eyndhoven | L'interprétation de Bryobia speciosa Berl. (non Koch) -Notulae ad Tetranychidas 4[END_REF][START_REF] Eyndhoven | -The berlesei-Group of the genus Bryobia Koch (Acari, Tetranychidae)[END_REF]B. dikmenensis Eyndhoven and[START_REF] Eyndhoven | -The berlesei-Group of the genus Bryobia Koch (Acari, Tetranychidae)[END_REF] that belong to the berlesei-group [START_REF] Eyndhoven | L'interprétation de Bryobia speciosa Berl. (non Koch) -Notulae ad Tetranychidas 4[END_REF][START_REF] Eyndhoven | -The berlesei-Group of the genus Bryobia Koch (Acari, Tetranychidae)[END_REF]. This species is clearly smaller in length and width than B. provincialis and the first leg is also obviously longer in the latter. Conversely, B. mercantourensis is slightly longer and obviously broader than B. dikmenensis and the second, third and fourth pairs of legs are shorter in the latter. It is mainly distinctive from B. provincialis and B. dikmenensis by the shape of the propodosomal lobes: mammelliform with inner lobes largely fused in the latter whereas conical and well separated in B. mercantourensis. The latter can also be separated from B. provincialis by differences in shape of deutonymph's dorsohysterosomal setae e 3 and f 1 , subspatulate vs. elongate and narrow in B. provincialis and B. mercantourensis respectively. Legs chaetotaxy also clearly differs between the deutonymphs of these two species. Bryobia dikmenensis can be distinguished from B. mercantourensis and from B. provincialis by the reduced size of its second and third pairs of dorsocentral setae (d 1 and e 1 ) in comparison with other dorsohystersomal setae. Several characters found in juveniles of B. dikmenensis and of B. mercantourensis can also be used to separate them: the ratio between larval v 1 and v 2 setae is two-fold higher (4 vs. 2) in B. mercantourensis; protonymphal prodorsal lobes in B. dikmenensis ressemble that of female whereas they are almost absent (weakly developed) in B. mercantourensis.

Etymology -The species designation mercantourensis is named after the location where the specimens were found: in the Mercantour French National Park.

Subfamily Tetranychinae Berlese, 1913

Tribe Tetranychini [START_REF] Reck | -Spider mite fauna from Georgia (Tetranychidae: Acarina) -Trudy Zoologicheskogo Instituta Akademia[END_REF] Genus EotetranychusOudemans, 1931 Eotetranychus Oudemans, 1931:224;Pritchard and Baker, 1955:138;Gutierrez, 1967:370;Tuttle and Baker, 1968:85;Meyer, 1974:189;Tuttle, Baker and Abatiello, 1976:37;Meyer, 1987:110. Schizotetranychus (Eotetranychus), Wainstein , 1960: 178;Mitrofanov, Strunkova and Livshits, 1987: 90 (subgenus) Type-species: Trombidium tiliarium Hermann.

Eotetranychus quercicola n. sp. (Figures 8-13)

Type-specimens -Holotype (male), 7 male, 20 female and one deutonymph paratypes on 26 preparations from Quercus pubescens Willd., (Fagaceae), cime de Braus (43.875°N 7.394°E, alt. 1040 m), Lucéram, France, 23-VII-2009, leg. P. Auger. All the material housed in the INRA collection of the CBGP, coll. Auger-Migeon N°1807 for holotype and 1788-1814 for paratypes.

Diagnosis -Dorsohysterosomal setae longer than the intervals between their bases, genital area provided with a genital flap and the area anterior to it bearing a transverse striation design. End of peritreme straight, bulbous, posteriorly enlarged. Male aedeagus long and slender, flagellate and undulate near the middle.

Description:

Male: Holotype 338 µm long (without gnathosoma), gnathosoma 92 µm long. Seven paratypes measured, 325 -361 µm long, gnathosoma 84 -91 µm long. Dorsum -Dorsal body setae long, linear lanceolate, well surpassing in length distance between consecutive bases (length of holotype and variations of seven paratypes): v 1 49 (44 -49); sc 1 (79 -84); sc 2 55 (50 -55); c 1 70 (66 -72); c 2 68 (68 -72); c 3 60 (57 -61); d 1 60 (60 -63); d 2 73 (64 -74); e 1 52 (53 -59); e 2 66 (64 -72); f 1 47 (47 -51); f 2 30 (28 -39); h 1 26 (23 -26). Dorsal striation with rounded lobes on propodosoma and hysterosoma up to third row of dorsal setae (e).

Gnathosoma -Palptarsus terminal sensillum about 3 -3.5 as long as broad (length of holotype and variations of four paratypes): 5.6 (5 -5.6) long 1.6 (1.6 -1.7) wide, solenidion 3.7 (3.8 -4) µm long, lateral eupathidia asymmetrical, ul"ζ longer than ul'ζ: 8.7 (8.4 -9.1) µm and 5 (4.5 -5) µm respectively (Fig. 10B). Peritreme straight, bulbous distally. Distal enlargement asymmetrical, more developed posteriorly, club-shaped, varying in size and shape among and between specimens (Fig. 10A).

Venter -Ventral striae without lobe.

Legs -Length inferior to body length, leg I 165 (165 -169) µm long (from trochanter to tarsus, holotype and variations of seven paratypes), leg . Length of segments of leg I as follows (Figs. 9A,B): trochanter 18 (18 -21), femur 50 (48 -52), genu 22 (22 -25), tibia 29 (30 -31), tarsus 46 (42 -45). Leg setal count as follows (Figs. 9A,B): I 2 -1 -10 -5 -9 + (4) -13 + (3) + 2 duplexes; II 2 -1 -7 -5 -8 -13 + (1) + 1 duplex; III 1 -1 -4 -4 -6 -10 + (1); IV 1 -1 -4 -4 -7 -10 + (1). Tarsus I with distal duplex solenidion longer than that of proximal duplex: 49 -56 µm and 33 -36 µm respectively. Tactile members of distal and proximal duplexes subequal in length 10 -12 µm and 11 -13 µm respectively. Tarsus II sensory member of duplex 26 -30 µm long and tactile 10 -12 µm. Tarsus II with dorsal proximal solenidion slightly longer 13 (13.5 -15 µm) than distance with duplex setae 12 (12 -13 µm). Solenidia of tarsi III and IV shorter than distances to distal tactiles: length of solenidia III and IV 13.5 -15.5 and 15 -16 µm -distance between solenidia and tactiles 14.5 -20 and 18 -20 µm. Empodium I bifid each side composed of three digits, medial digit the stoutest, strong, ventral and dorsal digits slender and shorter (Fig. 9C). Empodia II-IV split into three pairs of hairs with proximoventral pair stronger and with ancillary setae (Fig. 9D). No dorsomedian spur observed.

Aedeagus -Long, slender, acutely tapering and strongly undulate near the middle, 32 (29 -32.5) µm in length (Fig. 10C). 51,d 1 -1 44 51. Hysterosomal striation transverse, dorsal hysterosomal striae with small mostly broader than tall, rounded to triangularly rounded without oblong lobes (Fig. 8B). Prodorsal lobes on striation rounded and broader than tall.

Female

Gnathosoma -Palptarsus terminal sensillum about 2 -2.3 as long as broad, 6.6 -6.9 µm long 3.1 -3.3 µm wide (variations of six paratypes), solenidion 3.7 -4.2 µm long, lateral eupathidia asymmetrical, ul"ζ longer than ul'ζ: 9 -9.6 µm and 5.2 -6.2 µm respectively (Fig. 12B). Peritreme as in male (Fig. 12C).

Venter -Area immediately anterior to genital flap with transverse striae, genital flap with transverse slightly arched striae typical of willametteigroup [START_REF] Pritchard | A revision of the spider mite family Tetranychidae -San Francisco[END_REF] (Fig. 12D). Lobes on ventral striation present laterally between third pair of ventral setae (4a) and aggenital pair (ag), rare poorly developed lobes may be present between members of 4a, anteriorly and posteriorly. Two pair of para-anal and two pairs of anal setae. spectively), slightly shorter than distances between their bases and those of distal tactile setae: distance between solenidia III and IV and tactile setae 21 -26 and 21 -27 µm respectively. Empodia I-IV split into three pairs of hairs with proximoventral pair stronger and with ancillary setae. No dorsomedian spur observed (Fig. 12A).

Remarks -With dorsal setae longer than the intervals between them and eight tactile setae on tibia II this species is assigned into the tiliarium group [START_REF] Pritchard | A revision of the spider mite family Tetranychidae -San Francisco[END_REF], its genital area pattern corresponds to the willamettei species group [START_REF] Tuttle | -Spider mites of Mexico (Acarina : Tetranychidae) -Internat[END_REF][START_REF] Baker | -A guide to the spider mites (Tetranychidae) of the United States[END_REF] and because of the shape of its aedeagus this species belongs to the carpini-species group [START_REF] Pritchard | A revision of the spider mite family Tetranychidae -San Francisco[END_REF][START_REF] Ehara | Four Species of the carpini Complex of Eotetranychus in Japan (Acarina : Tetranychidae) -The Journal of the Faculty of Education[END_REF][START_REF] Baker | -A guide to the spider mites (Tetranychidae) of the United States[END_REF]. Among members of Eotetranychus from this group it can be easily distinguished by the shape of the distal peritremal enlargement: neither bent nor hooked but straight and bulbous, asymmetrical and posteriorly enlarged. Eotetranychus quercicola shares this character with only one species belonging to the carpini group: E. colurnae [START_REF] Mitrofanov | -New species of Schizotetranychus mites (Acarina, Tetranychidae) from the Crimea -Nauch[END_REF] However, E. quercicola can be separated from this species by several obvious characters: for example dorsal setae, duplex solenidia, legs and distances between dorsocentral setae insertions are shorter in E. colurnae (see Table I). The aeadeagus is longer in E. colurnae and male eupathidia ul'ζ and ul"ζ are asymmetrical in male of E. quercicola only. Eotetranycus querci [START_REF] Reeves | Tetranychidae infesting woody plants in New York State, and a life history study of the elm mite Eotetranychus matthyssei n[END_REF], that also belongs to the carpinispecies group can be found on oak and birch in the USA [START_REF] Reeves | Tetranychidae infesting woody plants in New York State, and a life history study of the elm mite Eotetranychus matthyssei n[END_REF] and on Tilia japonica (Miq.) Simonk. in Japan [START_REF] Ehara | Four Species of the carpini Complex of Eotetranychus in Japan (Acarina : Tetranychidae) -The Journal of the Faculty of Education[END_REF]. It can be easily separated from E. quercicola by the shape of its peritreme (slightly bent to almost U-shaped) and by its aedeagus which is weakly undulate near the middle.

Etymology -the species designation, quercicola, refers to the host plant on which mites were collected meaning inhabits oak.

Biological observations -Adults of this species are yellowish green in colour (Fig. 13). This species lives on the under surface of the leaves, produces webbing that delimits small colonies more or less oval-shaped.

FIGURE 1 :

 1 FIGURE 1: Bryobia cinereae n. sp., female: A -dorsal aspect; B -dorsal h 1 seta.

FIGURE 2 :

 2 FIGURE 2: Bryobia cinereae n. sp., female: A -tarsus and tibia I; B -genu and femur I; C -tarsus and tibia II; D -genu and femur II; Eduplex setae on tarsus III; F -solenidion and associated tactile seta on tarsus IV; G -empodia I-IV.

FIGURE 3 :

 3 FIGURE 3: Bryobia cinereae n. sp., female: A -prodorsal lobes; B -Palpal tibia and tarsus; C -peritremal distal anastomosis; Danterogenital striation; E -spermatheca (variations between preparations).

FIGURE 4 :FIGURE 5 :FIGURE 6 :

 456 FIGURE 4: Bryobia mercantourensis n. sp., female: A -dorsal aspect; B -dorsal c 1 seta.

  Legs Length (femur-genu-tibia-tarsus) inferior to body length, leg I 419 (390 -410) µm long and (length holotype of leg II 235 (215 -230), leg III 240 (220 -240), leg IV 260 (250 -280). Length of of I as follows: femur 155 (135 -150), genu 70 (65 -70), tibia 100 (84 -105), tarsus 93 (85 -99). Leg setal count as follows (Figs. 5A, B,

FIGURE 7 :

 7 FIGURE 7: Bryobia mercantourensis n. sp.: A -deutonymphal prodorsal lobes; B -deutonymphal dorsal hysterosomal distal part; Cprotonymphal prodorsal lobes; D -protonymphal dorsal hysterosomal distal part; E -larval prodorsal anterior part; F -larval dorsal hysterosomal distal part.

FIGURE 10 :

 10 FIGURE 10: Eotetranychus quercicola n. sp., male: A -variations in the distal end of the peritreme (superposed peritremes belong to the same mite, juxtaposed one are variations between mites); B -palptarsus; C -aedeagi.

FIGURE 11 :

 11 FIGURE 11: Eotetranychus quercicola n. sp., female: A -tarsus and tibia B -tarsus and tibia II.

FIGURE 12 :

 12 FIGURE 12: Eotetranychus quercicola n. sp., female: A -empodia I-IV; B -palptarsus; C -distal part the peritreme (variations between individuals); D -flap and anterogenital area.

FIGURE 13 :

 13 FIGURE 13: Eotetranychus quercicola n. sp., female.

  , B.longisetis Reck, (1947), B. artemisiae[START_REF] Bagdasarian | Contributions to the fauna of spider mites (fam. Tetranychidae) of Yerevan and its environs -Akad[END_REF]), B. variabilis Manson, (1967) and B. serifiotica[START_REF] Hatzinikolis | -Bryobia serifiotica n. sp. (Acari: Tetranychidae: Bryobiinae) from Greece -Internat[END_REF]. It can be distinguished from B. sarothamni by the dorsal hysterosomal setae which are lanceolate, shorter, variable in length (h 1 the largest) versus elongate and subequal in length in B. cinereae. Bryobia longisetis can be separated from B. cinereae by the propodosomal inner projection which is more developed, by medial lobes which are almost fused, by f 1 and f 2 dorsal setae which are not contiguous and by a different leg chaetotaxy. In B. variabilis (the form bearing long, slender and serrate dorsal setae) the leg setal formula is different and dorsal setae are slender, variable in length (c 2 , c 3 , d 1 and d 2 being much smaller) whereas quite stout and subequal in length in B. cinereae. Bryobia serifiotica differs from B. cinereae by the dorso hysterosomal setae larger in the latter, by the difference in size of the vertical setae (v 1 slightly inferior to v 2 in B. serifiotica vs. v 1 up to three times smaller than v 2 in B. cinereae), by the position of f 1 setae (more or less in normal position and well separated in B. serifiotica whereas f 1 and f 2 are in marginal position and contiguous in B. cinereae) and by the solenidion of the tarsus IV (associated with a tactile setae but well separated and proximal in B. serifiotica and B. cinereae respectively). Depending on the literature referred, B. artemisiae is more or less close to B. cinereae. In the original description by[START_REF] Bagdasarian | Contributions to the fauna of spider mites (fam. Tetranychidae) of Yerevan and its environs -Akad[END_REF], prodorsal lobes are similar in the two species but vertical setae (v 1 and v 2 ) are subspatulate to spatulate (elongate in B. cinereae) and dorsal hysterosomal setae are short and fan-shaped (elongate in B. cinereae). According to[START_REF] Reck | A key to the tetranychoid mites -Tbilissi: Akad[END_REF], dorsal setae of B. artemisiae vary from short spatulate to slightly elongate and, in his drawing, v 1 and v 2 setae are elongate and lanceolate (only elongate in B. cinereae).[START_REF] Wainstein | -Tetranychoid mites of Kazakhstan (with revision of the family) -Trudy Nauchno-Issled[END_REF] mentions that dorsal setae are narrowly spatulate and almost elongated. InLivshitz and Mitofanov (1971) and[START_REF] Mitrofanov | -Keys to the tetranychid mites (Tetranychidae, Bryobiidae) fauna of the USSR and adjacent countries -Dushanbe: Donish[END_REF] the drawing of B. artemisiae in habitus resembles to B. cinereae: prodorsal lobes are small, v 2 are longer than v 1 , dorso hysterosomal setae are elongate and inserted on tubercles. However, v 1 and v 2 setae are spatulate and subspatulate (narrow in B. cinereae), dorso central setae (c1, d 1 , e 1 ) are longer to dorso lateral (similar in length in B. cinereae) and the leg setal count is different.

	Bryobia mercantourensis n. sp.
	(Figures 4-7)
	Type-specimens -Holotype (female), 15 female
	7 deutonymhs, 4 protonymphs and 7 larvae
	paratypes on 30 microscopic preparations from
	Genista cinerea (Vill.) DC. (Leguminosae), cime de
	Braus (43.875°N 7.394°E, alt. 1040 m), Lucéram,
	France, 23-VII-2009, leg. P. Auger. All the material
	deposited in the INRA collection of the CBGP, coll.
	Auger-Migeon N°1758 for holotype, 1759-1787 for
	paratypes.
	Other material examined -Ten females on 8
	microscopic preparations from G. cinerea, Pont du
	commun (43.985°N 7.547°E, alt. 450 m), Saorge,
	France, 21-VII-2009, leg. P. Auger, coll. Auger-
	Migeon N°1700-1707.

TABLE 1 :

 1 Comparison between some morphological characters of Eotetranychus colurnae and Eotetranychus quercicola n. sp. (lengths are given in micrometers).

	E. colurnae*	E. quercicola

http://www1.montpellier.inra.fr/CBGP/acarologia/ ISSN 0044-586-X (print).ISSN 2107-7207 (electronic)

ACKNOWLEDGEMENTS

The study was founded by the European Distributed Institute of Taxonomy (EDIT) with the collaboration and financial support of the Mercantour National Park. We are grateful to M.-F. Leccia (ATBI Mercantour Project Manager) and O. Gargominy (Muséum National d'Histoire Naturelle, Paris) who initiated and promoted this project. Dr Tea Arabuli (Agrarian University of Georgia) is also deeply acknowledged for providing the translation of the species description of E. colurnae (in Russian).